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The map label for this gene is btuF [H]

Identifier: 227828256

GI number: 227828256

Start: 1829765

End: 1830580

Strand: Direct

Name: btuF [H]

Synonym: M1425_1997

Alternate gene names: 227828256

Gene position: 1829765-1830580 (Clockwise)

Preceding gene: 227828255

Following gene: 227828258

Centisome position: 70.14

GC content: 33.82

Gene sequence:

>816_bases
ATGAGTATAAGAGTTTATAATCCATTACAAGATGATTATATAAGAATTCCGAAGCCAATTACTAAAATAGTTAGTCTAGA
TCCAGCTTCTACAGAGATAATATTTCTTTTAGGCTTAGGAGATAAGGTTAAAGCCACAGACGCCTTTAGTTATAGACCAG
AAGAGGCGAGAAGAACATTAAAGGTAGGTAGTTATACTCACGTTAATATGGATATCTTAGAGCAAATTAATCCGGATATA
ATATTTACTACTGCAGGAGCACAAAAGGAGCTAACAAGAAAATTAATTGGTTTAGGTTTTAACGTATATCCCTTACCAGT
TCCAACTAACATCGCAGGGATTTTGAATAACGTTTTACTTATTGGAAATGTTTTAGGAGCCTACCAAAACGCCAGAGAAT
TATATTATAATCTTTACACTATTATAAATGAGCTTAAAGTTGGGAGGTTTAAGAATAAGCTAAAGGTCTATGTGGAACTG
GACCTAGGCGGACCTATAACAGTAGGTTTTCCAACTCATATCAGTGACGGAATATCACTATTAGGAAGTATTAATATTTT
CGATGACGTTTCTGAAGCCTATTTCACACCCAATGATGACGAAATCTTGAAAAGAAATCCAGACATTCTAATTTACGAGC
CGAAAAAGCTTACAGATTACGAAAAGGAGAGATTTTATTCAATTTTACAACAAAGAGGATTATTTCAGCTGTTAAGTAAG
CGTATAATCTTCACCAAGGGTGATTATTTAGCTCATATGGGACCTAGTTTCATAACTGATGCACTGATTTGGCTTAAATC
AGTTGTTTTTTCTTAA

Upstream 100 bases:

>100_bases
CAAGTTAAGGTTTTGTTAAGGGATGTGAGAAGAGCGTTTGGAGTTATAACTTGCCTTGGAGTTAAATAAATATAAAGATG
TATTCTGTTAATTTTAATAA

Downstream 100 bases:

>100_bases
CCTTAGCTTCCTAGTTATCCTACTTCCTTTAGCTAATCCTCGGATTATTGAATACAAACCCAATATTATGCTTCCTGCCA
TGATCACTTCATAAATCACT

Product: periplasmic binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MSIRVYNPLQDDYIRIPKPITKIVSLDPASTEIIFLLGLGDKVKATDAFSYRPEEARRTLKVGSYTHVNMDILEQINPDI
IFTTAGAQKELTRKLIGLGFNVYPLPVPTNIAGILNNVLLIGNVLGAYQNARELYYNLYTIINELKVGRFKNKLKVYVEL
DLGGPITVGFPTHISDGISLLGSINIFDDVSEAYFTPNDDEILKRNPDILIYEPKKLTDYEKERFYSILQQRGLFQLLSK
RIIFTKGDYLAHMGPSFITDALIWLKSVVFS

Sequences:

>Translated_271_residues
MSIRVYNPLQDDYIRIPKPITKIVSLDPASTEIIFLLGLGDKVKATDAFSYRPEEARRTLKVGSYTHVNMDILEQINPDI
IFTTAGAQKELTRKLIGLGFNVYPLPVPTNIAGILNNVLLIGNVLGAYQNARELYYNLYTIINELKVGRFKNKLKVYVEL
DLGGPITVGFPTHISDGISLLGSINIFDDVSEAYFTPNDDEILKRNPDILIYEPKKLTDYEKERFYSILQQRGLFQLLSK
RIIFTKGDYLAHMGPSFITDALIWLKSVVFS
>Mature_270_residues
SIRVYNPLQDDYIRIPKPITKIVSLDPASTEIIFLLGLGDKVKATDAFSYRPEEARRTLKVGSYTHVNMDILEQINPDII
FTTAGAQKELTRKLIGLGFNVYPLPVPTNIAGILNNVLLIGNVLGAYQNARELYYNLYTIINELKVGRFKNKLKVYVELD
LGGPITVGFPTHISDGISLLGSINIFDDVSEAYFTPNDDEILKRNPDILIYEPKKLTDYEKERFYSILQQRGLFQLLSKR
IIFTKGDYLAHMGPSFITDALIWLKSVVFS

Specific function: Part of the ABC transporter complex BtuCDF involved in vitamin B12 import. Binds vitamin B12 and delivers it to the periplasmic surface of BtuC [H]

COG id: COG0614

COG function: function code P; ABC-type Fe3+-hydroxamate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe/B12 periplasmic-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002491 [H]

Pfam domain/function: PF01497 Peripla_BP_2 [H]

EC number: NA

Molecular weight: Translated: 30684; Mature: 30553

Theoretical pI: Translated: 7.70; Mature: 7.70

Prosite motif: PS50983 FE_B12_PBP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIRVYNPLQDDYIRIPKPITKIVSLDPASTEIIFLLGLGDKVKATDAFSYRPEEARRTL
CEEEECCCCCCCCEECCCHHHHHEECCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHE
KVGSYTHVNMDILEQINPDIIFTTAGAQKELTRKLIGLGFNVYPLPVPTNIAGILNNVLL
ECCCEEEECHHHHHHCCCCEEEEECCCHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHH
IGNVLGAYQNARELYYNLYTIINELKVGRFKNKLKVYVELDLGGPITVGFPTHISDGISL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCEEEECCCCHHHHHHHH
LGSINIFDDVSEAYFTPNDDEILKRNPDILIYEPKKLTDYEKERFYSILQQRGLFQLLSK
HCCCCHHHCCHHHCCCCCCCHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHCCHHHHHHC
RIIFTKGDYLAHMGPSFITDALIWLKSVVFS
CEEEECCCHHHHCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SIRVYNPLQDDYIRIPKPITKIVSLDPASTEIIFLLGLGDKVKATDAFSYRPEEARRTL
EEEECCCCCCCCEECCCHHHHHEECCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHE
KVGSYTHVNMDILEQINPDIIFTTAGAQKELTRKLIGLGFNVYPLPVPTNIAGILNNVLL
ECCCEEEECHHHHHHCCCCEEEEECCCHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHH
IGNVLGAYQNARELYYNLYTIINELKVGRFKNKLKVYVELDLGGPITVGFPTHISDGISL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCEEEECCCCHHHHHHHH
LGSINIFDDVSEAYFTPNDDEILKRNPDILIYEPKKLTDYEKERFYSILQQRGLFQLLSK
HCCCCHHHCCHHHCCCCCCCHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHCCHHHHHHC
RIIFTKGDYLAHMGPSFITDALIWLKSVVFS
CEEEECCCHHHHCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA