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The map label for this gene is lplA [H]

Identifier: 227828466

GI number: 227828466

Start: 2023933

End: 2024886

Strand: Direct

Name: lplA [H]

Synonym: M1425_2214

Alternate gene names: 227828466

Gene position: 2023933-2024886 (Clockwise)

Preceding gene: 227828462

Following gene: 227828469

Centisome position: 77.58

GC content: 32.29

Gene sequence:

>954_bases
TTGCCATTGTACTGGGATAAAATCTTCAATATTGCTTTCTCAATTTCAACCTCAGCTATAACATCTCCTATTTTAATCTC
ACTTCCTTCTTTTATGTATAATGAGACTATTTCGCCTCCCCAATCTCTTCTTCTAGGCCATATATCTTCAGGGATCTTTA
ACTCAGCTTGCACAAACTATAAATTGATAATGGAAAATAAAAACATAGTGAAGCTTAGGGTATTATTAAAGGAATATCCT
AATGATCCCTATTTGAATGTTGCAATGGACGAAGCACTTTTATTAAATAGTAAAGATGTTCCTACTTTAAGAATATGGAG
AAATGATAAGAGCGTAATTTTAGGAATATTGTCAACTGTTAATGATGAGGTTAATTTAGAAATGATTAAGAAATATGGTG
TAAAATTGGTTAGGAGGATTAGTGGGGGAGGGTCAGTCTTTCATGATATGGGGAACATAAACTATACTTTCATAACAAAC
GGTCAAGGTGGCATAGATTACCTTTACGGCTATTTACTAAAAGGCACGATTAGTGCTATAGAGAGTTTAATAAATGATAG
AGTTGAGGTTTATAACGAAACTGATATTGCGTTTAAGGGTTACAAGATTTCTGGTAATGCTGGATATATAAATGAAGATA
GGTATCTTTTACATGGCACTCTTCTCGTTTCATCTAATTTGGATATATTGCATAAGGTATTAATCATACCTCCTAAAAAT
TACATAAAAAAAGACAATATTAACATGATAAAGTATAAAGTCAGCAATCTCCTAAACTTGGTAAACGGCAATATAAGCTA
TGATGAAGTAGTTGAAGTTTTCATAAGAGGTTTCTCGTCACTCTTACATTGTGATGATTATTTTTTCGACGATATAAGTG
ATGGGGAATTGAAGCATGCAATGAGGCTGGCTAATGAAAAGTACTCCAAAAAAGAGTTCATTTATAAACGTTAA

Upstream 100 bases:

>100_bases
TGTAATTTGAACTTTTTTCTCCATCGTCTAAACCTCTATTAATGCTATAACTGATCCCGGCAATATCTTATCTCCTTCTT
TTACCAAAACTTTAATTACC

Downstream 100 bases:

>100_bases
CATCTCAACTTCTTTGCAACTTCTGCAACTCTTTTACCTAGGAACTTCGCAATCTTAATCTCATTTTCATCTAACTCCTT
CTTATTTCCCAAGTGCGATG

Product: biotin/lipoate A/B protein ligase

Products: NA

Alternate protein names: Lipoate--protein ligase subunit 1 [H]

Number of amino acids: Translated: 317; Mature: 316

Protein sequence:

>317_residues
MPLYWDKIFNIAFSISTSAITSPILISLPSFMYNETISPPQSLLLGHISSGIFNSACTNYKLIMENKNIVKLRVLLKEYP
NDPYLNVAMDEALLLNSKDVPTLRIWRNDKSVILGILSTVNDEVNLEMIKKYGVKLVRRISGGGSVFHDMGNINYTFITN
GQGGIDYLYGYLLKGTISAIESLINDRVEVYNETDIAFKGYKISGNAGYINEDRYLLHGTLLVSSNLDILHKVLIIPPKN
YIKKDNINMIKYKVSNLLNLVNGNISYDEVVEVFIRGFSSLLHCDDYFFDDISDGELKHAMRLANEKYSKKEFIYKR

Sequences:

>Translated_317_residues
MPLYWDKIFNIAFSISTSAITSPILISLPSFMYNETISPPQSLLLGHISSGIFNSACTNYKLIMENKNIVKLRVLLKEYP
NDPYLNVAMDEALLLNSKDVPTLRIWRNDKSVILGILSTVNDEVNLEMIKKYGVKLVRRISGGGSVFHDMGNINYTFITN
GQGGIDYLYGYLLKGTISAIESLINDRVEVYNETDIAFKGYKISGNAGYINEDRYLLHGTLLVSSNLDILHKVLIIPPKN
YIKKDNINMIKYKVSNLLNLVNGNISYDEVVEVFIRGFSSLLHCDDYFFDDISDGELKHAMRLANEKYSKKEFIYKR
>Mature_316_residues
PLYWDKIFNIAFSISTSAITSPILISLPSFMYNETISPPQSLLLGHISSGIFNSACTNYKLIMENKNIVKLRVLLKEYPN
DPYLNVAMDEALLLNSKDVPTLRIWRNDKSVILGILSTVNDEVNLEMIKKYGVKLVRRISGGGSVFHDMGNINYTFITNG
QGGIDYLYGYLLKGTISAIESLINDRVEVYNETDIAFKGYKISGNAGYINEDRYLLHGTLLVSSNLDILHKVLIIPPKNY
IKKDNINMIKYKVSNLLNLVNGNISYDEVVEVFIRGFSSLLHCDDYFFDDISDGELKHAMRLANEKYSKKEFIYKR

Specific function: Lipoate-protein ligase catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes. This subunit can alone only catalyze the lipoat

COG id: COG0095

COG function: function code H; Lipoate-protein ligase A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lplA family [H]

Homologues:

Organism=Homo sapiens, GI7706252, Length=195, Percent_Identity=33.3333333333333, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI21729884, Length=195, Percent_Identity=33.3333333333333, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI21729882, Length=195, Percent_Identity=33.3333333333333, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI21729880, Length=195, Percent_Identity=33.3333333333333, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI21729878, Length=195, Percent_Identity=33.3333333333333, Blast_Score=92, Evalue=6e-19,
Organism=Escherichia coli, GI1790846, Length=205, Percent_Identity=30.2439024390244, Blast_Score=94, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71981909, Length=217, Percent_Identity=24.4239631336406, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6322415, Length=259, Percent_Identity=28.1853281853282, Blast_Score=86, Evalue=9e-18,
Organism=Drosophila melanogaster, GI28573464, Length=158, Percent_Identity=31.6455696202532, Blast_Score=76, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB [H]

EC number: =2.7.7.63 [H]

Molecular weight: Translated: 36029; Mature: 35898

Theoretical pI: Translated: 7.63; Mature: 7.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLYWDKIFNIAFSISTSAITSPILISLPSFMYNETISPPQSLLLGHISSGIFNSACTNY
CCCHHHHHHHHHEEEEHHHHCCCHHHHCCHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCE
KLIMENKNIVKLRVLLKEYPNDPYLNVAMDEALLLNSKDVPTLRIWRNDKSVILGILSTV
EEEEECCCEEEEEEHHHHCCCCCEEEEEECCEEEECCCCCCEEEEEECCCHHHHHHHHHC
NDEVNLEMIKKYGVKLVRRISGGGSVFHDMGNINYTFITNGQGGIDYLYGYLLKGTISAI
CCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHH
ESLINDRVEVYNETDIAFKGYKISGNAGYINEDRYLLHGTLLVSSNLDILHKVLIIPPKN
HHHHHHHHHHCCCCCEEEEEEEEECCCCEECCCCEEEEEEEEEECCHHHEEEEEEECCHH
YIKKDNINMIKYKVSNLLNLVNGNISYDEVVEVFIRGFSSLLHCDDYFFDDISDGELKHA
HCCCCCCCEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHCCCCCCCHHHHH
MRLANEKYSKKEFIYKR
HHHHHHHCCHHHCCCCC
>Mature Secondary Structure 
PLYWDKIFNIAFSISTSAITSPILISLPSFMYNETISPPQSLLLGHISSGIFNSACTNY
CCHHHHHHHHHEEEEHHHHCCCHHHHCCHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCE
KLIMENKNIVKLRVLLKEYPNDPYLNVAMDEALLLNSKDVPTLRIWRNDKSVILGILSTV
EEEEECCCEEEEEEHHHHCCCCCEEEEEECCEEEECCCCCCEEEEEECCCHHHHHHHHHC
NDEVNLEMIKKYGVKLVRRISGGGSVFHDMGNINYTFITNGQGGIDYLYGYLLKGTISAI
CCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHH
ESLINDRVEVYNETDIAFKGYKISGNAGYINEDRYLLHGTLLVSSNLDILHKVLIIPPKN
HHHHHHHHHHCCCCCEEEEEEEEECCCCEECCCCEEEEEEEEEECCHHHEEEEEEECCHH
YIKKDNINMIKYKVSNLLNLVNGNISYDEVVEVFIRGFSSLLHCDDYFFDDISDGELKHA
HCCCCCCCEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHCCCCCCCHHHHH
MRLANEKYSKKEFIYKR
HHHHHHHCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11029001 [H]