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The map label for this gene is tsf

Identifier: 229586325

GI number: 229586325

Start: 114014

End: 114943

Strand: Direct

Name: tsf

Synonym: RAF_ORF0108

Alternate gene names: 229586325

Gene position: 114014-114943 (Clockwise)

Preceding gene: 229586324

Following gene: 229586326

Centisome position: 8.92

GC content: 35.27

Gene sequence:

>930_bases
ATGAGTGAAATAAATATAAGTGCTGTAGCAGTTAAAGAATTAAGAGAAAAAACCGGTGCAGGTATGATGGATTGCAAAAA
AGCATTAATAGAAACCAGCGGTAATTTTGAAGAAGCCATTGATTTTCTTCGTAAGAAAGGTTTAGCTGCTGCTGCAAAGA
AAGCCGGACGCATTGCTTCTGAAGGTTTAACGGCTGCAAAAGTTGATGGGCTTACCGGAGTGGTAATTGAAGTAAATTCC
GAAACGGATTTTGTTGCTAGAAATGAGCAGTTTCAGGATCTAGTTAAGGATATTGCAAATCTTGCAGTAATTGCAAAAAC
TATAGACACGCTAAAAACATTTAAAATGCAAAGCGGTAAATCAGTTGAAGAAGAAATTATAGAAAATATCGCTACAATCG
GTGAAAATTTAACATTACGTCGTATGGATATATTAGAGATATCTGAAGGAGCAATCGGTTCGTACGTGCATAATGAAGTT
GTACCGAATTTAGGAAAAATTTCCGTATTAGTAGGTCTTGCGTCTAACGCAAAAGATAAAGCAAAACTAGAAGCTTTAGC
TAAACAAATTGCTGTTCATGTAGCAGGAAATAACCCGCAAAGTATAGATGATTCAAGCTTGGATCAAGCACTCGTAGAGC
GTGAGAGAAAAGTATTTTTTGAAAAATCTAAAGAAGAAGGAAAGCCTGATAATATTATAGCAAAAATGGTAGAAGGTAGA
ATACGTAAATTCTTCTCGGAAGTTGTATTACTTCAGCAAAATTTCTTATTTGAACCTAAACTTACCGTTGCGGAAGTAAT
TAAAAATGCTGAAAAAGAACTCGGTGCAGAAATTAAAATTGCCAAATTTATTAGATACGAACTTGGTGAAGGTATAGAAC
ACGAAGAAAAAAACTTTGCCGATGAAGTAGCTGCCATTACGCAAGGTTAA

Upstream 100 bases:

>100_bases
TTGTTTTTATGGACCCCAGGAGCAAGCCATGGGGTGACACCGGGGTCGTTCTTCGAGCTACAACCCAAGCCAAATATAAT
AATTAAGGAGAAAGTAAGGT

Downstream 100 bases:

>100_bases
TGGGCTTGTTTCATAGCTTCAATGTCATTCCCGCGTGGATACCAAATCGTCATTGTGAGCATGCGTTGTTGTGCAGACAC
AATGCTATCCTGTCATCCCG

Product: elongation factor Ts

Products: NA

Alternate protein names: EF-Ts

Number of amino acids: Translated: 309; Mature: 308

Protein sequence:

>309_residues
MSEINISAVAVKELREKTGAGMMDCKKALIETSGNFEEAIDFLRKKGLAAAAKKAGRIASEGLTAAKVDGLTGVVIEVNS
ETDFVARNEQFQDLVKDIANLAVIAKTIDTLKTFKMQSGKSVEEEIIENIATIGENLTLRRMDILEISEGAIGSYVHNEV
VPNLGKISVLVGLASNAKDKAKLEALAKQIAVHVAGNNPQSIDDSSLDQALVERERKVFFEKSKEEGKPDNIIAKMVEGR
IRKFFSEVVLLQQNFLFEPKLTVAEVIKNAEKELGAEIKIAKFIRYELGEGIEHEEKNFADEVAAITQG

Sequences:

>Translated_309_residues
MSEINISAVAVKELREKTGAGMMDCKKALIETSGNFEEAIDFLRKKGLAAAAKKAGRIASEGLTAAKVDGLTGVVIEVNS
ETDFVARNEQFQDLVKDIANLAVIAKTIDTLKTFKMQSGKSVEEEIIENIATIGENLTLRRMDILEISEGAIGSYVHNEV
VPNLGKISVLVGLASNAKDKAKLEALAKQIAVHVAGNNPQSIDDSSLDQALVERERKVFFEKSKEEGKPDNIIAKMVEGR
IRKFFSEVVLLQQNFLFEPKLTVAEVIKNAEKELGAEIKIAKFIRYELGEGIEHEEKNFADEVAAITQG
>Mature_308_residues
SEINISAVAVKELREKTGAGMMDCKKALIETSGNFEEAIDFLRKKGLAAAAKKAGRIASEGLTAAKVDGLTGVVIEVNSE
TDFVARNEQFQDLVKDIANLAVIAKTIDTLKTFKMQSGKSVEEEIIENIATIGENLTLRRMDILEISEGAIGSYVHNEVV
PNLGKISVLVGLASNAKDKAKLEALAKQIAVHVAGNNPQSIDDSSLDQALVERERKVFFEKSKEEGKPDNIIAKMVEGRI
RKFFSEVVLLQQNFLFEPKLTVAEVIKNAEKELGAEIKIAKFIRYELGEGIEHEEKNFADEVAAITQG

Specific function: Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome

COG id: COG0264

COG function: function code J; Translation elongation factor Ts

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EF-Ts family

Homologues:

Organism=Homo sapiens, GI171846268, Length=307, Percent_Identity=28.3387622149837, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI291084500, Length=323, Percent_Identity=27.2445820433437, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI291084498, Length=91, Percent_Identity=45.0549450549451, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI291084502, Length=91, Percent_Identity=45.0549450549451, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1786366, Length=305, Percent_Identity=43.6065573770492, Blast_Score=213, Evalue=9e-57,
Organism=Caenorhabditis elegans, GI17561440, Length=315, Percent_Identity=30.1587301587302, Blast_Score=113, Evalue=1e-25,
Organism=Drosophila melanogaster, GI19921466, Length=312, Percent_Identity=24.3589743589744, Blast_Score=80, Evalue=3e-15,

Paralogues:

None

Copy number: 2670 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1100 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 4173 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 14563 Molecules/Cell In: Growth Phase, Gluco

Swissprot (AC and ID): EFTS_RICAE (C3PMC3)

Other databases:

- EMBL:   CP001612
- RefSeq:   YP_002844826.1
- GeneID:   7815360
- GenomeReviews:   CP001612_GR
- KEGG:   raf:RAF_ORF0108
- OMA:   YLHGTRI
- ProtClustDB:   PRK09377
- GO:   GO:0005737
- HAMAP:   MF_00050
- InterPro:   IPR001816
- InterPro:   IPR014039
- InterPro:   IPR018101
- InterPro:   IPR009060
- InterPro:   IPR000449
- Gene3D:   G3DSA:3.30.479.20
- PANTHER:   PTHR11741
- TIGRFAMs:   TIGR00116

Pfam domain/function: PF00889 EF_TS; PF00627 UBA; SSF54713 EF_TS; SSF46934 UBA_like

EC number: NA

Molecular weight: Translated: 33775; Mature: 33644

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS01126 EF_TS_1; PS01127 EF_TS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEINISAVAVKELREKTGAGMMDCKKALIETSGNFEEAIDFLRKKGLAAAAKKAGRIAS
CCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHCCHHH
EGLTAAKVDGLTGVVIEVNSETDFVARNEQFQDLVKDIANLAVIAKTIDTLKTFKMQSGK
CCCCHHHHCCCEEEEEEECCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SVEEEIIENIATIGENLTLRRMDILEISEGAIGSYVHNEVVPNLGKISVLVGLASNAKDK
CHHHHHHHHHHHHCCCCEEEHHHHHHHCCCCHHHHHHHHHCCCCCHHHHEEEECCCCHHH
AKLEALAKQIAVHVAGNNPQSIDDSSLDQALVERERKVFFEKSKEEGKPDNIIAKMVEGR
HHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
IRKFFSEVVLLQQNFLFEPKLTVAEVIKNAEKELGAEIKIAKFIRYELGEGIEHEEKNFA
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHH
DEVAAITQG
HHHHHHCCC
>Mature Secondary Structure 
SEINISAVAVKELREKTGAGMMDCKKALIETSGNFEEAIDFLRKKGLAAAAKKAGRIAS
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHCCHHH
EGLTAAKVDGLTGVVIEVNSETDFVARNEQFQDLVKDIANLAVIAKTIDTLKTFKMQSGK
CCCCHHHHCCCEEEEEEECCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SVEEEIIENIATIGENLTLRRMDILEISEGAIGSYVHNEVVPNLGKISVLVGLASNAKDK
CHHHHHHHHHHHHCCCCEEEHHHHHHHCCCCHHHHHHHHHCCCCCHHHHEEEECCCCHHH
AKLEALAKQIAVHVAGNNPQSIDDSSLDQALVERERKVFFEKSKEEGKPDNIIAKMVEGR
HHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
IRKFFSEVVLLQQNFLFEPKLTVAEVIKNAEKELGAEIKIAKFIRYELGEGIEHEEKNFA
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHH
DEVAAITQG
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA