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The map label for this gene is dus [H]

Identifier: 229586241

GI number: 229586241

Start: 9920

End: 10939

Strand: Direct

Name: dus [H]

Synonym: RAF_ORF0011

Alternate gene names: 229586241

Gene position: 9920-10939 (Clockwise)

Preceding gene: 229586235

Following gene: 229586242

Centisome position: 0.78

GC content: 38.53

Gene sequence:

>1020_bases
GTGTTTATTTTTATAACGGTTTTTATAATCAAAATCGGCAATATTGAGCTTTCTTCAAATATTATACTAGCTCCAATGTC
AGGTGTTACGGATTTGGAATTTAGAAGATTAGTGAAAAGATTTGGTGCAGGGCTTGTAGTTTCTGAAATGGTAGCAAGTA
GAGCAATGATTGTGGAATCTAGGCAGTCACTAAAAAAAAGTGCCATTATGCGTGATGATGCAACAAGTGCTTGCGTACAG
CTAGCAGGCTGTGAGCCGAATGTGATAGCAGAAGCTGCTAAAATGAATGAGGGTATGGGAGCAAAAATTATCGATCTTAA
TTTTGGCTGTCCTGCGAAAAAAGTAGTAGGCGGTTATTCAGGCTTGGTTCTAATGAGGGATGAGCAGTTGGCAGCTAAAA
TTTTTGAAGCTACCGTTGAAGCAGTTAAGCTGCCGGTAACTGTTAAAATGCGTATGGGTTGGGATGATAATACGAAAAAC
GCTCCGACTTTAGCCAAAATTGCTGAAAGCTTAGGTGTTCAGATGATTACCGTTCATGGTAGAACTAGATGCCAGTTTTA
TTCCGGTAATGTCGATTGGGATTTTATACGATCAGTTAAAGAATCGGTAAAGATTCAGGTTATTGCTAATGGTGATATTA
CTAATTTTGCTAAGGCAAAAGAAGCCCTACAAAAATCTGGTGCAGACGGTATTATGGTCGGTAGAGGAGCATATGGCAAA
CCTTGGCTTATTTCACAAATTGATCATTATCTTAAAACAGGTGAAGAAAAATCTGCTCCCTCTATAGCAGAGCAGCTAGA
TATCGTAACCGAGCATTATGAGGCAATACTTGATTATTATGGTGAATCTGTGGGCGTACCTATTGCACGTAAGCATATTG
GCTGGTATAGTAGCGGTTTACCGAATTCAGCAGAGTTTAGAGGTGCTGTTAATTTAATGAACGATCCTCTAGCTGTAAAA
GAGAAAATTGCGGAATTTTATACGAGCGTTATGGAGACGAATAAATTAAAAGAATTTTAA

Upstream 100 bases:

>100_bases
GGTGTAAGGAAATATAACTTAAGCTTATAATAGATTAATCGGCTTTTGTCTATTCTTAATTAGTAATATTAGACAAAAAT
ACTTAATTATGATATCATGA

Downstream 100 bases:

>100_bases
GTTTTATTTTTGTAATATTGTTTTTTAGTTTAAGCTATGCAAGTGAAAAAGCAGTAATTATAACTGATTATAAACCGGTA
TTTTTGCCTATAATTGCTGA

Product: Putative dihydrouridine synthase Dus

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MFIFITVFIIKIGNIELSSNIILAPMSGVTDLEFRRLVKRFGAGLVVSEMVASRAMIVESRQSLKKSAIMRDDATSACVQ
LAGCEPNVIAEAAKMNEGMGAKIIDLNFGCPAKKVVGGYSGLVLMRDEQLAAKIFEATVEAVKLPVTVKMRMGWDDNTKN
APTLAKIAESLGVQMITVHGRTRCQFYSGNVDWDFIRSVKESVKIQVIANGDITNFAKAKEALQKSGADGIMVGRGAYGK
PWLISQIDHYLKTGEEKSAPSIAEQLDIVTEHYEAILDYYGESVGVPIARKHIGWYSSGLPNSAEFRGAVNLMNDPLAVK
EKIAEFYTSVMETNKLKEF

Sequences:

>Translated_339_residues
MFIFITVFIIKIGNIELSSNIILAPMSGVTDLEFRRLVKRFGAGLVVSEMVASRAMIVESRQSLKKSAIMRDDATSACVQ
LAGCEPNVIAEAAKMNEGMGAKIIDLNFGCPAKKVVGGYSGLVLMRDEQLAAKIFEATVEAVKLPVTVKMRMGWDDNTKN
APTLAKIAESLGVQMITVHGRTRCQFYSGNVDWDFIRSVKESVKIQVIANGDITNFAKAKEALQKSGADGIMVGRGAYGK
PWLISQIDHYLKTGEEKSAPSIAEQLDIVTEHYEAILDYYGESVGVPIARKHIGWYSSGLPNSAEFRGAVNLMNDPLAVK
EKIAEFYTSVMETNKLKEF
>Mature_339_residues
MFIFITVFIIKIGNIELSSNIILAPMSGVTDLEFRRLVKRFGAGLVVSEMVASRAMIVESRQSLKKSAIMRDDATSACVQ
LAGCEPNVIAEAAKMNEGMGAKIIDLNFGCPAKKVVGGYSGLVLMRDEQLAAKIFEATVEAVKLPVTVKMRMGWDDNTKN
APTLAKIAESLGVQMITVHGRTRCQFYSGNVDWDFIRSVKESVKIQVIANGDITNFAKAKEALQKSGADGIMVGRGAYGK
PWLISQIDHYLKTGEEKSAPSIAEQLDIVTEHYEAILDYYGESVGVPIARKHIGWYSSGLPNSAEFRGAVNLMNDPLAVK
EKIAEFYTSVMETNKLKEF

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=289, Percent_Identity=29.0657439446367, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI239788462, Length=290, Percent_Identity=28.9655172413793, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI40807366, Length=257, Percent_Identity=29.9610894941634, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI31742496, Length=253, Percent_Identity=29.6442687747036, Blast_Score=101, Evalue=9e-22,
Organism=Homo sapiens, GI8923374, Length=241, Percent_Identity=26.5560165975104, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1789660, Length=306, Percent_Identity=41.5032679738562, Blast_Score=255, Evalue=3e-69,
Organism=Escherichia coli, GI1788462, Length=234, Percent_Identity=34.6153846153846, Blast_Score=121, Evalue=8e-29,
Organism=Escherichia coli, GI145693211, Length=309, Percent_Identity=27.1844660194175, Blast_Score=99, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17543114, Length=286, Percent_Identity=29.020979020979, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI25144369, Length=227, Percent_Identity=30.8370044052863, Blast_Score=95, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17507177, Length=236, Percent_Identity=27.5423728813559, Blast_Score=90, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323433, Length=306, Percent_Identity=26.4705882352941, Blast_Score=100, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6323560, Length=305, Percent_Identity=26.2295081967213, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6323437, Length=231, Percent_Identity=26.4069264069264, Blast_Score=73, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24585320, Length=297, Percent_Identity=29.2929292929293, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24580595, Length=233, Percent_Identity=29.6137339055794, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI19920448, Length=233, Percent_Identity=29.6137339055794, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI19921524, Length=228, Percent_Identity=28.9473684210526, Blast_Score=95, Evalue=8e-20,
Organism=Drosophila melanogaster, GI45549423, Length=241, Percent_Identity=24.4813278008299, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 37144; Mature: 37144

Theoretical pI: Translated: 7.94; Mature: 7.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFIFITVFIIKIGNIELSSNIILAPMSGVTDLEFRRLVKRFGAGLVVSEMVASRAMIVES
CEEEEEEEEHHCCCEEECCCEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
RQSLKKSAIMRDDATSACVQLAGCEPNVIAEAAKMNEGMGAKIIDLNFGCPAKKVVGGYS
HHHHHHHHHHCCCHHHHHHEECCCCCHHHHHHHHHCCCCCCEEEEECCCCCHHHHHCCCC
GLVLMRDEQLAAKIFEATVEAVKLPVTVKMRMGWDDNTKNAPTLAKIAESLGVQMITVHG
CEEEEECHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHCCEEEEEEEC
RTRCQFYSGNVDWDFIRSVKESVKIQVIANGDITNFAKAKEALQKSGADGIMVGRGAYGK
CEEEEEEECCCCHHHHHHHHHCEEEEEEECCCCHHHHHHHHHHHHCCCCCEEECCCCCCC
PWLISQIDHYLKTGEEKSAPSIAEQLDIVTEHYEAILDYYGESVGVPIARKHIGWYSSGL
CHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCHHCCCC
PNSAEFRGAVNLMNDPLAVKEKIAEFYTSVMETNKLKEF
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFIFITVFIIKIGNIELSSNIILAPMSGVTDLEFRRLVKRFGAGLVVSEMVASRAMIVES
CEEEEEEEEHHCCCEEECCCEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
RQSLKKSAIMRDDATSACVQLAGCEPNVIAEAAKMNEGMGAKIIDLNFGCPAKKVVGGYS
HHHHHHHHHHCCCHHHHHHEECCCCCHHHHHHHHHCCCCCCEEEEECCCCCHHHHHCCCC
GLVLMRDEQLAAKIFEATVEAVKLPVTVKMRMGWDDNTKNAPTLAKIAESLGVQMITVHG
CEEEEECHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHCCEEEEEEEC
RTRCQFYSGNVDWDFIRSVKESVKIQVIANGDITNFAKAKEALQKSGADGIMVGRGAYGK
CEEEEEEECCCCHHHHHHHHHCEEEEEEECCCCHHHHHHHHHHHHCCCCCEEECCCCCCC
PWLISQIDHYLKTGEEKSAPSIAEQLDIVTEHYEAILDYYGESVGVPIARKHIGWYSSGL
CHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCHHCCCC
PNSAEFRGAVNLMNDPLAVKEKIAEFYTSVMETNKLKEF
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]