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The map label for this gene is rsmB [H]

Identifier: 227828262

GI number: 227828262

Start: 1835672

End: 1836778

Strand: Direct

Name: rsmB [H]

Synonym: M1425_2003

Alternate gene names: 227828262

Gene position: 1835672-1836778 (Clockwise)

Preceding gene: 227828261

Following gene: 227828263

Centisome position: 70.36

GC content: 32.25

Gene sequence:

>1107_bases
TTGAAGGATATAGTAAAGTTTTTGACTACTGTGCTATATTACGTGGAAAAGAAGAATTATCCCTTGGCGGTAGCGTTTAA
ACGAGCGTATCTGCACAACAAACCAAGGAAGATAGAGTCTAACCTCTTATACGAATATAGTAAAAGGATGCTATTATCTT
ATTATGCATTACCTCAAAGCAAAAGGTCATTTAAGGTAAGATATTGGCTTGAAAATAAGGAAAGTATTGAGATTAAGTTT
CCTAACTGGATGGAAGAAAAACTATCCACATTATTAGATATTAATGCTCTAAAAAGAAAACTATCAGAAAGATGGATGTG
GGCTCGTGTAAATATCCTCAAGACTGATATCGATAAAATTTATAGGGAGTTAGAGAGTCAAAATATAGAATTTGAAGTGG
ATAAGAATTTTTATTATATGATAAAGATCAAGAAGTCACCAGTAAGATTATCTTCTTTGAGCATAGTAAAAGACTGTAAA
TTAGTAATTCACGATAAAGCTAGTTCCTTAGTGGTAGAAGCACTTAAGCCAGAGATAGGTGAAAAACTAGTCGATCTGTC
TTCAGCTCCCGGGATTAAGGCTTCTTTATACATGATGCTAACAGAAAATAGAGCGGAAACTTTCCTAGCCGACGTCGATT
TTAAGAGGTTATCGAGGGAATATAATTTGTTAAAAAGGTGTGGAGTAGATTTACGTAAGATTCATATAATATATCAAGAT
TCCACAAAAAATAGCGTAATAAAATCAGATAAGATATTATTAGATGCGCCATGTTCCTCCTCCGGAATGATAAATAATGA
TCCTTCTATTTTATTGAAATTAAGTGATAATGAAAAAATAAAAAAGTTCGCTAAATTGCAGAAATCTTTACTAAATGAGT
CCTTAAAGATTAGGGCCAATAAACTCGTTTATGCAGTATGTTCATTATTTCCAGAAGAGGGTGAAAGGGTAATAGAAGAC
TATTATGATATAGCTGAAATGCCATTTTCTAATTATCAATCTGGTTATTCTTTATACAAGGTAGGAAAGAGATCCAATAG
GACTTTCCCTCATATCGATTCTACTGAAGGGTTCTTTATATCAGTCTTGAATTTAACTAAATTATAA

Upstream 100 bases:

>100_bases
GTGTAATCTAATATAGAGTAATCTTATGTAAGTCCTAATATTACCTTATGCCTAGATGACGATAAGAATATTAAACGTTA
AAATTTGAATTACACTTAAG

Downstream 100 bases:

>100_bases
ATTCACATTACACTCCGTCTTTTTATAAAGGATTAAAAAGAGGATATATGGTGATTCCTGATTTTATACTTTACCAGAAA
TTAGATTTAAATTTTATCAC

Product: Fmu (Sun) domain protein

Products: NA

Alternate protein names: 16S rRNA m5C967 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MKDIVKFLTTVLYYVEKKNYPLAVAFKRAYLHNKPRKIESNLLYEYSKRMLLSYYALPQSKRSFKVRYWLENKESIEIKF
PNWMEEKLSTLLDINALKRKLSERWMWARVNILKTDIDKIYRELESQNIEFEVDKNFYYMIKIKKSPVRLSSLSIVKDCK
LVIHDKASSLVVEALKPEIGEKLVDLSSAPGIKASLYMMLTENRAETFLADVDFKRLSREYNLLKRCGVDLRKIHIIYQD
STKNSVIKSDKILLDAPCSSSGMINNDPSILLKLSDNEKIKKFAKLQKSLLNESLKIRANKLVYAVCSLFPEEGERVIED
YYDIAEMPFSNYQSGYSLYKVGKRSNRTFPHIDSTEGFFISVLNLTKL

Sequences:

>Translated_368_residues
MKDIVKFLTTVLYYVEKKNYPLAVAFKRAYLHNKPRKIESNLLYEYSKRMLLSYYALPQSKRSFKVRYWLENKESIEIKF
PNWMEEKLSTLLDINALKRKLSERWMWARVNILKTDIDKIYRELESQNIEFEVDKNFYYMIKIKKSPVRLSSLSIVKDCK
LVIHDKASSLVVEALKPEIGEKLVDLSSAPGIKASLYMMLTENRAETFLADVDFKRLSREYNLLKRCGVDLRKIHIIYQD
STKNSVIKSDKILLDAPCSSSGMINNDPSILLKLSDNEKIKKFAKLQKSLLNESLKIRANKLVYAVCSLFPEEGERVIED
YYDIAEMPFSNYQSGYSLYKVGKRSNRTFPHIDSTEGFFISVLNLTKL
>Mature_368_residues
MKDIVKFLTTVLYYVEKKNYPLAVAFKRAYLHNKPRKIESNLLYEYSKRMLLSYYALPQSKRSFKVRYWLENKESIEIKF
PNWMEEKLSTLLDINALKRKLSERWMWARVNILKTDIDKIYRELESQNIEFEVDKNFYYMIKIKKSPVRLSSLSIVKDCK
LVIHDKASSLVVEALKPEIGEKLVDLSSAPGIKASLYMMLTENRAETFLADVDFKRLSREYNLLKRCGVDLRKIHIIYQD
STKNSVIKSDKILLDAPCSSSGMINNDPSILLKLSDNEKIKKFAKLQKSLLNESLKIRANKLVYAVCSLFPEEGERVIED
YYDIAEMPFSNYQSGYSLYKVGKRSNRTFPHIDSTEGFFISVLNLTKL

Specific function: Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI8922322, Length=304, Percent_Identity=25.6578947368421, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI23199998, Length=308, Percent_Identity=26.2987012987013, Blast_Score=86, Evalue=8e-17,
Organism=Homo sapiens, GI270288818, Length=308, Percent_Identity=25.974025974026, Blast_Score=85, Evalue=9e-17,
Organism=Homo sapiens, GI270288816, Length=304, Percent_Identity=25.6578947368421, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI76150625, Length=222, Percent_Identity=31.5315315315315, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI76150623, Length=222, Percent_Identity=31.5315315315315, Blast_Score=82, Evalue=6e-16,
Organism=Escherichia coli, GI2367212, Length=268, Percent_Identity=27.6119402985075, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI87081985, Length=217, Percent_Identity=27.1889400921659, Blast_Score=72, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI115534021, Length=227, Percent_Identity=28.1938325991189, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71998419, Length=227, Percent_Identity=28.1938325991189, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17536757, Length=212, Percent_Identity=28.7735849056604, Blast_Score=81, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324268, Length=280, Percent_Identity=26.0714285714286, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI22024126, Length=225, Percent_Identity=27.5555555555556, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI21356579, Length=165, Percent_Identity=26.0606060606061, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: =2.1.1.176 [H]

Molecular weight: Translated: 43027; Mature: 43027

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDIVKFLTTVLYYVEKKNYPLAVAFKRAYLHNKPRKIESNLLYEYSKRMLLSYYALPQS
CHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
KRSFKVRYWLENKESIEIKFPNWMEEKLSTLLDINALKRKLSERWMWARVNILKTDIDKI
CCCEEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YRELESQNIEFEVDKNFYYMIKIKKSPVRLSSLSIVKDCKLVIHDKASSLVVEALKPEIG
HHHHHCCCCEEEECCCEEEEEEEECCCCEEHHHHHHHHCEEEEECCHHHHHHHHHCHHHH
EKLVDLSSAPGIKASLYMMLTENRAETFLADVDFKRLSREYNLLKRCGVDLRKIHIIYQD
HHHHCCCCCCCCCEEEEEEEECCCHHHHEECCCHHHHHHHHHHHHHCCCCEEEEEEEEEC
STKNSVIKSDKILLDAPCSSSGMINNDPSILLKLSDNEKIKKFAKLQKSLLNESLKIRAN
CCCCCEEECCCEEEECCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCHHHHHHH
KLVYAVCSLFPEEGERVIEDYYDIAEMPFSNYQSGYSLYKVGKRSNRTFPHIDSTEGFFI
HHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCHHEEECCCCCCCCCCCCCCCCCEE
SVLNLTKL
EEEEHHCC
>Mature Secondary Structure
MKDIVKFLTTVLYYVEKKNYPLAVAFKRAYLHNKPRKIESNLLYEYSKRMLLSYYALPQS
CHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
KRSFKVRYWLENKESIEIKFPNWMEEKLSTLLDINALKRKLSERWMWARVNILKTDIDKI
CCCEEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YRELESQNIEFEVDKNFYYMIKIKKSPVRLSSLSIVKDCKLVIHDKASSLVVEALKPEIG
HHHHHCCCCEEEECCCEEEEEEEECCCCEEHHHHHHHHCEEEEECCHHHHHHHHHCHHHH
EKLVDLSSAPGIKASLYMMLTENRAETFLADVDFKRLSREYNLLKRCGVDLRKIHIIYQD
HHHHCCCCCCCCCEEEEEEEECCCHHHHEECCCHHHHHHHHHHHHHCCCCEEEEEEEEEC
STKNSVIKSDKILLDAPCSSSGMINNDPSILLKLSDNEKIKKFAKLQKSLLNESLKIRAN
CCCCCEEECCCEEEECCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCHHHHHHH
KLVYAVCSLFPEEGERVIEDYYDIAEMPFSNYQSGYSLYKVGKRSNRTFPHIDSTEGFFI
HHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCHHEEECCCCCCCCCCCCCCCCCEE
SVLNLTKL
EEEEHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA