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The map label for this gene is purC

Identifier: 229586466

GI number: 229586466

Start: 299981

End: 300691

Strand: Direct

Name: purC

Synonym: RAF_ORF0274

Alternate gene names: 229586466

Gene position: 299981-300691 (Clockwise)

Preceding gene: 229586465

Following gene: 229586467

Centisome position: 23.46

GC content: 29.96

Gene sequence:

>711_bases
ATGAAAAAAAAATTATATGAAGGTTCAAGTAAAATTTTATATTCTGCCGAAGAAGATTTTTTACTTATAATGGCTTTTTC
CGATAAGGCTATTTTAGAAACAGGTGAGATTGTTGATATATCAGGTAAAGGAGTACTTAATAATAATATTTCTTCCTTTC
TAATGGATAAACTGGAAATGATCGGCATTGAAAATCATTTTATCGAAAAAATAAATATGAGGGAGCAATTAATTCAATAT
GTTGAGGTTTTCCCGATACAAGTAATAATATCATCAGTAGCGTGCAGTAGATTTGTAAAAGAATTCGGAATTGATGAAGG
GTATGTATTTGATAAACCTATTATTGATTTTAAGGTACGAAGTCGTGAATTTAAATATCCGATAGTTAATGAGTATCAAA
TATTAAATTTCGGTTGGTTAACTAGGGATGAAATTAAAGCGGTAAAAGAGCAGGCTTTGCGTATATATGATTTTCTAAGC
GGCTTATTTATCGGTGTTGGGATTCGGCTTGTTGAGTGTAAATTAGAATTTGGGCGTGTGTTTAACGGTGAAGAATCTAT
CATTATGTTAACTGATGAAATTAGTCCGGATAATTGTAGATTATGGCATATTAACAGTAATGAAAAATTGGGATTTGAAT
TGCTTGAAAAAGAACCAAATAAGGTTTTTGAATCATATCAGCTAATAGCTGATCGTTTAAAAGAAAAATAA

Upstream 100 bases:

>100_bases
TAGCAATCTCATGAAATAATAACAAGCTCCTGAGATTGCTTCGTCAATTACTTCGTAATTTTCTCGCAATGACGAGGAAA
ATGAATTTTGAGACAAATAA

Downstream 100 bases:

>100_bases
ATATATTTTTTAAGCGATATATACTATTTTGGACTGTTTAAGGTTTTATTACAAAGTTCAGTTTTTCGTCATTGCGAGGA
AAATTACGAAGTAATGGACA

Product: phosphoribosylaminoimidazole-succinocarboxamide synthase

Products: NA

Alternate protein names: SAICAR synthetase

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MKKKLYEGSSKILYSAEEDFLLIMAFSDKAILETGEIVDISGKGVLNNNISSFLMDKLEMIGIENHFIEKINMREQLIQY
VEVFPIQVIISSVACSRFVKEFGIDEGYVFDKPIIDFKVRSREFKYPIVNEYQILNFGWLTRDEIKAVKEQALRIYDFLS
GLFIGVGIRLVECKLEFGRVFNGEESIIMLTDEISPDNCRLWHINSNEKLGFELLEKEPNKVFESYQLIADRLKEK

Sequences:

>Translated_236_residues
MKKKLYEGSSKILYSAEEDFLLIMAFSDKAILETGEIVDISGKGVLNNNISSFLMDKLEMIGIENHFIEKINMREQLIQY
VEVFPIQVIISSVACSRFVKEFGIDEGYVFDKPIIDFKVRSREFKYPIVNEYQILNFGWLTRDEIKAVKEQALRIYDFLS
GLFIGVGIRLVECKLEFGRVFNGEESIIMLTDEISPDNCRLWHINSNEKLGFELLEKEPNKVFESYQLIADRLKEK
>Mature_236_residues
MKKKLYEGSSKILYSAEEDFLLIMAFSDKAILETGEIVDISGKGVLNNNISSFLMDKLEMIGIENHFIEKINMREQLIQY
VEVFPIQVIISSVACSRFVKEFGIDEGYVFDKPIIDFKVRSREFKYPIVNEYQILNFGWLTRDEIKAVKEQALRIYDFLS
GLFIGVGIRLVECKLEFGRVFNGEESIIMLTDEISPDNCRLWHINSNEKLGFELLEKEPNKVFESYQLIADRLKEK

Specific function: De novo purine biosynthesis; seventh step. [C]

COG id: COG0152

COG function: function code F; Phosphoribosylaminoimidazolesuccinocarboxamide (SAICAR) synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SAICAR synthetase family

Homologues:

Organism=Escherichia coli, GI1788820, Length=233, Percent_Identity=31.3304721030043, Blast_Score=129, Evalue=2e-31,

Paralogues:

None

Copy number: 1680 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 13,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PUR7_RICAE (C3PMR4)

Other databases:

- EMBL:   CP001612
- RefSeq:   YP_002844967.1
- ProteinModelPortal:   C3PMR4
- SMR:   C3PMR4
- GeneID:   7815275
- GenomeReviews:   CP001612_GR
- KEGG:   raf:RAF_ORF0274
- OMA:   YKDDALG
- ProtClustDB:   PRK09362
- HAMAP:   MF_00137
- InterPro:   IPR013816
- InterPro:   IPR001636
- Gene3D:   G3DSA:3.30.470.20
- PANTHER:   PTHR11609

Pfam domain/function: PF01259 SAICAR_synt

EC number: =6.3.2.6

Molecular weight: Translated: 27436; Mature: 27436

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS01057 SAICAR_SYNTHETASE_1; PS01058 SAICAR_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKLYEGSSKILYSAEEDFLLIMAFSDKAILETGEIVDISGKGVLNNNISSFLMDKLEM
CCCCCCCCCCEEEEECCCCEEEEEEECCCEEECCCCEEEECCCCEECCCHHHHHHHHHHH
IGIENHFIEKINMREQLIQYVEVFPIQVIISSVACSRFVKEFGIDEGYVFDKPIIDFKVR
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEEC
SREFKYPIVNEYQILNFGWLTRDEIKAVKEQALRIYDFLSGLFIGVGIRLVECKLEFGRV
CCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEEECCCE
FNGEESIIMLTDEISPDNCRLWHINSNEKLGFELLEKEPNKVFESYQLIADRLKEK
ECCCCCEEEEEECCCCCCEEEEEECCCCCCCHHHHHCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKKLYEGSSKILYSAEEDFLLIMAFSDKAILETGEIVDISGKGVLNNNISSFLMDKLEM
CCCCCCCCCCEEEEECCCCEEEEEEECCCEEECCCCEEEECCCCEECCCHHHHHHHHHHH
IGIENHFIEKINMREQLIQYVEVFPIQVIISSVACSRFVKEFGIDEGYVFDKPIIDFKVR
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEEC
SREFKYPIVNEYQILNFGWLTRDEIKAVKEQALRIYDFLSGLFIGVGIRLVECKLEFGRV
CCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEEECCCE
FNGEESIIMLTDEISPDNCRLWHINSNEKLGFELLEKEPNKVFESYQLIADRLKEK
ECCCCCEEEEEECCCCCCEEEEEECCCCCCCHHHHHCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA