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The map label for this gene is rfbE [H]

Identifier: 229586233

GI number: 229586233

Start: 1368

End: 2117

Strand: Direct

Name: rfbE [H]

Synonym: RAF_ORF0003

Alternate gene names: 229586233

Gene position: 1368-2117 (Clockwise)

Preceding gene: 229586232

Following gene: 229586234

Centisome position: 0.11

GC content: 29.33

Gene sequence:

>750_bases
ATGTCACTTAGCTATTCAAAAGTATTTATAGAGATAACTAATGGTTATGTTGAAGGTATTGATACTCATAAAAGAGCTCA
AGGTTTAAAGCATTTCTTCTTGAAAAAAGGAGTCTCTCTTTCTCCAAATATACCTATATTAAACAATATTAATTTTTCTT
GTTATGAGGGAGAAAAAATAGCTTTTATAGGGAGTAACGGTTCGGGAAAAAGTTCGTTTCTAAAGTTAATCGCCGGAATA
TATCCGTTAAAATCCGGTACAGTCAAAGTTCATGGAGATATTGCGGCAATTATAGATATGGGAGTTGGTTTTGAGTCTGA
GCAAACTGGTCGTGAAAATATAAAAATGTTAATGCTATATAATAATATGCTGGATAAATATAGCAAAAAAATTGAAAAAG
AAATTATAGATTTTTCAGAACTTGGTAGTAAAATTGATTTACCGATAAAAATTTATAGCTCCGGTATGTTATCACGTCTT
GCTTTTTCTGTATCGATATTTCAAAATCCGCAGATTCTATTACTTGATGAAGTTTTTGCGGCAGGCGATAGCTATTTTAT
AGAAAAATCTCTTAGTTTAATGAAGAATAAATTTAAAAATACTCCTATTTCAATAATAGTAAGCCATCAAGAAGAAATTA
TAAAAGATAATTGCAATAGATGTATTTTATTAAAAGACGGCAATATTATAGGTGATGGTACACCATCGGAAATGTTTAAA
ATCTATAACCAACAACAAGGAAATTCATAA

Upstream 100 bases:

>100_bases
ATGTTATTTAAAAATGGTGAACAAAAAGATACTAAAATAGGTTTGCAACAAAAAAATTCTCTTTTAGATTGGATTAATAA
ATCTATTTAATATTTATTTT

Downstream 100 bases:

>100_bases
ATGATAAAGTATTTTTTTTCTAAAAAATACTGGCGAGCAATAGCATTACTAGTTAAAGCTTCTATAATTAGGCAAAATAA
AGATTCTTTTTTAGGTTCTT

Product: O-antigen export system ATP-binding protein RfbE

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 249; Mature: 248

Protein sequence:

>249_residues
MSLSYSKVFIEITNGYVEGIDTHKRAQGLKHFFLKKGVSLSPNIPILNNINFSCYEGEKIAFIGSNGSGKSSFLKLIAGI
YPLKSGTVKVHGDIAAIIDMGVGFESEQTGRENIKMLMLYNNMLDKYSKKIEKEIIDFSELGSKIDLPIKIYSSGMLSRL
AFSVSIFQNPQILLLDEVFAAGDSYFIEKSLSLMKNKFKNTPISIIVSHQEEIIKDNCNRCILLKDGNIIGDGTPSEMFK
IYNQQQGNS

Sequences:

>Translated_249_residues
MSLSYSKVFIEITNGYVEGIDTHKRAQGLKHFFLKKGVSLSPNIPILNNINFSCYEGEKIAFIGSNGSGKSSFLKLIAGI
YPLKSGTVKVHGDIAAIIDMGVGFESEQTGRENIKMLMLYNNMLDKYSKKIEKEIIDFSELGSKIDLPIKIYSSGMLSRL
AFSVSIFQNPQILLLDEVFAAGDSYFIEKSLSLMKNKFKNTPISIIVSHQEEIIKDNCNRCILLKDGNIIGDGTPSEMFK
IYNQQQGNS
>Mature_248_residues
SLSYSKVFIEITNGYVEGIDTHKRAQGLKHFFLKKGVSLSPNIPILNNINFSCYEGEKIAFIGSNGSGKSSFLKLIAGIY
PLKSGTVKVHGDIAAIIDMGVGFESEQTGRENIKMLMLYNNMLDKYSKKIEKEIIDFSELGSKIDLPIKIYSSGMLSRLA
FSVSIFQNPQILLLDEVFAAGDSYFIEKSLSLMKNKFKNTPISIIVSHQEEIIKDNCNRCILLKDGNIIGDGTPSEMFKI
YNQQQGNS

Specific function: May form an ATP-driven O-antigen export apparatus, in association with RfbA [H]

COG id: COG1134

COG function: function code GM; ABC-type polysaccharide/polyol phosphate transport system, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI157502201, Length=205, Percent_Identity=29.2682926829268, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI157502203, Length=205, Percent_Identity=29.2682926829268, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI89111135, Length=204, Percent_Identity=25.9803921568627, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI153792144, Length=197, Percent_Identity=21.8274111675127, Blast_Score=64, Evalue=1e-10,
Organism=Escherichia coli, GI1788761, Length=217, Percent_Identity=26.2672811059908, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI48995001, Length=247, Percent_Identity=24.6963562753036, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI48994943, Length=206, Percent_Identity=25.7281553398058, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI1788210, Length=210, Percent_Identity=26.6666666666667, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786398, Length=218, Percent_Identity=25.2293577981651, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787105, Length=204, Percent_Identity=23.5294117647059, Blast_Score=67, Evalue=8e-13,
Organism=Escherichia coli, GI1789032, Length=236, Percent_Identity=27.1186440677966, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787792, Length=207, Percent_Identity=26.5700483091787, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787041, Length=194, Percent_Identity=28.8659793814433, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI25146777, Length=245, Percent_Identity=27.3469387755102, Blast_Score=77, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI212646699, Length=233, Percent_Identity=28.3261802575107, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17551702, Length=210, Percent_Identity=28.0952380952381, Blast_Score=68, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17569081, Length=214, Percent_Identity=29.4392523364486, Blast_Score=68, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17551700, Length=210, Percent_Identity=28.0952380952381, Blast_Score=68, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6322640, Length=232, Percent_Identity=23.7068965517241, Blast_Score=68, Evalue=1e-12,
Organism=Drosophila melanogaster, GI28574259, Length=202, Percent_Identity=25.7425742574257, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24582014, Length=230, Percent_Identity=25.6521739130435, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24582012, Length=230, Percent_Identity=25.6521739130435, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI45552437, Length=206, Percent_Identity=26.2135922330097, Blast_Score=64, Evalue=8e-11,
Organism=Drosophila melanogaster, GI24585557, Length=206, Percent_Identity=26.2135922330097, Blast_Score=64, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: 3.6.3.25 [C]

Molecular weight: Translated: 27731; Mature: 27599

Theoretical pI: Translated: 8.42; Mature: 8.42

Prosite motif: PS00211 ABC_TRANSPORTER_1 ; PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLSYSKVFIEITNGYVEGIDTHKRAQGLKHFFLKKGVSLSPNIPILNNINFSCYEGEKI
CCCCEEEEEEEEECCEECCCCHHHHHHHHHHHHHHCCCCCCCCCCEEECCCEEEECCCEE
AFIGSNGSGKSSFLKLIAGIYPLKSGTVKVHGDIAAIIDMGVGFESEQTGRENIKMLMLY
EEEECCCCCHHHHHHHHHHHCCCCCCCEEEECCEEEEEECCCCCCCCCCCCCCEEEEEHH
NNMLDKYSKKIEKEIIDFSELGSKIDLPIKIYSSGMLSRLAFSVSIFQNPQILLLDEVFA
HHHHHHHHHHHHHHHHCHHHCCCCCCCEEEEECCHHHHHHHHEEEEECCCCEEEEEHHHH
AGDSYFIEKSLSLMKNKFKNTPISIIVSHQEEIIKDNCNRCILLKDGNIIGDGTPSEMFK
CCCHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHCCCCEEEEEECCCEECCCCHHHHHH
IYNQQQGNS
HHHHCCCCC
>Mature Secondary Structure 
SLSYSKVFIEITNGYVEGIDTHKRAQGLKHFFLKKGVSLSPNIPILNNINFSCYEGEKI
CCCEEEEEEEEECCEECCCCHHHHHHHHHHHHHHCCCCCCCCCCEEECCCEEEECCCEE
AFIGSNGSGKSSFLKLIAGIYPLKSGTVKVHGDIAAIIDMGVGFESEQTGRENIKMLMLY
EEEECCCCCHHHHHHHHHHHCCCCCCCEEEECCEEEEEECCCCCCCCCCCCCCEEEEEHH
NNMLDKYSKKIEKEIIDFSELGSKIDLPIKIYSSGMLSRLAFSVSIFQNPQILLLDEVFA
HHHHHHHHHHHHHHHHCHHHCCCCCCCEEEEECCHHHHHHHHEEEEECCCCEEEEEHHHH
AGDSYFIEKSLSLMKNKFKNTPISIIVSHQEEIIKDNCNRCILLKDGNIIGDGTPSEMFK
CCCHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHCCCCEEEEEECCCEECCCCHHHHHH
IYNQQQGNS
HHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8626291 [H]