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The map label for this gene is def2 [C]

Identifier: 229586763

GI number: 229586763

Start: 654658

End: 655209

Strand: Direct

Name: def2 [C]

Synonym: RAF_ORF0617

Alternate gene names: 229586763

Gene position: 654658-655209 (Clockwise)

Preceding gene: 229586762

Following gene: 229586764

Centisome position: 51.2

GC content: 31.88

Gene sequence:

>552_bases
ATGAATCAAGATAAACCTTACTATCAAATAGTTTATGCACCAAACGATATTTTTAAAAAACAAGCAGAATATATAGACAT
TGTTGATGATAATATTCGTACTATTGTCGATAAAATGCTTCAGAATTTACATATAGAAAGGGCAGTGGGCTTGGGTGCTA
ATATGGTAGGAATATTAAAGCGTATAGCGGTAGTTGACCTACATGAAAATAATAAATCATCGCCTATAGTTTTTATTAAT
CCGAACATAACTTATTTTTCAGAAGAAAAACAAACTTTTATTGAGGGTTCTTTATCGTTTCCAGGCATTGAAGCATCTAT
TACACGATCAAAAGCAATCAAGGTAAAATATCTTGATTATAACGGTAATAAGCAAGAACTAGCAGCAGAAGGCTTTTTAG
CAACTGTAATTCAGCATGAAATAGATTATTTAAACGGCAAAACTTTCTTAGATTATTTATCAAAACTTAAGCGTGATACA
CTACTTAAAAAGATGCTAAAGCATATCAAACTCCATCCTCCACATATTCACGGTAGCGGTTGTAGGCATTAG

Upstream 100 bases:

>100_bases
GACGGGAAAACCGGTATACGCAACAATGCTCGCTCGCAATAACGATATCTATCTTTATTTAGCAATGCAAAAATAACTCT
TCATTTATCGGAGTATATTA

Downstream 100 bases:

>100_bases
ACATTTTCTCAAACTTCGCTTCCAGAGGTAATTTATACATCAAGCCTAGACTCGTATCCTCACGTACTAGTTTGTACGCT
GCGGTGACAAGTGCTTTCGT

Product: Polypeptide deformylase

Products: Formate; L-Methionine. [C]

Alternate protein names: Polypeptide deformylase-like [H]

Number of amino acids: Translated: 183; Mature: 183

Protein sequence:

>183_residues
MNQDKPYYQIVYAPNDIFKKQAEYIDIVDDNIRTIVDKMLQNLHIERAVGLGANMVGILKRIAVVDLHENNKSSPIVFIN
PNITYFSEEKQTFIEGSLSFPGIEASITRSKAIKVKYLDYNGNKQELAAEGFLATVIQHEIDYLNGKTFLDYLSKLKRDT
LLKKMLKHIKLHPPHIHGSGCRH

Sequences:

>Translated_183_residues
MNQDKPYYQIVYAPNDIFKKQAEYIDIVDDNIRTIVDKMLQNLHIERAVGLGANMVGILKRIAVVDLHENNKSSPIVFIN
PNITYFSEEKQTFIEGSLSFPGIEASITRSKAIKVKYLDYNGNKQELAAEGFLATVIQHEIDYLNGKTFLDYLSKLKRDT
LLKKMLKHIKLHPPHIHGSGCRH
>Mature_183_residues
MNQDKPYYQIVYAPNDIFKKQAEYIDIVDDNIRTIVDKMLQNLHIERAVGLGANMVGILKRIAVVDLHENNKSSPIVFIN
PNITYFSEEKQTFIEGSLSFPGIEASITRSKAIKVKYLDYNGNKQELAAEGFLATVIQHEIDYLNGKTFLDYLSKLKRDT
LLKKMLKHIKLHPPHIHGSGCRH

Specific function: Removes The Formyl Group From The N-Terminal Met Of Newly Synthesized Proteins. Requires At Least A Dipeptide For An Efficient Rate Of Reaction. N-Terminal L-Methionine Is A Prerequisite For Activity But The Enzyme Has Broad Specificity At Other Positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=168, Percent_Identity=25, Blast_Score=76, Evalue=1e-14,
Organism=Escherichia coli, GI1789682, Length=159, Percent_Identity=38.9937106918239, Blast_Score=114, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24645726, Length=168, Percent_Identity=27.9761904761905, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24645728, Length=166, Percent_Identity=28.3132530120482, Blast_Score=75, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: 3.5.1.88 [C]

Molecular weight: Translated: 20941; Mature: 20941

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQDKPYYQIVYAPNDIFKKQAEYIDIVDDNIRTIVDKMLQNLHIERAVGLGANMVGILK
CCCCCCEEEEEECCHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
RIAVVDLHENNKSSPIVFINPNITYFSEEKQTFIEGSLSFPGIEASITRSKAIKVKYLDY
HHHHEEECCCCCCCCEEEECCCEEEECCCHHHHHCCCCCCCCCCCEEECCEEEEEEEEEC
NGNKQELAAEGFLATVIQHEIDYLNGKTFLDYLSKLKRDTLLKKMLKHIKLHPPHIHGSG
CCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
CRH
CCC
>Mature Secondary Structure
MNQDKPYYQIVYAPNDIFKKQAEYIDIVDDNIRTIVDKMLQNLHIERAVGLGANMVGILK
CCCCCCEEEEEECCHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
RIAVVDLHENNKSSPIVFINPNITYFSEEKQTFIEGSLSFPGIEASITRSKAIKVKYLDY
HHHHEEECCCCCCCCEEEECCCEEEECCCHHHHHCCCCCCCCCCCEEECCEEEEEEEEEC
NGNKQELAAEGFLATVIQHEIDYLNGKTFLDYLSKLKRDTLLKKMLKHIKLHPPHIHGSG
CCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
CRH
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-Formyl-L-Methionine; H2O [C]

Specific reaction: N-Formyl-L-Methionine + H2O = Formate + L-Methionine. [C]

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: Dithiothreitol; EDTA; o-Phenantroline [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]