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The map label for this gene is ypfI [C]

Identifier: 227828403

GI number: 227828403

Start: 1963926

End: 1966238

Strand: Direct

Name: ypfI [C]

Synonym: M1425_2146

Alternate gene names: 227828403

Gene position: 1963926-1966238 (Clockwise)

Preceding gene: 227828402

Following gene: 227828405

Centisome position: 75.28

GC content: 34.28

Gene sequence:

>2313_bases
ATGGTAAATAAAGAACAGTTTTATGATCAAATAAGAAAGGCATTAGAAGACGGTAATATTAGATACTATAGGAACTTAGT
TTACATAGAGAGGGAGGATTATCTAGATTATGTGAAGGAAATTATAAATCTATTTCTTAAATTTAAACCAGACCCTTCAG
TAGCGTATGGTTTCGTACCTTGGGCGAGTGGTTCTAAAGAGAGGATGAAGACTATAAAAGAATATTTCTTGAAATTTAAT
GATATCGATTACGCTAATGCTGAATATTACCTAGGTAATACTTATGATTTGGTCATATTGGATACTGTAGATAACTTTCA
GCCAATTAATATAGGTAGACTTGTTGATCTTGCTAGAGGTGGAGGTTTAATAATAATTTATACAAATAATCTAACGAAAG
ATAAGGCTTTTAGAACTTCAATACTGAGGAACGGTTTAGTTTTAGACGAATATGAGAAGAGGTTCAAAAGAAAATTGTAT
GAGCATGAAGGGATATTTATCGTTGATGCTAACGGATATGTTTCTAAGCCATTTTCCGGTAACATAATGCTAAAATCAGA
GAAGAAAGTACCAAGAAATCCCGTAATGCCAAGAGAAATTCACGAATTATCACTAAGTGAGGATCAAAACAGAGTTATAG
AGAATTTCACTTATTTATTAAGTGGAGGGCAAAGAGCTTTAGTTTTAACAGCTGCTAGGGGCAGAGGAAAAAGTGCAGCT
ACTGGATTATCTATAGCGGGGCTAATAGAAAAGCTTAGGGAAAGAAAAGGGCAAAGCGTTAGAGTAATAGTTACTGCTCC
ATCAATAGCTAACGCATCTCAAGTAATGTCGTTTGCTAAATTAGGTTTAGAAGTTTTAGGTGAAGAGCTGTCAGTAAAAG
TTAGTGATACAGGGCATATAAAGACATTAAGAGGTGATTATTTCAAGGTAGAGTATGTTCCACCTGACGCAGCGGTTGAA
GATGATGGTGAGCTTTTAATCGTAGATGAAGCCGCAGCCTTAGGCATCAATTATATAGATTTAGCACTAAGAGCTTGGAA
AAAGGTGGCGCTAGTAACTACAGTACATGGATATGAGGGTTCTAACAAGGCTTTTCTTAGATATTTAAGAAGATTAATTG
AAAGTAAAAGGATTAGGGTTAAATGGATAAATATAGAACAGCCTTTAAGATATGCAAATGGAGATCCAATAGAAAAGTGG
CTCTACGACGCGTTATTACTTGACGCAGAACCTTCAGAACCACAATATCTTAATCATACAATGATTTATGAAGATGTGGA
TAAATCTGAACTAGCCAACGATGATATCAAATTAAGAGCAATTTACGGAATAATGGTAACAGCTCACTACAAAAATAATC
CAGATGACTTAATGATAATGTTGGATGGCGTTCATCATAAGATAAAGGCCATACGGATAGGGGAGAACTCATACGTTGCG
GCTTGTCAAATAGCTGAAGAAGGTGAACTTTCTGACAATATGGTAGATATTGCTTTAAAAGGTGGTACGTTTGAGGGTGA
TCTAATACCTGACAGAATTATAAAGCACGTTAGGATTAAGGATTTTGCTAGATTAAGAGGGTGGAGGATTGTTAGAATTG
CCGTTGCTTCAGAACTTCAAGATAAGGGATTTGGTAGTGAGCTATTAAAAATGATATATGAAGAGGCTAAGGATAAAGGA
ATGGACTGGGTAGGTTCTTCTTTTATGTCTGATCAAAAAGTTCTAAATTTCTGGATAAAGAACGGCTTTGCCCCAGTTCA
TATATCACCAAAGAAAAATGAGAAATTAGGTGATTACCCAGTTGTTGTTATTAGACCTATAAGTGATATAGCAACGAAGA
TTGTAAAAATATCTGTGTATATGTTAAAAGAAAAGCTCCTTAACACCTTACACGATGTGTACTTTAATATGAACCCTGAA
GTAGTTAGGTTAATATTAAAAGGAAGTAGTATTGCTCATAAGACCGTTGATGTGAATCCAATATTATTAGATAAAACTAT
CTCATTTCTTCAAGGTGTAAGTCCATATGAATCCTCAGCTGATGGAATACATATGTTAACATTAAAGTATTTCTGGGATG
GAGAAAGAGATTGGGGTTTGACTCAAGATGAAGAATTGGTGTTAATTGCTAAAGTTATTCAAGGTAAGCCATGGAGTTAT
GTGTCTACTATACTTAATAGTAATAGGACACATATATATGAATTAATATATTCTGCTATATCAAAAATAATGCAAAAATA
TTATAACTTAACCGCTGATAGTAAGGTAGGTCTAACATTAAAGGATGTGATGAGTTCACAACAATATGAATAG

Upstream 100 bases:

>100_bases
AGAGGAATTAGCGGATGTTATAGCTTGGACAGTATCGATAGCTAACTTAAAAGGTATAGACATAGAAGAAGCTTTAAAGA
AGAAGTATAAATTGTAAGCA

Downstream 100 bases:

>100_bases
GTGAATGGATGCACCTCAACTGATGCTATTCAGTATAATTGCCACTACCACGAGAATCGAGAATAGTTGATGTAATCCAA
TAGACATGCCGGGCTTTAAT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 770; Mature: 770

Protein sequence:

>770_residues
MVNKEQFYDQIRKALEDGNIRYYRNLVYIEREDYLDYVKEIINLFLKFKPDPSVAYGFVPWASGSKERMKTIKEYFLKFN
DIDYANAEYYLGNTYDLVILDTVDNFQPINIGRLVDLARGGGLIIIYTNNLTKDKAFRTSILRNGLVLDEYEKRFKRKLY
EHEGIFIVDANGYVSKPFSGNIMLKSEKKVPRNPVMPREIHELSLSEDQNRVIENFTYLLSGGQRALVLTAARGRGKSAA
TGLSIAGLIEKLRERKGQSVRVIVTAPSIANASQVMSFAKLGLEVLGEELSVKVSDTGHIKTLRGDYFKVEYVPPDAAVE
DDGELLIVDEAAALGINYIDLALRAWKKVALVTTVHGYEGSNKAFLRYLRRLIESKRIRVKWINIEQPLRYANGDPIEKW
LYDALLLDAEPSEPQYLNHTMIYEDVDKSELANDDIKLRAIYGIMVTAHYKNNPDDLMIMLDGVHHKIKAIRIGENSYVA
ACQIAEEGELSDNMVDIALKGGTFEGDLIPDRIIKHVRIKDFARLRGWRIVRIAVASELQDKGFGSELLKMIYEEAKDKG
MDWVGSSFMSDQKVLNFWIKNGFAPVHISPKKNEKLGDYPVVVIRPISDIATKIVKISVYMLKEKLLNTLHDVYFNMNPE
VVRLILKGSSIAHKTVDVNPILLDKTISFLQGVSPYESSADGIHMLTLKYFWDGERDWGLTQDEELVLIAKVIQGKPWSY
VSTILNSNRTHIYELIYSAISKIMQKYYNLTADSKVGLTLKDVMSSQQYE

Sequences:

