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The map label for this gene is ppsA [H]

Identifier: 227828719

GI number: 227828719

Start: 2301567

End: 2302505

Strand: Direct

Name: ppsA [H]

Synonym: M1425_2477

Alternate gene names: 227828719

Gene position: 2301567-2302505 (Clockwise)

Preceding gene: 227828718

Following gene: 227828725

Centisome position: 88.22

GC content: 37.38

Gene sequence:

>939_bases
ATGAATTATACATACTTGCTAGAGGAAGTTAGTTTATCCATGGTTTCAATTGTAGGTAGAAAAAGTGCCTACCTAGGCGA
ATTATATAAGATGGGATTCAACGTCCCTAAAGGGTTCATAGTGTCGTCAAGAGGAGTAAATGAGGCGATTAAAGATCTGG
ACGATGAAATAAGAGGAATACTATCTTCTGTTAATCTAAACGATACGACAGATCTCGAAAAGAAAAGTGAAATGATCAAA
AACATGATCATAATGTCGAAATTACCTGAGGAAATGGAAAAAGAAATATACGAGAGATTCTCTCAACTGGGTTCAAAATA
CGTCGCAGTAAGGGCTACAGCCACATCTCCCTTAAGTGGTGCAAGTTTCGCTGGTGAGTATGAAACCGATTTATTTGTTA
CTAAGGAAAATCTTATTCCGAGTATTAAGAGAGTTATTGCGTCGTATTTTAACCCAAGGGCAATAGCTTATAGAATTCTG
ACTCATAATGAAGCTGGAATGGCAATTTTCGTCCAAACAATGATAAACCCGGTTAGTGCAGGGACTGCGTTCTCAATACA
TCCCATAACTGAAGAACCAGACTACGTATTTATAGAGTCATCTTTTGGTCTAGGCGAAAGTGTAACAAGAGGCATGGTCA
CTCCAGATCAATATATAGTGAGCAAGGCTACTAGATCACTGGTAAGTAAAAGGATTTCTGAGAAGGTCATGAAACTAACT
TACGATTTCGAGGACAGAAAGATAAAGAGCGTAGAACTGAGTAAGGAGGAAGCATCAGCTGAAAGCCTAAGTGATAATGA
TGCCATAAGGATTGCAAACATGGTTATAGCTGTAGAAAATATATTTAAGAGAAATATAAATATTGAATGGGCAATGGAAG
ATAAAAAAGTGTACTTATTGGAAGTGAGAGGGATTAGGCGATTATACCCCGAATTTTAA

Upstream 100 bases:

>100_bases
GTTTCAACATTTACACTCATGATGAAAGAATATATTAGATGATAACTTTTTAACATTATTAATAGGTAGTATTTTAAACC
TATCTATCAAAATGTAACTT

Downstream 100 bases:

>100_bases
CCATACCTTAATGCTATGCCTAACCCATACCTTGGATACTTCCAAGAGATGTTAGAATAGGGACAAGCAATCCTACAAGC
ACCACATTCCACACAATTCT

Product: pyruvate, water dikinase

Products: NA

Alternate protein names: PEP synthase; Pyruvate, water dikinase; Mja pepA intein [H]

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MNYTYLLEEVSLSMVSIVGRKSAYLGELYKMGFNVPKGFIVSSRGVNEAIKDLDDEIRGILSSVNLNDTTDLEKKSEMIK
NMIIMSKLPEEMEKEIYERFSQLGSKYVAVRATATSPLSGASFAGEYETDLFVTKENLIPSIKRVIASYFNPRAIAYRIL
THNEAGMAIFVQTMINPVSAGTAFSIHPITEEPDYVFIESSFGLGESVTRGMVTPDQYIVSKATRSLVSKRISEKVMKLT
YDFEDRKIKSVELSKEEASAESLSDNDAIRIANMVIAVENIFKRNINIEWAMEDKKVYLLEVRGIRRLYPEF

Sequences:

>Translated_312_residues
MNYTYLLEEVSLSMVSIVGRKSAYLGELYKMGFNVPKGFIVSSRGVNEAIKDLDDEIRGILSSVNLNDTTDLEKKSEMIK
NMIIMSKLPEEMEKEIYERFSQLGSKYVAVRATATSPLSGASFAGEYETDLFVTKENLIPSIKRVIASYFNPRAIAYRIL
THNEAGMAIFVQTMINPVSAGTAFSIHPITEEPDYVFIESSFGLGESVTRGMVTPDQYIVSKATRSLVSKRISEKVMKLT
YDFEDRKIKSVELSKEEASAESLSDNDAIRIANMVIAVENIFKRNINIEWAMEDKKVYLLEVRGIRRLYPEF
>Mature_312_residues
MNYTYLLEEVSLSMVSIVGRKSAYLGELYKMGFNVPKGFIVSSRGVNEAIKDLDDEIRGILSSVNLNDTTDLEKKSEMIK
NMIIMSKLPEEMEKEIYERFSQLGSKYVAVRATATSPLSGASFAGEYETDLFVTKENLIPSIKRVIASYFNPRAIAYRIL
THNEAGMAIFVQTMINPVSAGTAFSIHPITEEPDYVFIESSFGLGESVTRGMVTPDQYIVSKATRSLVSKRISEKVMKLT
YDFEDRKIKSVELSKEEASAESLSDNDAIRIANMVIAVENIFKRNINIEWAMEDKKVYLLEVRGIRRLYPEF

Specific function: Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DOD-type homing endonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=322, Percent_Identity=30.1242236024845, Blast_Score=134, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17564524, Length=237, Percent_Identity=26.1603375527426, Blast_Score=71, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR003587
- InterPro:   IPR004042
- InterPro:   IPR006141
- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR006319
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.2 [H]

Molecular weight: Translated: 35251; Mature: 35251

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYTYLLEEVSLSMVSIVGRKSAYLGELYKMGFNVPKGFIVSSRGVNEAIKDLDDEIRGI
CCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHH
LSSVNLNDTTDLEKKSEMIKNMIIMSKLPEEMEKEIYERFSQLGSKYVAVRATATSPLSG
HHHCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCCC
ASFAGEYETDLFVTKENLIPSIKRVIASYFNPRAIAYRILTHNEAGMAIFVQTMINPVSA
CCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCHHEEEEEEECCCCCCEEEHHHHHCCCCC
GTAFSIHPITEEPDYVFIESSFGLGESVTRGMVTPDQYIVSKATRSLVSKRISEKVMKLT
CCEEEEECCCCCCCEEEEECCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
YDFEDRKIKSVELSKEEASAESLSDNDAIRIANMVIAVENIFKRNINIEWAMEDKKVYLL
CCCCCCEEEEEECCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEE
EVRGIRRLYPEF
EECHHHHHCCCC
>Mature Secondary Structure
MNYTYLLEEVSLSMVSIVGRKSAYLGELYKMGFNVPKGFIVSSRGVNEAIKDLDDEIRGI
CCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHH
LSSVNLNDTTDLEKKSEMIKNMIIMSKLPEEMEKEIYERFSQLGSKYVAVRATATSPLSG
HHHCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCCC
ASFAGEYETDLFVTKENLIPSIKRVIASYFNPRAIAYRILTHNEAGMAIFVQTMINPVSA
CCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCHHEEEEEEECCCCCCEEEHHHHHCCCCC
GTAFSIHPITEEPDYVFIESSFGLGESVTRGMVTPDQYIVSKATRSLVSKRISEKVMKLT
CCEEEEECCCCCCCEEEEECCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
YDFEDRKIKSVELSKEEASAESLSDNDAIRIANMVIAVENIFKRNINIEWAMEDKKVYLL
CCCCCCEEEEEECCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEE
EVRGIRRLYPEF
EECHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]