Click here to switch to the map view.

The map label for this gene is pdhB [H]

Identifier: 229586512

GI number: 229586512

Start: 352856

End: 353836

Strand: Direct

Name: pdhB [H]

Synonym: RAF_ORF0324

Alternate gene names: 229586512

Gene position: 352856-353836 (Clockwise)

Preceding gene: 229586511

Following gene: 229586514

Centisome position: 27.6

GC content: 39.14

Gene sequence:

>981_bases
ATGCAAATAACGGTACGTGAAGCATTGCGTGATGCCATGCAAGAAGAGATGATAAGAGACGACAAGGTTTTTGTCATAGG
CGAGGAAGTTGCAGAATATCAAGGAGCTTATAAGGTAACTCAAGGATTACTAGAGCAATTTGGTCCTAAGAGGGTAATTG
ATACACCGATAACGGAATATGGTTTTGCAGGGCTTGCAGTCGGAGCAGCTTTTGCAGGACTTCGCCCGATCGTAGAGTTT
ATGACCTTTAACTTTGCTATGCAAGCATTCGATCACATAGTTAATTCAGCTGCAAAAACGCATTACATGTCAGGCGGGCA
GGTCAAATGTCCGATAGTATTTAGAGGACCAAACGGAGCAGCAAGTAGAGTAGCTGCACAACATAGCCAAAATTATACAG
CTTGCTATTCCCACATTCCGGGATTAAAAGTAGTAGCTCCTTATAGTGCAGAAGATCATAAAGGACTTATGCTTACAGCT
ATTAGGGACGATAATCCCGTTGTTTTTTTAGAAAATGAAATTTTATACGGTCATAGTTTTGACGTGCCGAAAACAATCGA
GCCTATACCCTTTGGTCAGGCAAAAATTTTAAGAGAGGGTAGTAGTGTCACTATAGTAACTTTCTCGATTCAAGTAAAAC
TTGCCTTAGATGCTGCAAATGTTGTGCAGAATGATAATATTGATTGTGAGGTTATTGATCTTCGCACTATTAAACCGCTT
GATACTGAGACAATAATAGAATCAGTGAAAAAAACTAATCGTTTAGTTGTAGTGGAAGAAGGGTGGTTGTTTGCAGGTGT
TGGTGCAAGTATAGCTTCTATTGTTATGAAAGAAGCATTTGATTATTTAGATGCTCCGATAGAGATTGTAAGCGGCAAAG
ATCTTCCCCTTCCTTATGCTGTTAATTTAGAAATACTAGCTCTGCCGAGTGAGAGCGATGTAATAGAAGCCGTTAAAAAG
GTTTGTTATTATAGTATTTAA

Upstream 100 bases:

>100_bases
CATGAGATTGTCACGCAGCCTACGGCTGCTCGTAATGACGTTCACATAGGCAAGCCTCCTTGCAATAACGTTTAATTTAA
TCAAATAATAAAAAAGAAAA

Downstream 100 bases:

>100_bases
TGTATTAGCTTAGTTTTTTCGTCATTGCGAGCAACTGAAAGGAGCGTGGCAATCTCATAAAGCAAGATTCCAGAGATTGC
TTTGTCAAAACTTACAGTTT

Product: pyruvate dehydrogenase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MQITVREALRDAMQEEMIRDDKVFVIGEEVAEYQGAYKVTQGLLEQFGPKRVIDTPITEYGFAGLAVGAAFAGLRPIVEF
MTFNFAMQAFDHIVNSAAKTHYMSGGQVKCPIVFRGPNGAASRVAAQHSQNYTACYSHIPGLKVVAPYSAEDHKGLMLTA
IRDDNPVVFLENEILYGHSFDVPKTIEPIPFGQAKILREGSSVTIVTFSIQVKLALDAANVVQNDNIDCEVIDLRTIKPL
DTETIIESVKKTNRLVVVEEGWLFAGVGASIASIVMKEAFDYLDAPIEIVSGKDLPLPYAVNLEILALPSESDVIEAVKK
VCYYSI

Sequences:

>Translated_326_residues
MQITVREALRDAMQEEMIRDDKVFVIGEEVAEYQGAYKVTQGLLEQFGPKRVIDTPITEYGFAGLAVGAAFAGLRPIVEF
MTFNFAMQAFDHIVNSAAKTHYMSGGQVKCPIVFRGPNGAASRVAAQHSQNYTACYSHIPGLKVVAPYSAEDHKGLMLTA
IRDDNPVVFLENEILYGHSFDVPKTIEPIPFGQAKILREGSSVTIVTFSIQVKLALDAANVVQNDNIDCEVIDLRTIKPL
DTETIIESVKKTNRLVVVEEGWLFAGVGASIASIVMKEAFDYLDAPIEIVSGKDLPLPYAVNLEILALPSESDVIEAVKK
VCYYSI
>Mature_326_residues
MQITVREALRDAMQEEMIRDDKVFVIGEEVAEYQGAYKVTQGLLEQFGPKRVIDTPITEYGFAGLAVGAAFAGLRPIVEF
MTFNFAMQAFDHIVNSAAKTHYMSGGQVKCPIVFRGPNGAASRVAAQHSQNYTACYSHIPGLKVVAPYSAEDHKGLMLTA
IRDDNPVVFLENEILYGHSFDVPKTIEPIPFGQAKILREGSSVTIVTFSIQVKLALDAANVVQNDNIDCEVIDLRTIKPL
DTETIIESVKKTNRLVVVEEGWLFAGVGASIASIVMKEAFDYLDAPIEIVSGKDLPLPYAVNLEILALPSESDVIEAVKK
VCYYSI

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI156564403, Length=325, Percent_Identity=57.2307692307692, Blast_Score=382, Evalue=1e-106,
Organism=Homo sapiens, GI291084858, Length=325, Percent_Identity=53.8461538461538, Blast_Score=349, Evalue=2e-96,
Organism=Homo sapiens, GI4557353, Length=328, Percent_Identity=37.8048780487805, Blast_Score=215, Evalue=5e-56,
Organism=Homo sapiens, GI34101272, Length=328, Percent_Identity=37.8048780487805, Blast_Score=215, Evalue=5e-56,
Organism=Homo sapiens, GI225637461, Length=242, Percent_Identity=31.404958677686, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI225637463, Length=289, Percent_Identity=28.719723183391, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI225637459, Length=289, Percent_Identity=28.719723183391, Blast_Score=69, Evalue=7e-12,
Organism=Escherichia coli, GI1786622, Length=277, Percent_Identity=24.9097472924188, Blast_Score=72, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17538422, Length=323, Percent_Identity=60.9907120743034, Blast_Score=400, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17506935, Length=326, Percent_Identity=40.4907975460123, Blast_Score=211, Evalue=5e-55,
Organism=Saccharomyces cerevisiae, GI6319698, Length=326, Percent_Identity=60.7361963190184, Blast_Score=402, Evalue=1e-113,
Organism=Drosophila melanogaster, GI21358145, Length=325, Percent_Identity=57.5384615384615, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24650940, Length=325, Percent_Identity=57.5384615384615, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI160714832, Length=323, Percent_Identity=35.9133126934984, Blast_Score=209, Evalue=2e-54,
Organism=Drosophila melanogaster, GI160714828, Length=323, Percent_Identity=35.9133126934984, Blast_Score=209, Evalue=2e-54,
Organism=Drosophila melanogaster, GI24650943, Length=91, Percent_Identity=68.1318681318681, Blast_Score=143, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24650945, Length=91, Percent_Identity=68.1318681318681, Blast_Score=143, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24645119, Length=252, Percent_Identity=30.1587301587302, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI45551847, Length=252, Percent_Identity=30.1587301587302, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI45550715, Length=252, Percent_Identity=30.1587301587302, Blast_Score=75, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 35752; Mature: 35752

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQITVREALRDAMQEEMIRDDKVFVIGEEVAEYQGAYKVTQGLLEQFGPKRVIDTPITEY
CEEEHHHHHHHHHHHHHHCCCCEEEEEHHHHHHCCHHHHHHHHHHHCCCCCEECCCCHHH
GFAGLAVGAAFAGLRPIVEFMTFNFAMQAFDHIVNSAAKTHYMSGGQVKCPIVFRGPNGA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCH
ASRVAAQHSQNYTACYSHIPGLKVVAPYSAEDHKGLMLTAIRDDNPVVFLENEILYGHSF
HHHHHHHHCCCCHHHHHCCCCCEEEECCCCCCCCCEEEEEEECCCCEEEEECEEEECCCC
DVPKTIEPIPFGQAKILREGSSVTIVTFSIQVKLALDAANVVQNDNIDCEVIDLRTIKPL
CCCCCCCCCCCCHHHHHCCCCCEEEEEEEEEEEEEECHHHHHCCCCCCEEEEEEEECCCC
DTETIIESVKKTNRLVVVEEGWLFAGVGASIASIVMKEAFDYLDAPIEIVSGKDLPLPYA
CHHHHHHHHHHCCCEEEEECCCEEEECHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEE
VNLEILALPSESDVIEAVKKVCYYSI
ECEEEEEECCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MQITVREALRDAMQEEMIRDDKVFVIGEEVAEYQGAYKVTQGLLEQFGPKRVIDTPITEY
CEEEHHHHHHHHHHHHHHCCCCEEEEEHHHHHHCCHHHHHHHHHHHCCCCCEECCCCHHH
GFAGLAVGAAFAGLRPIVEFMTFNFAMQAFDHIVNSAAKTHYMSGGQVKCPIVFRGPNGA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCH
ASRVAAQHSQNYTACYSHIPGLKVVAPYSAEDHKGLMLTAIRDDNPVVFLENEILYGHSF
HHHHHHHHCCCCHHHHHCCCCCEEEECCCCCCCCCEEEEEEECCCCEEEEECEEEECCCC
DVPKTIEPIPFGQAKILREGSSVTIVTFSIQVKLALDAANVVQNDNIDCEVIDLRTIKPL
CCCCCCCCCCCCHHHHHCCCCCEEEEEEEEEEEEEECHHHHHCCCCCCEEEEEEEECCCC
DTETIIESVKKTNRLVVVEEGWLFAGVGASIASIVMKEAFDYLDAPIEIVSGKDLPLPYA
CHHHHHHHHHHCCCEEEEECCCEEEECHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEE
VNLEILALPSESDVIEAVKKVCYYSI
ECEEEEEECCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]