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The map label for this gene is livK [C]

Identifier: 227828699

GI number: 227828699

Start: 2281587

End: 2282993

Strand: Direct

Name: livK [C]

Synonym: M1425_2457

Alternate gene names: 227828699

Gene position: 2281587-2282993 (Clockwise)

Preceding gene: 227828698

Following gene: 227828700

Centisome position: 87.46

GC content: 38.17

Gene sequence:

>1407_bases
ATGAAAGGTAAATATAAAAGAGCAATATCAACATCTACTGCAATAATAATTGCTTTGGTTGTAATAATATTAATAGTAGT
AGGTGTAGTAGCATATTTTCAACAGATGGGAAGCCACGCCCCTATGTCGAGTTCTTCTATAACTACTCAATCATCTCTCT
CCTCTACCTCTCAATCTCAAACCAGCGCACAAGAAGTTATTAAGATTGGGGCTTTATTGCCGCTTACGGGACAGTTGTCT
TCTGTTGGAGCTGAGGCACAACAAGTTTTGATGTATGCTCAAGATCAAGCCAATTCTCTACTTAAACAACTAGGTGTAAA
TATGCAAGTTCAAGTAGTAATAGAGGATACTGCAACTAATCCACAGACTGCCCTTTCCGATCTACAGACCTTATACTCTC
AAGGAGTTAGACTAGTAATTGGTCCATTTTCAAGTGGTGAATTAAGGCAAATAATGTCTTATGCTGAGAGTAACGGGATA
ATGCTCTTCTCCCCATCATCCACCTCACCTGCATTAGCAACTCCCGATAGAATTAACGGTACTATATTTAGGGATGTACC
TAATGATACTTATCAAGGGGCTGTAATAGCAAATATAATGTATCAGCAAGGTATTAGGGCAATAGCAGTAATATGGAGAG
GAGATACTTATGGTGATGGATTAGTAAGTGTGGTGAATAAGACATTCACTGCATTAGGCGGAAAAGTAATGCTTGTTGCG
AGATACGATCCTTCTACTACAGACTTTTCAAGCTATGTAAGTACGCTAGCAAATACTATTAGCCAGTTAATTAATCAGTA
TGGTAAAGATCATGTAGCTGTTTACGATGTATCTTTTGAGGAAGGAGCCAGTATACTTCAATTAGCAAGTCAATATCCCC
AATTAACAGAGGTTAAATGGTATGGTTCAGATGGTACTGCCGGTGTTGGATCAATAGTGCAGAATCCAGTAGCTGCCCAG
GTTGCTGTTGAAACATTATTCTTAAATCCCATTGCTGCACCTGCCCACACTGTATTCTATGATCAATTAACTCAGCACTT
CAATAAAACACTTCAAGCTTATTCCTATATAACATATGACATATTCTGGATATACTTCTATAGTGTATTGAAAGTGGGAA
GTGATAATGTAACTGCTATTAAATCAATTTTACCGAAAGTGGCTGCTAAGTTCTATGGTGCGTCTGGATATCACTTGTTT
GACAAATATGGTGATAGAGTATCTGGTGTCTATCAATTGCAGATAGTAAATAAGACAGCTTCTGGTTACGAATGGACTAT
CTACGGTATCTATGATGTTACCACGCATACGTTAACTATAGGATTTCCACCAGCTGCACAACCCCCACAAGGGTATCTAT
ATCCAGATGATCCACAAGCTCAGTCTAAGTTAAGTAGTATAAGTTAA

Upstream 100 bases:

>100_bases
TTTATTTTAAAACATTTATACAGTTTAGATTAATCAGTTTACAGTTAGACAGTTAATATAGTAAAATTTTTATACCGTAC
TAACGCAGTGTTTTAATAGT

Downstream 100 bases:

>100_bases
TATAAGGGGTGTATTTTTTGCCTCTTCTTAAATTGGATTCCGTTTCTAAGTATTTTGGTGGTATTAAGGCATTGGATAAT
GTAACCTTAGAGGTTGATCA

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MKGKYKRAISTSTAIIIALVVIILIVVGVVAYFQQMGSHAPMSSSSITTQSSLSSTSQSQTSAQEVIKIGALLPLTGQLS
SVGAEAQQVLMYAQDQANSLLKQLGVNMQVQVVIEDTATNPQTALSDLQTLYSQGVRLVIGPFSSGELRQIMSYAESNGI
MLFSPSSTSPALATPDRINGTIFRDVPNDTYQGAVIANIMYQQGIRAIAVIWRGDTYGDGLVSVVNKTFTALGGKVMLVA
RYDPSTTDFSSYVSTLANTISQLINQYGKDHVAVYDVSFEEGASILQLASQYPQLTEVKWYGSDGTAGVGSIVQNPVAAQ
VAVETLFLNPIAAPAHTVFYDQLTQHFNKTLQAYSYITYDIFWIYFYSVLKVGSDNVTAIKSILPKVAAKFYGASGYHLF
DKYGDRVSGVYQLQIVNKTASGYEWTIYGIYDVTTHTLTIGFPPAAQPPQGYLYPDDPQAQSKLSSIS

Sequences:

>Translated_468_residues
MKGKYKRAISTSTAIIIALVVIILIVVGVVAYFQQMGSHAPMSSSSITTQSSLSSTSQSQTSAQEVIKIGALLPLTGQLS
SVGAEAQQVLMYAQDQANSLLKQLGVNMQVQVVIEDTATNPQTALSDLQTLYSQGVRLVIGPFSSGELRQIMSYAESNGI
MLFSPSSTSPALATPDRINGTIFRDVPNDTYQGAVIANIMYQQGIRAIAVIWRGDTYGDGLVSVVNKTFTALGGKVMLVA
RYDPSTTDFSSYVSTLANTISQLINQYGKDHVAVYDVSFEEGASILQLASQYPQLTEVKWYGSDGTAGVGSIVQNPVAAQ
VAVETLFLNPIAAPAHTVFYDQLTQHFNKTLQAYSYITYDIFWIYFYSVLKVGSDNVTAIKSILPKVAAKFYGASGYHLF
DKYGDRVSGVYQLQIVNKTASGYEWTIYGIYDVTTHTLTIGFPPAAQPPQGYLYPDDPQAQSKLSSIS
>Mature_468_residues
MKGKYKRAISTSTAIIIALVVIILIVVGVVAYFQQMGSHAPMSSSSITTQSSLSSTSQSQTSAQEVIKIGALLPLTGQLS
SVGAEAQQVLMYAQDQANSLLKQLGVNMQVQVVIEDTATNPQTALSDLQTLYSQGVRLVIGPFSSGELRQIMSYAESNGI
MLFSPSSTSPALATPDRINGTIFRDVPNDTYQGAVIANIMYQQGIRAIAVIWRGDTYGDGLVSVVNKTFTALGGKVMLVA
RYDPSTTDFSSYVSTLANTISQLINQYGKDHVAVYDVSFEEGASILQLASQYPQLTEVKWYGSDGTAGVGSIVQNPVAAQ
VAVETLFLNPIAAPAHTVFYDQLTQHFNKTLQAYSYITYDIFWIYFYSVLKVGSDNVTAIKSILPKVAAKFYGASGYHLF
DKYGDRVSGVYQLQIVNKTASGYEWTIYGIYDVTTHTLTIGFPPAAQPPQGYLYPDDPQAQSKLSSIS

Specific function: This Protein Is A Component Of The Leucine-Specific Transport System, Which Is One Of The Two Periplasmic Binding Protein-Dependent Transport Systems Of The High-Affinity Transport Of The Branched-Chain Amino Acids In E.Coli. [C]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 50650; Mature: 50650

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGKYKRAISTSTAIIIALVVIILIVVGVVAYFQQMGSHAPMSSSSITTQSSLSSTSQSQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCHHHH
TSAQEVIKIGALLPLTGQLSSVGAEAQQVLMYAQDQANSLLKQLGVNMQVQVVIEDTATN
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCC
PQTALSDLQTLYSQGVRLVIGPFSSGELRQIMSYAESNGIMLFSPSSTSPALATPDRING
HHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCC
TIFRDVPNDTYQGAVIANIMYQQGIRAIAVIWRGDTYGDGLVSVVNKTFTALGGKVMLVA
EEEECCCCCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEE
RYDPSTTDFSSYVSTLANTISQLINQYGKDHVAVYDVSFEEGASILQLASQYPQLTEVKW
ECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHCHHHHHHHHHCCCCEEEEE
YGSDGTAGVGSIVQNPVAAQVAVETLFLNPIAAPAHTVFYDQLTQHFNKTLQAYSYITYD
ECCCCCCCHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IFWIYFYSVLKVGSDNVTAIKSILPKVAAKFYGASGYHLFDKYGDRVSGVYQLQIVNKTA
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCEEEEEEEECCC
SGYEWTIYGIYDVTTHTLTIGFPPAAQPPQGYLYPDDPQAQSKLSSIS
CCCEEEEEEEEEEEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHCCC
>Mature Secondary Structure
MKGKYKRAISTSTAIIIALVVIILIVVGVVAYFQQMGSHAPMSSSSITTQSSLSSTSQSQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCHHHH
TSAQEVIKIGALLPLTGQLSSVGAEAQQVLMYAQDQANSLLKQLGVNMQVQVVIEDTATN
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCC
PQTALSDLQTLYSQGVRLVIGPFSSGELRQIMSYAESNGIMLFSPSSTSPALATPDRING
HHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCC
TIFRDVPNDTYQGAVIANIMYQQGIRAIAVIWRGDTYGDGLVSVVNKTFTALGGKVMLVA
EEEECCCCCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEE
RYDPSTTDFSSYVSTLANTISQLINQYGKDHVAVYDVSFEEGASILQLASQYPQLTEVKW
ECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHCHHHHHHHHHCCCCEEEEE
YGSDGTAGVGSIVQNPVAAQVAVETLFLNPIAAPAHTVFYDQLTQHFNKTLQAYSYITYD
ECCCCCCCHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IFWIYFYSVLKVGSDNVTAIKSILPKVAAKFYGASGYHLFDKYGDRVSGVYQLQIVNKTA
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCEEEEEEEECCC
SGYEWTIYGIYDVTTHTLTIGFPPAAQPPQGYLYPDDPQAQSKLSSIS
CCCEEEEEEEEEEEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]