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The map label for this gene is dgoD [H]

Identifier: 227828418

GI number: 227828418

Start: 1981710

End: 1982897

Strand: Direct

Name: dgoD [H]

Synonym: M1425_2162

Alternate gene names: 227828418

Gene position: 1981710-1982897 (Clockwise)

Preceding gene: 227828416

Following gene: 227828419

Centisome position: 75.96

GC content: 38.22

Gene sequence:

>1188_bases
ATGAAAATCAGAGAAATAGAACCAATAGTACTTACCTCGAAAGAGAAAGGTAGTGCAACTTGGGCGTCTACGATGATTAT
TGTAAGGATAATAACGGAAAATGGAGCTGTAGGATATGGCGAGGCTGTACCAACACTAAGAGTTATATCCGTATACAATG
CTATTAAACAAGTTGCTAAGGGTTATATAGGAAAAGAAGTAGAAGAGGTTGAGAAGAACTATCATGAATGGTATAAACAA
GACTTCTACTTAGCTAGGTCTTTTGAATCAACAACTGCAGTAAGTGCAATTGATATAGCCTCTTGGGATATAATAGGAAA
GGAGCTTGGAGCCCCAATCTACAAGTTATTAGGTGGAAAAGTTAGGAATAGGGTACCAGTCTATGCAAATGGATGGTATC
AAGACTGTGTAACTCCAGAGGACTTTGCAGAAAAGGCAAAAGAGATTGTAAAAACGGGATATAAGGCTCTAAAATTTGAT
CCATTTGGACCCTACTACGATTGGATAGACGAGAGAGGTTTAAGAGAAGCTGAGGAGAGAGTAAGGGCTGTTAGGGAGGC
AGTTGGTAACAACGTCGATATTTTAATAGAGCATCACGGTAGGTTTAATGCAAATTCCGCAATAATGATAGCGAAAAGGT
TAGAAAAATACAATCCAGGATTTATGGAGGAACCAGTACATCATGAGGATATCATCGGTTTAAGGAAGTATAGAGCTAAT
ACTCATTTGAGGATTGCATTGGGAGAAAGGCTGCTAAGTGAAAAGGAAGCCGCGTTTTACGTTGAGGAAGGTCTTGTAAA
CATATTGCAACCAGATTTAACTAATATAGGCGGTGTGACAGTAGGTAAGAGCGTTATAAAGATAGCTGAAGCTAATGACG
TAGAAGTGGCTTTCCATAACGCCTTTGGTTCAATTCAGAATGCTGTCGAAATACAACTAAGTGCAGTTACACAGAACTTA
TATTTGCTTGAAAATTTCTATGATTGGTTCCCTCAATGGAAAAGGGATTTAGTGTATAATGAAACACCAGTTGAAGGAGG
TCATGTTAAGGTTCCAGATAAACCCGGTATAGGTGTTTCAATTAATGAAAAAATAATAGAGCAATTAAGAGCTGAACCAA
TACCATTAGATGTAATTGAAGAACCGGTATGGGTTGTTAAAGGAACTTGGAAGAATTACGGTGTCTGA

Upstream 100 bases:

>100_bases
ATAAAATGATTCGGATAATATAACCTATAAATTTTATTAAATCCTTATCACTGAGTCTTGCCATTATGCAAGTTTATATT
TGTTGTCCAAATTATAGTTT

Downstream 100 bases:

>100_bases
AAATGCCAGAGATCGTAACTCCAATCATAACTCCATTTACTAAAGATAATAGAATAGATAAGGATAAGTTAACGCTACAT
GCGGAGAATCTCATTAGGAA

Product: mandelate racemase/muconate lactonizing protein

Products: NA

Alternate protein names: GalD [H]

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MKIREIEPIVLTSKEKGSATWASTMIIVRIITENGAVGYGEAVPTLRVISVYNAIKQVAKGYIGKEVEEVEKNYHEWYKQ
DFYLARSFESTTAVSAIDIASWDIIGKELGAPIYKLLGGKVRNRVPVYANGWYQDCVTPEDFAEKAKEIVKTGYKALKFD
PFGPYYDWIDERGLREAEERVRAVREAVGNNVDILIEHHGRFNANSAIMIAKRLEKYNPGFMEEPVHHEDIIGLRKYRAN
THLRIALGERLLSEKEAAFYVEEGLVNILQPDLTNIGGVTVGKSVIKIAEANDVEVAFHNAFGSIQNAVEIQLSAVTQNL
YLLENFYDWFPQWKRDLVYNETPVEGGHVKVPDKPGIGVSINEKIIEQLRAEPIPLDVIEEPVWVVKGTWKNYGV

Sequences:

>Translated_395_residues
MKIREIEPIVLTSKEKGSATWASTMIIVRIITENGAVGYGEAVPTLRVISVYNAIKQVAKGYIGKEVEEVEKNYHEWYKQ
DFYLARSFESTTAVSAIDIASWDIIGKELGAPIYKLLGGKVRNRVPVYANGWYQDCVTPEDFAEKAKEIVKTGYKALKFD
PFGPYYDWIDERGLREAEERVRAVREAVGNNVDILIEHHGRFNANSAIMIAKRLEKYNPGFMEEPVHHEDIIGLRKYRAN
THLRIALGERLLSEKEAAFYVEEGLVNILQPDLTNIGGVTVGKSVIKIAEANDVEVAFHNAFGSIQNAVEIQLSAVTQNL
YLLENFYDWFPQWKRDLVYNETPVEGGHVKVPDKPGIGVSINEKIIEQLRAEPIPLDVIEEPVWVVKGTWKNYGV
>Mature_395_residues
MKIREIEPIVLTSKEKGSATWASTMIIVRIITENGAVGYGEAVPTLRVISVYNAIKQVAKGYIGKEVEEVEKNYHEWYKQ
DFYLARSFESTTAVSAIDIASWDIIGKELGAPIYKLLGGKVRNRVPVYANGWYQDCVTPEDFAEKAKEIVKTGYKALKFD
PFGPYYDWIDERGLREAEERVRAVREAVGNNVDILIEHHGRFNANSAIMIAKRLEKYNPGFMEEPVHHEDIIGLRKYRAN
THLRIALGERLLSEKEAAFYVEEGLVNILQPDLTNIGGVTVGKSVIKIAEANDVEVAFHNAFGSIQNAVEIQLSAVTQNL
YLLENFYDWFPQWKRDLVYNETPVEGGHVKVPDKPGIGVSINEKIIEQLRAEPIPLDVIEEPVWVVKGTWKNYGV

Specific function: Catalyzes the dehydration of D-galactonate to 2-keto-3- deoxy-D-galactonate [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GalD subfamily [H]

Homologues:

Organism=Escherichia coli, GI48994953, Length=355, Percent_Identity=32.3943661971831, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI1787864, Length=380, Percent_Identity=26.5789473684211, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI226510960, Length=345, Percent_Identity=26.0869565217391, Blast_Score=96, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =4.2.1.6 [H]

Molecular weight: Translated: 44668; Mature: 44668

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIREIEPIVLTSKEKGSATWASTMIIVRIITENGAVGYGEAVPTLRVISVYNAIKQVAK
CCCCCCCCEEEECCCCCCCHHHHHEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHH
GYIGKEVEEVEKNYHEWYKQDFYLARSFESTTAVSAIDIASWDIIGKELGAPIYKLLGGK
HHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHEEEEHHHHHHHHHHHCCHHHHHHCCH
VRNRVPVYANGWYQDCVTPEDFAEKAKEIVKTGYKALKFDPFGPYYDWIDERGLREAEER
HHCCCCEEECCCHHCCCCHHHHHHHHHHHHHCCCHHEEECCCCCHHHHHHHCCHHHHHHH
VRAVREAVGNNVDILIEHHGRFNANSAIMIAKRLEKYNPGFMEEPVHHEDIIGLRKYRAN
HHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCC
THLRIALGERLLSEKEAAFYVEEGLVNILQPDLTNIGGVTVGKSVIKIAEANDVEVAFHN
CEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCHHHHHHHHEEECCCCEEEEEEH
AFGSIQNAVEIQLSAVTQNLYLLENFYDWFPQWKRDLVYNETPVEGGHVKVPDKPGIGVS
HHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCC
INEKIIEQLRAEPIPLDVIEEPVWVVKGTWKNYGV
CCHHHHHHHCCCCCCHHHHCCCEEEEEECCCCCCC
>Mature Secondary Structure
MKIREIEPIVLTSKEKGSATWASTMIIVRIITENGAVGYGEAVPTLRVISVYNAIKQVAK
CCCCCCCCEEEECCCCCCCHHHHHEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHH
GYIGKEVEEVEKNYHEWYKQDFYLARSFESTTAVSAIDIASWDIIGKELGAPIYKLLGGK
HHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHEEEEHHHHHHHHHHHCCHHHHHHCCH
VRNRVPVYANGWYQDCVTPEDFAEKAKEIVKTGYKALKFDPFGPYYDWIDERGLREAEER
HHCCCCEEECCCHHCCCCHHHHHHHHHHHHHCCCHHEEECCCCCHHHHHHHCCHHHHHHH
VRAVREAVGNNVDILIEHHGRFNANSAIMIAKRLEKYNPGFMEEPVHHEDIIGLRKYRAN
HHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCC
THLRIALGERLLSEKEAAFYVEEGLVNILQPDLTNIGGVTVGKSVIKIAEANDVEVAFHN
CEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCHHHHHHHHEEECCCCEEEEEEH
AFGSIQNAVEIQLSAVTQNLYLLENFYDWFPQWKRDLVYNETPVEGGHVKVPDKPGIGVS
HHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCC
INEKIIEQLRAEPIPLDVIEEPVWVVKGTWKNYGV
CCHHHHHHHCCCCCCHHHHCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA