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The map label for this gene is kdsA [H]

Identifier: 229586302

GI number: 229586302

Start: 83381

End: 84205

Strand: Direct

Name: kdsA [H]

Synonym: RAF_ORF0085

Alternate gene names: 229586302

Gene position: 83381-84205 (Clockwise)

Preceding gene: 229586301

Following gene: 229586303

Centisome position: 6.52

GC content: 35.39

Gene sequence:

>825_bases
ATGCAAAAAGTAGTAAAACTTAACAATATCAAAATAGGAAATGATTTACCGTTTGTTCTAATTACAGGGCCTTGTCAGAT
TGAAGGAAAAGATCATGCGTTGTTTATGGCAGAAAAACTTGTTAAATTAACTAGCAAGCTTGAGATTCCATTTATTTATA
AATCTTCTTTTGATAAAGCTAATAGAACTTCCGTACATGGGATTAGAGGAGTTGGTATTGAGAAAGGTTTAGAAATTTTA
TCAAAAGTAAAATCTGAATTCGATTGCCCTATTGTTACTGATGTTCATTCAGAGAGTCAATGTACTGAAACAGCCGAAGT
AGCAGATATTTTACAAATTCCGGCATTTTTATGTAGACAAACCGACTTGCTTCAAGCTGCAGCAAAAACAGGTAAAATAG
TAAAAGTAAAAAAAGGACAGTTTCTAGCTCCGTGGGATATGAAAAATGTACAAACAAAACTAGAAGCTTTTGGAGTAAAG
GATATATTATTTACAGAGCGAGGAGCATGTTTTGGCTATAATAACTTGGTATCTGATATGCGTAGTCTTGCAATAATGGC
AGAGTTAAATGTACCTGTAGTTTTTGATGCCACACATTCCGTTCAGCAACCGGGCGGGCTAGGAGGCAGTACCGGCGGAG
AAAGAAAATATGTAGAGTTACTTGCAAAGGCAGCAACTTCTGTAGGTATCGCCGGTATGTATATGGAAGTACATCAAGAC
CCCGATAACGCTCCAAGCGATGGTCCATGTATGATGAAGTTGGATAATCTAGAGTCTATTCTTATCAAACTAAAAAAATA
TGATAAAATTACAAAAGAAAAATAA

Upstream 100 bases:

>100_bases
CACTAATAATTAATAATGACTTAATAGAAAATGAATCAGTACCTGATATAACAGAGAAACACTAATCCGTGCCTCGTTCA
ATTAATTAAATCTAAGAATT

Downstream 100 bases:

>100_bases
CTTCAAATTTTACCTTTCAAAATATATATAAATATCCCCTATTAATTCAATTTCAAAATAGAAGTAATAATTTTTTACAA
TATAAAAAAATAGAACTTTC

Product: 2-dehydro-3-deoxyphosphooctonate aldolase

Products: NA

Alternate protein names: 3-deoxy-D-manno-octulosonic acid 8-phosphate synthase; KDO-8-phosphate synthase; KDO 8-P synthase; KDOPS; Phospho-2-dehydro-3-deoxyoctonate aldolase [H]

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MQKVVKLNNIKIGNDLPFVLITGPCQIEGKDHALFMAEKLVKLTSKLEIPFIYKSSFDKANRTSVHGIRGVGIEKGLEIL
SKVKSEFDCPIVTDVHSESQCTETAEVADILQIPAFLCRQTDLLQAAAKTGKIVKVKKGQFLAPWDMKNVQTKLEAFGVK
DILFTERGACFGYNNLVSDMRSLAIMAELNVPVVFDATHSVQQPGGLGGSTGGERKYVELLAKAATSVGIAGMYMEVHQD
PDNAPSDGPCMMKLDNLESILIKLKKYDKITKEK

Sequences:

