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The map label for this gene is thrS

Identifier: 229586468

GI number: 229586468

Start: 302594

End: 304501

Strand: Direct

Name: thrS

Synonym: RAF_ORF0276

Alternate gene names: 229586468

Gene position: 302594-304501 (Clockwise)

Preceding gene: 229586467

Following gene: 229586470

Centisome position: 23.67

GC content: 40.36

Gene sequence:

>1908_bases
ATGATTAATATTTCCTTTCCCGATGGGAGTATAAAACAATTTGCAAAAAACATTACCGCCTATGAAGTAGCAAATGCAAT
TTCAATGTCACTTGCTAAAGCAGCAATGGTTGCTGAAATAAACGGTGAGCTTCAAGACTTAAGTATCGTAATTGACAATG
ATTGCAAGCTTCGTATTTTAACTGCGAAAGATCCTGAATGTCTCGAGATAATAAGGCACGATGCGGCACATTTAACAGCA
GAAGCAGTAAAGGAACTATTCCCTGAAACTCAGGTAACGATTGGTCCTGCAATTGAAAACGGTTATTATTACGATTTTGC
TCGTGATAAACCTTTTACCACTGATGATTTAGCCGTTATAGAAGCTAAAATGCAAGAACTTTCACAAAAGAACGAGCAAG
TAACAAGAGAGCTATGGGATAGGGATAAAGCCGTAGAGTTTTTTAAATCGATAGGAGAGCATTATAAAGCCGAAATTATT
GCTTCAATTCCGGCAGGTGAGCCAATTACTTTATACAGGCAAGGTAATTTTATTGATCTATGCCGAGGTCCGCATGCTCC
TTCTACAGGGGTTGTTAAGCATTTTAAGTTGATGAAAGTTGCAGGAGCGTATTGGCGAGGTGATAGTCGTAATGAGATGT
TGCAGCGTATATACGGCACTGCTTGGGCCACGAAGGAGCAGCTAGATAGTTACCTCTTGATGCTTGAAGAAGCCGAAAAG
CGTGATCATAGAAAGCTTGGGCGTGAGCTTGATTTATTCCACTTCCAAGAAGAAGCTCAAGGAATGGTATTTTGGCATGA
TAAAGGCTGGAGTATATATAATACCATTGAGCAGTATATTAGGAAAAAGATTCGTAAAAACGGTTATACCGAAGTTAAGA
CTCCTGTTTTGGTCGATAAAAGCCTTTGGGAGGCTTCAGGACATTGGGAAAAGTTTCGTGATGATATGTTTGCGTTAGAA
ACAGACGACAAGACATTAGCTTTGAAGCCGATGAACTGCCCTTGCCACGTGCAGATTTTCAAACAAGGTATCAAGAGTTA
TCGTGATTTACCTCTTCGTATGTCGGAGTTTGGTTTGTGCCATCGTAATGAGGCATCCGGTGCATTGCACGGTTTAATGA
GAGTGCGGAGTTTAGTGCAAGACGATGCACATATATTTTGTGCTGCGGAACAAATTACCGATGAAACGGTTAGTTTTTGT
AAATTACTGACTGAAGTTTATAAGGATTTCGGCTTTACTGATATAAAGGTAAAATTCTCTGATCGTCCTGAAATCAGAGC
AGGGAGCAATGAAGTTTGGGATAAGGCTGAGAATGCTTTAAAAGAAGCAGTAGAACAAGCAGGGTTTAATTATACACTAA
ACCCGGGCGAGGGGGCATTTTACGGTCCGAAGCTTGAGTTTGTATTAACCGATGCGATAGGGCGGCAATGGCAATGCGGT
ACGCTTCAGATGGATTTTGTTTTGCCGGAGCGGTTAGACGCTAGCTATGTTGCAGCAAGCGGAGAGAAAAAAAGACCCGT
AATGCTACATAGAGCGATCCTTGGCTCACTTGAGCGTTTTATTGGTATATTGATCGAAGAATATGCAGGTAGGTTTCCGC
TTTGGCTTGCACCAGTGCAGGTTGCTATTGCAACTATTACCAGCGATTTGAACGATTACGCTTTGGAAGTGCAGAAAGCT
TTAATTGATAACGGGATAAGAACGGATTTTAATATCTCACCTGATAAAATTAACTATAAGATTCGTGAGTTTTCTAATCA
AAAAATACCGATGATTGCAGTAATTGGTAAACAAGAGCAAGAAAATAAACAAGTAGCGATTAGAAGGCTCGGAACTACTG
ACCAAGAAGTATTATCGGTTGAGCAGTTAATAGCTGTGGTTAAAGAAGAGAATGAGAAATATTTGTAG

Upstream 100 bases:

>100_bases
AGTAGTCTAAGTGTCATTCCTGCTTTTGCAGGAATGACATAAGAGCTACGAGATAAGGTCTACAAGTAAAAAATTTGAAG
AAAAAATAAGTTATAAAAGA

Downstream 100 bases:

>100_bases
ATTTTAAATTTAAAGGATGATATAAGATAATAATACGTATCATAGGGATATGATATCCTAATTAGGAAAAAATCCTAAGG
AGATTATGTGAAAACTATTA

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS

Number of amino acids: Translated: 635; Mature: 635

Protein sequence:

>635_residues
MINISFPDGSIKQFAKNITAYEVANAISMSLAKAAMVAEINGELQDLSIVIDNDCKLRILTAKDPECLEIIRHDAAHLTA
EAVKELFPETQVTIGPAIENGYYYDFARDKPFTTDDLAVIEAKMQELSQKNEQVTRELWDRDKAVEFFKSIGEHYKAEII
ASIPAGEPITLYRQGNFIDLCRGPHAPSTGVVKHFKLMKVAGAYWRGDSRNEMLQRIYGTAWATKEQLDSYLLMLEEAEK
RDHRKLGRELDLFHFQEEAQGMVFWHDKGWSIYNTIEQYIRKKIRKNGYTEVKTPVLVDKSLWEASGHWEKFRDDMFALE
TDDKTLALKPMNCPCHVQIFKQGIKSYRDLPLRMSEFGLCHRNEASGALHGLMRVRSLVQDDAHIFCAAEQITDETVSFC
KLLTEVYKDFGFTDIKVKFSDRPEIRAGSNEVWDKAENALKEAVEQAGFNYTLNPGEGAFYGPKLEFVLTDAIGRQWQCG
TLQMDFVLPERLDASYVAASGEKKRPVMLHRAILGSLERFIGILIEEYAGRFPLWLAPVQVAIATITSDLNDYALEVQKA
LIDNGIRTDFNISPDKINYKIREFSNQKIPMIAVIGKQEQENKQVAIRRLGTTDQEVLSVEQLIAVVKEENEKYL

Sequences:

>Translated_635_residues
MINISFPDGSIKQFAKNITAYEVANAISMSLAKAAMVAEINGELQDLSIVIDNDCKLRILTAKDPECLEIIRHDAAHLTA
EAVKELFPETQVTIGPAIENGYYYDFARDKPFTTDDLAVIEAKMQELSQKNEQVTRELWDRDKAVEFFKSIGEHYKAEII
ASIPAGEPITLYRQGNFIDLCRGPHAPSTGVVKHFKLMKVAGAYWRGDSRNEMLQRIYGTAWATKEQLDSYLLMLEEAEK
RDHRKLGRELDLFHFQEEAQGMVFWHDKGWSIYNTIEQYIRKKIRKNGYTEVKTPVLVDKSLWEASGHWEKFRDDMFALE
TDDKTLALKPMNCPCHVQIFKQGIKSYRDLPLRMSEFGLCHRNEASGALHGLMRVRSLVQDDAHIFCAAEQITDETVSFC
KLLTEVYKDFGFTDIKVKFSDRPEIRAGSNEVWDKAENALKEAVEQAGFNYTLNPGEGAFYGPKLEFVLTDAIGRQWQCG
TLQMDFVLPERLDASYVAASGEKKRPVMLHRAILGSLERFIGILIEEYAGRFPLWLAPVQVAIATITSDLNDYALEVQKA
LIDNGIRTDFNISPDKINYKIREFSNQKIPMIAVIGKQEQENKQVAIRRLGTTDQEVLSVEQLIAVVKEENEKYL
>Mature_635_residues
MINISFPDGSIKQFAKNITAYEVANAISMSLAKAAMVAEINGELQDLSIVIDNDCKLRILTAKDPECLEIIRHDAAHLTA
EAVKELFPETQVTIGPAIENGYYYDFARDKPFTTDDLAVIEAKMQELSQKNEQVTRELWDRDKAVEFFKSIGEHYKAEII
ASIPAGEPITLYRQGNFIDLCRGPHAPSTGVVKHFKLMKVAGAYWRGDSRNEMLQRIYGTAWATKEQLDSYLLMLEEAEK
RDHRKLGRELDLFHFQEEAQGMVFWHDKGWSIYNTIEQYIRKKIRKNGYTEVKTPVLVDKSLWEASGHWEKFRDDMFALE
TDDKTLALKPMNCPCHVQIFKQGIKSYRDLPLRMSEFGLCHRNEASGALHGLMRVRSLVQDDAHIFCAAEQITDETVSFC
KLLTEVYKDFGFTDIKVKFSDRPEIRAGSNEVWDKAENALKEAVEQAGFNYTLNPGEGAFYGPKLEFVLTDAIGRQWQCG
TLQMDFVLPERLDASYVAASGEKKRPVMLHRAILGSLERFIGILIEEYAGRFPLWLAPVQVAIATITSDLNDYALEVQKA
LIDNGIRTDFNISPDKINYKIREFSNQKIPMIAVIGKQEQENKQVAIRRLGTTDQEVLSVEQLIAVVKEENEKYL

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI38202255, Length=620, Percent_Identity=39.8387096774194, Blast_Score=473, Evalue=1e-133,
Organism=Homo sapiens, GI38570111, Length=612, Percent_Identity=39.2156862745098, Blast_Score=471, Evalue=1e-132,
Organism=Homo sapiens, GI20070344, Length=639, Percent_Identity=39.2801251956182, Blast_Score=442, Evalue=1e-124,
Organism=Escherichia coli, GI1788013, Length=631, Percent_Identity=51.0301109350238, Blast_Score=685, Evalue=0.0,
Organism=Escherichia coli, GI1786392, Length=158, Percent_Identity=24.0506329113924, Blast_Score=69, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI71984184, Length=638, Percent_Identity=40.1253918495298, Blast_Score=497, Evalue=1e-141,
Organism=Caenorhabditis elegans, GI71984192, Length=638, Percent_Identity=40.1253918495298, Blast_Score=497, Evalue=1e-141,
Organism=Saccharomyces cerevisiae, GI6322112, Length=651, Percent_Identity=37.3271889400922, Blast_Score=464, Evalue=1e-131,
Organism=Saccharomyces cerevisiae, GI6322654, Length=458, Percent_Identity=36.4628820960699, Blast_Score=281, Evalue=2e-76,
Organism=Drosophila melanogaster, GI24583839, Length=637, Percent_Identity=39.7174254317111, Blast_Score=499, Evalue=1e-141,
Organism=Drosophila melanogaster, GI24583842, Length=637, Percent_Identity=39.7174254317111, Blast_Score=499, Evalue=1e-141,
Organism=Drosophila melanogaster, GI28574141, Length=637, Percent_Identity=39.7174254317111, Blast_Score=499, Evalue=1e-141,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYT_RICAE (C3PMR6)

Other databases:

- EMBL:   CP001612
- RefSeq:   YP_002844969.1
- ProteinModelPortal:   C3PMR6
- GeneID:   7815277
- GenomeReviews:   CP001612_GR
- KEGG:   raf:RAF_ORF0276
- OMA:   TAKDPEC
- ProtClustDB:   PRK00413
- GO:   GO:0005737
- HAMAP:   MF_00184_B
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR004095
- InterPro:   IPR012676
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.10.20.30
- PRINTS:   PR01047
- SMART:   SM00863
- TIGRFAMs:   TIGR00418

Pfam domain/function: PF03129 HGTP_anticodon; PF02824 TGS; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD; SSF52954 Anticodon_bd; SSF81271 TGS-like; SSF55186 Thr/Ala-tRNA-synth_IIc_edit

EC number: =6.1.1.3

Molecular weight: Translated: 72367; Mature: 72367

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINISFPDGSIKQFAKNITAYEVANAISMSLAKAAMVAEINGELQDLSIVIDNDCKLRIL
CEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCEEEEE
TAKDPECLEIIRHDAAHLTAEAVKELFPETQVTIGPAIENGYYYDFARDKPFTTDDLAVI
ECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCEECCCEEEEECCCCCCCCCHHHHH
EAKMQELSQKNEQVTRELWDRDKAVEFFKSIGEHYKAEIIASIPAGEPITLYRQGNFIDL
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHEEEEEEECCCCCCEEEEECCCEEEE
CRGPHAPSTGVVKHFKLMKVAGAYWRGDSRNEMLQRIYGTAWATKEQLDSYLLMLEEAEK
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
RDHRKLGRELDLFHFQEEAQGMVFWHDKGWSIYNTIEQYIRKKIRKNGYTEVKTPVLVDK
HHHHHHCCCCCHHEEHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEECH
SLWEASGHWEKFRDDMFALETDDKTLALKPMNCPCHVQIFKQGIKSYRDLPLRMSEFGLC
HHHHCCCCHHHHHCCCEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCHHHCCCC
HRNEASGALHGLMRVRSLVQDDAHIFCAAEQITDETVSFCKLLTEVYKDFGFTDIKVKFS
CCCCCHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEC
DRPEIRAGSNEVWDKAENALKEAVEQAGFNYTLNPGEGAFYGPKLEFVLTDAIGRQWQCG
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCEECCEEEEEEEHHCCCCCCCC
TLQMDFVLPERLDASYVAASGEKKRPVMLHRAILGSLERFIGILIEEYAGRFPLWLAPVQ
CEEEEEECCCCCCCHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHH
VAIATITSDLNDYALEVQKALIDNGIRTDFNISPDKINYKIREFSNQKIPMIAVIGKQEQ
HHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCCCHHHHCEEEECCCCCCCCEEEEECCCCC
ENKQVAIRRLGTTDQEVLSVEQLIAVVKEENEKYL
CCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MINISFPDGSIKQFAKNITAYEVANAISMSLAKAAMVAEINGELQDLSIVIDNDCKLRIL
CEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCEEEEE
TAKDPECLEIIRHDAAHLTAEAVKELFPETQVTIGPAIENGYYYDFARDKPFTTDDLAVI
ECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCEECCCEEEEECCCCCCCCCHHHHH
EAKMQELSQKNEQVTRELWDRDKAVEFFKSIGEHYKAEIIASIPAGEPITLYRQGNFIDL
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHEEEEEEECCCCCCEEEEECCCEEEE
CRGPHAPSTGVVKHFKLMKVAGAYWRGDSRNEMLQRIYGTAWATKEQLDSYLLMLEEAEK
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
RDHRKLGRELDLFHFQEEAQGMVFWHDKGWSIYNTIEQYIRKKIRKNGYTEVKTPVLVDK
HHHHHHCCCCCHHEEHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEECH
SLWEASGHWEKFRDDMFALETDDKTLALKPMNCPCHVQIFKQGIKSYRDLPLRMSEFGLC
HHHHCCCCHHHHHCCCEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCHHHCCCC
HRNEASGALHGLMRVRSLVQDDAHIFCAAEQITDETVSFCKLLTEVYKDFGFTDIKVKFS
CCCCCHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEC
DRPEIRAGSNEVWDKAENALKEAVEQAGFNYTLNPGEGAFYGPKLEFVLTDAIGRQWQCG
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCEECCEEEEEEEHHCCCCCCCC
TLQMDFVLPERLDASYVAASGEKKRPVMLHRAILGSLERFIGILIEEYAGRFPLWLAPVQ
CEEEEEECCCCCCCHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHH
VAIATITSDLNDYALEVQKALIDNGIRTDFNISPDKINYKIREFSNQKIPMIAVIGKQEQ
HHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCCCHHHHCEEEECCCCCCCCEEEEECCCCC
ENKQVAIRRLGTTDQEVLSVEQLIAVVKEENEKYL
CCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA