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The map label for this gene is 229586668

Identifier: 229586668

GI number: 229586668

Start: 534531

End: 535157

Strand: Direct

Name: 229586668

Synonym: RAF_ORF0505

Alternate gene names: NA

Gene position: 534531-535157 (Clockwise)

Preceding gene: 229586661

Following gene: 229586669

Centisome position: 41.81

GC content: 30.94

Gene sequence:

>627_bases
GTGAAAAAATTTATATTTTGTTTTTTATGTTTATGGACATTAAACATATTTGCTGCTTCTAAAACTTACCCTAATAAGCT
TAATCGTTGTAAAATTACTAGAAATATATTTAATGATTATGAACCGAAAGTTTTTGAAACTACTAATAATTTATTACGTA
AAACAGGAAGATTATCAAAATTTTACGGTGAAAGGATATTAATTAAAGGAAAAGTATTAGATCAAAATTGCGTGCCGGTT
GCAGATGCTAAAGTGTATTTATGGCAAGCAGGAAGCGGGGGTAAATATCCTTACGAACCTTTAAAAACTAGAGTTGATAA
AAGAAGATTTACAAGTAAAAGCGATTCAAGTTTTACCGGTTCCGGTATTGCTACTACTAATAATAAAGGAGAATATTACT
TTATTAGTATGTTGCCTTATACATCTTCTCGTTATTTGAGAAGTGCAAATATAAGAATAGAGCATCCTAGTCTTACTACT
CTCGAAACACGCTTAGACTTATCTGATCAAAATATGTGTGATAATGAGTGCGGTGAAGTAAATCCGATATTGATCGAACC
GCAAGAAAATATGCTGTCTTATTGTTTTGACTTAGTTTTGCAAGGTATGACGCTTAAGAGGTATTGA

Upstream 100 bases:

>100_bases
ATCAATTACTTAATAATCAAATGAGAATAAAAATAATTTGATTAATAAAAATAATTGATTTAAATTTAGATGCAGAATTT
TATAAATATAGCGGTGCAAA

Downstream 100 bases:

>100_bases
TACAGTACGTCATTGCGAGGAAAATGACGAAGTAATTTGACGAAGCAATCTCAGGATATTTGACGAGATTGCCACGCAGT
CTACGACTGCTCGCAATGAC

Product: Protocatechuate-3,4-dioxygenase, beta subunit

Products: 3-carboxy-cis,cis-muconate

Alternate protein names: NA

Number of amino acids: Translated: 208; Mature: 208

Protein sequence:

>208_residues
MKKFIFCFLCLWTLNIFAASKTYPNKLNRCKITRNIFNDYEPKVFETTNNLLRKTGRLSKFYGERILIKGKVLDQNCVPV
ADAKVYLWQAGSGGKYPYEPLKTRVDKRRFTSKSDSSFTGSGIATTNNKGEYYFISMLPYTSSRYLRSANIRIEHPSLTT
LETRLDLSDQNMCDNECGEVNPILIEPQENMLSYCFDLVLQGMTLKRY

Sequences:

>Translated_208_residues
MKKFIFCFLCLWTLNIFAASKTYPNKLNRCKITRNIFNDYEPKVFETTNNLLRKTGRLSKFYGERILIKGKVLDQNCVPV
ADAKVYLWQAGSGGKYPYEPLKTRVDKRRFTSKSDSSFTGSGIATTNNKGEYYFISMLPYTSSRYLRSANIRIEHPSLTT
LETRLDLSDQNMCDNECGEVNPILIEPQENMLSYCFDLVLQGMTLKRY
>Mature_208_residues
MKKFIFCFLCLWTLNIFAASKTYPNKLNRCKITRNIFNDYEPKVFETTNNLLRKTGRLSKFYGERILIKGKVLDQNCVPV
ADAKVYLWQAGSGGKYPYEPLKTRVDKRRFTSKSDSSFTGSGIATTNNKGEYYFISMLPYTSSRYLRSANIRIEHPSLTT
LETRLDLSDQNMCDNECGEVNPILIEPQENMLSYCFDLVLQGMTLKRY

Specific function: Unknown

COG id: COG3485

COG function: function code Q; Protocatechuate 3,4-dioxygenase beta subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the intradiol ring-cleavage dioxygenase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000627
- InterPro:   IPR015889 [H]

Pfam domain/function: PF00775 Dioxygenase_C [H]

EC number: 1.13.11.3

Molecular weight: Translated: 24064; Mature: 24064

Theoretical pI: Translated: 9.44; Mature: 9.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKFIFCFLCLWTLNIFAASKTYPNKLNRCKITRNIFNDYEPKVFETTNNLLRKTGRLSK
CCHHHHHHHHHHHHHHHHCCCCCCCCHHHHEEEHHHHCCCCCCEEHHHHHHHHHHHHHHH
FYGERILIKGKVLDQNCVPVADAKVYLWQAGSGGKYPYEPLKTRVDKRRFTSKSDSSFTG
HCCCEEEEEEEEECCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCC
SGIATTNNKGEYYFISMLPYTSSRYLRSANIRIEHPSLTTLETRLDLSDQNMCDNECGEV
CCEEEECCCCCEEEEEECCCCCCCCEEECCEEEECCCCEEEEEEECCCCCCCCCCCCCCC
NPILIEPQENMLSYCFDLVLQGMTLKRY
CCEEECCHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKKFIFCFLCLWTLNIFAASKTYPNKLNRCKITRNIFNDYEPKVFETTNNLLRKTGRLSK
CCHHHHHHHHHHHHHHHHCCCCCCCCHHHHEEEHHHHCCCCCCEEHHHHHHHHHHHHHHH
FYGERILIKGKVLDQNCVPVADAKVYLWQAGSGGKYPYEPLKTRVDKRRFTSKSDSSFTG
HCCCEEEEEEEEECCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCC
SGIATTNNKGEYYFISMLPYTSSRYLRSANIRIEHPSLTTLETRLDLSDQNMCDNECGEV
CCEEEECCCCCEEEEEECCCCCCCCEEECCEEEECCCCEEEEEEECCCCCCCCCCCCCCC
NPILIEPQENMLSYCFDLVLQGMTLKRY
CCEEECCHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3,4-dihydroxybenzoate; O2

Specific reaction: 3,4-dihydroxybenzoate + O2 = 3-carboxy-cis,cis-muconate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]