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The map label for this gene is folC [H]

Identifier: 229586772

GI number: 229586772

Start: 664996

End: 666276

Strand: Direct

Name: folC [H]

Synonym: RAF_ORF0626

Alternate gene names: 229586772

Gene position: 664996-666276 (Clockwise)

Preceding gene: 229586771

Following gene: 229586773

Centisome position: 52.01

GC content: 34.58

Gene sequence:

>1281_bases
ATGCTAATGCCGCATTTTCCGGTGCCGACCTGGAAAAATAACATTAAATATGATCTTGAAAATATAGCAAGACTTTTAAG
AGCCTTAGGTAACTCTCATTTAAGGCTTCCTCCCGTCATACATATAGCAGGTACAAACGGTAAAGGTTCAAGTAGTGCTA
TGCTTAAAAGCATCTTTACGCTTGCCGGTTATAAGGTACATTGCTATACATCTCCTCATTTATTAGAATTTAATGAACGA
ATCGTAGTAGCAGGTGAGCAAATCTCGGATAATGAATTATGGCAGGTTTGTGAGCAGGTGAGGGTAGCAAGCGAAAAATT
TAATATTGAACCTAGCTTCTTTGAAGGTACTACGGCAGCAGCGTTTCTAGCATTTGAGAAAACAAAAGCCGATATATTAA
TTTTAGAGACGGGACTAGGCGGTCGGTTAGACGCAACAAATATAATAGAACAACCGTTAATTACTTTAATTACACCTATT
TCATATGATCATATGCATATGCTGGGTAATACATTACCCCTAATAGCAATTGAGAAAGCTGGTATTATGAAGTCTTGCGT
TCCTTGCGTTATAAGTATGCAAGTTTTGGAAGTTTATGAAACATTATTTGCCAAGGCTGAAGAGTTAGAAGTACCGAGTT
TCTGTTATGAATATGATTTCGGTATTAAAAAGACGGACAACGGATTTATTTATTCATCACAAAATTTTACCTATGAGTTT
CCTACTCCTTCGCTACTTGGTGATCATCAATTAATAAATGCTGCAAGCGTTATTGCCTTAATAAGCCTTATTAACAAACA
ATTTAATATTAGTAATGAGGTTATTGCTAAAGGATTACAAAATACTATTTGGCCTGCTAGAATCGAAAAAATAGAACCGA
AAAAATATTCTAAATTAATATGTGATAATGTTCAAATTTGGATAGACGTAGCTCACAATAATAGCGGAGCTCAAGTTTTG
GCAAATTGGATGCGTGATAATTTAAAATCATCGATATATTTAATACTCGGTATGACTAAAAATAGGAATATTGAGGAATT
TTGCTCTTACTTTAAGGATTTGACAATTAAAGGTTACGGCGTTAAAGTTTTATCCGAACCGCTAAGCCATAGTGCAGAAA
CAATACATTTGGAAGGCAGAAAGAGTGGTATTGATTTTAGCGCAAGCGACTCACTCGAAGAAGCAATTAGTGATATAAAG
AAGATAAACGGCGATAATAAAGCAAATATTATAATAACCGGATCGCTTTATTTAGCTTCCGATTTTTATAAGTTACTATG
A

Upstream 100 bases:

>100_bases
TTAAAAGTAACGGATTCCCGCTTGCGCGGGAATGATATCGATCTTTAAGTTGACAACAAATATGATATAACTCTATCATT
CATGAAAAAAATAAATAACA

Downstream 100 bases:

>100_bases
TGTCATGCCTGCGTGGATACTAAATCGTCATTGCAAGCAACTGCAAGGAGCGTGGCAATCTCATAAAGTAGGACTCCTGA
GATTGCTTCGTCAAAATTTT

Product: Folylpolyglutamate synthase

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MLMPHFPVPTWKNNIKYDLENIARLLRALGNSHLRLPPVIHIAGTNGKGSSSAMLKSIFTLAGYKVHCYTSPHLLEFNER
IVVAGEQISDNELWQVCEQVRVASEKFNIEPSFFEGTTAAAFLAFEKTKADILILETGLGGRLDATNIIEQPLITLITPI
SYDHMHMLGNTLPLIAIEKAGIMKSCVPCVISMQVLEVYETLFAKAEELEVPSFCYEYDFGIKKTDNGFIYSSQNFTYEF
PTPSLLGDHQLINAASVIALISLINKQFNISNEVIAKGLQNTIWPARIEKIEPKKYSKLICDNVQIWIDVAHNNSGAQVL
ANWMRDNLKSSIYLILGMTKNRNIEEFCSYFKDLTIKGYGVKVLSEPLSHSAETIHLEGRKSGIDFSASDSLEEAISDIK
KINGDNKANIIITGSLYLASDFYKLL

Sequences:

>Translated_426_residues
MLMPHFPVPTWKNNIKYDLENIARLLRALGNSHLRLPPVIHIAGTNGKGSSSAMLKSIFTLAGYKVHCYTSPHLLEFNER
IVVAGEQISDNELWQVCEQVRVASEKFNIEPSFFEGTTAAAFLAFEKTKADILILETGLGGRLDATNIIEQPLITLITPI
SYDHMHMLGNTLPLIAIEKAGIMKSCVPCVISMQVLEVYETLFAKAEELEVPSFCYEYDFGIKKTDNGFIYSSQNFTYEF
PTPSLLGDHQLINAASVIALISLINKQFNISNEVIAKGLQNTIWPARIEKIEPKKYSKLICDNVQIWIDVAHNNSGAQVL
ANWMRDNLKSSIYLILGMTKNRNIEEFCSYFKDLTIKGYGVKVLSEPLSHSAETIHLEGRKSGIDFSASDSLEEAISDIK
KINGDNKANIIITGSLYLASDFYKLL
>Mature_426_residues
MLMPHFPVPTWKNNIKYDLENIARLLRALGNSHLRLPPVIHIAGTNGKGSSSAMLKSIFTLAGYKVHCYTSPHLLEFNER
IVVAGEQISDNELWQVCEQVRVASEKFNIEPSFFEGTTAAAFLAFEKTKADILILETGLGGRLDATNIIEQPLITLITPI
SYDHMHMLGNTLPLIAIEKAGIMKSCVPCVISMQVLEVYETLFAKAEELEVPSFCYEYDFGIKKTDNGFIYSSQNFTYEF
PTPSLLGDHQLINAASVIALISLINKQFNISNEVIAKGLQNTIWPARIEKIEPKKYSKLICDNVQIWIDVAHNNSGAQVL
ANWMRDNLKSSIYLILGMTKNRNIEEFCSYFKDLTIKGYGVKVLSEPLSHSAETIHLEGRKSGIDFSASDSLEEAISDIK
KINGDNKANIIITGSLYLASDFYKLL

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=320, Percent_Identity=26.875, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI66932990, Length=321, Percent_Identity=26.791277258567, Blast_Score=120, Evalue=3e-27,
Organism=Escherichia coli, GI1788654, Length=393, Percent_Identity=27.4809160305344, Blast_Score=143, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17553150, Length=344, Percent_Identity=29.3604651162791, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI71984923, Length=344, Percent_Identity=29.3604651162791, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17553148, Length=344, Percent_Identity=29.3604651162791, Blast_Score=137, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6323760, Length=429, Percent_Identity=25.8741258741259, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6324815, Length=433, Percent_Identity=28.6374133949192, Blast_Score=124, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6322718, Length=297, Percent_Identity=24.5791245791246, Blast_Score=67, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24641571, Length=306, Percent_Identity=28.7581699346405, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24581568, Length=192, Percent_Identity=33.3333333333333, Blast_Score=99, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 47607; Mature: 47607

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLMPHFPVPTWKNNIKYDLENIARLLRALGNSHLRLPPVIHIAGTNGKGSSSAMLKSIFT
CCCCCCCCCCCCCCCEECHHHHHHHHHHHCCCCEECCCEEEEECCCCCCCHHHHHHHHHH
LAGYKVHCYTSPHLLEFNERIVVAGEQISDNELWQVCEQVRVASEKFNIEPSFFEGTTAA
HCCCEEEEECCCCEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHH
AFLAFEKTKADILILETGLGGRLDATNIIEQPLITLITPISYDHMHMLGNTLPLIAIEKA
EEEEEECCCCCEEEEECCCCCCCCHHHHHHHCHHHHCCCCCCHHHHHHCCCCCEEEEECC
GIMKSCVPCVISMQVLEVYETLFAKAEELEVPSFCYEYDFGIKKTDNGFIYSSQNFTYEF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHEEECCCCEECCCCEEEECCCEEEEC
PTPSLLGDHQLINAASVIALISLINKQFNISNEVIAKGLQNTIWPARIEKIEPKKYSKLI
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHCCCCHHHHHHH
CDNVQIWIDVAHNNSGAQVLANWMRDNLKSSIYLILGMTKNRNIEEFCSYFKDLTIKGYG
HCCEEEEEEEEECCCCHHHHHHHHHHHHCCEEEEEEEECCCCCHHHHHHHHHCCEECCCC
VKVLSEPLSHSAETIHLEGRKSGIDFSASDSLEEAISDIKKINGDNKANIIITGSLYLAS
HHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECHHHHH
DFYKLL
HHHHHC
>Mature Secondary Structure
MLMPHFPVPTWKNNIKYDLENIARLLRALGNSHLRLPPVIHIAGTNGKGSSSAMLKSIFT
CCCCCCCCCCCCCCCEECHHHHHHHHHHHCCCCEECCCEEEEECCCCCCCHHHHHHHHHH
LAGYKVHCYTSPHLLEFNERIVVAGEQISDNELWQVCEQVRVASEKFNIEPSFFEGTTAA
HCCCEEEEECCCCEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHH
AFLAFEKTKADILILETGLGGRLDATNIIEQPLITLITPISYDHMHMLGNTLPLIAIEKA
EEEEEECCCCCEEEEECCCCCCCCHHHHHHHCHHHHCCCCCCHHHHHHCCCCCEEEEECC
GIMKSCVPCVISMQVLEVYETLFAKAEELEVPSFCYEYDFGIKKTDNGFIYSSQNFTYEF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHEEECCCCEECCCCEEEECCCEEEEC
PTPSLLGDHQLINAASVIALISLINKQFNISNEVIAKGLQNTIWPARIEKIEPKKYSKLI
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHCCCCHHHHHHH
CDNVQIWIDVAHNNSGAQVLANWMRDNLKSSIYLILGMTKNRNIEEFCSYFKDLTIKGYG
HCCEEEEEEEEECCCCHHHHHHHHHHHHCCEEEEEEEECCCCCHHHHHHHHHCCEECCCC
VKVLSEPLSHSAETIHLEGRKSGIDFSASDSLEEAISDIKKINGDNKANIIITGSLYLAS
HHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECHHHHH
DFYKLL
HHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]