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The map label for this gene is pyrG [H]

Identifier: 227828194

GI number: 227828194

Start: 1772420

End: 1774027

Strand: Direct

Name: pyrG [H]

Synonym: M1425_1932

Alternate gene names: 227828194

Gene position: 1772420-1774027 (Clockwise)

Preceding gene: 227828191

Following gene: 227828196

Centisome position: 67.94

GC content: 34.45

Gene sequence:

>1608_bases
GTGTCCAACAAGTACATAATCGTTACAGGTGGAGTTTTATCAAGTGTTGGTAAAGGTACGTTAGTTGCCTCTATTGGTAT
GCTACTTAAAAGAAGAGGATATAATGTAACTGCAGTAAAAGTAGATCCATATATTAATGTTGATGCGGGTACAATGAATC
CTTATATGCATGGTGAAGTATTTGTAACTGAGGACGGTGCAGAGACAGATTTAGATTTAGGTCATTATGAGAGATTTATG
GATATTAATATGACAAAGTATAATAATATAACAGCAGGTAAGGTATATTTTGAGGTTATAAAGAAGGAAAGAGAAGGCAA
ATATCTAGGTCAAACCGTGCAAATTATCCCCCACGTTACTGATCAGATAAAGGATATGATAAGATATGCAAGTAAGATAA
ATAACGCTGAGATTACCTTAGTGGAAATAGGTGGCACTGTAGGTGACATAGAAAGTCTACCATTTCTAGAAGCAGCTAGA
CAACTCAAACTAGAAGAAGGCGAAGATAATGTTGTTTTTGTGCATATAGCTTTAGTAGAATATTTATCTGTAACTGGAGA
ACTGAAAACTAAACCATTGCAGCATAGTGTGCAAGAATTAAGAAGAATTGGAATACAACCAGATTTTATTGTTGGCAGAG
CCACATTAGCCTTAGATGATGAAACTAGGCGCAAAATTGCTCTGTTCACTAACGTTAAGGTAGATCATATAGTCTCTAGC
TATGATGTAGAAACATCCTACGAAGTACCCATTATCCTAGAAAACCAGAAATTAGTTTCTAAGATACTTACTAGGCTTAA
ATTAGAGGATAGGCAAGTAGATTTGACAGATTGGATAAGCTTCGTTAATAACATAAAAGGAATAAATAGTAAAAAGACCA
TTAATATTGCACTAGTTGGAAAGTACACAAAGCTAAAGGATAGTTACATAAGTATCAAGGAAGCGATTTATCACGCTTCA
GCTTATTTAGGAGTAAGGCCTAATCTTATATGGATAGAATCAACTGATTTGGAAAAGGATTCCAAGAATTTAGATGAGGT
ATTAGGGAATGTAAATGGAATCATAGTTTTGCCTGGATTTGGAAGTAGAGGAGCTGAAGGTAAGATTAAGGCTATTAAAT
ATGCTAGAGAACATAATGTACCGTTCTTGGGTATATGCTTTGGTTTTCAATTATCTATTGTAGAATTCGCTAGGAATGTT
TTAGGTCTCAATGATGCTAATTCGACTGAAATTAATCCAAATACTAAGGATCCAGTTATTACTTTACTGGATGAGCAAAA
AAATGTAACTCAACTTGGGGGGACTATGAGATTAGGATCACAAAAGATTATAATAAAAGAAGGAACAATTTCCTATCAAT
TATATGGTAAAAAGGTTGTATATGAAAGGCATAGACATAGATATGAAGTGAATCCTAAATATGTCGATTTACTAGAAGAT
GCAGGATTAGTAGTTTCTGGTATTAGTGAAAACGGCTTGGTCGAAATTATAGAGCTACCTTCTAATAAGTTCTTTGTGGC
CACTCAAGCTCATCCAGAATTCAAGAGCAGACCAACAAATCCATCTCCTATATATTTAGGGTTCATTAGGGCTGTTGCTA
GTCTTTAA

Upstream 100 bases:

>100_bases
CCTCTCTCATTTAAGGTAAATATGTGCTTTAAAAGTACTTAAAGTAATTGACAGTGACTTTTTATTTTATAGCAGTGAAT
GCAAATTACTGTGGATATTA

Downstream 100 bases:

>100_bases
AACTATTACAATTTTATCATATTTGGCTTTTATAATCTTTTTTATTCCTTTTATTCCATTCTGATACTCGACTTCAACCA
GACCTAGATTTTCAAGTTCA

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase [H]

Number of amino acids: Translated: 535; Mature: 534

Protein sequence:

>535_residues
MSNKYIIVTGGVLSSVGKGTLVASIGMLLKRRGYNVTAVKVDPYINVDAGTMNPYMHGEVFVTEDGAETDLDLGHYERFM
DINMTKYNNITAGKVYFEVIKKEREGKYLGQTVQIIPHVTDQIKDMIRYASKINNAEITLVEIGGTVGDIESLPFLEAAR
QLKLEEGEDNVVFVHIALVEYLSVTGELKTKPLQHSVQELRRIGIQPDFIVGRATLALDDETRRKIALFTNVKVDHIVSS
YDVETSYEVPIILENQKLVSKILTRLKLEDRQVDLTDWISFVNNIKGINSKKTINIALVGKYTKLKDSYISIKEAIYHAS
AYLGVRPNLIWIESTDLEKDSKNLDEVLGNVNGIIVLPGFGSRGAEGKIKAIKYAREHNVPFLGICFGFQLSIVEFARNV
LGLNDANSTEINPNTKDPVITLLDEQKNVTQLGGTMRLGSQKIIIKEGTISYQLYGKKVVYERHRHRYEVNPKYVDLLED
AGLVVSGISENGLVEIIELPSNKFFVATQAHPEFKSRPTNPSPIYLGFIRAVASL

Sequences:

>Translated_535_residues
MSNKYIIVTGGVLSSVGKGTLVASIGMLLKRRGYNVTAVKVDPYINVDAGTMNPYMHGEVFVTEDGAETDLDLGHYERFM
DINMTKYNNITAGKVYFEVIKKEREGKYLGQTVQIIPHVTDQIKDMIRYASKINNAEITLVEIGGTVGDIESLPFLEAAR
QLKLEEGEDNVVFVHIALVEYLSVTGELKTKPLQHSVQELRRIGIQPDFIVGRATLALDDETRRKIALFTNVKVDHIVSS
YDVETSYEVPIILENQKLVSKILTRLKLEDRQVDLTDWISFVNNIKGINSKKTINIALVGKYTKLKDSYISIKEAIYHAS
AYLGVRPNLIWIESTDLEKDSKNLDEVLGNVNGIIVLPGFGSRGAEGKIKAIKYAREHNVPFLGICFGFQLSIVEFARNV
LGLNDANSTEINPNTKDPVITLLDEQKNVTQLGGTMRLGSQKIIIKEGTISYQLYGKKVVYERHRHRYEVNPKYVDLLED
AGLVVSGISENGLVEIIELPSNKFFVATQAHPEFKSRPTNPSPIYLGFIRAVASL
>Mature_534_residues
SNKYIIVTGGVLSSVGKGTLVASIGMLLKRRGYNVTAVKVDPYINVDAGTMNPYMHGEVFVTEDGAETDLDLGHYERFMD
INMTKYNNITAGKVYFEVIKKEREGKYLGQTVQIIPHVTDQIKDMIRYASKINNAEITLVEIGGTVGDIESLPFLEAARQ
LKLEEGEDNVVFVHIALVEYLSVTGELKTKPLQHSVQELRRIGIQPDFIVGRATLALDDETRRKIALFTNVKVDHIVSSY
DVETSYEVPIILENQKLVSKILTRLKLEDRQVDLTDWISFVNNIKGINSKKTINIALVGKYTKLKDSYISIKEAIYHASA
YLGVRPNLIWIESTDLEKDSKNLDEVLGNVNGIIVLPGFGSRGAEGKIKAIKYAREHNVPFLGICFGFQLSIVEFARNVL
GLNDANSTEINPNTKDPVITLLDEQKNVTQLGGTMRLGSQKIIIKEGTISYQLYGKKVVYERHRHRYEVNPKYVDLLEDA
GLVVSGISENGLVEIIELPSNKFFVATQAHPEFKSRPTNPSPIYLGFIRAVASL

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen [H]

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI28559085, Length=549, Percent_Identity=46.0837887067395, Blast_Score=495, Evalue=1e-140,
Organism=Homo sapiens, GI28559083, Length=549, Percent_Identity=46.0837887067395, Blast_Score=495, Evalue=1e-140,
Organism=Homo sapiens, GI221316689, Length=549, Percent_Identity=46.0837887067395, Blast_Score=495, Evalue=1e-140,
Organism=Homo sapiens, GI148491070, Length=548, Percent_Identity=46.1678832116788, Blast_Score=494, Evalue=1e-139,
Organism=Escherichia coli, GI1789142, Length=546, Percent_Identity=47.2527472527472, Blast_Score=500, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI25148299, Length=602, Percent_Identity=37.5415282392027, Blast_Score=424, Evalue=1e-119,
Organism=Saccharomyces cerevisiae, GI6319432, Length=563, Percent_Identity=43.3392539964476, Blast_Score=473, Evalue=1e-134,
Organism=Saccharomyces cerevisiae, GI6322563, Length=564, Percent_Identity=43.2624113475177, Blast_Score=467, Evalue=1e-132,
Organism=Drosophila melanogaster, GI24664469, Length=548, Percent_Identity=46.1678832116788, Blast_Score=498, Evalue=1e-141,
Organism=Drosophila melanogaster, GI21357815, Length=493, Percent_Identity=44.2190669371197, Blast_Score=422, Evalue=1e-118,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase [H]

EC number: =6.3.4.2 [H]

Molecular weight: Translated: 59768; Mature: 59636

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNKYIIVTGGVLSSVGKGTLVASIGMLLKRRGYNVTAVKVDPYINVDAGTMNPYMHGEV
CCCCEEEEECHHHHHCCCCHHHHHHHHHHHHCCCEEEEEEECCEEEECCCCCCCCCCCEE
FVTEDGAETDLDLGHYERFMDINMTKYNNITAGKVYFEVIKKEREGKYLGQTVQIIPHVT
EEECCCCCCCCCHHHHHHHEEEEEEECCCEEHHHHHHHHHHHHHCCCHHCCHHEEHHHHH
DQIKDMIRYASKINNAEITLVEIGGTVGDIESLPFLEAARQLKLEEGEDNVVFVHIALVE
HHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHHHHCCCCCCCCCEEEEEEHHHH
YLSVTGELKTKPLQHSVQELRRIGIQPDFIVGRATLALDDETRRKIALFTNVKVDHIVSS
HHHHCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCCEEEEEECCCHHHHHCC
YDVETSYEVPIILENQKLVSKILTRLKLEDRQVDLTDWISFVNNIKGINSKKTINIALVG
CCCCCCCCCEEEECCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEEEE
KYTKLKDSYISIKEAIYHASAYLGVRPNLIWIESTDLEKDSKNLDEVLGNVNGIIVLPGF
CCHHHCHHHHHHHHHHHHHHHEECCCCCEEEEECCCCCCCHHHHHHHHCCCCCEEEECCC
GSRGAEGKIKAIKYAREHNVPFLGICFGFQLSIVEFARNVLGLNDANSTEINPNTKDPVI
CCCCCCCCEEEEEEHHHCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEE
TLLDEQKNVTQLGGTMRLGSQKIIIKEGTISYQLYGKKVVYERHRHRYEVNPKYVDLLED
EEECCCCCHHHCCCEEEECCEEEEEEECEEEEEEECCHHHHHHHCCCEECCCHHHHHHHC
AGLVVSGISENGLVEIIELPSNKFFVATQAHPEFKSRPTNPSPIYLGFIRAVASL
CCEEEECCCCCCCEEEEECCCCEEEEEEECCCCHHCCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
SNKYIIVTGGVLSSVGKGTLVASIGMLLKRRGYNVTAVKVDPYINVDAGTMNPYMHGEV
CCCEEEEECHHHHHCCCCHHHHHHHHHHHHCCCEEEEEEECCEEEECCCCCCCCCCCEE
FVTEDGAETDLDLGHYERFMDINMTKYNNITAGKVYFEVIKKEREGKYLGQTVQIIPHVT
EEECCCCCCCCCHHHHHHHEEEEEEECCCEEHHHHHHHHHHHHHCCCHHCCHHEEHHHHH
DQIKDMIRYASKINNAEITLVEIGGTVGDIESLPFLEAARQLKLEEGEDNVVFVHIALVE
HHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHHHHCCCCCCCCCEEEEEEHHHH
YLSVTGELKTKPLQHSVQELRRIGIQPDFIVGRATLALDDETRRKIALFTNVKVDHIVSS
HHHHCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCCEEEEEECCCHHHHHCC
YDVETSYEVPIILENQKLVSKILTRLKLEDRQVDLTDWISFVNNIKGINSKKTINIALVG
CCCCCCCCCEEEECCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEEEE
KYTKLKDSYISIKEAIYHASAYLGVRPNLIWIESTDLEKDSKNLDEVLGNVNGIIVLPGF
CCHHHCHHHHHHHHHHHHHHHEECCCCCEEEEECCCCCCCHHHHHHHHCCCCCEEEECCC
GSRGAEGKIKAIKYAREHNVPFLGICFGFQLSIVEFARNVLGLNDANSTEINPNTKDPVI
CCCCCCCCEEEEEEHHHCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEE
TLLDEQKNVTQLGGTMRLGSQKIIIKEGTISYQLYGKKVVYERHRHRYEVNPKYVDLLED
EEECCCCCHHHCCCEEEECCEEEEEEECEEEEEEECCHHHHHHHCCCEECCCHHHHHHHC
AGLVVSGISENGLVEIIELPSNKFFVATQAHPEFKSRPTNPSPIYLGFIRAVASL
CCEEEECCCCCCCEEEEECCCCEEEEEEECCCCHHCCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11427726 [H]