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The map label for this gene is aspS

Identifier: 229586385

GI number: 229586385

Start: 193369

End: 195177

Strand: Direct

Name: aspS

Synonym: RAF_ORF0176

Alternate gene names: 229586385

Gene position: 193369-195177 (Clockwise)

Preceding gene: 229586384

Following gene: 229586399

Centisome position: 15.12

GC content: 38.81

Gene sequence:

>1809_bases
ATGCATAAATACAGAACTCATAATTGTAACGAATTACAAATATCCGATGTAGAAACAGAAGTTAAGCTATCCGGTTGGGT
ACATAGAAGAAGAGATCACGGGAATCTAGTTTTTATAGATTTGCGTGATCATTACGGTATAACTCAAATCGTGTTTACCG
ATCAAAATCCTCAGCTTATGGAGGATGCAAGTCGTTTGCGTTATGAGTCGGTAATTACGGTAAGGGGTACAGTTGTAGCA
AGATCAGAGGACACGATTAATAATACGCTTCCGACAGGTCATGTTGAAGTGCTAGCTGTGGAATTTAGTGTTGAGTCAGC
TGCGGACACTCTACCTTTTGTTATTAATACTGAAAAAGACGCTCCCGAAGAGTCTAGGTTTAAACATCGCTTTTTAGATC
TTAGGCGTGAGAAATTACATAATAATATAATACTCCGCTCACAAATTATTTCTCATATTCGTCATTTGATGACGGCAAGC
GGTTTTACCGAATTTCAAACTCCGATTTTAACAGCAAGCTCACCTGAGGGGGCTAGGGATTTTTTAGTACCTAGTAGAAT
GCATCCGGGTAAGTTCTATGCATTACCGCAAGCACCTCAGCAATTCAAGCAGTTATTGATGGTATCAGGATTTGATCGTT
ATTTCCAAATTGCTCCTTGTTTTCGGGATGAAGATGCAAGAGCTGATAGATCACCCGGCGAGTTTTATCAGCTAGATGTA
GAAATGTCGTTTGTTACACAGGAAGACGTATTTAGTACTATTGAGCCTGTGATGTATGATTTATTCACTAAATTCACGGA
TAAGAAAGTATCAGAGACTCCTTTTGTTTGTATTCCATATAATGAATCTATGTTAAAATATGGCTCTGATAAGCCTGATT
TGCGTAACCCTATTATAATTGCCGATGTAACTGAGATATTTAGAGATTCCGATTTTACAATCTTTAGAGAGAATATTAAA
AAAGGTAGCATAGTGCGTGCAATTCCTGCACCTAAGGCAGCTGCACTGCCTCGTAGTTTCTTCGATAAAATGATAGAGTT
TGCGGTATCAGAAGGAGCAGGTGGACTTGGCTATATTCAGTTTAGTGAAACCGGTGAAGCAAAGGGACCGGTAGTAAAAT
TCTTAAGCCCGCAGCAATTAGAGAGTCTAAAAGCTACAGCTAGTATTAGTAACGGTGATGCTGTGTTTTTCGCTAGCGAT
AAGAAAGAGAAAGCTGCTAAACTTGCCGGTAAGGTTAGAATAAGACTTGGTGAAGAGCTTGATTTACTTGAAAAAGATTG
TTTTAAATTCTGCTGGATTACCGATTTTCCATTCTATGAGTTAAATGAAGAAACAGGAAAAATTGATTTTAGCCATAATC
CGTTTTCTATGCCGCAGGGCGGAATAGATGCTTTAGAGCAAGCTAAAACCACTGAAGAGTTATTAGAACTTACCGCATAC
CAATATGATATTGTTTGTAACGGTATTGAACTGTCCAGCGGTGCTATTAGAAACCATAAGCCGGAAATTATGTATAAAGC
ATTTTCCATAGCAGGTTATAGTGAAGAGGAAGTTGATAAGAGATTTGGAGGGATGATTAGAGCATTTAAGTTTGGAGCTC
CACCACATGGTGGAATAGCCCCCGGTATTGACCGAATCGTTATGCTGCTTGCGGAAGCAACAAATATAAGGGAAATAATT
GCGTTTCCTTTAAACCAGCAGGCTGAAGATTTACTAATGAACGCCCCAAGCTATGTAGAGGATAAAGCTCTAAAAGAGCT
AAGCATTATGCTCTCACCGTCTGCTAGAAAAAATGCAGAAAAAGAATAA

Upstream 100 bases:

>100_bases
ATCAAGATAAAACTTTGGCAAGATGAATTTTTAGTTTTTATTTGCGAAAATTTGTGATTTTATGTATATAATGGGGAAAT
CGTATAATAAAAAAATAAAA

Downstream 100 bases:

>100_bases
GAATTTATAGTTGTTGTATGTTATTATGTCATACCGCAACTTGGCCTATAGTATGTCATTTCTAGCTAAAAGCGGAAATC
CAGTACTTTTCGCTGTCATA

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 602; Mature: 602

Protein sequence:

>602_residues
MHKYRTHNCNELQISDVETEVKLSGWVHRRRDHGNLVFIDLRDHYGITQIVFTDQNPQLMEDASRLRYESVITVRGTVVA
RSEDTINNTLPTGHVEVLAVEFSVESAADTLPFVINTEKDAPEESRFKHRFLDLRREKLHNNIILRSQIISHIRHLMTAS
GFTEFQTPILTASSPEGARDFLVPSRMHPGKFYALPQAPQQFKQLLMVSGFDRYFQIAPCFRDEDARADRSPGEFYQLDV
EMSFVTQEDVFSTIEPVMYDLFTKFTDKKVSETPFVCIPYNESMLKYGSDKPDLRNPIIIADVTEIFRDSDFTIFRENIK
KGSIVRAIPAPKAAALPRSFFDKMIEFAVSEGAGGLGYIQFSETGEAKGPVVKFLSPQQLESLKATASISNGDAVFFASD
KKEKAAKLAGKVRIRLGEELDLLEKDCFKFCWITDFPFYELNEETGKIDFSHNPFSMPQGGIDALEQAKTTEELLELTAY
QYDIVCNGIELSSGAIRNHKPEIMYKAFSIAGYSEEEVDKRFGGMIRAFKFGAPPHGGIAPGIDRIVMLLAEATNIREII
AFPLNQQAEDLLMNAPSYVEDKALKELSIMLSPSARKNAEKE

Sequences:

>Translated_602_residues
MHKYRTHNCNELQISDVETEVKLSGWVHRRRDHGNLVFIDLRDHYGITQIVFTDQNPQLMEDASRLRYESVITVRGTVVA
RSEDTINNTLPTGHVEVLAVEFSVESAADTLPFVINTEKDAPEESRFKHRFLDLRREKLHNNIILRSQIISHIRHLMTAS
GFTEFQTPILTASSPEGARDFLVPSRMHPGKFYALPQAPQQFKQLLMVSGFDRYFQIAPCFRDEDARADRSPGEFYQLDV
EMSFVTQEDVFSTIEPVMYDLFTKFTDKKVSETPFVCIPYNESMLKYGSDKPDLRNPIIIADVTEIFRDSDFTIFRENIK
KGSIVRAIPAPKAAALPRSFFDKMIEFAVSEGAGGLGYIQFSETGEAKGPVVKFLSPQQLESLKATASISNGDAVFFASD
KKEKAAKLAGKVRIRLGEELDLLEKDCFKFCWITDFPFYELNEETGKIDFSHNPFSMPQGGIDALEQAKTTEELLELTAY
QYDIVCNGIELSSGAIRNHKPEIMYKAFSIAGYSEEEVDKRFGGMIRAFKFGAPPHGGIAPGIDRIVMLLAEATNIREII
AFPLNQQAEDLLMNAPSYVEDKALKELSIMLSPSARKNAEKE
>Mature_602_residues
MHKYRTHNCNELQISDVETEVKLSGWVHRRRDHGNLVFIDLRDHYGITQIVFTDQNPQLMEDASRLRYESVITVRGTVVA
RSEDTINNTLPTGHVEVLAVEFSVESAADTLPFVINTEKDAPEESRFKHRFLDLRREKLHNNIILRSQIISHIRHLMTAS
GFTEFQTPILTASSPEGARDFLVPSRMHPGKFYALPQAPQQFKQLLMVSGFDRYFQIAPCFRDEDARADRSPGEFYQLDV
EMSFVTQEDVFSTIEPVMYDLFTKFTDKKVSETPFVCIPYNESMLKYGSDKPDLRNPIIIADVTEIFRDSDFTIFRENIK
KGSIVRAIPAPKAAALPRSFFDKMIEFAVSEGAGGLGYIQFSETGEAKGPVVKFLSPQQLESLKATASISNGDAVFFASD
KKEKAAKLAGKVRIRLGEELDLLEKDCFKFCWITDFPFYELNEETGKIDFSHNPFSMPQGGIDALEQAKTTEELLELTAY
QYDIVCNGIELSSGAIRNHKPEIMYKAFSIAGYSEEEVDKRFGGMIRAFKFGAPPHGGIAPGIDRIVMLLAEATNIREII
AFPLNQQAEDLLMNAPSYVEDKALKELSIMLSPSARKNAEKE

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI40789249, Length=612, Percent_Identity=35.2941176470588, Blast_Score=349, Evalue=4e-96,
Organism=Homo sapiens, GI45439306, Length=291, Percent_Identity=24.3986254295533, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI5031815, Length=268, Percent_Identity=26.1194029850746, Blast_Score=76, Evalue=8e-14,
Organism=Homo sapiens, GI194272210, Length=268, Percent_Identity=26.1194029850746, Blast_Score=76, Evalue=1e-13,
Organism=Homo sapiens, GI226491502, Length=263, Percent_Identity=25.0950570342205, Blast_Score=76, Evalue=1e-13,
Organism=Escherichia coli, GI1788173, Length=602, Percent_Identity=42.1926910299003, Blast_Score=468, Evalue=1e-133,
Organism=Escherichia coli, GI1790571, Length=283, Percent_Identity=26.5017667844523, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1789256, Length=302, Percent_Identity=26.4900662251656, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI32566633, Length=610, Percent_Identity=33.6065573770492, Blast_Score=304, Evalue=8e-83,
Organism=Caenorhabditis elegans, GI17551876, Length=276, Percent_Identity=28.6231884057971, Blast_Score=96, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI71984122, Length=297, Percent_Identity=25.5892255892256, Blast_Score=80, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6325153, Length=648, Percent_Identity=29.7839506172839, Blast_Score=220, Evalue=5e-58,
Organism=Saccharomyces cerevisiae, GI6323011, Length=289, Percent_Identity=26.2975778546713, Blast_Score=87, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6321807, Length=251, Percent_Identity=25.0996015936255, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24584738, Length=597, Percent_Identity=36.6834170854271, Blast_Score=340, Evalue=2e-93,
Organism=Drosophila melanogaster, GI17136276, Length=310, Percent_Identity=26.1290322580645, Blast_Score=93, Evalue=7e-19,
Organism=Drosophila melanogaster, GI19921528, Length=234, Percent_Identity=28.2051282051282, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24640849, Length=261, Percent_Identity=26.4367816091954, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24640851, Length=261, Percent_Identity=26.4367816091954, Blast_Score=70, Evalue=4e-12,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYD_RICAE (C3PMI3)

Other databases:

- EMBL:   CP001612
- RefSeq:   YP_002844886.1
- GeneID:   7815184
- GenomeReviews:   CP001612_GR
- KEGG:   raf:RAF_ORF0176
- OMA:   YQLDVEM
- ProtClustDB:   PRK00476
- GO:   GO:0005737
- HAMAP:   MF_00044_B
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF5
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00459

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 68030; Mature: 68030

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKYRTHNCNELQISDVETEVKLSGWVHRRRDHGNLVFIDLRDHYGITQIVFTDQNPQLM
CCCCCCCCCCEEEEECCCEEEEEHHHHHEECCCCCEEEEEECCCCCEEEEEEECCCCHHH
EDASRLRYESVITVRGTVVARSEDTINNTLPTGHVEVLAVEFSVESAADTLPFVINTEKD
HHHHHHHHHEEEEEEEEEEECCCCCCCCCCCCCCEEEEEEEEEHHCCCCCCCEEEECCCC
APEESRFKHRFLDLRREKLHNNIILRSQIISHIRHLMTASGFTEFQTPILTASSPEGARD
CCHHHHHHHHHHHHHHHHHHCCEEHHHHHHHHHHHHHHHCCCCHHCCCEEECCCCCCCHH
FLVPSRMHPGKFYALPQAPQQFKQLLMVSGFDRYFQIAPCFRDEDARADRSPGEFYQLDV
CCCCCCCCCCEEEECCCCHHHHHHHHHHCCCCHHEEECCCCCCCCCCCCCCCCCEEEEEE
EMSFVTQEDVFSTIEPVMYDLFTKFTDKKVSETPFVCIPYNESMLKYGSDKPDLRNPIII
EEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHCCCCCCCCCCCEEE
ADVTEIFRDSDFTIFRENIKKGSIVRAIPAPKAAALPRSFFDKMIEFAVSEGAGGLGYIQ
EEHHHHHCCCCCEEECCCCCCCCEEEECCCCCHHHCCHHHHHHHHHHHHHCCCCCEEEEE
FSETGEAKGPVVKFLSPQQLESLKATASISNGDAVFFASDKKEKAAKLAGKVRIRLGEEL
ECCCCCCCCCEEECCCHHHHHHHHHHEECCCCCEEEEECCCHHHHHHHHCEEEEEECCCH
DLLEKDCFKFCWITDFPFYELNEETGKIDFSHNPFSMPQGGIDALEQAKTTEELLELTAY
HHHHHHHHHEEEECCCCCEECCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
QYDIVCNGIELSSGAIRNHKPEIMYKAFSIAGYSEEEVDKRFGGMIRAFKFGAPPHGGIA
EEEEEECCEEECCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHEEHCCCCCCCCCCC
PGIDRIVMLLAEATNIREIIAFPLNQQAEDLLMNAPSYVEDKALKELSIMLSPSARKNAE
CCHHHHHHHHHHCCCCHHHEECCCCCCHHHHHHCCCCHHHHHHHHHHHEEECCCCCCCCC
KE
CC
>Mature Secondary Structure
MHKYRTHNCNELQISDVETEVKLSGWVHRRRDHGNLVFIDLRDHYGITQIVFTDQNPQLM
CCCCCCCCCCEEEEECCCEEEEEHHHHHEECCCCCEEEEEECCCCCEEEEEEECCCCHHH
EDASRLRYESVITVRGTVVARSEDTINNTLPTGHVEVLAVEFSVESAADTLPFVINTEKD
HHHHHHHHHEEEEEEEEEEECCCCCCCCCCCCCCEEEEEEEEEHHCCCCCCCEEEECCCC
APEESRFKHRFLDLRREKLHNNIILRSQIISHIRHLMTASGFTEFQTPILTASSPEGARD
CCHHHHHHHHHHHHHHHHHHCCEEHHHHHHHHHHHHHHHCCCCHHCCCEEECCCCCCCHH
FLVPSRMHPGKFYALPQAPQQFKQLLMVSGFDRYFQIAPCFRDEDARADRSPGEFYQLDV
CCCCCCCCCCEEEECCCCHHHHHHHHHHCCCCHHEEECCCCCCCCCCCCCCCCCEEEEEE
EMSFVTQEDVFSTIEPVMYDLFTKFTDKKVSETPFVCIPYNESMLKYGSDKPDLRNPIII
EEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHCCCCCCCCCCCEEE
ADVTEIFRDSDFTIFRENIKKGSIVRAIPAPKAAALPRSFFDKMIEFAVSEGAGGLGYIQ
EEHHHHHCCCCCEEECCCCCCCCEEEECCCCCHHHCCHHHHHHHHHHHHHCCCCCEEEEE
FSETGEAKGPVVKFLSPQQLESLKATASISNGDAVFFASDKKEKAAKLAGKVRIRLGEEL
ECCCCCCCCCEEECCCHHHHHHHHHHEECCCCCEEEEECCCHHHHHHHHCEEEEEECCCH
DLLEKDCFKFCWITDFPFYELNEETGKIDFSHNPFSMPQGGIDALEQAKTTEELLELTAY
HHHHHHHHHEEEECCCCCEECCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
QYDIVCNGIELSSGAIRNHKPEIMYKAFSIAGYSEEEVDKRFGGMIRAFKFGAPPHGGIA
EEEEEECCEEECCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHEEHCCCCCCCCCCC
PGIDRIVMLLAEATNIREIIAFPLNQQAEDLLMNAPSYVEDKALKELSIMLSPSARKNAE
CCHHHHHHHHHHCCCCHHHEECCCCCCHHHHHHCCCCHHHHHHHHHHHEEECCCCCCCCC
KE
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA