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The map label for this gene is rfbD [C]

Identifier: 229586599

GI number: 229586599

Start: 451648

End: 452502

Strand: Direct

Name: rfbD [C]

Synonym: RAF_ORF0425

Alternate gene names: 229586599

Gene position: 451648-452502 (Clockwise)

Preceding gene: 229586598

Following gene: 229586600

Centisome position: 35.33

GC content: 34.74

Gene sequence:

>855_bases
ATGAAAATATTAATTTTAGGTGTTACCGGTATGCTGGGTAATAGCATATTCAGGTTTTTAAGTAGTGATAAAAAATTTGA
CATTTTTGCGACAGCCCGAAATAATTCAGCTCGTTCTTACTTTTCTAAGGATTTATCCGATAAATTAATTACAAATGTAG
ATGTTGAGAACCATGATGCGTTAGTTGAGGTATTTAATCAAACAAAACCCGATATAGTAATAAATTGTATAGGGCTTGTA
AAACAATTGGCAGATGCTAATGATCCGTTAAAAGCATTACCGATAAATAGTTTGCTGCCTCATAGGCTAGCTAATTTATG
TAAACTAGCTAATTCAAGGTTAATCCATATTAGTACTGATTGTGTGTTTTCAGGAAAAAAAGGTAATTATAAGGAAAGCG
ATTTTTCTGACTGTTATGACCTTTACGGTCGTTCAAAATTTCTTGGTGAGGTAGATTATCCGCATGCTATCACATTGCGT
ACTTCCATTATAGGTCATGAGCTAGCAGGCAATAGAAGCTTAATCAATTGGTTTTTAAGTGCAGAAGGTTCAGTGAAAGG
GTTTGAGAAAGCTATTTATTCAGGATTCCCGACAGTAGAGCTTGCAAGAATAATAAGGGATTTTGTGTTACCTAATAAAG
AGTTGCGCGGGCTTTATCATGTTGCCTCAAAACCGATTAATAAGTTAGAATTGTTAAAATTGGTAGCAGAAATATATAAT
AAAAACATCGATATTATTTCATCAGATGAGCTTGTAGTAGATCGCTCACTAGATAGCACTCGTTTTAATGAGGTGACGGG
ATATACGCCTCCTGAATGGCGTGAGCTTGTAAAGCGTATGTGTGAGTTTAAGTAA

Upstream 100 bases:

>100_bases
TAATTATTTATAACTCAATATTGTTACGCACTCAAAACACTATTGTAAAGATATATTATTTATGATAGAACCGATTCGGT
AAAAATAAATAAAATATACT

Downstream 100 bases:

>100_bases
ATTGTCATTGCGAATATAGGCTTGTACACGGAATCCAGCATAAAGTGAGATAAATCGAGCTTTTTATGTTTGTATTTTTT
ATAACTGGACCCTGTGGACA

Product: dTDP-4-dehydrorhamnose reductase

Products: dTDP-4-dehydro-6-deoxy-L-mannose; NADPH; H+

Alternate protein names: Dtdp-4-Dehydrorhamnose Reductase; Carbohydrate Oxidoreductase; NAD-Dependent Epimerase/Dehydratase; Reductase; LPS Biosynthesis Related Reductase; TDP-4-Keto-L-Rhamnose Reductase.; DTDP-6-Deoxy-L-Mannose Dehydrogenase RmlD; Sugar Oxidoreductase; NAD Dependent Epimerase/Dehydratase

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MKILILGVTGMLGNSIFRFLSSDKKFDIFATARNNSARSYFSKDLSDKLITNVDVENHDALVEVFNQTKPDIVINCIGLV
KQLADANDPLKALPINSLLPHRLANLCKLANSRLIHISTDCVFSGKKGNYKESDFSDCYDLYGRSKFLGEVDYPHAITLR
TSIIGHELAGNRSLINWFLSAEGSVKGFEKAIYSGFPTVELARIIRDFVLPNKELRGLYHVASKPINKLELLKLVAEIYN
KNIDIISSDELVVDRSLDSTRFNEVTGYTPPEWRELVKRMCEFK

Sequences:

>Translated_284_residues
MKILILGVTGMLGNSIFRFLSSDKKFDIFATARNNSARSYFSKDLSDKLITNVDVENHDALVEVFNQTKPDIVINCIGLV
KQLADANDPLKALPINSLLPHRLANLCKLANSRLIHISTDCVFSGKKGNYKESDFSDCYDLYGRSKFLGEVDYPHAITLR
TSIIGHELAGNRSLINWFLSAEGSVKGFEKAIYSGFPTVELARIIRDFVLPNKELRGLYHVASKPINKLELLKLVAEIYN
KNIDIISSDELVVDRSLDSTRFNEVTGYTPPEWRELVKRMCEFK
>Mature_284_residues
MKILILGVTGMLGNSIFRFLSSDKKFDIFATARNNSARSYFSKDLSDKLITNVDVENHDALVEVFNQTKPDIVINCIGLV
KQLADANDPLKALPINSLLPHRLANLCKLANSRLIHISTDCVFSGKKGNYKESDFSDCYDLYGRSKFLGEVDYPHAITLR
TSIIGHELAGNRSLINWFLSAEGSVKGFEKAIYSGFPTVELARIIRDFVLPNKELRGLYHVASKPINKLELLKLVAEIYN
KNIDIISSDELVVDRSLDSTRFNEVTGYTPPEWRELVKRMCEFK

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 1.1.1.133

Molecular weight: Translated: 32039; Mature: 32039

Theoretical pI: Translated: 8.20; Mature: 8.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILILGVTGMLGNSIFRFLSSDKKFDIFATARNNSARSYFSKDLSDKLITNVDVENHDA
CEEEEEECHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCHHH
LVEVFNQTKPDIVINCIGLVKQLADANDPLKALPINSLLPHRLANLCKLANSRLIHISTD
HHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECC
CVFSGKKGNYKESDFSDCYDLYGRSKFLGEVDYPHAITLRTSIIGHELAGNRSLINWFLS
EEEECCCCCCCCCCHHHHHHHHCCHHHCCCCCCCEEEEEEHHHHHHHHCCCHHHHHHHHC
AEGSVKGFEKAIYSGFPTVELARIIRDFVLPNKELRGLYHVASKPINKLELLKLVAEIYN
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHC
KNIDIISSDELVVDRSLDSTRFNEVTGYTPPEWRELVKRMCEFK
CCCCEEECCCEEEECCCCCHHCCCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MKILILGVTGMLGNSIFRFLSSDKKFDIFATARNNSARSYFSKDLSDKLITNVDVENHDA
CEEEEEECHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCHHH
LVEVFNQTKPDIVINCIGLVKQLADANDPLKALPINSLLPHRLANLCKLANSRLIHISTD
HHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECC
CVFSGKKGNYKESDFSDCYDLYGRSKFLGEVDYPHAITLRTSIIGHELAGNRSLINWFLS
EEEECCCCCCCCCCHHHHHHHHCCHHHCCCCCCCEEEEEEHHHHHHHHCCCHHHHHHHHC
AEGSVKGFEKAIYSGFPTVELARIIRDFVLPNKELRGLYHVASKPINKLELLKLVAEIYN
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHC
KNIDIISSDELVVDRSLDSTRFNEVTGYTPPEWRELVKRMCEFK
CCCCEEECCCEEEECCCCCHHCCCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-6-deoxy-L-mannose; NADP+

Specific reaction: dTDP-6-deoxy-L-mannose + NADP+ = dTDP-4-dehydro-6-deoxy-L-mannose + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA