Click here to switch to the map view.

The map label for this gene is purB [H]

Identifier: 227828161

GI number: 227828161

Start: 1739473

End: 1740879

Strand: Direct

Name: purB [H]

Synonym: M1425_1899

Alternate gene names: 227828161

Gene position: 1739473-1740879 (Clockwise)

Preceding gene: 227828155

Following gene: 227828162

Centisome position: 66.68

GC content: 34.4

Gene sequence:

>1407_bases
GTGAGATTACAGTATAAGTTAATAAATGTTGAACATACTTTACAATTAGCCATGGAAGATGGTATATGTCCTTTAGAATG
GAGATACGGTAGTAATGAGATGAGAAAGCTTTTCAGTAGAGGAGAAATCTTGAATAGGAGAATTCAAGTAGAAATAGCGT
TACTAAAAGCATTAAGCAAATATGGTTTAGTAAAAGAGGAAGATATCCAAAAAGTTGAGAAAGCTTCATTAACAATAAGA
CCAGAAGAAGTTGATGAACTTGAATCAAAAATCGGTCATGACGTAATGGCTATGGTAGTGCTAATGGCAGAAAGGAGTGG
AGACTCAGGGAGATATGTCCATTTTGGAGCAACAAGTTATGATATTGTAGACACAGCTTACGCGTTAATATTTAGAGACG
CAATAAGATTGGTAAAGAGAAAAATAATGGAAATATTGGAAATAATGATTAGTTTAGCAATTAAGTACAAAGATTTAGTA
ATGGTAGGGAGAACTCATGGACAACACGCATTACCTATAACCTTAGGCTTTAAAATTGCTAATTACATCTATGAATTTTC
TAGGTCGTTAGAGAGATTAAATGAGGTTGAGAAAAGAATAGTAAAAGGTAAAATAGCAGGTGCAGTTGGCACGATGGCTG
CTTGGGGCAATATAGGGTTAGAAATCGAAGAAAATATTATGAAACTTTTAGAATTAAGACCTCATGAGATTTCAACTCAA
GTTGCTCCACGAGACGCGTTTGCAGAATTAACTTCTGATCTTGCAATTTTAGCATCTCAATGTGATAGATTCGCATTAGA
AGTTAGAGAATTGATGAGACCGGAAATAAATGAAATAAGTGAAGGCGTAGAAGGTCGTGTAGGAAGTAGTACTATGCCTC
AGAAGGAAAATCCAGTTACGTCAGAAAAGATTGGGGGTTTAGCTAAGGTTTTACGCTCTCTTGTTGTAGCGTCATTAGAA
AATATTCCATTATGGCACGAAAGAGATCTGACTAACAGTTCGTCAGAGAGAATAGTAATATCACACGAATTTCTAATTAT
CGACGAAATATTGGATAGTATGACAAATCTTTTAAGGAATCTAAGAGTTAACTTCGAGAATATTGAAAAGAATTTGAACT
TGACTAAAGGACAAATAATGGCAGAAAGTCTGATGATAAACTTAACCTTAAAAGGCATGAAAAGGCACGAGGCTCATCAG
TACGTTAACAAACTAACGTCAAAAGTAAGGCAAACTGGAAAAACGTTATTAGAGGTTTGTCTAGAGGATAATTTGATTCT
AAGTTATTTCTCTAAGCAAGAACTTGAGAACGTATTTAACCCTAAAAACTACCTAGGATCTTATGATAAGTTAATAGAGA
GAGCTATAGGCTATGCAAAAGCAATAATTAAAGGTTCAGAAATATGA

Upstream 100 bases:

>100_bases
TTTATTATCTGTTATTATCATAGTTTGAAAACCTTCCTTTTTAGCCCCATGCAAGATTTGTAATGCAGAATGACTACCGA
TTGTAGCAATTATCATTGTT

Downstream 100 bases:

>100_bases
GAAGCTCAGAATAGATGAAGCAAAGTTCATATTTTAAGCTTTAAATAATCCACTAAATATATAGTAAAATGATGCCGCCG
TAGCTCAGCCTGGTTAGAGC

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MRLQYKLINVEHTLQLAMEDGICPLEWRYGSNEMRKLFSRGEILNRRIQVEIALLKALSKYGLVKEEDIQKVEKASLTIR
PEEVDELESKIGHDVMAMVVLMAERSGDSGRYVHFGATSYDIVDTAYALIFRDAIRLVKRKIMEILEIMISLAIKYKDLV
MVGRTHGQHALPITLGFKIANYIYEFSRSLERLNEVEKRIVKGKIAGAVGTMAAWGNIGLEIEENIMKLLELRPHEISTQ
VAPRDAFAELTSDLAILASQCDRFALEVRELMRPEINEISEGVEGRVGSSTMPQKENPVTSEKIGGLAKVLRSLVVASLE
NIPLWHERDLTNSSSERIVISHEFLIIDEILDSMTNLLRNLRVNFENIEKNLNLTKGQIMAESLMINLTLKGMKRHEAHQ
YVNKLTSKVRQTGKTLLEVCLEDNLILSYFSKQELENVFNPKNYLGSYDKLIERAIGYAKAIIKGSEI

Sequences:

>Translated_468_residues
MRLQYKLINVEHTLQLAMEDGICPLEWRYGSNEMRKLFSRGEILNRRIQVEIALLKALSKYGLVKEEDIQKVEKASLTIR
PEEVDELESKIGHDVMAMVVLMAERSGDSGRYVHFGATSYDIVDTAYALIFRDAIRLVKRKIMEILEIMISLAIKYKDLV
MVGRTHGQHALPITLGFKIANYIYEFSRSLERLNEVEKRIVKGKIAGAVGTMAAWGNIGLEIEENIMKLLELRPHEISTQ
VAPRDAFAELTSDLAILASQCDRFALEVRELMRPEINEISEGVEGRVGSSTMPQKENPVTSEKIGGLAKVLRSLVVASLE
NIPLWHERDLTNSSSERIVISHEFLIIDEILDSMTNLLRNLRVNFENIEKNLNLTKGQIMAESLMINLTLKGMKRHEAHQ
YVNKLTSKVRQTGKTLLEVCLEDNLILSYFSKQELENVFNPKNYLGSYDKLIERAIGYAKAIIKGSEI
>Mature_468_residues
MRLQYKLINVEHTLQLAMEDGICPLEWRYGSNEMRKLFSRGEILNRRIQVEIALLKALSKYGLVKEEDIQKVEKASLTIR
PEEVDELESKIGHDVMAMVVLMAERSGDSGRYVHFGATSYDIVDTAYALIFRDAIRLVKRKIMEILEIMISLAIKYKDLV
MVGRTHGQHALPITLGFKIANYIYEFSRSLERLNEVEKRIVKGKIAGAVGTMAAWGNIGLEIEENIMKLLELRPHEISTQ
VAPRDAFAELTSDLAILASQCDRFALEVRELMRPEINEISEGVEGRVGSSTMPQKENPVTSEKIGGLAKVLRSLVVASLE
NIPLWHERDLTNSSSERIVISHEFLIIDEILDSMTNLLRNLRVNFENIEKNLNLTKGQIMAESLMINLTLKGMKRHEAHQ
YVNKLTSKVRQTGKTLLEVCLEDNLILSYFSKQELENVFNPKNYLGSYDKLIERAIGYAKAIIKGSEI

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=450, Percent_Identity=26.8888888888889, Blast_Score=139, Evalue=8e-33,
Organism=Homo sapiens, GI183227688, Length=384, Percent_Identity=27.34375, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI31541964, Length=325, Percent_Identity=24.6153846153846, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI68303542, Length=325, Percent_Identity=24.6153846153846, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI19743875, Length=302, Percent_Identity=23.841059602649, Blast_Score=66, Evalue=6e-11,
Organism=Escherichia coli, GI1787376, Length=464, Percent_Identity=25.6465517241379, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1790398, Length=437, Percent_Identity=24.7139588100687, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17508577, Length=375, Percent_Identity=26.1333333333333, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI32564234, Length=306, Percent_Identity=26.797385620915, Blast_Score=97, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6323391, Length=454, Percent_Identity=25.7709251101322, Blast_Score=134, Evalue=5e-32,
Organism=Drosophila melanogaster, GI24647570, Length=445, Percent_Identity=26.9662921348315, Blast_Score=126, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24640177, Length=308, Percent_Identity=26.6233766233766, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24640179, Length=308, Percent_Identity=26.6233766233766, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI78710009, Length=335, Percent_Identity=25.0746268656716, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI221473854, Length=414, Percent_Identity=21.7391304347826, Blast_Score=66, Evalue=4e-11,
Organism=Drosophila melanogaster, GI78706858, Length=414, Percent_Identity=21.7391304347826, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 53191; Mature: 53191

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLQYKLINVEHTLQLAMEDGICPLEWRYGSNEMRKLFSRGEILNRRIQVEIALLKALSK
CCEEEEEECHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YGLVKEEDIQKVEKASLTIRPEEVDELESKIGHDVMAMVVLMAERSGDSGRYVHFGATSY
HCCCCHHHHHHHHHHCCEECHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCEEEECCCCH
DIVDTAYALIFRDAIRLVKRKIMEILEIMISLAIKYKDLVMVGRTHGQHALPITLGFKIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEEEEHHHHHH
NYIYEFSRSLERLNEVEKRIVKGKIAGAVGTMAAWGNIGLEIEENIMKLLELRPHEISTQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHCCCCHHCCC
VAPRDAFAELTSDLAILASQCDRFALEVRELMRPEINEISEGVEGRVGSSTMPQKENPVT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCCCC
SEKIGGLAKVLRSLVVASLENIPLWHERDLTNSSSERIVISHEFLIIDEILDSMTNLLRN
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHH
LRVNFENIEKNLNLTKGQIMAESLMINLTLKGMKRHEAHQYVNKLTSKVRQTGKTLLEVC
HHCCHHHHHHCCCCCHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEDNLILSYFSKQELENVFNPKNYLGSYDKLIERAIGYAKAIIKGSEI
HCCCHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRLQYKLINVEHTLQLAMEDGICPLEWRYGSNEMRKLFSRGEILNRRIQVEIALLKALSK
CCEEEEEECHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YGLVKEEDIQKVEKASLTIRPEEVDELESKIGHDVMAMVVLMAERSGDSGRYVHFGATSY
HCCCCHHHHHHHHHHCCEECHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCEEEECCCCH
DIVDTAYALIFRDAIRLVKRKIMEILEIMISLAIKYKDLVMVGRTHGQHALPITLGFKIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEEEEHHHHHH
NYIYEFSRSLERLNEVEKRIVKGKIAGAVGTMAAWGNIGLEIEENIMKLLELRPHEISTQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHCCCCHHCCC
VAPRDAFAELTSDLAILASQCDRFALEVRELMRPEINEISEGVEGRVGSSTMPQKENPVT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCCCC
SEKIGGLAKVLRSLVVASLENIPLWHERDLTNSSSERIVISHEFLIIDEILDSMTNLLRN
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHH
LRVNFENIEKNLNLTKGQIMAESLMINLTLKGMKRHEAHQYVNKLTSKVRQTGKTLLEVC
HHCCHHHHHHCCCCCHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEDNLILSYFSKQELENVFNPKNYLGSYDKLIERAIGYAKAIIKGSEI
HCCCHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12622808 [H]