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The map label for this gene is murD [H]

Identifier: 229586685

GI number: 229586685

Start: 552783

End: 554285

Strand: Direct

Name: murD [H]

Synonym: RAF_ORF0522

Alternate gene names: 229586685

Gene position: 552783-554285 (Clockwise)

Preceding gene: 229586684

Following gene: 229586686

Centisome position: 43.24

GC content: 33.2

Gene sequence:

>1503_bases
ATGAATTCTCATACAAAACAAAAAATAGGTGTTTTTGGGCTTGGTAAAACCGGTATATCGGTTTACGAGGAGTTGCAAAA
TAAATATGATGTAATTGTTTATGATGATTTAAAAGCAAATAGAGATATATTTGAAGAATTATATAGTAAAACTGCTATCG
CTGCTTTATCTGATTCAAGATGGCAAAATTTAGATACAATTGTCTTAAGTCCTGGAATTCCTTTAACGCATGAGATAGTC
AATATTGCAAAAAGTTTTAGTATTCCAATCACTTCCGATATAGACTTATTATTTGCAAAATCAAAAAACTTAAAATTTAT
AGCTATAACCGGTACAAACGGTAAAAGTACTACTACTGCTTTAATAAGCCATATCTTAAATAGTAGCGGTTTAGACTATC
CGGTTGCCGGTAATATTGGAGTTTCTGCTTTGCAAGCTAAAGCAAGTAAGGATGGATATGTACTTGAATTGTCTTCTTTT
CAGCTAGATCTAGTAAAAACCTTTACAGCTAAAATAGCCGTGCTTCTTAATATAACTCCCGACCATTTAGATAGACATCA
GGATATGACCTGCTATATTGCCGCAAAATCTAAGATCTTTGATCGCATGGATCAAGAGAGTTATGCGGTAATTAATATCG
ATAATGATTATTGTCGTGAAGTCTTTATAAAATTGCAGCAAGAGCAGCGTATAAAGCTAATTCCTTTTTCAGTTGCTCAA
ATTCTTGAAAACGGTATTTCGGTAGTTGATGATAAAATTAGCGCTAATTTTTTTGATGATATAAGTTTTGAATTACAGCA
TAATTCAGAAAGTTTGAGACAATATGAATTTCAAGGTGAGCCTGTGGAGCCGGAGTGTATAAAAATACGTGAGCATAGGC
AAGACCTGCAAAATTCGCTTGTATCAAGCTTTATGCATTATGCTGTACCTTTTAATAAAAACCTGCAAGGAGTTCATAAT
TGTGAGAATATTGCAGCAAGTTATGCAGTAGCTAAAATAATAGGAGTAGAACCTAAAAAAATACTTGAATCTATAAGTAG
TTTTCAAAGTTTACCTCATAGAATGCAATATATCGGTAGTATAAATAATATAAGTTTTTACAATGATAGTAAAGCGACAA
ATGCCATATCTGCCGTGCAATCAATTAAAGCACTCGATAATATTTACTGGCTTGCAGGAGGAATTCCTAAAGAGGGTGGT
ATTGAGGAAATAAAGCCTTATTTCAGTCACATTAAGAAAGCTTATTTTTATGGTCAAGCTAAAGAAATATTTGCAAATAC
TGCTAAGAATATAGTAGATTTTATAATATGTGATAATCTTGAGCAGGCTTTCGATCTTGCTTATAAGGATGCAGTAGGTG
ATAACGCGGAGGTAAAAAATATCTTGTTAGCACCTAGTTGTAGCTCATATGATCAGTTTAAAAATTTTGAAGAGCGTGGC
GAGTTGTTTATAAAATTGTGTAGTATCCTGTCACTCCGTGGCTTGACCACGGGGTCTAGTTAA

Upstream 100 bases:

>100_bases
TGCTCCGCTCCTCGACTTGTAAACTCCTTGCTCTTTTTGAAGTTGATCAAGTATACCTGTTCTTGAATACTAAGACTGCA
ATGTTTTTTCAAATTAATTC

Downstream 100 bases:

>100_bases
AAATACTAAATAATATTAGTATTTTTTATTGTTTTTCTGGATACCGTGGTCAAGCCACGGAGTGACATCGAGGGTGTTTT
CCGATCCATGCAATAACATT

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 500; Mature: 500

Protein sequence:

>500_residues
MNSHTKQKIGVFGLGKTGISVYEELQNKYDVIVYDDLKANRDIFEELYSKTAIAALSDSRWQNLDTIVLSPGIPLTHEIV
NIAKSFSIPITSDIDLLFAKSKNLKFIAITGTNGKSTTTALISHILNSSGLDYPVAGNIGVSALQAKASKDGYVLELSSF
QLDLVKTFTAKIAVLLNITPDHLDRHQDMTCYIAAKSKIFDRMDQESYAVINIDNDYCREVFIKLQQEQRIKLIPFSVAQ
ILENGISVVDDKISANFFDDISFELQHNSESLRQYEFQGEPVEPECIKIREHRQDLQNSLVSSFMHYAVPFNKNLQGVHN
CENIAASYAVAKIIGVEPKKILESISSFQSLPHRMQYIGSINNISFYNDSKATNAISAVQSIKALDNIYWLAGGIPKEGG
IEEIKPYFSHIKKAYFYGQAKEIFANTAKNIVDFIICDNLEQAFDLAYKDAVGDNAEVKNILLAPSCSSYDQFKNFEERG
ELFIKLCSILSLRGLTTGSS

Sequences:

>Translated_500_residues
MNSHTKQKIGVFGLGKTGISVYEELQNKYDVIVYDDLKANRDIFEELYSKTAIAALSDSRWQNLDTIVLSPGIPLTHEIV
NIAKSFSIPITSDIDLLFAKSKNLKFIAITGTNGKSTTTALISHILNSSGLDYPVAGNIGVSALQAKASKDGYVLELSSF
QLDLVKTFTAKIAVLLNITPDHLDRHQDMTCYIAAKSKIFDRMDQESYAVINIDNDYCREVFIKLQQEQRIKLIPFSVAQ
ILENGISVVDDKISANFFDDISFELQHNSESLRQYEFQGEPVEPECIKIREHRQDLQNSLVSSFMHYAVPFNKNLQGVHN
CENIAASYAVAKIIGVEPKKILESISSFQSLPHRMQYIGSINNISFYNDSKATNAISAVQSIKALDNIYWLAGGIPKEGG
IEEIKPYFSHIKKAYFYGQAKEIFANTAKNIVDFIICDNLEQAFDLAYKDAVGDNAEVKNILLAPSCSSYDQFKNFEERG
ELFIKLCSILSLRGLTTGSS
>Mature_500_residues
MNSHTKQKIGVFGLGKTGISVYEELQNKYDVIVYDDLKANRDIFEELYSKTAIAALSDSRWQNLDTIVLSPGIPLTHEIV
NIAKSFSIPITSDIDLLFAKSKNLKFIAITGTNGKSTTTALISHILNSSGLDYPVAGNIGVSALQAKASKDGYVLELSSF
QLDLVKTFTAKIAVLLNITPDHLDRHQDMTCYIAAKSKIFDRMDQESYAVINIDNDYCREVFIKLQQEQRIKLIPFSVAQ
ILENGISVVDDKISANFFDDISFELQHNSESLRQYEFQGEPVEPECIKIREHRQDLQNSLVSSFMHYAVPFNKNLQGVHN
CENIAASYAVAKIIGVEPKKILESISSFQSLPHRMQYIGSINNISFYNDSKATNAISAVQSIKALDNIYWLAGGIPKEGG
IEEIKPYFSHIKKAYFYGQAKEIFANTAKNIVDFIICDNLEQAFDLAYKDAVGDNAEVKNILLAPSCSSYDQFKNFEERG
ELFIKLCSILSLRGLTTGSS

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RPE3 insert domain [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=500, Percent_Identity=28.4, Blast_Score=154, Evalue=9e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR022437
- InterPro:   IPR005762 [H]

Pfam domain/function: PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 55835; Mature: 55835

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS00012 PHOSPHOPANTETHEINE ; PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSHTKQKIGVFGLGKTGISVYEELQNKYDVIVYDDLKANRDIFEELYSKTAIAALSDSR
CCCCCCCCCCEEECCCHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCC
WQNLDTIVLSPGIPLTHEIVNIAKSFSIPITSDIDLLFAKSKNLKFIAITGTNGKSTTTA
CCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEEEEECCCCCHHHHH
LISHILNSSGLDYPVAGNIGVSALQAKASKDGYVLELSSFQLDLVKTFTAKIAVLLNITP
HHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHEEEEEECCH
DHLDRHQDMTCYIAAKSKIFDRMDQESYAVINIDNDYCREVFIKLQQEQRIKLIPFSVAQ
HHHCCCCCCEEEEEEHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHCCCCEEEECHHHHH
ILENGISVVDDKISANFFDDISFELQHNSESLRQYEFQGEPVEPECIKIREHRQDLQNSL
HHHCCHHHHCCCCCCCHHCCCEEEEECCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
VSSFMHYAVPFNKNLQGVHNCENIAASYAVAKIIGVEPKKILESISSFQSLPHRMQYIGS
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC
INNISFYNDSKATNAISAVQSIKALDNIYWLAGGIPKEGGIEEIKPYFSHIKKAYFYGQA
CCCEEEECCCHHHHHHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHHHHHHHCHH
KEIFANTAKNIVDFIICDNLEQAFDLAYKDAVGDNAEVKNILLAPSCSSYDQFKNFEERG
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHCCHHHHH
ELFIKLCSILSLRGLTTGSS
HHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MNSHTKQKIGVFGLGKTGISVYEELQNKYDVIVYDDLKANRDIFEELYSKTAIAALSDSR
CCCCCCCCCCEEECCCHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCC
WQNLDTIVLSPGIPLTHEIVNIAKSFSIPITSDIDLLFAKSKNLKFIAITGTNGKSTTTA
CCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEEEEECCCCCHHHHH
LISHILNSSGLDYPVAGNIGVSALQAKASKDGYVLELSSFQLDLVKTFTAKIAVLLNITP
HHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHEEEEEECCH
DHLDRHQDMTCYIAAKSKIFDRMDQESYAVINIDNDYCREVFIKLQQEQRIKLIPFSVAQ
HHHCCCCCCEEEEEEHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHCCCCEEEECHHHHH
ILENGISVVDDKISANFFDDISFELQHNSESLRQYEFQGEPVEPECIKIREHRQDLQNSL
HHHCCHHHHCCCCCCCHHCCCEEEEECCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
VSSFMHYAVPFNKNLQGVHNCENIAASYAVAKIIGVEPKKILESISSFQSLPHRMQYIGS
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC
INNISFYNDSKATNAISAVQSIKALDNIYWLAGGIPKEGGIEEIKPYFSHIKKAYFYGQA
CCCEEEECCCHHHHHHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHHHHHHHCHH
KEIFANTAKNIVDFIICDNLEQAFDLAYKDAVGDNAEVKNILLAPSCSSYDQFKNFEERG
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHCCHHHHH
ELFIKLCSILSLRGLTTGSS
HHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]