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The map label for this gene is nth [H]

Identifier: 227828269

GI number: 227828269

Start: 1843064

End: 1843765

Strand: Direct

Name: nth [H]

Synonym: M1425_2010

Alternate gene names: 227828269

Gene position: 1843064-1843765 (Clockwise)

Preceding gene: 227828267

Following gene: 227828270

Centisome position: 70.65

GC content: 33.62

Gene sequence:

>702_bases
GTGAAGTGTGCCCCCGAAATAGTTTATCATAAACTTTCCGCTATCTATACTATAAAAGAGGAGGATTACATTGCATATTA
TGTCTGGCTTAAGACTAGAAATTGTTTTAAAGTACTTGTAGCCACTATCTTATCTCAAAATTCTACAGATAAATCAGCGC
TTAAAGCCTATTTAGAGCTAGAAAAGAAAGTTGGCGTTACTCCAGAAAAACTATCAGATGCGGATTTGAGTGATATAGAA
AATGCGCTAAAAATATCTGGCCTTTACAAAACTAAGGCCAAAAGGCTAAAAATAATTTCAAAAATAATTCTGGAGAAATA
TAATGGATTGATAGATAATCTGCTAAATAGTAGTAATCCCAGAGAGGAACTATTAAAATTCGAGGGAATAGGAGAGAAAA
CTGCAGATGTTGTATTATTAACTTGTAGGGGATACGAAGTTTTCCCGATTGACACTCACATAACTAGAGTTAGTAAAAGG
TTAGGGATAGTTCCTATGAACGCTAAATATGAATTGATATCATCTACTTTAAAAGAACTTTTCTCGGCTTATGATCTTCT
TCAACTTCATCACTTATTAATTGCACATGGAAGACAAACTTGCAAGGCTAGAAAACCTTTATGTAATTCATGTATAATTA
AAGAATGTTGTGAGTACTATTCGCATAGAGATGGAGAAGCCCGGAAATCTAATACCTCATGA

Upstream 100 bases:

>100_bases
AGCACCTTCCTTTATGGCATCAAGAACCATCTCTATTTCGTACATCAAATAATAAGTTAGCTTAAGAATTCCTTATAAGC
TGAACTGCATATTCAAATTC

Downstream 100 bases:

>100_bases
GGACATATTAATCGACAAAGTTGTCAATATCTCTTTAGAAGTGCAGAAAATAAGGGCTATAAAACCTATTATAGTGGATG
AAAATACACATGTTATCTTA

Product: DNA-(apurinic or apyrimidinic site) lyase

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 233; Mature: 233

Protein sequence:

>233_residues
MKCAPEIVYHKLSAIYTIKEEDYIAYYVWLKTRNCFKVLVATILSQNSTDKSALKAYLELEKKVGVTPEKLSDADLSDIE
NALKISGLYKTKAKRLKIISKIILEKYNGLIDNLLNSSNPREELLKFEGIGEKTADVVLLTCRGYEVFPIDTHITRVSKR
LGIVPMNAKYELISSTLKELFSAYDLLQLHHLLIAHGRQTCKARKPLCNSCIIKECCEYYSHRDGEARKSNTS

Sequences:

>Translated_233_residues
MKCAPEIVYHKLSAIYTIKEEDYIAYYVWLKTRNCFKVLVATILSQNSTDKSALKAYLELEKKVGVTPEKLSDADLSDIE
NALKISGLYKTKAKRLKIISKIILEKYNGLIDNLLNSSNPREELLKFEGIGEKTADVVLLTCRGYEVFPIDTHITRVSKR
LGIVPMNAKYELISSTLKELFSAYDLLQLHHLLIAHGRQTCKARKPLCNSCIIKECCEYYSHRDGEARKSNTS
>Mature_233_residues
MKCAPEIVYHKLSAIYTIKEEDYIAYYVWLKTRNCFKVLVATILSQNSTDKSALKAYLELEKKVGVTPEKLSDADLSDIE
NALKISGLYKTKAKRLKIISKIILEKYNGLIDNLLNSSNPREELLKFEGIGEKTADVVLLTCRGYEVFPIDTHITRVSKR
LGIVPMNAKYELISSTLKELFSAYDLLQLHHLLIAHGRQTCKARKPLCNSCIIKECCEYYSHRDGEARKSNTS

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Escherichia coli, GI1787920, Length=189, Percent_Identity=37.037037037037, Blast_Score=115, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17554540, Length=199, Percent_Identity=28.643216080402, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI45550361, Length=199, Percent_Identity=29.6482412060301, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 26512; Mature: 26512

Theoretical pI: Translated: 8.98; Mature: 8.98

Prosite motif: PS00764 ENDONUCLEASE_III_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKCAPEIVYHKLSAIYTIKEEDYIAYYVWLKTRNCFKVLVATILSQNSTDKSALKAYLEL
CCCCHHHHHHHHHHHEEECCCCEEEEEEEEECCHHHHHHHHHHHCCCCCCHHHHHHHHHH
EKKVGVTPEKLSDADLSDIENALKISGLYKTKAKRLKIISKIILEKYNGLIDNLLNSSNP
HHHCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
REELLKFEGIGEKTADVVLLTCRGYEVFPIDTHITRVSKRLGIVPMNAKYELISSTLKEL
HHHHHHHCCCCCCCCCEEEEEECCEEEEECHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
FSAYDLLQLHHLLIAHGRQTCKARKPLCNSCIIKECCEYYSHRDGEARKSNTS
HHHHHHHHHHHHHHHHCHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MKCAPEIVYHKLSAIYTIKEEDYIAYYVWLKTRNCFKVLVATILSQNSTDKSALKAYLEL
CCCCHHHHHHHHHHHEEECCCCEEEEEEEEECCHHHHHHHHHHHCCCCCCHHHHHHHHHH
EKKVGVTPEKLSDADLSDIENALKISGLYKTKAKRLKIISKIILEKYNGLIDNLLNSSNP
HHHCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
REELLKFEGIGEKTADVVLLTCRGYEVFPIDTHITRVSKRLGIVPMNAKYELISSTLKEL
HHHHHHHCCCCCCCCCEEEEEECCEEEEECHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
FSAYDLLQLHHLLIAHGRQTCKARKPLCNSCIIKECCEYYSHRDGEARKSNTS
HHHHHHHHHHHHHHHHCHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]