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The map label for this gene is rhmD [H]

Identifier: 227828492

GI number: 227828492

Start: 2050239

End: 2051360

Strand: Direct

Name: rhmD [H]

Synonym: M1425_2241

Alternate gene names: 227828492

Gene position: 2050239-2051360 (Clockwise)

Preceding gene: 227828490

Following gene: 227828493

Centisome position: 78.59

GC content: 40.02

Gene sequence:

>1122_bases
ATGATAAAGGATATTAGAACGTATAAGTTATGTTACGAAGGGATAAACGATGAAAGGGATGCGTTAGCAATTAAGGGGTT
AGCAGAGCACCCAATGGAGATTGTAGTAACTGAAATCGAGACTTCTGACGGTTATGTAGGTTATGGTGAGTCGTTGGCTT
ACGGGTGTTCAGATGCAGTTCAAGTTACAATAGAGAAAATATTGAAACCGTTGCTCCTTAAGGAAGACGAGGAACTCATT
GAGTACTTATGGGATAAAATGTATAAAGCTACCTTAAGGTTTGGTAGAAGAGGAATAGCCATAGCTGGTATTAGTGGTGT
AGATACTGGCCTATGGGATATAATGGGGAAAAAGGCGAAGAAACCAATTTATAAATTACTTGGAGGTAGCAAAAGGAAAG
TAAGAGCGTATATAACTGGAGGTTACTATTCCGAAAAGAAGGATCTTGAAAAACTTAGAGATGAGGAAGCGTACTACGTT
AAAATGGGATTTAAGGGAATTAAGGTAAAGATTGGTGCTAAAAGTATGGAGGAAGATATCGAGAGGCTCAAGGCCATAAG
GGAAGTGGTTGGAGAGGATGTTAAAATAGCTGTGGATGCTAATAACGTTTACACGTTTGAAGAAGCATTGGAAATGGGAA
GAAGGTTAGAGAAATTGGGGATATGGTTCTTTGAAGAGCCAATTCAAACTGATTATCTTGATCTAAGTGCCAGACTAGCA
GAGGAGTTGGAAGTTCCAATAGCCGGCTACGAGACTGCTTATACTAGGTGGGAATTTTACGAGATAATGAGGAAGAGGGC
AGTCGATATTGTGCAAACAGATGTAATGTGGACAGGAGGAATATCTGAGATGATGAAAATAGGGAATATGGCCAAGGTAA
TGGGATATCCGTTAATCCCACATTATTCTGCAGGGGGTATATCGCTTATTGGCAATTTGCATGTAGCTGCGGCGTTAAAT
TCGCCTTGGATAGAGATGCATCTAAGGAAAAATGACTTAAGAGACAAAATATTCAAGGAAAGCATTGAAATTGACAATGG
CCACTTAGTCGTTCCGGATAGACCGGGATTAGGTTACACAATTAGAGATGGAGTGTTTGAAGAGTACAAGTGCAAGTCGT
GA

Upstream 100 bases:

>100_bases
TCTTCATGAAAAATAAAATCTGCAAAAGTACTTATTAATTTCTCTCAAACTTTACCTTGTTAGGATTGATGTTTATAAGT
ATAATTGGCTATTTACTATC

Downstream 100 bases:

>100_bases
ATTCTTTTTTATCCCAATAACTAAAACTTCCTATTATGGAGAGAATAGGAGATTTACTTTCTAACCTTCCTACCGACTAC
GCTAAGGCACTAATTCAAAT

Product: mandelate racemase/muconate lactonizing protein

Products: NA

Alternate protein names: RhamD [H]

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MIKDIRTYKLCYEGINDERDALAIKGLAEHPMEIVVTEIETSDGYVGYGESLAYGCSDAVQVTIEKILKPLLLKEDEELI
EYLWDKMYKATLRFGRRGIAIAGISGVDTGLWDIMGKKAKKPIYKLLGGSKRKVRAYITGGYYSEKKDLEKLRDEEAYYV
KMGFKGIKVKIGAKSMEEDIERLKAIREVVGEDVKIAVDANNVYTFEEALEMGRRLEKLGIWFFEEPIQTDYLDLSARLA
EELEVPIAGYETAYTRWEFYEIMRKRAVDIVQTDVMWTGGISEMMKIGNMAKVMGYPLIPHYSAGGISLIGNLHVAAALN
SPWIEMHLRKNDLRDKIFKESIEIDNGHLVVPDRPGLGYTIRDGVFEEYKCKS

Sequences:

>Translated_373_residues
MIKDIRTYKLCYEGINDERDALAIKGLAEHPMEIVVTEIETSDGYVGYGESLAYGCSDAVQVTIEKILKPLLLKEDEELI
EYLWDKMYKATLRFGRRGIAIAGISGVDTGLWDIMGKKAKKPIYKLLGGSKRKVRAYITGGYYSEKKDLEKLRDEEAYYV
KMGFKGIKVKIGAKSMEEDIERLKAIREVVGEDVKIAVDANNVYTFEEALEMGRRLEKLGIWFFEEPIQTDYLDLSARLA
EELEVPIAGYETAYTRWEFYEIMRKRAVDIVQTDVMWTGGISEMMKIGNMAKVMGYPLIPHYSAGGISLIGNLHVAAALN
SPWIEMHLRKNDLRDKIFKESIEIDNGHLVVPDRPGLGYTIRDGVFEEYKCKS
>Mature_373_residues
MIKDIRTYKLCYEGINDERDALAIKGLAEHPMEIVVTEIETSDGYVGYGESLAYGCSDAVQVTIEKILKPLLLKEDEELI
EYLWDKMYKATLRFGRRGIAIAGISGVDTGLWDIMGKKAKKPIYKLLGGSKRKVRAYITGGYYSEKKDLEKLRDEEAYYV
KMGFKGIKVKIGAKSMEEDIERLKAIREVVGEDVKIAVDANNVYTFEEALEMGRRLEKLGIWFFEEPIQTDYLDLSARLA
EELEVPIAGYETAYTRWEFYEIMRKRAVDIVQTDVMWTGGISEMMKIGNMAKVMGYPLIPHYSAGGISLIGNLHVAAALN
SPWIEMHLRKNDLRDKIFKESIEIDNGHLVVPDRPGLGYTIRDGVFEEYKCKS

Specific function: Catalyzes the dehydration of L-rhamnonate to 2-keto-3- deoxy-L-rhamnonate (KDR) [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. RhamD subfamily [H]

Homologues:

Organism=Homo sapiens, GI42544119, Length=399, Percent_Identity=23.0576441102757, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI186972148, Length=344, Percent_Identity=23.546511627907, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI42544117, Length=398, Percent_Identity=22.1105527638191, Blast_Score=79, Evalue=6e-15,
Organism=Escherichia coli, GI48994953, Length=353, Percent_Identity=28.328611898017, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI1787864, Length=370, Percent_Identity=25.1351351351351, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI226510960, Length=345, Percent_Identity=26.9565217391304, Blast_Score=100, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =4.2.1.90 [H]

Molecular weight: Translated: 42452; Mature: 42452

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKDIRTYKLCYEGINDERDALAIKGLAEHPMEIVVTEIETSDGYVGYGESLAYGCSDAV
CCCCHHHHHHHHCCCCCCCHHHEECCHHHCCEEEEEEEEECCCCEEECCCCHHCCCCHHH
QVTIEKILKPLLLKEDEELIEYLWDKMYKATLRFGRRGIAIAGISGVDTGLWDIMGKKAK
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHC
KPIYKLLGGSKRKVRAYITGGYYSEKKDLEKLRDEEAYYVKMGFKGIKVKIGAKSMEEDI
CHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHCCCCEEEEEECCCEEEEEECCHHHHHHH
ERLKAIREVVGEDVKIAVDANNVYTFEEALEMGRRLEKLGIWFFEEPIQTDYLDLSARLA
HHHHHHHHHCCCCEEEEEECCCEEEHHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHH
EELEVPIAGYETAYTRWEFYEIMRKRAVDIVQTDVMWTGGISEMMKIGNMAKVMGYPLIP
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEECCCHHHHHHHCCHHHHHCCCCCC
HYSAGGISLIGNLHVAAALNSPWIEMHLRKNDLRDKIFKESIEIDNGHLVVPDRPGLGYT
CCCCCCEEEEECEEEEEECCCCEEEEEECHHHHHHHHHHHHCCCCCCEEEECCCCCCCEE
IRDGVFEEYKCKS
CCCCCCCCCCCCC
>Mature Secondary Structure
MIKDIRTYKLCYEGINDERDALAIKGLAEHPMEIVVTEIETSDGYVGYGESLAYGCSDAV
CCCCHHHHHHHHCCCCCCCHHHEECCHHHCCEEEEEEEEECCCCEEECCCCHHCCCCHHH
QVTIEKILKPLLLKEDEELIEYLWDKMYKATLRFGRRGIAIAGISGVDTGLWDIMGKKAK
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHC
KPIYKLLGGSKRKVRAYITGGYYSEKKDLEKLRDEEAYYVKMGFKGIKVKIGAKSMEEDI
CHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHCCCCEEEEEECCCEEEEEECCHHHHHHH
ERLKAIREVVGEDVKIAVDANNVYTFEEALEMGRRLEKLGIWFFEEPIQTDYLDLSARLA
HHHHHHHHHCCCCEEEEEECCCEEEHHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHH
EELEVPIAGYETAYTRWEFYEIMRKRAVDIVQTDVMWTGGISEMMKIGNMAKVMGYPLIP
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEECCCHHHHHHHCCHHHHHCCCCCC
HYSAGGISLIGNLHVAAALNSPWIEMHLRKNDLRDKIFKESIEIDNGHLVVPDRPGLGYT
CCCCCCEEEEECEEEEEECCCCEEEEEECHHHHHHHHHHHHCCCCCCEEEECCCCCCCEE
IRDGVFEEYKCKS
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA