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The map label for this gene is glcD [H]

Identifier: 227828461

GI number: 227828461

Start: 2019810

End: 2021213

Strand: Direct

Name: glcD [H]

Synonym: M1425_2209

Alternate gene names: 227828461

Gene position: 2019810-2021213 (Clockwise)

Preceding gene: 227828460

Following gene: 227828462

Centisome position: 77.42

GC content: 37.75

Gene sequence:

>1404_bases
GTGATTGAAAAAATAGCTGAGATAGTAGGCGAGGAGTGGGTAATATCAAGTGATGAAGCCAAGCTGTATTCTTATCCTGC
ATTTATACCTTTGAAAATAGAGCCACCACTAGTTGTTTTACCTGGAAGCGAGGAGGAAGTAATTTCCTTAGTGAATTTTC
TTAACGAGAATAATATAAGATATCTAGTAAGGGGTTCTGGGACAAGTTTAAGCGGGGCAACTACTCCGACACAAGGAGAG
GTCATAATTTCGTTAACAAGACTAAACAGAATTTATTCACTGGATAATTTTGAAATCACCGTAGGACCAGGATTAGCAAA
CTTTCTAATTAATAAAACGTTAAGTAATCCTAACCTTTTCTATGCTCCAGATCCTTCAAGTTATGTAGTATCATCAATAG
GTGGTAATATAAGTCACGACTCTGGGGGAATGCACACTCCAAAATACGGCACCACTTTCGATAGTGTAATAAAATTGAGG
GTTCTTTTAAGTGATGGAAAGGTTGAGGAAATTGGTGGTAATAATTTCTTTGATCCTACATCAATATTTGTAGGTGCAGA
GGGTACTTTAGGGGTTATTTTAAGAGCAACGTTAAGGTTATTTCCAAAACCCGAAGTTAGTAAGACCCTTCTAGCATCGT
TCAATTCAATTGATAATGCCGGAAAAGCCGTTGTAAGTGTTTATAAATCTGGGGTAACACCAGCTGCAATGGAGTTTCTG
GATAAGAATTCCATAATTGCAATAGAGAATACTCAATATAAGGCAAATTACCCTATGGCTGAAGCTATCCTTTTAATAGA
ACTAGACGGATACAGTGCAAATGTAGAAGAGGAAGAGGCTAGAGTAGTAAAGGTAGTAAGGGAGAATAATGGTGAAGTTT
TCATTCCCAAAGATGATGAAGAGAAAAACAGATGGTGGTGGGGCAGAAAAGGAGCTTTTCCGTCTTTTGCCTACTATTCC
CCAGCTTATTTAACACTAGACGCCAATGTTCCTAGATCAACATTGTCAACTATGTTAAGATTCACATATGAAATCAGTAA
GAAATATGGATTGCCAGTTGCAAATAGTTTTCACGCAGGGGATGGCACTTTACATCCGCTAATAGGATTCGATCCTAAAA
ACGTTGATGAGACAATTAATGCAATTAAGGCAGCTGAGGAGATAACGAAACTTGCTATAAAATTAGGTGGGGTACCTAGC
GGGGAACACGGTATCGGAATAGAAAAGATAAAGTTTATGAAGCTTTATTACTCAGAAGACGACTTAACCTTAATGCGAAG
ATTGAAGACAGTATTTGATTCCAAGAGACTTCTAAATCCATGTAAGTTAGTCCCCCCAGCTAAGAATGAAATATGTAATA
CCGTTAATAGGGTGCATACTTACTTGTTGGAGGGAGATATATGA

Upstream 100 bases:

>100_bases
TACTCTTCTACCCGGATTATTACTTTAGTAACTAAGTTTATATAAGGCAAAGAGAAAATAGAAAGGGTATTTTATAAACT
ATCTTGAAGACATTAACCTT

Downstream 100 bases:

>100_bases
TCTCATCTTTGAAATTAACTGAAATGTGTACCCATTGTGGATTTTGTCTAGAAACTTGCCCAACTTATGTTGTAACTAGA
AACGAAGTTCACTCACCAAG

Product: FAD linked oxidase

Products: 2-oxo acid; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 467; Mature: 467

Protein sequence:

>467_residues
MIEKIAEIVGEEWVISSDEAKLYSYPAFIPLKIEPPLVVLPGSEEEVISLVNFLNENNIRYLVRGSGTSLSGATTPTQGE
VIISLTRLNRIYSLDNFEITVGPGLANFLINKTLSNPNLFYAPDPSSYVVSSIGGNISHDSGGMHTPKYGTTFDSVIKLR
VLLSDGKVEEIGGNNFFDPTSIFVGAEGTLGVILRATLRLFPKPEVSKTLLASFNSIDNAGKAVVSVYKSGVTPAAMEFL
DKNSIIAIENTQYKANYPMAEAILLIELDGYSANVEEEEARVVKVVRENNGEVFIPKDDEEKNRWWWGRKGAFPSFAYYS
PAYLTLDANVPRSTLSTMLRFTYEISKKYGLPVANSFHAGDGTLHPLIGFDPKNVDETINAIKAAEEITKLAIKLGGVPS
GEHGIGIEKIKFMKLYYSEDDLTLMRRLKTVFDSKRLLNPCKLVPPAKNEICNTVNRVHTYLLEGDI

Sequences:

>Translated_467_residues
MIEKIAEIVGEEWVISSDEAKLYSYPAFIPLKIEPPLVVLPGSEEEVISLVNFLNENNIRYLVRGSGTSLSGATTPTQGE
VIISLTRLNRIYSLDNFEITVGPGLANFLINKTLSNPNLFYAPDPSSYVVSSIGGNISHDSGGMHTPKYGTTFDSVIKLR
VLLSDGKVEEIGGNNFFDPTSIFVGAEGTLGVILRATLRLFPKPEVSKTLLASFNSIDNAGKAVVSVYKSGVTPAAMEFL
DKNSIIAIENTQYKANYPMAEAILLIELDGYSANVEEEEARVVKVVRENNGEVFIPKDDEEKNRWWWGRKGAFPSFAYYS
PAYLTLDANVPRSTLSTMLRFTYEISKKYGLPVANSFHAGDGTLHPLIGFDPKNVDETINAIKAAEEITKLAIKLGGVPS
GEHGIGIEKIKFMKLYYSEDDLTLMRRLKTVFDSKRLLNPCKLVPPAKNEICNTVNRVHTYLLEGDI
>Mature_467_residues
MIEKIAEIVGEEWVISSDEAKLYSYPAFIPLKIEPPLVVLPGSEEEVISLVNFLNENNIRYLVRGSGTSLSGATTPTQGE
VIISLTRLNRIYSLDNFEITVGPGLANFLINKTLSNPNLFYAPDPSSYVVSSIGGNISHDSGGMHTPKYGTTFDSVIKLR
VLLSDGKVEEIGGNNFFDPTSIFVGAEGTLGVILRATLRLFPKPEVSKTLLASFNSIDNAGKAVVSVYKSGVTPAAMEFL
DKNSIIAIENTQYKANYPMAEAILLIELDGYSANVEEEEARVVKVVRENNGEVFIPKDDEEKNRWWWGRKGAFPSFAYYS
PAYLTLDANVPRSTLSTMLRFTYEISKKYGLPVANSFHAGDGTLHPLIGFDPKNVDETINAIKAAEEITKLAIKLGGVPS
GEHGIGIEKIKFMKLYYSEDDLTLMRRLKTVFDSKRLLNPCKLVPPAKNEICNTVNRVHTYLLEGDI

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI37595756, Length=428, Percent_Identity=31.3084112149533, Blast_Score=196, Evalue=5e-50,
Organism=Homo sapiens, GI37595754, Length=451, Percent_Identity=29.9334811529933, Blast_Score=188, Evalue=1e-47,
Organism=Homo sapiens, GI119964728, Length=433, Percent_Identity=22.6327944572748, Blast_Score=102, Evalue=6e-22,
Organism=Homo sapiens, GI4501993, Length=249, Percent_Identity=26.9076305220884, Blast_Score=85, Evalue=2e-16,
Organism=Escherichia coli, GI1789351, Length=460, Percent_Identity=37.1739130434783, Blast_Score=310, Evalue=1e-85,
Organism=Escherichia coli, GI48994907, Length=496, Percent_Identity=22.1774193548387, Blast_Score=75, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI71992373, Length=426, Percent_Identity=26.7605633802817, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI71992381, Length=424, Percent_Identity=26.1792452830189, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI71992392, Length=383, Percent_Identity=25.8485639686684, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17534361, Length=442, Percent_Identity=25.5656108597285, Blast_Score=108, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI17556096, Length=469, Percent_Identity=21.1087420042644, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6320027, Length=439, Percent_Identity=29.1571753986333, Blast_Score=169, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6320023, Length=471, Percent_Identity=23.9915074309979, Blast_Score=123, Evalue=8e-29,
Organism=Saccharomyces cerevisiae, GI6320764, Length=438, Percent_Identity=24.8858447488584, Blast_Score=112, Evalue=1e-25,
Organism=Drosophila melanogaster, GI18921117, Length=463, Percent_Identity=25.7019438444924, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI24639277, Length=463, Percent_Identity=25.7019438444924, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI24639275, Length=463, Percent_Identity=25.7019438444924, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI24653753, Length=467, Percent_Identity=23.5546038543897, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR004490
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: 1.1.3.15

Molecular weight: Translated: 51491; Mature: 51491

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEKIAEIVGEEWVISSDEAKLYSYPAFIPLKIEPPLVVLPGSEEEVISLVNFLNENNIR
CHHHHHHHCCCCEEEECCCCEEEECCCEEEEEECCCEEEECCCHHHHHHHHHHHCCCCEE
YLVRGSGTSLSGATTPTQGEVIISLTRLNRIYSLDNFEITVGPGLANFLINKTLSNPNLF
EEEEECCCCCCCCCCCCCCCEEEEEEHHHHHEECCCEEEEECCCHHHHHHHHCCCCCCEE
YAPDPSSYVVSSIGGNISHDSGGMHTPKYGTTFDSVIKLRVLLSDGKVEEIGGNNFFDPT
ECCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHEEEEEEECCCCEEECCCCCCCCCC
SIFVGAEGTLGVILRATLRLFPKPEVSKTLLASFNSIDNAGKAVVSVYKSGVTPAAMEFL
EEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHH
DKNSIIAIENTQYKANYPMAEAILLIELDGYSANVEEEEARVVKVVRENNGEVFIPKDDE
CCCCEEEEECCEEECCCCHHHEEEEEEECCCCCCCCHHHHHEEEEEECCCCEEEECCCCC
EKNRWWWGRKGAFPSFAYYSPAYLTLDANVPRSTLSTMLRFTYEISKKYGLPVANSFHAG
CCCCEECCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
DGTLHPLIGFDPKNVDETINAIKAAEEITKLAIKLGGVPSGEHGIGIEKIKFMKLYYSED
CCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHEEEEEEEEECCC
DLTLMRRLKTVFDSKRLLNPCKLVPPAKNEICNTVNRVHTYLLEGDI
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHEEHEEECCC
>Mature Secondary Structure
MIEKIAEIVGEEWVISSDEAKLYSYPAFIPLKIEPPLVVLPGSEEEVISLVNFLNENNIR
CHHHHHHHCCCCEEEECCCCEEEECCCEEEEEECCCEEEECCCHHHHHHHHHHHCCCCEE
YLVRGSGTSLSGATTPTQGEVIISLTRLNRIYSLDNFEITVGPGLANFLINKTLSNPNLF
EEEEECCCCCCCCCCCCCCCEEEEEEHHHHHEECCCEEEEECCCHHHHHHHHCCCCCCEE
YAPDPSSYVVSSIGGNISHDSGGMHTPKYGTTFDSVIKLRVLLSDGKVEEIGGNNFFDPT
ECCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHEEEEEEECCCCEEECCCCCCCCCC
SIFVGAEGTLGVILRATLRLFPKPEVSKTLLASFNSIDNAGKAVVSVYKSGVTPAAMEFL
EEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHH
DKNSIIAIENTQYKANYPMAEAILLIELDGYSANVEEEEARVVKVVRENNGEVFIPKDDE
CCCCEEEEECCEEECCCCHHHEEEEEEECCCCCCCCHHHHHEEEEEECCCCEEEECCCCC
EKNRWWWGRKGAFPSFAYYSPAYLTLDANVPRSTLSTMLRFTYEISKKYGLPVANSFHAG
CCCCEECCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
DGTLHPLIGFDPKNVDETINAIKAAEEITKLAIKLGGVPSGEHGIGIEKIKFMKLYYSED
CCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHEEEEEEEEECCC
DLTLMRRLKTVFDSKRLLNPCKLVPPAKNEICNTVNRVHTYLLEGDI
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHEEHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-hydroxy acid; O2

Specific reaction: (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]