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The map label for this gene is rnhB [H]

Identifier: 229586446

GI number: 229586446

Start: 270429

End: 271010

Strand: Direct

Name: rnhB [H]

Synonym: RAF_ORF0247

Alternate gene names: 229586446

Gene position: 270429-271010 (Clockwise)

Preceding gene: 229586438

Following gene: 229586448

Centisome position: 21.15

GC content: 34.19

Gene sequence:

>582_bases
ATGGAAGTAGATCTATTACATTTTGAAAAAAAATATCATAACTGTATTGTAGCAGGTATAGATGAAGCAGGTAGAGGACC
GCTTGCAGGTCCGGTAGTAGCAAGTGCCGTTATAGTAGATAACGCCAATATTATAACGGGAATTAAAGATTCTAAAAAGC
TTTCCAAAAAGAAAAGAGAATTACTATATGAACAAATCACTAGTAATTATGTTTGGGCAACTGCTATTATTTCTCATACC
GAAATTGATGATATTAATATATTAGAGGCAACTAAAAAAGCTTGTTCTATTGCTGTAGCAAATCTTAGCCTTGAGCCAGA
AATAGTGTTAGTTGACGGTAATATGCAGTTTAAGGATGAAAGATTTGTCAGTATAATTAACGGTGATAATTTATCATTAT
CGATTGCTGCGGCTTCTATTGTTGCAAAAGTTACTAGGGATCGTTTGATGCTAGACTTAAGTGCTGAATTTCCCCAATAT
TTATGGCACAAAAATTCCGGCTACGGTACCAAAGAACATATCGAAGCTATTAATATATATGGCTTATCACCGTATCATCG
TAGGAGTTTTAGGTGTTGTTAA

Upstream 100 bases:

>100_bases
CTCTTGCGGAAGTCCTAATAATTGAAAATAATTTTGCACTTTTCCTTTTTTGTAATTCTAAACTGCTCTATCATATATCA
AAAATTAAAAAAATACAATT

Downstream 100 bases:

>100_bases
TGGTATTGCCCGTGTGGACACTGAATAGTCATTGCGAGCAGCCATAGGCTGCGTGGCAATCTCATGAAATAATAACACAC
TCCTGAGATTGTCAAAACTT

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII [H]

Number of amino acids: Translated: 193; Mature: 193

Protein sequence:

>193_residues
MEVDLLHFEKKYHNCIVAGIDEAGRGPLAGPVVASAVIVDNANIITGIKDSKKLSKKKRELLYEQITSNYVWATAIISHT
EIDDINILEATKKACSIAVANLSLEPEIVLVDGNMQFKDERFVSIINGDNLSLSIAAASIVAKVTRDRLMLDLSAEFPQY
LWHKNSGYGTKEHIEAINIYGLSPYHRRSFRCC

Sequences:

>Translated_193_residues
MEVDLLHFEKKYHNCIVAGIDEAGRGPLAGPVVASAVIVDNANIITGIKDSKKLSKKKRELLYEQITSNYVWATAIISHT
EIDDINILEATKKACSIAVANLSLEPEIVLVDGNMQFKDERFVSIINGDNLSLSIAAASIVAKVTRDRLMLDLSAEFPQY
LWHKNSGYGTKEHIEAINIYGLSPYHRRSFRCC
>Mature_193_residues
MEVDLLHFEKKYHNCIVAGIDEAGRGPLAGPVVASAVIVDNANIITGIKDSKKLSKKKRELLYEQITSNYVWATAIISHT
EIDDINILEATKKACSIAVANLSLEPEIVLVDGNMQFKDERFVSIINGDNLSLSIAAASIVAKVTRDRLMLDLSAEFPQY
LWHKNSGYGTKEHIEAINIYGLSPYHRRSFRCC

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids [H]

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family [H]

Homologues:

Organism=Escherichia coli, GI1786380, Length=180, Percent_Identity=47.2222222222222, Blast_Score=153, Evalue=6e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01351 RNase_HII [H]

EC number: =3.1.26.4 [H]

Molecular weight: Translated: 21464; Mature: 21464

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVDLLHFEKKYHNCIVAGIDEAGRGPLAGPVVASAVIVDNANIITGIKDSKKLSKKKRE
CCCCHHHHHHHHCCEEEEECCCCCCCCCCCHHHEEEEEEECCEEEECCCCHHHHHHHHHH
LLYEQITSNYVWATAIISHTEIDDINILEATKKACSIAVANLSLEPEIVLVDGNMQFKDE
HHHHHHHCCEEEEEEEEECCCCCCCHHHHHHHHHHEEEEEECCCCCEEEEEECCCEECCC
RFVSIINGDNLSLSIAAASIVAKVTRDRLMLDLSAEFPQYLWHKNSGYGTKEHIEAINIY
EEEEEECCCCEEEEHHHHHHHHHHHHHHEEEEECCCCCHHHHCCCCCCCCHHHHEEEEEE
GLSPYHRRSFRCC
CCCHHHCCCCCCC
>Mature Secondary Structure
MEVDLLHFEKKYHNCIVAGIDEAGRGPLAGPVVASAVIVDNANIITGIKDSKKLSKKKRE
CCCCHHHHHHHHCCEEEEECCCCCCCCCCCHHHEEEEEEECCEEEECCCCHHHHHHHHHH
LLYEQITSNYVWATAIISHTEIDDINILEATKKACSIAVANLSLEPEIVLVDGNMQFKDE
HHHHHHHCCEEEEEEEEECCCCCCCHHHHHHHHHHEEEEEECCCCCEEEEEECCCEECCC
RFVSIINGDNLSLSIAAASIVAKVTRDRLMLDLSAEFPQYLWHKNSGYGTKEHIEAINIY
EEEEEECCCCEEEEHHHHHHHHHHHHHHEEEEECCCCCHHHHCCCCCCCCHHHHEEEEEE
GLSPYHRRSFRCC
CCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA