Click here to switch to the map view.

The map label for this gene is thiF [H]

Identifier: 227828065

GI number: 227828065

Start: 1671122

End: 1672165

Strand: Direct

Name: thiF [H]

Synonym: M1425_1802

Alternate gene names: 227828065

Gene position: 1671122-1672165 (Clockwise)

Preceding gene: 227828054

Following gene: 227828066

Centisome position: 64.06

GC content: 32.28

Gene sequence:

>1044_bases
TTGATTACATTTCTTCTTGCCTTTTCAGCTACTTGTCTTAACTTATCCAATTCTTCTATTGATATTGGAGTTCCATCACT
TCCTCCACCTTTTTCCATTGGAACAATATCAATCCCCACTTTTAGTAGGCAACACTTATTTTTAAAGATTATTCTACATT
ATAATCTTGTGGAAAGGTATTCTAGGCAGCTCTTAGTCTTAGGATTAGGAGTTCAACAGAGGTTAAATGAACTAAAGATA
TTAATTGCAGGTTGTGGAGCTTTGGGTTCTGCTATTGCGGAGCTCTTAGCTAGATTAGGAGTTGGAGAGATAACTGTAGT
TGATGCAGATGTAGTAGACGTAACTAATTTGCATAGGGTTCATTTGTTTGACGAGAACGATGTAGGTAAGCCTAAAGCAG
AGATTTGTGCTAAAAAGTTATCTCTTATAAATTCTTCAATCAAAATAAATTATATTATAGATATACTTGACGAGCAAAAT
GTTGAGAAGCTAATTTCTGATAAGGATTATGTATTTGATGCTTTAGATAGTTTATACTACAAGTTATTATTAAATGATGC
CACAGTAAAATTGCGAAAAATCCTAATATATGGAGGGATTAATGGTGAATATGGCTCTGTTAAATTAATAGATTCTAATC
AAACTTCTTGCCTATCTTGTTTTATTAATTATTCAGATCAAGATGAAATTGGAAATTCGTGCGATGTCATCGGAACTACT
CCATTAATAGTAGAATTAACAGCCACGCTTCAAGTTAATTTAATGCTTAATCATTTGAGAGGTAATCCGGATTACTCGTT
GTTTTACATAGATTCTAGAGAACTAAAAATTGAGAGGATAAACATCGAGAAGAACTTTCAATGCAAGACATGTGTACTAA
ATGAATACCCTTTTCTAAATCAGAAAATAACTAAACCTAACTGCGGAATATTTAGGGTTAATATGAAAGTAACGGAGTTA
GATAAGCCTAAAGTTTTTAAAAACATCGGGAATGCAATTTTATGTTATCCTAATATTGGATGTTTCGAGAAAAGGGGAAG
ATAA

Upstream 100 bases:

>100_bases
GCTTGAATCAGTCCTTATATGTTTGAATATTAATGTAGTTAGTATCTCTATGCTACTTAGAGAAGATCCTACATGGATTG
TCTGATCATAAAACAGCATT

Downstream 100 bases:

>100_bases
TGGATAAGAAATACATTGCGGCGATTGTTCTTCCATTTGTGGTTATTATAATCTATGCATACATATTTAAAATCAATGTA
GTATATGTATTAAAGGAGGA

Product: UBA/THIF-type NAD/FAD binding protein

Products: ThiS-COSH; AMP; ThiS-COAMP; pyrophosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MITFLLAFSATCLNLSNSSIDIGVPSLPPPFSIGTISIPTFSRQHLFLKIILHYNLVERYSRQLLVLGLGVQQRLNELKI
LIAGCGALGSAIAELLARLGVGEITVVDADVVDVTNLHRVHLFDENDVGKPKAEICAKKLSLINSSIKINYIIDILDEQN
VEKLISDKDYVFDALDSLYYKLLLNDATVKLRKILIYGGINGEYGSVKLIDSNQTSCLSCFINYSDQDEIGNSCDVIGTT
PLIVELTATLQVNLMLNHLRGNPDYSLFYIDSRELKIERINIEKNFQCKTCVLNEYPFLNQKITKPNCGIFRVNMKVTEL
DKPKVFKNIGNAILCYPNIGCFEKRGR

Sequences:

>Translated_347_residues
MITFLLAFSATCLNLSNSSIDIGVPSLPPPFSIGTISIPTFSRQHLFLKIILHYNLVERYSRQLLVLGLGVQQRLNELKI
LIAGCGALGSAIAELLARLGVGEITVVDADVVDVTNLHRVHLFDENDVGKPKAEICAKKLSLINSSIKINYIIDILDEQN
VEKLISDKDYVFDALDSLYYKLLLNDATVKLRKILIYGGINGEYGSVKLIDSNQTSCLSCFINYSDQDEIGNSCDVIGTT
PLIVELTATLQVNLMLNHLRGNPDYSLFYIDSRELKIERINIEKNFQCKTCVLNEYPFLNQKITKPNCGIFRVNMKVTEL
DKPKVFKNIGNAILCYPNIGCFEKRGR
>Mature_347_residues
MITFLLAFSATCLNLSNSSIDIGVPSLPPPFSIGTISIPTFSRQHLFLKIILHYNLVERYSRQLLVLGLGVQQRLNELKI
LIAGCGALGSAIAELLARLGVGEITVVDADVVDVTNLHRVHLFDENDVGKPKAEICAKKLSLINSSIKINYIIDILDEQN
VEKLISDKDYVFDALDSLYYKLLLNDATVKLRKILIYGGINGEYGSVKLIDSNQTSCLSCFINYSDQDEIGNSCDVIGTT
PLIVELTATLQVNLMLNHLRGNPDYSLFYIDSRELKIERINIEKNFQCKTCVLNEYPFLNQKITKPNCGIFRVNMKVTEL
DKPKVFKNIGNAILCYPNIGCFEKRGR

Specific function: Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein ThiS [H]

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesA/moeB/thiF family [H]

Homologues:

Organism=Homo sapiens, GI7657339, Length=188, Percent_Identity=32.4468085106383, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI38045948, Length=161, Percent_Identity=31.6770186335404, Blast_Score=79, Evalue=8e-15,
Organism=Homo sapiens, GI23510338, Length=167, Percent_Identity=29.3413173652695, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI23510340, Length=167, Percent_Identity=29.3413173652695, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI150417996, Length=163, Percent_Identity=28.8343558282209, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI38045942, Length=162, Percent_Identity=29.6296296296296, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI38045944, Length=162, Percent_Identity=29.6296296296296, Blast_Score=66, Evalue=4e-11,
Organism=Escherichia coli, GI87082356, Length=238, Percent_Identity=28.1512605042017, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787048, Length=239, Percent_Identity=25.9414225941423, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1789177, Length=139, Percent_Identity=33.0935251798561, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17540406, Length=241, Percent_Identity=31.9502074688797, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI86565433, Length=179, Percent_Identity=25.1396648044693, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI86565431, Length=179, Percent_Identity=25.1396648044693, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6321903, Length=218, Percent_Identity=28.8990825688073, Blast_Score=85, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6322639, Length=164, Percent_Identity=32.3170731707317, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6320598, Length=163, Percent_Identity=27.6073619631902, Blast_Score=74, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6325323, Length=167, Percent_Identity=30.5389221556886, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24582879, Length=198, Percent_Identity=30.3030303030303, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI28573937, Length=162, Percent_Identity=29.6296296296296, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00899 ThiF [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 38801; Mature: 38801

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITFLLAFSATCLNLSNSSIDIGVPSLPPPFSIGTISIPTFSRQHLFLKIILHYNLVERY
CEEEEEEEEEEEEECCCCEEEECCCCCCCCCCCCEEECCCCCCCCEEEEHHHHHHHHHHH
SRQLLVLGLGVQQRLNELKILIAGCGALGSAIAELLARLGVGEITVVDADVVDVTNLHRV
CCEEEEEECCHHHHHHHEEEEEEECCHHHHHHHHHHHHCCCCEEEEEECCEECCCCCEEE
HLFDENDVGKPKAEICAKKLSLINSSIKINYIIDILDEQNVEKLISDKDYVFDALDSLYY
EEECCCCCCCCHHHHHHHHHHHHCCCEEEEEEEEECCCCCHHHHHCCCHHHHHHHHHHHH
KLLLNDATVKLRKILIYGGINGEYGSVKLIDSNQTSCLSCFINYSDQDEIGNSCDVIGTT
HHHHCCCCEEEEEEEEEECCCCCCCEEEEEECCCCEEEEEEECCCCHHHCCCCCCEEECC
PLIVELTATLQVNLMLNHLRGNPDYSLFYIDSRELKIERINIEKNFQCKTCVLNEYPFLN
CEEEEEEEEEEEEEEEEECCCCCCEEEEEEECCEEEEEEECCCCCCEEEEEEECCCCCCC
QKITKPNCGIFRVNMKVTELDKPKVFKNIGNAILCYPNIGCFEKRGR
CCCCCCCCCEEEEEEEEEECCCCHHHHHCCCEEEEECCCCCCCCCCC
>Mature Secondary Structure
MITFLLAFSATCLNLSNSSIDIGVPSLPPPFSIGTISIPTFSRQHLFLKIILHYNLVERY
CEEEEEEEEEEEEECCCCEEEECCCCCCCCCCCCEEECCCCCCCCEEEEHHHHHHHHHHH
SRQLLVLGLGVQQRLNELKILIAGCGALGSAIAELLARLGVGEITVVDADVVDVTNLHRV
CCEEEEEECCHHHHHHHEEEEEEECCHHHHHHHHHHHHCCCCEEEEEECCEECCCCCEEE
HLFDENDVGKPKAEICAKKLSLINSSIKINYIIDILDEQNVEKLISDKDYVFDALDSLYY
EEECCCCCCCCHHHHHHHHHHHHCCCEEEEEEEEECCCCCHHHHHCCCHHHHHHHHHHHH
KLLLNDATVKLRKILIYGGINGEYGSVKLIDSNQTSCLSCFINYSDQDEIGNSCDVIGTT
HHHHCCCCEEEEEEEEEECCCCCCCEEEEEECCCCEEEEEEECCCCHHHCCCCCCEEECC
PLIVELTATLQVNLMLNHLRGNPDYSLFYIDSRELKIERINIEKNFQCKTCVLNEYPFLN
CEEEEEEEEEEEEEEEEECCCCCCEEEEEEECCEEEEEEECCCCCCEEEEEEECCCCCCC
QKITKPNCGIFRVNMKVTELDKPKVFKNIGNAILCYPNIGCFEKRGR
CCCCCCCCCEEEEEEEEEECCCCHHHHHCCCEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ThiS-COAMP; L-cysteine; ThiS protein; ATP [C]

Specific reaction: ThiS-COAMP + L-cysteine = ThiS-COSH + AMP ThiS protein + ATP = ThiS-COAMP + pyrophosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]