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The map label for this gene is livM [H]

Identifier: 227828703

GI number: 227828703

Start: 2285401

End: 2286447

Strand: Direct

Name: livM [H]

Synonym: M1425_2461

Alternate gene names: 227828703

Gene position: 2285401-2286447 (Clockwise)

Preceding gene: 227828702

Following gene: 227828704

Centisome position: 87.6

GC content: 37.73

Gene sequence:

>1047_bases
ATGGCAGTAGATATTTCCTCTCTTATTTTAGCCATAGTCTTAGACTTCTCAGTATATTTGATGGTTGTAATATCATTAAA
TGTAGAAATAGGATTAACTGGAGTTCCAAATTTTGGTAGAGTCCTAGCGTATGCTGGAGGAGCATTCATAGCTGGAGCAA
TACCGGGAAGGATTTTACAGCTAATATATGGACTTCACAGTGATTATATTCTTAATAATCCCTCTGTAATAGCAGAGATA
AACGATAAATTGCTTCAACAACCGTTAATGGCTCTTGCAATTTTAATTCTTACCCTAATTCTAGCAGGGATAGTTGGGGC
GGCCTTAGGTTATATTTCATCTTATCCTGCTTTGAGATTAAGAGAGGATTACCTGGGAATACTATTATTGGTAGCTGGGA
CTTCACTGGTAGTAATAGGGATTACTTACCCACCAATAGTGGGAGGCACAGTAGGTGTTCAAGTTCCCGCTGTATTTAAC
GCACTTCTAGGTCAAGGATCTAGCTTATATTATAAGATATCAATAGTGTTGTTAGCATTTTCATTAGTGATGCTTCTTCT
ATTCAACTTAACATCGAACTCCCCAATGGGAAGAGCGTTCAGAGCAGTCAGAGAAAATGAGGATCTAGTAGAAGTTCTAG
GTAGAGATATAGGTGTAATCAGAGGTAAGGCAATGGCATTAGGGGGATTTCTAGCGGGCATAGCAGGATCGCTTTACGCT
TTTTATATAGGGTCAGTATTTGCAGGTGCATTCGATAATTTAACGTGGTCATTTAACCCATATTTAATGTTAGTATTGGG
AGGTGCAGGGACTTCAATAGGCGCAATGCTAGGAGCACTAATATATTCCACCTTATATCACGTTATCGATTTGTACAAAC
AAACAATAGGGTATTATCTGCATTTCGATGCAGTATGGCTACAATATATGTTATTTGGACTTATACTAACAATAATCCTT
ATAATACGACCTCAAGGTATTGTCGTAGAAAAAGCTAAGCCATTAGTAAAACCGAAAGCTAAGGATGAGGAAAAACTAAC
TAGGTAA

Upstream 100 bases:

>100_bases
CTTTCGTTATCAACCAATACTCAACGTTAGTACCACTCATAGTAGTTGCAGCAGTTCTCTTAATAGCGCCAAATGGGATT
ATGGGAATAAGGGTGAAATA

Downstream 100 bases:

>100_bases
AGAGAGATTTTTCTGGTTATAAGTTATCTGATACCGTTCATAAAACGCGGGGCGAAGTTTCTATAGATTAAGGGCATCAA
ATGAAAACCAGACCGAATCT

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 348; Mature: 347

Protein sequence:

>348_residues
MAVDISSLILAIVLDFSVYLMVVISLNVEIGLTGVPNFGRVLAYAGGAFIAGAIPGRILQLIYGLHSDYILNNPSVIAEI
NDKLLQQPLMALAILILTLILAGIVGAALGYISSYPALRLREDYLGILLLVAGTSLVVIGITYPPIVGGTVGVQVPAVFN
ALLGQGSSLYYKISIVLLAFSLVMLLLFNLTSNSPMGRAFRAVRENEDLVEVLGRDIGVIRGKAMALGGFLAGIAGSLYA
FYIGSVFAGAFDNLTWSFNPYLMLVLGGAGTSIGAMLGALIYSTLYHVIDLYKQTIGYYLHFDAVWLQYMLFGLILTIIL
IIRPQGIVVEKAKPLVKPKAKDEEKLTR

Sequences:

>Translated_348_residues
MAVDISSLILAIVLDFSVYLMVVISLNVEIGLTGVPNFGRVLAYAGGAFIAGAIPGRILQLIYGLHSDYILNNPSVIAEI
NDKLLQQPLMALAILILTLILAGIVGAALGYISSYPALRLREDYLGILLLVAGTSLVVIGITYPPIVGGTVGVQVPAVFN
ALLGQGSSLYYKISIVLLAFSLVMLLLFNLTSNSPMGRAFRAVRENEDLVEVLGRDIGVIRGKAMALGGFLAGIAGSLYA
FYIGSVFAGAFDNLTWSFNPYLMLVLGGAGTSIGAMLGALIYSTLYHVIDLYKQTIGYYLHFDAVWLQYMLFGLILTIIL
IIRPQGIVVEKAKPLVKPKAKDEEKLTR
>Mature_347_residues
AVDISSLILAIVLDFSVYLMVVISLNVEIGLTGVPNFGRVLAYAGGAFIAGAIPGRILQLIYGLHSDYILNNPSVIAEIN
DKLLQQPLMALAILILTLILAGIVGAALGYISSYPALRLREDYLGILLLVAGTSLVVIGITYPPIVGGTVGVQVPAVFNA
LLGQGSSLYYKISIVLLAFSLVMLLLFNLTSNSPMGRAFRAVRENEDLVEVLGRDIGVIRGKAMALGGFLAGIAGSLYAF
YIGSVFAGAFDNLTWSFNPYLMLVLGGAGTSIGAMLGALIYSTLYHVIDLYKQTIGYYLHFDAVWLQYMLFGLILTIILI
IRPQGIVVEKAKPLVKPKAKDEEKLTR

Specific function: Part of the binding-protein-dependent transport system for branched-chain amino acids. Probably responsible for the translocation of the substrates across the membrane [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=363, Percent_Identity=25.068870523416, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851 [H]

Pfam domain/function: PF02653 BPD_transp_2 [H]

EC number: NA

Molecular weight: Translated: 37349; Mature: 37218

Theoretical pI: Translated: 8.54; Mature: 8.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVDISSLILAIVLDFSVYLMVVISLNVEIGLTGVPNFGRVLAYAGGAFIAGAIPGRILQ
CCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHCCCHHHHHCCHHHHHH
LIYGLHSDYILNNPSVIAEINDKLLQQPLMALAILILTLILAGIVGAALGYISSYPALRL
HHHHCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
REDYLGILLLVAGTSLVVIGITYPPIVGGTVGVQVPAVFNALLGQGSSLYYKISIVLLAF
HHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCEEHHHHHHHHHCCCCCHHHHHHHHHHHH
SLVMLLLFNLTSNSPMGRAFRAVRENEDLVEVLGRDIGVIRGKAMALGGFLAGIAGSLYA
HHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHHHH
FYIGSVFAGAFDNLTWSFNPYLMLVLGGAGTSIGAMLGALIYSTLYHVIDLYKQTIGYYL
HHHHHHHHHHHCCCEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HFDAVWLQYMLFGLILTIILIIRPQGIVVEKAKPLVKPKAKDEEKLTR
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHCCC
>Mature Secondary Structure 
AVDISSLILAIVLDFSVYLMVVISLNVEIGLTGVPNFGRVLAYAGGAFIAGAIPGRILQ
CCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHCCCHHHHHCCHHHHHH
LIYGLHSDYILNNPSVIAEINDKLLQQPLMALAILILTLILAGIVGAALGYISSYPALRL
HHHHCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
REDYLGILLLVAGTSLVVIGITYPPIVGGTVGVQVPAVFNALLGQGSSLYYKISIVLLAF
HHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCEEHHHHHHHHHCCCCCHHHHHHHHHHHH
SLVMLLLFNLTSNSPMGRAFRAVRENEDLVEVLGRDIGVIRGKAMALGGFLAGIAGSLYA
HHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHHHH
FYIGSVFAGAFDNLTWSFNPYLMLVLGGAGTSIGAMLGALIYSTLYHVIDLYKQTIGYYL
HHHHHHHHHHHCCCEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HFDAVWLQYMLFGLILTIILIIRPQGIVVEKAKPLVKPKAKDEEKLTR
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]