>Translated_274_residues
MQKVVKLNNIKIGNDLPFVLITGPCQIEGKDHALFMAEKLVKLTSKLEIPFIYKSSFDKANRTSVHGIRGVGIEKGLEIL
SKVKSEFDCPIVTDVHSESQCTETAEVADILQIPAFLCRQTDLLQAAAKTGKIVKVKKGQFLAPWDMKNVQTKLEAFGVK
DILFTERGACFGYNNLVSDMRSLAIMAELNVPVVFDATHSVQQPGGLGGSTGGERKYVELLAKAATSVGIAGMYMEVHQD
PDNAPSDGPCMMKLDNLESILIKLKKYDKITKEK
>Mature_274_residues
MQKVVKLNNIKIGNDLPFVLITGPCQIEGKDHALFMAEKLVKLTSKLEIPFIYKSSFDKANRTSVHGIRGVGIEKGLEIL
SKVKSEFDCPIVTDVHSESQCTETAEVADILQIPAFLCRQTDLLQAAAKTGKIVKVKKGQFLAPWDMKNVQTKLEAFGVK
DILFTERGACFGYNNLVSDMRSLAIMAELNVPVVFDATHSVQQPGGLGGSTGGERKYVELLAKAATSVGIAGMYMEVHQD
PDNAPSDGPCMMKLDNLESILIKLKKYDKITKEK

Specific function: Synthesis Of Kdo 8-P Which Is Required For Lipid A Maturation And Cellular Growth. [C]

COG id: COG2877

COG function: function code M; 3-deoxy-D-manno-octulosonic acid (KDO) 8-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the kdsA family [H]

Homologues:

Organism=Escherichia coli, GI1787466, Length=273, Percent_Identity=45.4212454212454, Blast_Score=259, Evalue=1e-70,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006218
- InterPro:   IPR006269 [H]

Pfam domain/function: PF00793 DAHP_synth_1 [H]

EC number: =2.5.1.55 [H]

Molecular weight: Translated: 30048; Mature: 30048

Theoretical pI: Translated: 8.12; Mature: 8.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKVVKLNNIKIGNDLPFVLITGPCQIEGKDHALFMAEKLVKLTSKLEIPFIYKSSFDKA
CCCEEEECCEEECCCCCEEEEECCEEECCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCC
NRTSVHGIRGVGIEKGLEILSKVKSEFDCPIVTDVHSESQCTETAEVADILQIPAFLCRQ
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
TDLLQAAAKTGKIVKVKKGQFLAPWDMKNVQTKLEAFGVKDILFTERGACFGYNNLVSDM
HHHHHHHHHCCCEEEEECCCEECCCCHHHHHHHHHHCCCHHHEEECCCCCCCHHHHHHHH
RSLAIMAELNVPVVFDATHSVQQPGGLGGSTGGERKYVELLAKAATSVGIAGMYMEVHQD
HHHEEEEECCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHEEEEEEECC
PDNAPSDGPCMMKLDNLESILIKLKKYDKITKEK
CCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MQKVVKLNNIKIGNDLPFVLITGPCQIEGKDHALFMAEKLVKLTSKLEIPFIYKSSFDKA
CCCEEEECCEEECCCCCEEEEECCEEECCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCC
NRTSVHGIRGVGIEKGLEILSKVKSEFDCPIVTDVHSESQCTETAEVADILQIPAFLCRQ
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
TDLLQAAAKTGKIVKVKKGQFLAPWDMKNVQTKLEAFGVKDILFTERGACFGYNNLVSDM
HHHHHHHHHCCCEEEEECCCEECCCCHHHHHHHHHHCCCHHHEEECCCCCCCHHHHHHHH
RSLAIMAELNVPVVFDATHSVQQPGGLGGSTGGERKYVELLAKAATSVGIAGMYMEVHQD
HHHEEEEECCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHEEEEEEECC
PDNAPSDGPCMMKLDNLESILIKLKKYDKITKEK
CCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA