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The map label for this gene is 227828070

Identifier: 227828070

GI number: 227828070

Start: 1674902

End: 1675903

Strand: Direct

Name: 227828070

Synonym: M1425_1807

Alternate gene names: NA

Gene position: 1674902-1675903 (Clockwise)

Preceding gene: 227828069

Following gene: 227828071

Centisome position: 64.2

GC content: 33.43

Gene sequence:

>1002_bases
TTGAACTATAATTTTGAAGAAGAACGTGTAGTGAAGGAAATACGTGAAAAGGACGCAAAACGTGTTCTTTTACAATTTCC
CGAAGGATTGAAGTATTTTTCTATAGATATAGTAAAGAAGTTAAAGGAAAAGCTACCTAACGTTGATTTTATAATATCTG
GTGAACCTAGTTGGGGGGCTTGTGACATAGCTGAGGATGAAGCCCAACAAGTAAATGCTGACCTAATAATTCATTTCGGT
CATACCCCTTATACGTGGTACTATCCTAAGTTTCCTACGATATTTATTAATGCTGAAAGTAATTTGGATATTTCTGAAAG
TGTTCTTCAATCTTTAGAAAATGATATCAGTAAATATAATGGAAGAAAAATCTCATTAACAGCAACCTTACAGCACGTAA
GATTAGTGTCCAAAATTAAGTCTTTCTTAGAAGATAAGGGATACGATGTAGTCATAGGCAAGCCATCTAATAGATTTATG
TTTGATGGTCAAATTTTAGGCTGTGACTACAAAGCAGCCGAAGTAGAAGCAGATACATATGTTATTGTATCAGGTGGACT
ATTTCATGCTTTAGGTTTAGGATTAGCTACAAATAAACCGACTATAAAACTTGATCCGTATTTACAGAAAAGTGAGGATA
TAACAAACGAGGTTAGGAAGATACTAAAAGTTAGATATGGTAAAATCCTACAAGCAATGGATAAACGCACTTGGGTAATT
ATACAAGGTGTTAAGGTTGGGCAAAATAGACCAAATATGATAAAATACTTCTATGATGAGTTAACTAAAAAAGGCTATGA
TGTTTTTATAGTAACAAATAAAGTGTTAACACGAGATGTACTTAGAAACCTAGATAGAAGCTATATAGATGTATTTTTAG
TTACATCTTGCCCTAGATTGCCCATAGATGATCTATATAACTATGAAAAGCCAGTGTTAACACCTGGAGAGGCCAAAATG
ATCATAAATAATAGTCTGGAACCCTATATTTTCCCATGGTGA

Upstream 100 bases:

>100_bases
ACTTCGCAAAAGAGGCGTTAAAGATAGCATCAAAGAAGTTACCTTTGCGTACTAGAATTGAGATCATTCCATTAAGGCCC
ATAAGACAGGAGGTTCAAAG

Downstream 100 bases:

>100_bases
AGTAAATGAGAGTTCAAGGTGAATTAACCTTACCCGGAGAAGAGCTAGCAGTGATTGAAGAATTTATAGCTGGCGATGGT
ACGTATGAACAAAATGGTAT

Product: diphthamide biosynthesis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MNYNFEEERVVKEIREKDAKRVLLQFPEGLKYFSIDIVKKLKEKLPNVDFIISGEPSWGACDIAEDEAQQVNADLIIHFG
HTPYTWYYPKFPTIFINAESNLDISESVLQSLENDISKYNGRKISLTATLQHVRLVSKIKSFLEDKGYDVVIGKPSNRFM
FDGQILGCDYKAAEVEADTYVIVSGGLFHALGLGLATNKPTIKLDPYLQKSEDITNEVRKILKVRYGKILQAMDKRTWVI
IQGVKVGQNRPNMIKYFYDELTKKGYDVFIVTNKVLTRDVLRNLDRSYIDVFLVTSCPRLPIDDLYNYEKPVLTPGEAKM
IINNSLEPYIFPW

Sequences:

>Translated_333_residues
MNYNFEEERVVKEIREKDAKRVLLQFPEGLKYFSIDIVKKLKEKLPNVDFIISGEPSWGACDIAEDEAQQVNADLIIHFG
HTPYTWYYPKFPTIFINAESNLDISESVLQSLENDISKYNGRKISLTATLQHVRLVSKIKSFLEDKGYDVVIGKPSNRFM
FDGQILGCDYKAAEVEADTYVIVSGGLFHALGLGLATNKPTIKLDPYLQKSEDITNEVRKILKVRYGKILQAMDKRTWVI
IQGVKVGQNRPNMIKYFYDELTKKGYDVFIVTNKVLTRDVLRNLDRSYIDVFLVTSCPRLPIDDLYNYEKPVLTPGEAKM
IINNSLEPYIFPW
>Mature_333_residues
MNYNFEEERVVKEIREKDAKRVLLQFPEGLKYFSIDIVKKLKEKLPNVDFIISGEPSWGACDIAEDEAQQVNADLIIHFG
HTPYTWYYPKFPTIFINAESNLDISESVLQSLENDISKYNGRKISLTATLQHVRLVSKIKSFLEDKGYDVVIGKPSNRFM
FDGQILGCDYKAAEVEADTYVIVSGGLFHALGLGLATNKPTIKLDPYLQKSEDITNEVRKILKVRYGKILQAMDKRTWVI
IQGVKVGQNRPNMIKYFYDELTKKGYDVFIVTNKVLTRDVLRNLDRSYIDVFLVTSCPRLPIDDLYNYEKPVLTPGEAKM
IINNSLEPYIFPW

Specific function: Unknown

COG id: COG1736

COG function: function code J; Diphthamide synthase subunit DPH2

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI116686106, Length=327, Percent_Identity=25.9938837920489, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17552166, Length=336, Percent_Identity=25.5952380952381, Blast_Score=103, Evalue=9e-23,
Organism=Saccharomyces cerevisiae, GI6322088, Length=330, Percent_Identity=30.3030303030303, Blast_Score=126, Evalue=5e-30,
Organism=Drosophila melanogaster, GI24662841, Length=332, Percent_Identity=28.0120481927711, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI21356957, Length=332, Percent_Identity=28.0120481927711, Blast_Score=115, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016435
- InterPro:   IPR002728
- InterPro:   IPR022428 [H]

Pfam domain/function: PF01866 Diphthamide_syn [H]

EC number: NA

Molecular weight: Translated: 38330; Mature: 38330

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS00626 RCC1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYNFEEERVVKEIREKDAKRVLLQFPEGLKYFSIDIVKKLKEKLPNVDFIISGEPSWGA
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHCCCCEEEEECCCCCCC
CDIAEDEAQQVNADLIIHFGHTPYTWYYPKFPTIFINAESNLDISESVLQSLENDISKYN
CCCCCHHHHHCCCEEEEEECCCCEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHC
GRKISLTATLQHVRLVSKIKSFLEDKGYDVVIGKPSNRFMFDGQILGCDYKAAEVEADTY
CCEEEEEHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEECCEEEECCCCCCEECCCEE
VIVSGGLFHALGLGLATNKPTIKLDPYLQKSEDITNEVRKILKVRYGKILQAMDKRTWVI
EEEECCHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
IQGVKVGQNRPNMIKYFYDELTKKGYDVFIVTNKVLTRDVLRNLDRSYIDVFLVTSCPRL
EEEEEECCCCCHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHCCHHHEEEEEECCCCCC
PIDDLYNYEKPVLTPGEAKMIINNSLEPYIFPW
CHHHHHCCCCCCCCCCCCEEEECCCCCCEEECC
>Mature Secondary Structure
MNYNFEEERVVKEIREKDAKRVLLQFPEGLKYFSIDIVKKLKEKLPNVDFIISGEPSWGA
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHCCCCEEEEECCCCCCC
CDIAEDEAQQVNADLIIHFGHTPYTWYYPKFPTIFINAESNLDISESVLQSLENDISKYN
CCCCCHHHHHCCCEEEEEECCCCEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHC
GRKISLTATLQHVRLVSKIKSFLEDKGYDVVIGKPSNRFMFDGQILGCDYKAAEVEADTY
CCEEEEEHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEECCEEEECCCCCCEECCCEE
VIVSGGLFHALGLGLATNKPTIKLDPYLQKSEDITNEVRKILKVRYGKILQAMDKRTWVI
EEEECCHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
IQGVKVGQNRPNMIKYFYDELTKKGYDVFIVTNKVLTRDVLRNLDRSYIDVFLVTSCPRL
EEEEEECCCCCHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHCCHHHEEEEEECCCCCC
PIDDLYNYEKPVLTPGEAKMIINNSLEPYIFPW
CHHHHHCCCCCCCCCCCCEEEECCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]