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The map label for this gene is proP4 [H]

Identifier: 229587043

GI number: 229587043

Start: 982784

End: 984172

Strand: Direct

Name: proP4 [H]

Synonym: RAF_ORF0953

Alternate gene names: 229587043

Gene position: 982784-984172 (Clockwise)

Preceding gene: 229587039

Following gene: 229587045

Centisome position: 76.87

GC content: 35.71

Gene sequence:

>1389_bases
ATGAATAATGCATTAAGTAAAAGGGATTTTTCGATCCTAATCGGTAATGCGATGGATCATTTCGATACTGCACTTTATGG
GTTTCTTGCTCCGCTACTTGCTGGTATTTTTTTTCCGAATCATGACAAAGTCGTTGCTTTAATACTTACCTATAGTGTTC
TTGCTACGTCTTTATTTACACGTCTCATAGGCTCTTATTTTTTTGGCGTAATTGCTAAAAAATACGGCGGTGTTTTTGCT
TTATCTTACTCTTTAATCGGCGTTGCTTTAACAACTAGCCTAATCGGTTTAATACCTTCACATGCTCAAATAGGCTGGCT
TGCACCGCTATTATTAGTGTTGCTTAGAACGTTGCAAGGAGTATATTCCGAGGGTGAATGTGCCATTGCTAAATTATTTA
TTTTAGAGAATAAAGAAGAAAAAAAAGCGCTTAAAGCTTCTTACCTTTATCAAACATCTACTATGGTCGGTATTATTCTT
GCTTCATTTATCAGTACTATAGTTTTAAATGTAGAGTATAATACATATTGGCGTTTATGTTTCATATTTGGTGGACTTAC
AGCACTGATAGGTTATTTTTTAAGAAAGCGCGAGGATATTGTAGTCAAGCCCTCATTTTCATCCCGCGGCTTGACCACGG
GATCCAATAAAAAAGATAAAATTACTTTACGTAATTTTTGGATACTGTGGTCAAGCCACGGGATGACACCTGGGTCCTCT
CCTCGCAATGACGTAGTATCATCACTATTACAAGACTTAACTACAATTTGGAACTGTAAACTAAGTATCTTACGCATTAG
TTTTGCCACAGGTTTCTCATATATGACTTATATAGTACCTTTCGTCTTTTTGAATAGTTTTATTCCGCTTATCACTGATA
TATCACTCGAAATAATGATGAAATTTAATACTGAGTTTCTGATTTTTGATATGATTATGATTCCGATAATAGGGCATCTA
ACAAAAAAGCTACATTACCTTAAAATACTAAACGGTACTCTTATTTTAATGAGTTTAAGCATAATTCCTTTATGGCTCTG
CTTGAATAATGCATCTATATGGTATGTTAATTTTGTACGTATTTGGATTATAATACTCGGTGTCGGGTTCTTGGCACCTT
TGAACTGCTGGCTAAATGATTTATTTAAAACTGCCGATAAATATATGTTAGTCGGTATCGGCAGTAGTATAGGTGCTTCA
TTAATCGGACGTCTTACTCCGTCAATCTGCCTAATGCTATGGTATGTAACCAGCAACTCTTTATCAATTGCGGTTTATAT
AACCGCAATATCGATGGTCACTTTATGGGCTGTTAGAGGAGTTGTTGCACGGGTCAATGTCATTCCTGCGAAAGCAGGAA
TGACATCAAGAACATTATCTAATCCCTAA

Upstream 100 bases:

>100_bases
GTATATTAAACGTTCAGCATCCGAGGACTTACAAAGACATTGTCACCAATGCTCCAAATTCATCGAGTATAATCCATTAT
AAATTTTAAATTATTAATAT

Downstream 100 bases:

>100_bases
CAAGACTGTTATTAGGAAAAGAATTTAATAAAACACCATACTCAAAACTAATCCGATATAATTATTAGCTTTGAATCTTG
TCATACAATTGGCAGGATTT

Product: Proline/betaine transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MNNALSKRDFSILIGNAMDHFDTALYGFLAPLLAGIFFPNHDKVVALILTYSVLATSLFTRLIGSYFFGVIAKKYGGVFA
LSYSLIGVALTTSLIGLIPSHAQIGWLAPLLLVLLRTLQGVYSEGECAIAKLFILENKEEKKALKASYLYQTSTMVGIIL
ASFISTIVLNVEYNTYWRLCFIFGGLTALIGYFLRKREDIVVKPSFSSRGLTTGSNKKDKITLRNFWILWSSHGMTPGSS
PRNDVVSSLLQDLTTIWNCKLSILRISFATGFSYMTYIVPFVFLNSFIPLITDISLEIMMKFNTEFLIFDMIMIPIIGHL
TKKLHYLKILNGTLILMSLSIIPLWLCLNNASIWYVNFVRIWIIILGVGFLAPLNCWLNDLFKTADKYMLVGIGSSIGAS
LIGRLTPSICLMLWYVTSNSLSIAVYITAISMVTLWAVRGVVARVNVIPAKAGMTSRTLSNP

Sequences:

>Translated_462_residues
MNNALSKRDFSILIGNAMDHFDTALYGFLAPLLAGIFFPNHDKVVALILTYSVLATSLFTRLIGSYFFGVIAKKYGGVFA
LSYSLIGVALTTSLIGLIPSHAQIGWLAPLLLVLLRTLQGVYSEGECAIAKLFILENKEEKKALKASYLYQTSTMVGIIL
ASFISTIVLNVEYNTYWRLCFIFGGLTALIGYFLRKREDIVVKPSFSSRGLTTGSNKKDKITLRNFWILWSSHGMTPGSS
PRNDVVSSLLQDLTTIWNCKLSILRISFATGFSYMTYIVPFVFLNSFIPLITDISLEIMMKFNTEFLIFDMIMIPIIGHL
TKKLHYLKILNGTLILMSLSIIPLWLCLNNASIWYVNFVRIWIIILGVGFLAPLNCWLNDLFKTADKYMLVGIGSSIGAS
LIGRLTPSICLMLWYVTSNSLSIAVYITAISMVTLWAVRGVVARVNVIPAKAGMTSRTLSNP
>Mature_462_residues
MNNALSKRDFSILIGNAMDHFDTALYGFLAPLLAGIFFPNHDKVVALILTYSVLATSLFTRLIGSYFFGVIAKKYGGVFA
LSYSLIGVALTTSLIGLIPSHAQIGWLAPLLLVLLRTLQGVYSEGECAIAKLFILENKEEKKALKASYLYQTSTMVGIIL
ASFISTIVLNVEYNTYWRLCFIFGGLTALIGYFLRKREDIVVKPSFSSRGLTTGSNKKDKITLRNFWILWSSHGMTPGSS
PRNDVVSSLLQDLTTIWNCKLSILRISFATGFSYMTYIVPFVFLNSFIPLITDISLEIMMKFNTEFLIFDMIMIPIIGHL
TKKLHYLKILNGTLILMSLSIIPLWLCLNNASIWYVNFVRIWIIILGVGFLAPLNCWLNDLFKTADKYMLVGIGSSIGAS
LIGRLTPSICLMLWYVTSNSLSIAVYITAISMVTLWAVRGVVARVNVIPAKAGMTSRTLSNP

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=455, Percent_Identity=25.9340659340659, Blast_Score=101, Evalue=8e-23,
Organism=Escherichia coli, GI1788292, Length=440, Percent_Identity=23.6363636363636, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1789941, Length=200, Percent_Identity=33, Blast_Score=79, Evalue=9e-16,
Organism=Escherichia coli, GI1788942, Length=419, Percent_Identity=22.4343675417661, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 51404; Mature: 51404

Theoretical pI: Translated: 9.89; Mature: 9.89

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNALSKRDFSILIGNAMDHFDTALYGFLAPLLAGIFFPNHDKVVALILTYSVLATSLFT
CCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
RLIGSYFFGVIAKKYGGVFALSYSLIGVALTTSLIGLIPSHAQIGWLAPLLLVLLRTLQG
HHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
VYSEGECAIAKLFILENKEEKKALKASYLYQTSTMVGIILASFISTIVLNVEYNTYWRLC
HCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCHHHHHH
FIFGGLTALIGYFLRKREDIVVKPSFSSRGLTTGSNKKDKITLRNFWILWSSHGMTPGSS
HHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCEEEEEEEEEEEECCCCCCCCC
PRNDVVSSLLQDLTTIWNCKLSILRISFATGFSYMTYIVPFVFLNSFIPLITDISLEIMM
CHHHHHHHHHHHHHHHHHCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEE
KFNTEFLIFDMIMIPIIGHLTKKLHYLKILNGTLILMSLSIIPLWLCLNNASIWYVNFVR
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHH
IWIIILGVGFLAPLNCWLNDLFKTADKYMLVGIGSSIGASLIGRLTPSICLMLWYVTSNS
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCC
LSIAVYITAISMVTLWAVRGVVARVNVIPAKAGMTSRTLSNP
CEEEHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MNNALSKRDFSILIGNAMDHFDTALYGFLAPLLAGIFFPNHDKVVALILTYSVLATSLFT
CCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
RLIGSYFFGVIAKKYGGVFALSYSLIGVALTTSLIGLIPSHAQIGWLAPLLLVLLRTLQG
HHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
VYSEGECAIAKLFILENKEEKKALKASYLYQTSTMVGIILASFISTIVLNVEYNTYWRLC
HCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCHHHHHH
FIFGGLTALIGYFLRKREDIVVKPSFSSRGLTTGSNKKDKITLRNFWILWSSHGMTPGSS
HHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCEEEEEEEEEEEECCCCCCCCC
PRNDVVSSLLQDLTTIWNCKLSILRISFATGFSYMTYIVPFVFLNSFIPLITDISLEIMM
CHHHHHHHHHHHHHHHHHCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEE
KFNTEFLIFDMIMIPIIGHLTKKLHYLKILNGTLILMSLSIIPLWLCLNNASIWYVNFVR
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHH
IWIIILGVGFLAPLNCWLNDLFKTADKYMLVGIGSSIGASLIGRLTPSICLMLWYVTSNS
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCC
LSIAVYITAISMVTLWAVRGVVARVNVIPAKAGMTSRTLSNP
CEEEHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]