>Translated_770_residues
MVNKEQFYDQIRKALEDGNIRYYRNLVYIEREDYLDYVKEIINLFLKFKPDPSVAYGFVPWASGSKERMKTIKEYFLKFN
DIDYANAEYYLGNTYDLVILDTVDNFQPINIGRLVDLARGGGLIIIYTNNLTKDKAFRTSILRNGLVLDEYEKRFKRKLY
EHEGIFIVDANGYVSKPFSGNIMLKSEKKVPRNPVMPREIHELSLSEDQNRVIENFTYLLSGGQRALVLTAARGRGKSAA
TGLSIAGLIEKLRERKGQSVRVIVTAPSIANASQVMSFAKLGLEVLGEELSVKVSDTGHIKTLRGDYFKVEYVPPDAAVE
DDGELLIVDEAAALGINYIDLALRAWKKVALVTTVHGYEGSNKAFLRYLRRLIESKRIRVKWINIEQPLRYANGDPIEKW
LYDALLLDAEPSEPQYLNHTMIYEDVDKSELANDDIKLRAIYGIMVTAHYKNNPDDLMIMLDGVHHKIKAIRIGENSYVA
ACQIAEEGELSDNMVDIALKGGTFEGDLIPDRIIKHVRIKDFARLRGWRIVRIAVASELQDKGFGSELLKMIYEEAKDKG
MDWVGSSFMSDQKVLNFWIKNGFAPVHISPKKNEKLGDYPVVVIRPISDIATKIVKISVYMLKEKLLNTLHDVYFNMNPE
VVRLILKGSSIAHKTVDVNPILLDKTISFLQGVSPYESSADGIHMLTLKYFWDGERDWGLTQDEELVLIAKVIQGKPWSY
VSTILNSNRTHIYELIYSAISKIMQKYYNLTADSKVGLTLKDVMSSQQYE
>Mature_770_residues
MVNKEQFYDQIRKALEDGNIRYYRNLVYIEREDYLDYVKEIINLFLKFKPDPSVAYGFVPWASGSKERMKTIKEYFLKFN
DIDYANAEYYLGNTYDLVILDTVDNFQPINIGRLVDLARGGGLIIIYTNNLTKDKAFRTSILRNGLVLDEYEKRFKRKLY
EHEGIFIVDANGYVSKPFSGNIMLKSEKKVPRNPVMPREIHELSLSEDQNRVIENFTYLLSGGQRALVLTAARGRGKSAA
TGLSIAGLIEKLRERKGQSVRVIVTAPSIANASQVMSFAKLGLEVLGEELSVKVSDTGHIKTLRGDYFKVEYVPPDAAVE
DDGELLIVDEAAALGINYIDLALRAWKKVALVTTVHGYEGSNKAFLRYLRRLIESKRIRVKWINIEQPLRYANGDPIEKW
LYDALLLDAEPSEPQYLNHTMIYEDVDKSELANDDIKLRAIYGIMVTAHYKNNPDDLMIMLDGVHHKIKAIRIGENSYVA
ACQIAEEGELSDNMVDIALKGGTFEGDLIPDRIIKHVRIKDFARLRGWRIVRIAVASELQDKGFGSELLKMIYEEAKDKG
MDWVGSSFMSDQKVLNFWIKNGFAPVHISPKKNEKLGDYPVVVIRPISDIATKIVKISVYMLKEKLLNTLHDVYFNMNPE
VVRLILKGSSIAHKTVDVNPILLDKTISFLQGVSPYESSADGIHMLTLKYFWDGERDWGLTQDEELVLIAKVIQGKPWSY
VSTILNSNRTHIYELIYSAISKIMQKYYNLTADSKVGLTLKDVMSSQQYE

Specific function: Catalyzes the formation of N(4)-acetylcytidine (ac(4)C) at the wobble position of tRNA(Met), by using acetyl-CoA as an acetyl donor and ATP (or GTP) [H]

COG id: COG1444

COG function: function code R; Predicted P-loop ATPase fused to an acetyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 N-acetyltransferase domain [H]

Homologues:

Organism=Homo sapiens, GI221316723, Length=772, Percent_Identity=23.8341968911917, Blast_Score=144, Evalue=3e-34,
Organism=Homo sapiens, GI221316741, Length=679, Percent_Identity=24.5949926362297, Blast_Score=140, Evalue=3e-33,
Organism=Escherichia coli, GI1788818, Length=405, Percent_Identity=28.1481481481481, Blast_Score=143, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI17507621, Length=550, Percent_Identity=25.2727272727273, Blast_Score=137, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6324197, Length=848, Percent_Identity=24.0566037735849, Blast_Score=175, Evalue=3e-44,
Organism=Drosophila melanogaster, GI24640662, Length=918, Percent_Identity=23.9651416122004, Blast_Score=165, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000182
- InterPro:   IPR016181
- InterPro:   IPR013562
- InterPro:   IPR007807 [H]

Pfam domain/function: PF00583 Acetyltransf_1; PF08351 DUF1726; PF05127 DUF699 [H]

EC number: NA

Molecular weight: Translated: 87750; Mature: 87750

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNKEQFYDQIRKALEDGNIRYYRNLVYIEREDYLDYVKEIINLFLKFKPDPSVAYGFVP
CCCHHHHHHHHHHHHHCCCEEEEEEEEEEEHHHHHHHHHHHHHHHEEECCCCCEEEEEEE
WASGSKERMKTIKEYFLKFNDIDYANAEYYLGNTYDLVILDTVDNFQPINIGRLVDLARG
CCCCCHHHHHHHHHHHHHCCCCCCCCCEEEECCEEEEEEEECCCCCCCCCHHHHHHHCCC
GGLIIIYTNNLTKDKAFRTSILRNGLVLDEYEKRFKRKLYEHEGIFIVDANGYVSKPFSG
CCEEEEEECCCCCHHHHHHHHHHCCCEEHHHHHHHHHHHHHCCCEEEEECCCEECCCCCC
NIMLKSEKKVPRNPVMPREIHELSLSEDQNRVIENFTYLLSGGQRALVLTAARGRGKSAA
CEEEEECCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCC
TGLSIAGLIEKLRERKGQSVRVIVTAPSIANASQVMSFAKLGLEVLGEELSVKVSDTGHI
CCHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCE
KTLRGDYFKVEYVPPDAAVEDDGELLIVDEAAALGINYIDLALRAWKKVALVTTVHGYEG
EEECCCEEEEEEECCCCCCCCCCCEEEEECHHHCCCHHHHHHHHHHHHEEEEEEEECCCC
SNKAFLRYLRRLIESKRIRVKWINIEQPLRYANGDPIEKWLYDALLLDAEPSEPQYLNHT
CCHHHHHHHHHHHHCCCEEEEEECCCCCHHCCCCCHHHHHHHHHHEECCCCCCCCCCCCE
MIYEDVDKSELANDDIKLRAIYGIMVTAHYKNNPDDLMIMLDGVHHKIKAIRIGENSYVA
EEEECCCHHHCCCCCEEEEEEEEEEEEEEECCCCCEEEEEEECCCCEEEEEEECCCCEEE
ACQIAEEGELSDNMVDIALKGGTFEGDLIPDRIIKHVRIKDFARLRGWRIVRIAVASELQ
EEEECCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHH
DKGFGSELLKMIYEEAKDKGMDWVGSSFMSDQKVLNFWIKNGFAPVHISPKKNEKLGDYP
CCCCHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHCCEEEEEECCCCCCCCCCCC
VVVIRPISDIATKIVKISVYMLKEKLLNTLHDVYFNMNPEVVRLILKGSSIAHKTVDVNP
EEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEECCCCCEEEEECCCC
ILLDKTISFLQGVSPYESSADGIHMLTLKYFWDGERDWGLTQDEELVLIAKVIQGKPWSY
EEHHHHHHHHCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCEEEEEHHHCCCCHHH
VSTILNSNRTHIYELIYSAISKIMQKYYNLTADSKVGLTLKDVMSSQQYE
HHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHCCCCCC
>Mature Secondary Structure
MVNKEQFYDQIRKALEDGNIRYYRNLVYIEREDYLDYVKEIINLFLKFKPDPSVAYGFVP
CCCHHHHHHHHHHHHHCCCEEEEEEEEEEEHHHHHHHHHHHHHHHEEECCCCCEEEEEEE
WASGSKERMKTIKEYFLKFNDIDYANAEYYLGNTYDLVILDTVDNFQPINIGRLVDLARG
CCCCCHHHHHHHHHHHHHCCCCCCCCCEEEECCEEEEEEEECCCCCCCCCHHHHHHHCCC
GGLIIIYTNNLTKDKAFRTSILRNGLVLDEYEKRFKRKLYEHEGIFIVDANGYVSKPFSG
CCEEEEEECCCCCHHHHHHHHHHCCCEEHHHHHHHHHHHHHCCCEEEEECCCEECCCCCC
NIMLKSEKKVPRNPVMPREIHELSLSEDQNRVIENFTYLLSGGQRALVLTAARGRGKSAA
CEEEEECCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCC
TGLSIAGLIEKLRERKGQSVRVIVTAPSIANASQVMSFAKLGLEVLGEELSVKVSDTGHI
CCHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCE
KTLRGDYFKVEYVPPDAAVEDDGELLIVDEAAALGINYIDLALRAWKKVALVTTVHGYEG
EEECCCEEEEEEECCCCCCCCCCCEEEEECHHHCCCHHHHHHHHHHHHEEEEEEEECCCC
SNKAFLRYLRRLIESKRIRVKWINIEQPLRYANGDPIEKWLYDALLLDAEPSEPQYLNHT
CCHHHHHHHHHHHHCCCEEEEEECCCCCHHCCCCCHHHHHHHHHHEECCCCCCCCCCCCE
MIYEDVDKSELANDDIKLRAIYGIMVTAHYKNNPDDLMIMLDGVHHKIKAIRIGENSYVA
EEEECCCHHHCCCCCEEEEEEEEEEEEEEECCCCCEEEEEEECCCCEEEEEEECCCCEEE
ACQIAEEGELSDNMVDIALKGGTFEGDLIPDRIIKHVRIKDFARLRGWRIVRIAVASELQ
EEEECCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHH
DKGFGSELLKMIYEEAKDKGMDWVGSSFMSDQKVLNFWIKNGFAPVHISPKKNEKLGDYP
CCCCHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHCCEEEEEECCCCCCCCCCCC
VVVIRPISDIATKIVKISVYMLKEKLLNTLHDVYFNMNPEVVRLILKGSSIAHKTVDVNP
EEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEECCCCCEEEEECCCC
ILLDKTISFLQGVSPYESSADGIHMLTLKYFWDGERDWGLTQDEELVLIAKVIQGKPWSY
EEHHHHHHHHCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCEEEEEHHHCCCCHHH
VSTILNSNRTHIYELIYSAISKIMQKYYNLTADSKVGLTLKDVMSSQQYE
HHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA