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The map label for this gene is yoaE [H]

Identifier: 227828423

GI number: 227828423

Start: 1987368

End: 1989110

Strand: Direct

Name: yoaE [H]

Synonym: M1425_2167

Alternate gene names: 227828423

Gene position: 1987368-1989110 (Clockwise)

Preceding gene: 227828422

Following gene: 227828427

Centisome position: 76.18

GC content: 35.92

Gene sequence:

>1743_bases
ATGGTTCACGCTTGTACGCGAGATTGTTATGACACTTGTATATTTGACGATACCCATAAACCACTCGATATATTTCCTTT
TAATGGGTTTACATGTTCTAGGGGTATAGCGGATTTAAAGAGGAACTCGTTGAATAGAATTGATAATGTTTATATAGAAG
GAAAGCCAAATAGTATTGATAAGGCATTAGATTTAATAGTTAAGGAGATAAGAAAACGGAAAAAAGAGGAAATTCTTCAT
GTCGACTATGATGGAAACCAAGGTCTATTGACGTGGTATTTTCCGGCAAGACTATGGAATGTAATAGGTGCTGCATCAAC
TGACTACTCAATATGCAGTTCAGAAGGTCATGAGGCAATTAAGTTACATTATCGTAATTCAATAGGTGCTTTACCAGAGG
ATTTCCCTAAATATGATTCATTTGCCATATGGGGAAGTGAATTAGTATTTAGTTTCATTCATGGTTGGAACTTGATTAAA
GATAAGTATAAGGTAACGATCGATGTTAGAATATCTGAAACAGCTAAAAGGAGTAATAAATACTACATTGTGAAAGCTGG
AAGTGACGCGTATTTGGCTATAGGGATCATGAAAAAACTATTTGAGAGAAACTGGGCTGATTTATCACTCCTAGATGATC
CAGAAGAATTAAGGGAATATGTCTTTTCATTTAAGGATGAAGAAATCGAGGAGGCCACTGGTTTGGATTCTTCTAAAATT
AATGAGTTAGCTGAAATGTATCATTATAGAAGACCTTTAACGATTATTGGATTTGCGCTAGGCAGGAGCATAAACGGTGG
TGACGCTATTTCACTAATCTCATTAATACCTGCACTTATTGGGATGAAAAAGGGGTTTTTCTATGCCAATTCTCAAGGAC
TTGGAATAGATTTTAGATATTTAAGAGGGCTGCACAGGTACTCTCCTTCAAGAATTGTAGGCATGGCAGAAGTTGGCAAG
GAGGTTGAAGAGGGTAGGATAACTTTTATGTTTGTTTGGAACTCTAATCCCTTACATTCTTTGCCAATGTCTGATAGAAT
ATATGAGGCTGTAAAAGAGGGTAGATTATTTCTTGTTGTGCATGATCCTTATTGGTCTGAGACTACGAAGATAGCTAATG
TAGTGTTACCTGCTCCTACGTATCTTGAGAAAGAGGATGTCGTCTACAGTTATTGGCACAATTATCTAGTTTACAATAAA
CCAATCCTACCTAAAAGAGGCTTTACTGAGGTCGAATTAATGAGAGTGTTGGCTGTAAAACTAGAAATTGACGACGACGT
TATATCTGAGGACGAGTGGCTGGCTATAGGTAAAGCTACTGGGGTTAATGTAACAGAGCTAAAGACTAAAGGATTCGTAA
AGTTATTACCTAAATATCCAGAAGGTAAGGTGAGAGTTAAACCACTTCCAGATAATCTCAAGAAGCCTAATGGAAATATC
ATCGTTTTTTCCTCTCATCCTAATTATACTAATAGTCAATTTAAGGAAATTTATGGTGATAAGAAGGGCATTGTGTATAA
TTCAGAATTTGAGGGAATAGGTTATTTATCAACAAACTACGGTAAGGTTAAGGTTATGTTTAAGAGGGATGCTTCAATCC
CGAGAGGAGTTTTCTTCATTCATAAATCTTTCTTGTTTGATCTTGAGGGTAAGCCTATTAATAGTATTATAGGTTTTGCT
AAGGGAAAGTACGGTAATACTCCAATTATTAATATAGATTACGTGGAGACTATAAAAGCTTGA

Upstream 100 bases:

>100_bases
TTCTGCTAAGGTTTTCAGCTTAAAGGAATTTCGTCACATTTCAGCTACGGTCGCTCTCTTCACTCAAACTTAAATTCTGT
ATTTTCCCATTATAAAAATA

Downstream 100 bases:

>100_bases
AAGTTGACGATTTTAGTATTTTCACTAAGGGCAATTATGAAGAGCGTTGTCATAACGAGGAACATCAGTGTCGCTGTTAA
AACTTCTTGGTCCAATGGCC

Product: hypothetical protein

Products: CO2; NADH; H2 [C]

Alternate protein names: NA

Number of amino acids: Translated: 580; Mature: 580

Protein sequence:

>580_residues
MVHACTRDCYDTCIFDDTHKPLDIFPFNGFTCSRGIADLKRNSLNRIDNVYIEGKPNSIDKALDLIVKEIRKRKKEEILH
VDYDGNQGLLTWYFPARLWNVIGAASTDYSICSSEGHEAIKLHYRNSIGALPEDFPKYDSFAIWGSELVFSFIHGWNLIK
DKYKVTIDVRISETAKRSNKYYIVKAGSDAYLAIGIMKKLFERNWADLSLLDDPEELREYVFSFKDEEIEEATGLDSSKI
NELAEMYHYRRPLTIIGFALGRSINGGDAISLISLIPALIGMKKGFFYANSQGLGIDFRYLRGLHRYSPSRIVGMAEVGK
EVEEGRITFMFVWNSNPLHSLPMSDRIYEAVKEGRLFLVVHDPYWSETTKIANVVLPAPTYLEKEDVVYSYWHNYLVYNK
PILPKRGFTEVELMRVLAVKLEIDDDVISEDEWLAIGKATGVNVTELKTKGFVKLLPKYPEGKVRVKPLPDNLKKPNGNI
IVFSSHPNYTNSQFKEIYGDKKGIVYNSEFEGIGYLSTNYGKVKVMFKRDASIPRGVFFIHKSFLFDLEGKPINSIIGFA
KGKYGNTPIINIDYVETIKA

Sequences:

>Translated_580_residues
MVHACTRDCYDTCIFDDTHKPLDIFPFNGFTCSRGIADLKRNSLNRIDNVYIEGKPNSIDKALDLIVKEIRKRKKEEILH
VDYDGNQGLLTWYFPARLWNVIGAASTDYSICSSEGHEAIKLHYRNSIGALPEDFPKYDSFAIWGSELVFSFIHGWNLIK
DKYKVTIDVRISETAKRSNKYYIVKAGSDAYLAIGIMKKLFERNWADLSLLDDPEELREYVFSFKDEEIEEATGLDSSKI
NELAEMYHYRRPLTIIGFALGRSINGGDAISLISLIPALIGMKKGFFYANSQGLGIDFRYLRGLHRYSPSRIVGMAEVGK
EVEEGRITFMFVWNSNPLHSLPMSDRIYEAVKEGRLFLVVHDPYWSETTKIANVVLPAPTYLEKEDVVYSYWHNYLVYNK
PILPKRGFTEVELMRVLAVKLEIDDDVISEDEWLAIGKATGVNVTELKTKGFVKLLPKYPEGKVRVKPLPDNLKKPNGNI
IVFSSHPNYTNSQFKEIYGDKKGIVYNSEFEGIGYLSTNYGKVKVMFKRDASIPRGVFFIHKSFLFDLEGKPINSIIGFA
KGKYGNTPIINIDYVETIKA
>Mature_580_residues
MVHACTRDCYDTCIFDDTHKPLDIFPFNGFTCSRGIADLKRNSLNRIDNVYIEGKPNSIDKALDLIVKEIRKRKKEEILH
VDYDGNQGLLTWYFPARLWNVIGAASTDYSICSSEGHEAIKLHYRNSIGALPEDFPKYDSFAIWGSELVFSFIHGWNLIK
DKYKVTIDVRISETAKRSNKYYIVKAGSDAYLAIGIMKKLFERNWADLSLLDDPEELREYVFSFKDEEIEEATGLDSSKI
NELAEMYHYRRPLTIIGFALGRSINGGDAISLISLIPALIGMKKGFFYANSQGLGIDFRYLRGLHRYSPSRIVGMAEVGK
EVEEGRITFMFVWNSNPLHSLPMSDRIYEAVKEGRLFLVVHDPYWSETTKIANVVLPAPTYLEKEDVVYSYWHNYLVYNK
PILPKRGFTEVELMRVLAVKLEIDDDVISEDEWLAIGKATGVNVTELKTKGFVKLLPKYPEGKVRVKPLPDNLKKPNGNI
IVFSSHPNYTNSQFKEIYGDKKGIVYNSEFEGIGYLSTNYGKVKVMFKRDASIPRGVFFIHKSFLFDLEGKPINSIIGFA
KGKYGNTPIINIDYVETIKA

Specific function: Decomposes Formic Acid To Hydrogen And Carbon Dioxide Under Anaerobic Conditions In The Absence Of Exogenous Electron Acceptors. [C]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: 1.2.1.2 [C]

Molecular weight: Translated: 66221; Mature: 66221

Theoretical pI: Translated: 7.65; Mature: 7.65

Prosite motif: PS00019 ACTININ_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVHACTRDCYDTCIFDDTHKPLDIFPFNGFTCSRGIADLKRNSLNRIDNVYIEGKPNSID
CCCCCCHHHHHHHEECCCCCCEEEECCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCHHH
KALDLIVKEIRKRKKEEILHVDYDGNQGLLTWYFPARLWNVIGAASTDYSICSSEGHEAI
HHHHHHHHHHHHHHHHCEEEEEECCCCCEEEEEEHHHHHHHHCCCCCCCHHHCCCCCCEE
KLHYRNSIGALPEDFPKYDSFAIWGSELVFSFIHGWNLIKDKYKVTIDVRISETAKRSNK
EEEECCCCCCCCCCCCCCCCEEEEHHHHHHHHHHCCCEECCCEEEEEEEEEEHHHHCCCC
YYIVKAGSDAYLAIGIMKKLFERNWADLSLLDDPEELREYVFSFKDEEIEEATGLDSSKI
EEEEEECCCCEEHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHCCCHHHHHHCCCCHHHH
NELAEMYHYRRPLTIIGFALGRSINGGDAISLISLIPALIGMKKGFFYANSQGLGIDFRY
HHHHHHHHHCCCHHEEEHHHCCCCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCEEHHH
LRGLHRYSPSRIVGMAEVGKEVEEGRITFMFVWNSNPLHSLPMSDRIYEAVKEGRLFLVV
HHHHHHCCCHHEEEHHHHCCHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHHCCCEEEEE
HDPYWSETTKIANVVLPAPTYLEKEDVVYSYWHNYLVYNKPILPKRGFTEVELMRVLAVK
ECCCCCCCHHHEEEEECCCCCCCCCCCHHHHHCCEEEECCCCCCCCCCHHHHHHHHHHEE
LEIDDDVISEDEWLAIGKATGVNVTELKTKGFVKLLPKYPEGKVRVKPLPDNLKKPNGNI
EECCCCCCCCCCEEEEECCCCCCCEEECCCCHHEECCCCCCCCEEEEECCCCCCCCCCCE
IVFSSHPNYTNSQFKEIYGDKKGIVYNSEFEGIGYLSTNYGKVKVMFKRDASIPRGVFFI
EEEECCCCCCCHHHHHHHCCCCCEEECCCCCCEEEEECCCCEEEEEEECCCCCCCCEEEE
HKSFLFDLEGKPINSIIGFAKGKYGNTPIINIDYVETIKA
EEEEEEECCCCCHHHHHHHHCCCCCCCCEEEEEHHHCCCC
>Mature Secondary Structure
MVHACTRDCYDTCIFDDTHKPLDIFPFNGFTCSRGIADLKRNSLNRIDNVYIEGKPNSID
CCCCCCHHHHHHHEECCCCCCEEEECCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCHHH
KALDLIVKEIRKRKKEEILHVDYDGNQGLLTWYFPARLWNVIGAASTDYSICSSEGHEAI
HHHHHHHHHHHHHHHHCEEEEEECCCCCEEEEEEHHHHHHHHCCCCCCCHHHCCCCCCEE
KLHYRNSIGALPEDFPKYDSFAIWGSELVFSFIHGWNLIKDKYKVTIDVRISETAKRSNK
EEEECCCCCCCCCCCCCCCCEEEEHHHHHHHHHHCCCEECCCEEEEEEEEEEHHHHCCCC
YYIVKAGSDAYLAIGIMKKLFERNWADLSLLDDPEELREYVFSFKDEEIEEATGLDSSKI
EEEEEECCCCEEHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHCCCHHHHHHCCCCHHHH
NELAEMYHYRRPLTIIGFALGRSINGGDAISLISLIPALIGMKKGFFYANSQGLGIDFRY
HHHHHHHHHCCCHHEEEHHHCCCCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCEEHHH
LRGLHRYSPSRIVGMAEVGKEVEEGRITFMFVWNSNPLHSLPMSDRIYEAVKEGRLFLVV
HHHHHHCCCHHEEEHHHHCCHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHHCCCEEEEE
HDPYWSETTKIANVVLPAPTYLEKEDVVYSYWHNYLVYNKPILPKRGFTEVELMRVLAVK
ECCCCCCCHHHEEEEECCCCCCCCCCCHHHHHCCEEEECCCCCCCCCCHHHHHHHHHHEE
LEIDDDVISEDEWLAIGKATGVNVTELKTKGFVKLLPKYPEGKVRVKPLPDNLKKPNGNI
EECCCCCCCCCCEEEEECCCCCCCEEECCCCHHEECCCCCCCCEEEEECCCCCCCCCCCE
IVFSSHPNYTNSQFKEIYGDKKGIVYNSEFEGIGYLSTNYGKVKVMFKRDASIPRGVFFI
EEEECCCCCCCHHHHHHHCCCCCEEECCCCCCEEEEECCCCEEEEEEECCCCCCCCEEEE
HKSFLFDLEGKPINSIIGFAKGKYGNTPIINIDYVETIKA
EEEEEEECCCCCHHHHHHHHCCCCCCCCEEEEEHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: Fe; Mo; Se [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.032 {NAD+}} 0.54 {deamino-NAD+}} 0.19 {3-pyridinecarboxaldehyde-NAD+}} 1.16 {3-acetylpyridine-NAD+}} 0.17 {thio-NAD+}} [C]

Substrates: Formate; NAD(+) [C]

Specific reaction: Formate + NAD(+) = CO2 + NADH. formate = CO2 + H2 [C]

General reaction: Redox reaction [C]

Inhibitor: Azide; Br-; Cl-; CN-; F-; HCO3-; HCOS-; NO2-; NO3-; OCN-; SCN- [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]