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The map label for this gene is ilvD

Identifier: 227828508

GI number: 227828508

Start: 2065666

End: 2067342

Strand: Direct

Name: ilvD

Synonym: M1425_2257

Alternate gene names: 227828508

Gene position: 2065666-2067342 (Clockwise)

Preceding gene: 227828502

Following gene: 227828509

Centisome position: 79.18

GC content: 43.23

Gene sequence:

>1677_bases
ATGCCAGCAAAATTAAATAGTCCCTCGAGATATCATGGCATATATAATGCTCCACACAGAGCGTTTCTAAGATCTGTAGG
TTTAACGGATGAAGAGATAGGTAAACCATTGGTTGCCATAGCCACAGCATGGAGTGAGGCAGGCCCTTGTAACTTCCACA
CATTAGCTTTAGCAAGAGTAGCTAAGGAGGGGACGAAGGAAGCCGGTTTATCCCCATTAGCATTCCCAACCATGGTAGTT
AACGATAACATTGGAATGGGATCTGAAGGAATGAGGTATAGTTTAGTTAGCAGGGATTTAATAGCAGATATGGTAGAGGC
GCAATTTAATGCTCACGCGTTTGATGGACTAGTGGGCATAGGAGGGTGTGATAAGACAACGCCCGGTATACTAATGGCAA
TGGCTAGGTTAAACGTTCCCTCAATATACATTTATGGCGGATCAGCAGAGCCAGGGTACTTTATGGGTAAAAGACTAACA
ATAGAGGATGTACATGAGGCAATAGGCGCATATTTAGCGAAGAGAATAACAGAAAATGAGTTATACGAAATAGAGAAAAG
GGCACATCCGACATTGGGAACTTGCTCTGGATTATTTACAGCCAATACTATGGGCTCAATGTCGGAGGCATTAGGAATGG
CATTGCCAGGTAGTGCATCACCAACCGCTACCTCATCTAGGAGAGTAATGTATGTGAGGGAAACTGGAAAAGCGTTAGGT
AGTTTAATTGAAAACGGAATAAAGTCAAGGGACATATTAACTTTTGAGGCCTTTGAGAACGCAATAACAACGCTAATGGC
TATGGGTGGTTCAACAAACGCGGTATTACACCTATTGGCAATAGCCTATGAAGCAGGAGTGAAATTAACCTTAGATGATT
TCAATAGGATATCTAAAAGAACACCATATATTGCAAGTATGAAACCTGGTGGAGATTACGTGATGGCTGATTTGGATGAA
GTTGGAGGTGTTCCAGTAGTCTTAAAGAAACTACTAGACGCTGGTCTACTTCATGGTGACGTTTTAACAGTTACTGGAAA
GACTATGAAGCAAAACCTTGAGCAATACAAGTATCCTAATGTACCTCACAGTCATATAGTTAGGGATGTTAAGAATCCGA
TTAAGCCTAGAGGAGGAATAGTTATATTGAAGGGATCGTTAGCCCCAGAAGGTGCCGTAATTAAGGTAGCCGCAACTAAC
GTTGTTAAATTTGAAGGAAAGGCTAAGGTGTATAATTCCGAGGACGACGCTTTTAAGGGAGTTCAAAGTGGCGAAGTCAG
GGAGGGTGAAGTTGTAATTATAAGATATGAAGGACCTAAGGGAGCTCCAGGTATGCCAGAAATGCTGAGAGTTACAGCGG
CAATCATGGGTGCGGGTTTGAATAACGTTGCCTTAGTTACTGATGGTAGATTTTCCGGAGCCACTAGGGGACCTATGGTA
GGCCATGTAGCTCCAGAGGCAATGGTAGGTGGTCCTATCGCAATAGTTGAAGATGGAGATACTATAGTGATTGATGTGGA
GAGCGAAAGACTTGACTTGAAGCTATCCGAGGAGGAGATAAAGAATAGACTGAAAAGATGGTCTCCTCCATCTCCAAGAT
ACAAGTCTGGTCTATTGGCTAAATACGCCTCCTTGGTCTCCCAGGCGTCAATGGGGGCTGTGACTAGACCAGCTTAA

Upstream 100 bases:

>100_bases
TGAAATGGTTCTACTGGGAGGGAAGACATACTAAAATCATCCTTGTTAAACCTAACTTGTAAACATGATCACTTATTAGT
TTCAGTTTAGATTTCTCTAT

Downstream 100 bases:

>100_bases
TTTTTTATTTAATTCTCCTAATTCTCCTTTATGTATGAAGACATTTGGGCAATTGAACACGCAATAAGCTTACTGGATTG
GGATATCCAAACTTACATGC

Product: dihydroxy-acid dehydratase

Products: NA

Alternate protein names: DAD

Number of amino acids: Translated: 558; Mature: 557

Protein sequence:

>558_residues
MPAKLNSPSRYHGIYNAPHRAFLRSVGLTDEEIGKPLVAIATAWSEAGPCNFHTLALARVAKEGTKEAGLSPLAFPTMVV
NDNIGMGSEGMRYSLVSRDLIADMVEAQFNAHAFDGLVGIGGCDKTTPGILMAMARLNVPSIYIYGGSAEPGYFMGKRLT
IEDVHEAIGAYLAKRITENELYEIEKRAHPTLGTCSGLFTANTMGSMSEALGMALPGSASPTATSSRRVMYVRETGKALG
SLIENGIKSRDILTFEAFENAITTLMAMGGSTNAVLHLLAIAYEAGVKLTLDDFNRISKRTPYIASMKPGGDYVMADLDE
VGGVPVVLKKLLDAGLLHGDVLTVTGKTMKQNLEQYKYPNVPHSHIVRDVKNPIKPRGGIVILKGSLAPEGAVIKVAATN
VVKFEGKAKVYNSEDDAFKGVQSGEVREGEVVIIRYEGPKGAPGMPEMLRVTAAIMGAGLNNVALVTDGRFSGATRGPMV
GHVAPEAMVGGPIAIVEDGDTIVIDVESERLDLKLSEEEIKNRLKRWSPPSPRYKSGLLAKYASLVSQASMGAVTRPA

Sequences:

>Translated_558_residues
MPAKLNSPSRYHGIYNAPHRAFLRSVGLTDEEIGKPLVAIATAWSEAGPCNFHTLALARVAKEGTKEAGLSPLAFPTMVV
NDNIGMGSEGMRYSLVSRDLIADMVEAQFNAHAFDGLVGIGGCDKTTPGILMAMARLNVPSIYIYGGSAEPGYFMGKRLT
IEDVHEAIGAYLAKRITENELYEIEKRAHPTLGTCSGLFTANTMGSMSEALGMALPGSASPTATSSRRVMYVRETGKALG
SLIENGIKSRDILTFEAFENAITTLMAMGGSTNAVLHLLAIAYEAGVKLTLDDFNRISKRTPYIASMKPGGDYVMADLDE
VGGVPVVLKKLLDAGLLHGDVLTVTGKTMKQNLEQYKYPNVPHSHIVRDVKNPIKPRGGIVILKGSLAPEGAVIKVAATN
VVKFEGKAKVYNSEDDAFKGVQSGEVREGEVVIIRYEGPKGAPGMPEMLRVTAAIMGAGLNNVALVTDGRFSGATRGPMV
GHVAPEAMVGGPIAIVEDGDTIVIDVESERLDLKLSEEEIKNRLKRWSPPSPRYKSGLLAKYASLVSQASMGAVTRPA
>Mature_557_residues
PAKLNSPSRYHGIYNAPHRAFLRSVGLTDEEIGKPLVAIATAWSEAGPCNFHTLALARVAKEGTKEAGLSPLAFPTMVVN
DNIGMGSEGMRYSLVSRDLIADMVEAQFNAHAFDGLVGIGGCDKTTPGILMAMARLNVPSIYIYGGSAEPGYFMGKRLTI
EDVHEAIGAYLAKRITENELYEIEKRAHPTLGTCSGLFTANTMGSMSEALGMALPGSASPTATSSRRVMYVRETGKALGS
LIENGIKSRDILTFEAFENAITTLMAMGGSTNAVLHLLAIAYEAGVKLTLDDFNRISKRTPYIASMKPGGDYVMADLDEV
GGVPVVLKKLLDAGLLHGDVLTVTGKTMKQNLEQYKYPNVPHSHIVRDVKNPIKPRGGIVILKGSLAPEGAVIKVAATNV
VKFEGKAKVYNSEDDAFKGVQSGEVREGEVVIIRYEGPKGAPGMPEMLRVTAAIMGAGLNNVALVTDGRFSGATRGPMVG
HVAPEAMVGGPIAIVEDGDTIVIDVESERLDLKLSEEEIKNRLKRWSPPSPRYKSGLLAKYASLVSQASMGAVTRPA

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family

Homologues:

Organism=Escherichia coli, GI48994964, Length=609, Percent_Identity=38.2594417077176, Blast_Score=389, Evalue=1e-109,
Organism=Escherichia coli, GI2367371, Length=457, Percent_Identity=34.7921225382932, Blast_Score=210, Evalue=2e-55,
Organism=Escherichia coli, GI1786464, Length=496, Percent_Identity=33.8709677419355, Blast_Score=206, Evalue=3e-54,
Organism=Escherichia coli, GI1788157, Length=473, Percent_Identity=32.5581395348837, Blast_Score=205, Evalue=5e-54,
Organism=Saccharomyces cerevisiae, GI6322476, Length=545, Percent_Identity=45.8715596330275, Blast_Score=486, Evalue=1e-138,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ILVD_SULIA (C3N1G8)

Other databases:

- EMBL:   CP001401
- RefSeq:   YP_002844240.1
- GeneID:   7814327
- GenomeReviews:   CP001401_GR
- KEGG:   sim:M1627_2336
- OMA:   NMPGAMI
- ProtClustDB:   PRK00911
- HAMAP:   MF_00012
- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558
- PANTHER:   PTHR21000
- TIGRFAMs:   TIGR00110

Pfam domain/function: PF00920 ILVD_EDD; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.9

Molecular weight: Translated: 59505; Mature: 59374

Theoretical pI: Translated: 7.72; Mature: 7.72

Prosite motif: PS00886 ILVD_EDD_1; PS00887 ILVD_EDD_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAKLNSPSRYHGIYNAPHRAFLRSVGLTDEEIGKPLVAIATAWSEAGPCNFHTLALARV
CCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHCCCEEEEEECCCCCCCCCHHHHHHHHH
AKEGTKEAGLSPLAFPTMVVNDNIGMGSEGMRYSLVSRDLIADMVEAQFNAHAFDGLVGI
HHHCCHHCCCCCCCCCEEEEECCCCCCCCCCEEHHHHHHHHHHHHHHHCCCHHHCCEEEC
GGCDKTTPGILMAMARLNVPSIYIYGGSAEPGYFMGKRLTIEDVHEAIGAYLAKRITENE
CCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCHHH
LYEIEKRAHPTLGTCSGLFTANTMGSMSEALGMALPGSASPTATSSRRVMYVRETGKALG
HHHHHHHCCCCCCCCCCCEECCCHHHHHHHHCCCCCCCCCCCCCCCCEEEEEHHHHHHHH
SLIENGIKSRDILTFEAFENAITTLMAMGGSTNAVLHLLAIAYEAGVKLTLDDFNRISKR
HHHHCCCCCCCEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEHHHHHHHHHC
TPYIASMKPGGDYVMADLDEVGGVPVVLKKLLDAGLLHGDVLTVTGKTMKQNLEQYKYPN
CCEEEEECCCCCEEEEEHHHHCCHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHCCCC
VPHSHIVRDVKNPIKPRGGIVILKGSLAPEGAVIKVAATNVVKFEGKAKVYNSEDDAFKG
CCHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEEEECEEEEECCCEEEECCCCHHHCC
VQSGEVREGEVVIIRYEGPKGAPGMPEMLRVTAAIMGAGLNNVALVTDGRFSGATRGPMV
CCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEE
GHVAPEAMVGGPIAIVEDGDTIVIDVESERLDLKLSEEEIKNRLKRWSPPSPRYKSGLLA
ECCCCHHHCCCCEEEEECCCEEEEEECCCCEEEEECHHHHHHHHHCCCCCCCCHHHHHHH
KYASLVSQASMGAVTRPA
HHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
PAKLNSPSRYHGIYNAPHRAFLRSVGLTDEEIGKPLVAIATAWSEAGPCNFHTLALARV
CCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHCCCEEEEEECCCCCCCCCHHHHHHHHH
AKEGTKEAGLSPLAFPTMVVNDNIGMGSEGMRYSLVSRDLIADMVEAQFNAHAFDGLVGI
HHHCCHHCCCCCCCCCEEEEECCCCCCCCCCEEHHHHHHHHHHHHHHHCCCHHHCCEEEC
GGCDKTTPGILMAMARLNVPSIYIYGGSAEPGYFMGKRLTIEDVHEAIGAYLAKRITENE
CCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCHHH
LYEIEKRAHPTLGTCSGLFTANTMGSMSEALGMALPGSASPTATSSRRVMYVRETGKALG
HHHHHHHCCCCCCCCCCCEECCCHHHHHHHHCCCCCCCCCCCCCCCCEEEEEHHHHHHHH
SLIENGIKSRDILTFEAFENAITTLMAMGGSTNAVLHLLAIAYEAGVKLTLDDFNRISKR
HHHHCCCCCCCEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEHHHHHHHHHC
TPYIASMKPGGDYVMADLDEVGGVPVVLKKLLDAGLLHGDVLTVTGKTMKQNLEQYKYPN
CCEEEEECCCCCEEEEEHHHHCCHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHCCCC
VPHSHIVRDVKNPIKPRGGIVILKGSLAPEGAVIKVAATNVVKFEGKAKVYNSEDDAFKG
CCHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEEEECEEEEECCCEEEECCCCHHHCC
VQSGEVREGEVVIIRYEGPKGAPGMPEMLRVTAAIMGAGLNNVALVTDGRFSGATRGPMV
CCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEE
GHVAPEAMVGGPIAIVEDGDTIVIDVESERLDLKLSEEEIKNRLKRWSPPSPRYKSGLLA
ECCCCHHHCCCCEEEEECCCEEEEEECCCCEEEEECHHHHHHHHHCCCCCCCCHHHHHHH
KYASLVSQASMGAVTRPA
HHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA