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The map label for this gene is yeiK [C]

Identifier: 227828547

GI number: 227828547

Start: 2103532

End: 2104473

Strand: Direct

Name: yeiK [C]

Synonym: M1425_2298

Alternate gene names: 227828547

Gene position: 2103532-2104473 (Clockwise)

Preceding gene: 227828540

Following gene: 227828549

Centisome position: 80.63

GC content: 33.55

Gene sequence:

>942_bases
ATGAGATACTTCATAATAGACTGTGACACTGCAGAAGATGATATAATGAGTTTGATAATGCTGATTAAAAACAATATACA
AGTTGTCGGGATTACTATTGTTGAAGGTAATGTAAACTTTAATCAGCAAGTAGACACTATGTTATGGGCTTTAGAGTTTC
TAAATATCGATATTCCAGTATATCCAAATTCAAAAAGACCACTAGTTAAGTCTTTTAAAACCGTAGAAGATGTTCATGGA
AAGGGAGGAGTAGGAAATGAGATTGTAAGACCAATAAGACTTAAGGCACGAAGTAAGCATGCTGTTGATGCTATAACAGA
GTTATGTGAAACGTATTTCAAGATATTAGAGTTTTTAGCTATCTCTCCGTTAACTAATTTAGCCTTAGCATATCTAAAGT
ACCCTAGATTAACTGAATGTATACATCACCTATACATTATGGGTGGTACAATCTATGGAAGAGGCAACATTACACCCTTA
GCAGAATATGATTTTTCGGTAGACCCAGATGCCGTAAAGATAGCATTACACGCTGGTTTCACTAACGTAACATTAGTTCC
TTGGGAGGTTACAGTGGCTAATGCTATTGATCCTAATATCTGGAATTTCATAAAATCCCTTAATACCAAACTTTCTAATT
TTTACGTTAAGATTTATTCACATTATAGAACATTTTCAATGCAGATACAAAAAATGAGGGGAAATCCACATTCAGACGTT
ATTACAGCAGTCATAGCCATTGAACCAAATATTATTAAAAAATCTCATAAAGAGTACGTAGATGTTGAAACTAATGAGGG
GTTAACTAGAGGTGTCACTATAATAGATTACGTGGACCTTGGACATATTATTGGTAACAATAAACCTAACGCCGAAGTCG
TATATGACATCGATTATGAGAAATTTGTCCAAATCTTACTAAAAACTTTAAGTTCAGATTGA

Upstream 100 bases:

>100_bases
CTCCTTCAAAAATATTCATATTTTGCGCTGTAGCTAGAGCATCGGCTCACTCATAGGGGCATTGTTATATTACTTATATT
AAATGGAAAAAGTGAAATAG

Downstream 100 bases:

>100_bases
GTTAATCTATCAAGTCAGAGATTTTTTTAAGTATCTTCCTTAAAGAGAAAGAGATATTCATCTTACTAACTCCTAATTGT
TTAGCTAGTTCTTGAGAGTT

Product: inosine/uridine-preferring nucleoside hydrolase

Products: D-ribose; purine

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MRYFIIDCDTAEDDIMSLIMLIKNNIQVVGITIVEGNVNFNQQVDTMLWALEFLNIDIPVYPNSKRPLVKSFKTVEDVHG
KGGVGNEIVRPIRLKARSKHAVDAITELCETYFKILEFLAISPLTNLALAYLKYPRLTECIHHLYIMGGTIYGRGNITPL
AEYDFSVDPDAVKIALHAGFTNVTLVPWEVTVANAIDPNIWNFIKSLNTKLSNFYVKIYSHYRTFSMQIQKMRGNPHSDV
ITAVIAIEPNIIKKSHKEYVDVETNEGLTRGVTIIDYVDLGHIIGNNKPNAEVVYDIDYEKFVQILLKTLSSD

Sequences:

>Translated_313_residues
MRYFIIDCDTAEDDIMSLIMLIKNNIQVVGITIVEGNVNFNQQVDTMLWALEFLNIDIPVYPNSKRPLVKSFKTVEDVHG
KGGVGNEIVRPIRLKARSKHAVDAITELCETYFKILEFLAISPLTNLALAYLKYPRLTECIHHLYIMGGTIYGRGNITPL
AEYDFSVDPDAVKIALHAGFTNVTLVPWEVTVANAIDPNIWNFIKSLNTKLSNFYVKIYSHYRTFSMQIQKMRGNPHSDV
ITAVIAIEPNIIKKSHKEYVDVETNEGLTRGVTIIDYVDLGHIIGNNKPNAEVVYDIDYEKFVQILLKTLSSD
>Mature_313_residues
MRYFIIDCDTAEDDIMSLIMLIKNNIQVVGITIVEGNVNFNQQVDTMLWALEFLNIDIPVYPNSKRPLVKSFKTVEDVHG
KGGVGNEIVRPIRLKARSKHAVDAITELCETYFKILEFLAISPLTNLALAYLKYPRLTECIHHLYIMGGTIYGRGNITPL
AEYDFSVDPDAVKIALHAGFTNVTLVPWEVTVANAIDPNIWNFIKSLNTKLSNFYVKIYSHYRTFSMQIQKMRGNPHSDV
ITAVIAIEPNIIKKSHKEYVDVETNEGLTRGVTIIDYVDLGHIIGNNKPNAEVVYDIDYEKFVQILLKTLSSD

Specific function: Unknown

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family [H]

Homologues:

Organism=Escherichia coli, GI1788486, Length=303, Percent_Identity=27.3927392739274, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1786871, Length=309, Percent_Identity=27.1844660194175, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1786213, Length=283, Percent_Identity=30.3886925795053, Blast_Score=108, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI32565474, Length=293, Percent_Identity=31.3993174061433, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17565698, Length=300, Percent_Identity=28, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6320608, Length=175, Percent_Identity=28.5714285714286, Blast_Score=73, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24641837, Length=304, Percent_Identity=27.6315789473684, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24583915, Length=330, Percent_Identity=26.0606060606061, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24641839, Length=299, Percent_Identity=27.7591973244147, Blast_Score=101, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: 3.2.2.1

Molecular weight: Translated: 35455; Mature: 35455

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYFIIDCDTAEDDIMSLIMLIKNNIQVVGITIVEGNVNFNQQVDTMLWALEFLNIDIPV
CEEEEEECCCCHHHHHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHEEEEEE
YPNSKRPLVKSFKTVEDVHGKGGVGNEIVRPIRLKARSKHAVDAITELCETYFKILEFLA
CCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
ISPLTNLALAYLKYPRLTECIHHLYIMGGTIYGRGNITPLAEYDFSVDPDAVKIALHAGF
HCCHHHHHHHHHCCCCHHHHHHHHHHHCCEEEECCCCCEEECCCCCCCCCCEEEEEECCC
TNVTLVPWEVTVANAIDPNIWNFIKSLNTKLSNFYVKIYSHYRTFSMQIQKMRGNPHSDV
CEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHCCCCCHHH
ITAVIAIEPNIIKKSHKEYVDVETNEGLTRGVTIIDYVDLGHIIGNNKPNAEVVYDIDYE
EEEEEEECCHHHHHCCHHEEEEECCCCCCCCEEEEEEEHHHHHHCCCCCCCEEEEECCHH
KFVQILLKTLSSD
HHHHHHHHHHCCC
>Mature Secondary Structure
MRYFIIDCDTAEDDIMSLIMLIKNNIQVVGITIVEGNVNFNQQVDTMLWALEFLNIDIPV
CEEEEEECCCCHHHHHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHEEEEEE
YPNSKRPLVKSFKTVEDVHGKGGVGNEIVRPIRLKARSKHAVDAITELCETYFKILEFLA
CCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
ISPLTNLALAYLKYPRLTECIHHLYIMGGTIYGRGNITPLAEYDFSVDPDAVKIALHAGF
HCCHHHHHHHHHCCCCHHHHHHHHHHHCCEEEECCCCCEEECCCCCCCCCCEEEEEECCC
TNVTLVPWEVTVANAIDPNIWNFIKSLNTKLSNFYVKIYSHYRTFSMQIQKMRGNPHSDV
CEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHCCCCCHHH
ITAVIAIEPNIIKKSHKEYVDVETNEGLTRGVTIIDYVDLGHIIGNNKPNAEVVYDIDYE
EEEEEEECCHHHHHCCHHEEEEECCCCCCCCEEEEEEEHHHHHHCCCCCCCEEEEECCHH
KFVQILLKTLSSD
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-D-ribosylpurine; H2O

Specific reaction: an N-D-ribosylpurine + H2O = D-ribose + a purine

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA