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The map label for this gene is nth [H]

Identifier: 229587104

GI number: 229587104

Start: 1069243

End: 1069875

Strand: Direct

Name: nth [H]

Synonym: RAF_ORF1047

Alternate gene names: 229587104

Gene position: 1069243-1069875 (Clockwise)

Preceding gene: 229587094

Following gene: 229587105

Centisome position: 83.63

GC content: 33.49

Gene sequence:

>633_bases
ATGCAAGCACAAATAGTTAATAAAATTTTTGAGATTTTTAGCAAGAATAATCCCAATCCGAAAACCGAATTAATATATAA
GAATGATTTTACCTTATTAGTAGCGGTAATATTATCTGCTCAAGCCACCGATATATCAGTAAATTTAGCAACTAAATCTT
TATTTGCAACTTATGATACACCAGAAAAAATTTTAGAACTCGGCGAAGAAGGACTTAAAAAATATATACAATCTATAGGA
CTATTTAACAGCAAAGCAAAAAATATTATCGCATTATGTAAAATATTGATAAGTAATTATCAAGCTTCAGTACCGAATGA
TTTTAAGGCATTAATTAAACTACCGGGCGTTGGCAGAAAAACTGCTAATGTTGTACTAAATTGCTTATTCGGCATGCCTA
CAATGGCAGTTGATACGCATGTCTTTAGAGTAGCCAACAGAATCGGGCTTGCTAAAGGCGGTACTCCTGAAATAGTAGAA
AAAGAGCTACTGCAGATTATCGATACAAAATGGCTAACGCATGCCCATCACTGGCTAATTCTGCACGGCAGATATATATG
CAAAGCTAGAAAACCTGATTGCGACATCTGCCCTATTAAAGAGTATTGCGATTATTATTTTAACCTTAAATAA

Upstream 100 bases:

>100_bases
ATTATTTTTTATCTCATTTTCTATAAATTTAAAATTTTTATTTTCTAGCATTTTAAGACTCAATAATTTATCTTCTAAAA
TACACTTTCTATAGCAATAT

Downstream 100 bases:

>100_bases
GAGTTATAGTAAATCTTATAAATTACCAATTATTTCTGAATAATATTCATGTTCTATAGAATTAGGTTTAGGATCAAGTG
TTGCTAATGTTTCTAAATGA

Product: Endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MQAQIVNKIFEIFSKNNPNPKTELIYKNDFTLLVAVILSAQATDISVNLATKSLFATYDTPEKILELGEEGLKKYIQSIG
LFNSKAKNIIALCKILISNYQASVPNDFKALIKLPGVGRKTANVVLNCLFGMPTMAVDTHVFRVANRIGLAKGGTPEIVE
KELLQIIDTKWLTHAHHWLILHGRYICKARKPDCDICPIKEYCDYYFNLK

Sequences:

>Translated_210_residues
MQAQIVNKIFEIFSKNNPNPKTELIYKNDFTLLVAVILSAQATDISVNLATKSLFATYDTPEKILELGEEGLKKYIQSIG
LFNSKAKNIIALCKILISNYQASVPNDFKALIKLPGVGRKTANVVLNCLFGMPTMAVDTHVFRVANRIGLAKGGTPEIVE
KELLQIIDTKWLTHAHHWLILHGRYICKARKPDCDICPIKEYCDYYFNLK
>Mature_210_residues
MQAQIVNKIFEIFSKNNPNPKTELIYKNDFTLLVAVILSAQATDISVNLATKSLFATYDTPEKILELGEEGLKKYIQSIG
LFNSKAKNIIALCKILISNYQASVPNDFKALIKLPGVGRKTANVVLNCLFGMPTMAVDTHVFRVANRIGLAKGGTPEIVE
KELLQIIDTKWLTHAHHWLILHGRYICKARKPDCDICPIKEYCDYYFNLK

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=177, Percent_Identity=30.5084745762712, Blast_Score=79, Evalue=2e-15,
Organism=Escherichia coli, GI1787920, Length=198, Percent_Identity=55.5555555555556, Blast_Score=231, Evalue=4e-62,
Organism=Caenorhabditis elegans, GI17554540, Length=175, Percent_Identity=33.1428571428571, Blast_Score=93, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6324530, Length=193, Percent_Identity=27.979274611399, Blast_Score=70, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6319304, Length=183, Percent_Identity=29.5081967213115, Blast_Score=67, Evalue=2e-12,
Organism=Drosophila melanogaster, GI45550361, Length=180, Percent_Identity=31.1111111111111, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 23658; Mature: 23658

Theoretical pI: Translated: 9.16; Mature: 9.16

Prosite motif: PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAQIVNKIFEIFSKNNPNPKTELIYKNDFTLLVAVILSAQATDISVNLATKSLFATYDT
CCHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHCCCCCEEEEEHHHHHHCCCCC
PEKILELGEEGLKKYIQSIGLFNSKAKNIIALCKILISNYQASVPNDFKALIKLPGVGRK
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCH
TANVVLNCLFGMPTMAVDTHVFRVANRIGLAKGGTPEIVEKELLQIIDTKWLTHAHHWLI
HHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCEEEE
LHGRYICKARKPDCDICPIKEYCDYYFNLK
EECEEEEECCCCCCCCCCHHHHHHHHCCCC
>Mature Secondary Structure
MQAQIVNKIFEIFSKNNPNPKTELIYKNDFTLLVAVILSAQATDISVNLATKSLFATYDT
CCHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHCCCCCEEEEEHHHHHHCCCCC
PEKILELGEEGLKKYIQSIGLFNSKAKNIIALCKILISNYQASVPNDFKALIKLPGVGRK
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCH
TANVVLNCLFGMPTMAVDTHVFRVANRIGLAKGGTPEIVEKELLQIIDTKWLTHAHHWLI
HHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCEEEE
LHGRYICKARKPDCDICPIKEYCDYYFNLK
EECEEEEECCCCCCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA