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The map label for this gene is rho

Identifier: 229586787

GI number: 229586787

Start: 680480

End: 681856

Strand: Direct

Name: rho

Synonym: RAF_ORF0645

Alternate gene names: 229586787

Gene position: 680480-681856 (Clockwise)

Preceding gene: 229586786

Following gene: 229586788

Centisome position: 53.22

GC content: 36.24

Gene sequence:

>1377_bases
ATGAACACAACTAATAAAGAATCCACAGCAGAACTTAACAATACCGAATCCAATAACAATTATAATAATTTTGCAGAGAA
CGGTAATATTATTAATTTAAAACAATTAAAACGTAAATTACCGGAGGAACTACAAGCTCAAGCAGAAGAATTAAAAATTG
AAAATATTAATTCATTACTTAAACAAGAATTAGTTTTTGCAATTTTGAAAAAATCCGTAGAGCAGGGAGTTTTAATAGTG
GGCGAGGGGGTACTTGAAGTACTACCTGACGGATTTGGTTTTTTACGTTCGCCGGAAGTAAACTACTTAGCAGGCCCTGA
TGATATTTATATTTCTCCTAGTCAGATTCGTCGCTTTGGTCTGCGTACCGGTGATACTGTAGAAGGTCAGATCAGAGCTC
CAAAAGCCGGAGAACGTTATTTTGCTTTACTGAAAGTCAATAGAGTAAATTTTGAAGACCCTTCTAAAGCTTATCATCGT
GTTCATTTTGATAATTTAACCCCCTTATATCCTGATGAAAAATTAGGATTAGAACTTGAAAATAATAGTAAAGATAGTAA
GGATTTTAGTACACGGGTTATTGAACTTGTTGCCCCTATGGGTAAAGGACAACGTGCATTAATAGTAGCACCGCCTCGTA
CAGGTAAAACCGTATTATTACAAAATATAGCACATGCAATTACAACCAATAATCCGGAAGTATTTTTAATAGTACTGTTA
ATAGACGAAAGACCTGAAGAAGTAACCGATATGCAACGTTCCGTGCGTGGGGAGGTTGTTAGCTCTACTTTCGATGAACC
GGCAAGTAGGCACGTGCAGCTTGCAGAAATGGTTATCAAAAAAGCAAAGCGTTTAGTAGAACATAAAAAAGATGTGGTAA
TTTTAGTAGATGCGATAACACGTTTAGCTCGTGCTTATAACACCGTAGTGCCGTCATCAGGTAAAGTATTAACGGGCGGT
GTTGATGCTAATGCTCTTCAGAGACCTAAAAGATTTTTCGGAGCAGCAAGAAATATTGAAAACGGCGGTTCGCTTACTAT
AATCGGTACGGCTTTAATTGAAACCGGTTCTCGTATGGATGAGGTAATTTTTGAGGAGTTTAAAGGTACAGGTAATTCCG
AGATAGTTCTAGATCGTAAGATTGCCGATAAGCGTATTTATCCGGCTATTGATATAACTAGATCAGGAACTAGAAAAGAA
GATTTATTAGTAGATAAAATAATATTAAATAAAATGTGGGTTCTTCGTCGTATTATCGATCCAATGGGTAGCAGTGAAGC
AATAGAATTTTTACTCAAAAAACTTGAAAATACTAAAACTAACGGTGAGTTTTTTGAGATGATGAAGAGTCCTGAACGTC
CTAAGAACGGTCTATAG

Upstream 100 bases:

>100_bases
AAAAATCTAATTTGATTCCTAACTTAAATAAGTATATTACTTACCTTTAAACTTGATTGCTAACTCAACTTTTCAATTAT
AATCTATTAATAATAAAATA

Downstream 100 bases:

>100_bases
TCAATTAATACCTATATTGTGCTTCGTTGTGCGTCGGCCTCACCTTATTATATTATAGGCTTCGCTCCTCACGCCTGTCA
CAGTATAGGGCTGCATTTAG

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MNTTNKESTAELNNTESNNNYNNFAENGNIINLKQLKRKLPEELQAQAEELKIENINSLLKQELVFAILKKSVEQGVLIV
GEGVLEVLPDGFGFLRSPEVNYLAGPDDIYISPSQIRRFGLRTGDTVEGQIRAPKAGERYFALLKVNRVNFEDPSKAYHR
VHFDNLTPLYPDEKLGLELENNSKDSKDFSTRVIELVAPMGKGQRALIVAPPRTGKTVLLQNIAHAITTNNPEVFLIVLL
IDERPEEVTDMQRSVRGEVVSSTFDEPASRHVQLAEMVIKKAKRLVEHKKDVVILVDAITRLARAYNTVVPSSGKVLTGG
VDANALQRPKRFFGAARNIENGGSLTIIGTALIETGSRMDEVIFEEFKGTGNSEIVLDRKIADKRIYPAIDITRSGTRKE
DLLVDKIILNKMWVLRRIIDPMGSSEAIEFLLKKLENTKTNGEFFEMMKSPERPKNGL

Sequences:

>Translated_458_residues
MNTTNKESTAELNNTESNNNYNNFAENGNIINLKQLKRKLPEELQAQAEELKIENINSLLKQELVFAILKKSVEQGVLIV
GEGVLEVLPDGFGFLRSPEVNYLAGPDDIYISPSQIRRFGLRTGDTVEGQIRAPKAGERYFALLKVNRVNFEDPSKAYHR
VHFDNLTPLYPDEKLGLELENNSKDSKDFSTRVIELVAPMGKGQRALIVAPPRTGKTVLLQNIAHAITTNNPEVFLIVLL
IDERPEEVTDMQRSVRGEVVSSTFDEPASRHVQLAEMVIKKAKRLVEHKKDVVILVDAITRLARAYNTVVPSSGKVLTGG
VDANALQRPKRFFGAARNIENGGSLTIIGTALIETGSRMDEVIFEEFKGTGNSEIVLDRKIADKRIYPAIDITRSGTRKE
DLLVDKIILNKMWVLRRIIDPMGSSEAIEFLLKKLENTKTNGEFFEMMKSPERPKNGL
>Mature_458_residues
MNTTNKESTAELNNTESNNNYNNFAENGNIINLKQLKRKLPEELQAQAEELKIENINSLLKQELVFAILKKSVEQGVLIV
GEGVLEVLPDGFGFLRSPEVNYLAGPDDIYISPSQIRRFGLRTGDTVEGQIRAPKAGERYFALLKVNRVNFEDPSKAYHR
VHFDNLTPLYPDEKLGLELENNSKDSKDFSTRVIELVAPMGKGQRALIVAPPRTGKTVLLQNIAHAITTNNPEVFLIVLL
IDERPEEVTDMQRSVRGEVVSSTFDEPASRHVQLAEMVIKKAKRLVEHKKDVVILVDAITRLARAYNTVVPSSGKVLTGG
VDANALQRPKRFFGAARNIENGGSLTIIGTALIETGSRMDEVIFEEFKGTGNSEIVLDRKIADKRIYPAIDITRSGTRKE
DLLVDKIILNKMWVLRRIIDPMGSSEAIEFLLKKLENTKTNGEFFEMMKSPERPKNGL

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family

Homologues:

Organism=Escherichia coli, GI1790217, Length=419, Percent_Identity=66.8257756563246, Blast_Score=580, Evalue=1e-167,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): RHO_RICCN (Q92HL2)

Other databases:

- EMBL:   AE006914
- PIR:   G97794
- RefSeq:   NP_360396.1
- ProteinModelPortal:   Q92HL2
- SMR:   Q92HL2
- GeneID:   927486
- GenomeReviews:   AE006914_GR
- KEGG:   rco:RC0759
- OMA:   YNYLPGP
- ProtClustDB:   PRK09376
- BioCyc:   RCON272944:RC0759-MONOMER
- HAMAP:   MF_01884
- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR011112
- InterPro:   IPR011113
- InterPro:   IPR004665
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00382
- SMART:   SM00357
- SMART:   SM00959
- TIGRFAMs:   TIGR00767

Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind; SSF50249 Nucleic_acid_OB; SSF68912 Rho_N

EC number: NA

Molecular weight: Translated: 51279; Mature: 51279

Theoretical pI: Translated: 7.84; Mature: 7.84

Prosite motif: NA

Important sites: BINDING 244-244

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTTNKESTAELNNTESNNNYNNFAENGNIINLKQLKRKLPEELQAQAEELKIENINSLL
CCCCCCCCHHHCCCCCCCCCCCCHHCCCCEEEHHHHHHHCCHHHHHHHHHHHHHHHHHHH
KQELVFAILKKSVEQGVLIVGEGVLEVLPDGFGFLRSPEVNYLAGPDDIYISPSQIRRFG
HHHHHHHHHHHHHHCCEEEEECCHHHHCCCCCCCCCCCCCCEEECCCCEEECHHHHHHHC
LRTGDTVEGQIRAPKAGERYFALLKVNRVNFEDPSKAYHRVHFDNLTPLYPDEKLGLELE
CCCCCCCCCEEECCCCCCCEEEEEEEECCCCCCHHHHHHEEECCCCCCCCCCHHCCEEEC
NNSKDSKDFSTRVIELVAPMGKGQRALIVAPPRTGKTVLLQNIAHAITTNNPEVFLIVLL
CCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCEEEEEEE
IDERPEEVTDMQRSVRGEVVSSTFDEPASRHVQLAEMVIKKAKRLVEHKKDVVILVDAIT
ECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHH
RLARAYNTVVPSSGKVLTGGVDANALQRPKRFFGAARNIENGGSLTIIGTALIETGSRMD
HHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHCCCHHH
EVIFEEFKGTGNSEIVLDRKIADKRIYPAIDITRSGTRKEDLLVDKIILNKMWVLRRIID
HHHHHHHCCCCCCEEEEECCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHC
PMGSSEAIEFLLKKLENTKTNGEFFEMMKSPERPKNGL
CCCCHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MNTTNKESTAELNNTESNNNYNNFAENGNIINLKQLKRKLPEELQAQAEELKIENINSLL
CCCCCCCCHHHCCCCCCCCCCCCHHCCCCEEEHHHHHHHCCHHHHHHHHHHHHHHHHHHH
KQELVFAILKKSVEQGVLIVGEGVLEVLPDGFGFLRSPEVNYLAGPDDIYISPSQIRRFG
HHHHHHHHHHHHHHCCEEEEECCHHHHCCCCCCCCCCCCCCEEECCCCEEECHHHHHHHC
LRTGDTVEGQIRAPKAGERYFALLKVNRVNFEDPSKAYHRVHFDNLTPLYPDEKLGLELE
CCCCCCCCCEEECCCCCCCEEEEEEEECCCCCCHHHHHHEEECCCCCCCCCCHHCCEEEC
NNSKDSKDFSTRVIELVAPMGKGQRALIVAPPRTGKTVLLQNIAHAITTNNPEVFLIVLL
CCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCEEEEEEE
IDERPEEVTDMQRSVRGEVVSSTFDEPASRHVQLAEMVIKKAKRLVEHKKDVVILVDAIT
ECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHH
RLARAYNTVVPSSGKVLTGGVDANALQRPKRFFGAARNIENGGSLTIIGTALIETGSRMD
HHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHCCCHHH
EVIFEEFKGTGNSEIVLDRKIADKRIYPAIDITRSGTRKEDLLVDKIILNKMWVLRRIID
HHHHHHHCCCCCCEEEEECCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHC
PMGSSEAIEFLLKKLENTKTNGEFFEMMKSPERPKNGL
CCCCHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893