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The map label for this gene is thrS2 [H]

Identifier: 227827985

GI number: 227827985

Start: 1597475

End: 1598635

Strand: Direct

Name: thrS2 [H]

Synonym: M1425_1721

Alternate gene names: 227827985

Gene position: 1597475-1598635 (Clockwise)

Preceding gene: 227827984

Following gene: 227827986

Centisome position: 61.23

GC content: 37.38

Gene sequence:

>1161_bases
GTGATAATACTATTTATTCACGCGTCGGACTTCTCGTTTAATGTAAAGGAAAAAGCCATTAAGGAACCAGAAGAGGCTAA
ATTAAAGTCAATAGAGTTAAAGAACGTTCTGGTCTGTTTTACTACAGTGGAGAAAGGTGATGATGAGGAGATTTTAAACG
AGGCCATAAACGATATCTTAGACGTTTACAGTAAGGTGAAAGCAGCTTCCGTGGTTATTTATCCTTATGCTCACTTATCT
TCTAACTTAGCAAATCCAGACACTGCCATAAAGGTGCTTGAATCGTTGGAGAACTTATTAAAGGATAAGGTAAAGGTTTA
TAGGGCACCATTTGGTTGGTACAAGGCGTTTTCAATATCTTGCTATGGCCATCCCTTAAGCGAGTTATCTAGGAGAATAA
GAAAGGGTGAGGAGTTGGAGAAAAGTGAGGAGTTGAAGTACTGTGAAAAGTTCGGATTTCCTACCTCTGCTGAAAGCGCG
TTTATGAGGAGAGCAGTAATAGGATATTTAAGGAACATATTTCAACCAATTTTCGAATCCGAGAATAATGAAAACGTAAG
TAATGGCGAAATGAGTATACTTTATCGAAATGTTGAGAGCGGAAGAATTCTCCCATGTATTAATGAGAATCCTAGGGTAA
TAGTAGTGTACGGTGGTGTTAAGGAGTTAGATTTTCCCAAAGAGATTATTGATAGCAAAAATAGAATTAAGGTCTGGTGG
GTAAACGAGAGTAGAACTTATGTTGACGTTGGAAGATTGGTATATTACTTTATTTTAGAATCGATTAGGAAACAGCCTCC
TACTTTGCCAGATTGGCTTAATCCAATTCAAGTTAGACTGTTACCAGTAAAGAAGGACTTCTTAGATTTCTCAATACAAG
TTGCGGAAAGGTTAAGAAAGGAGGGAATAAGAGTTAATATTGATGATTTGGATGATAGCTTAGGAAATAAGATAAGGAGA
GCTGGGACTGAGTGGATACCTTTCGTCATAATTATAGGAGAAAGGGAAGTGAAGACTAGTACGCTTACTGTAAAAATTAG
AGCGAATAATGAGCAAAAGAGTATGAGTGTAGAGGAGTTAGTTAAGGAGATAAAGGATGAGGTTAAGGAAAGGCAGAACT
TACCACTTTATTATAGTCTCTATAAGCACGAAAACCGCTGA

Upstream 100 bases:

>100_bases
TCATTGGCTGATTTAATAAAGCAAGAGACTGGAGTTGAAGTAATAATTCCGGAAAATGGTCAAGAAATTAGTTTATAAAC
GTTTGAAGTAGACGTAATTT

Downstream 100 bases:

>100_bases
GTTTGCGATGTGAATGAAGTACGAAATTTTAGGAGGAAGCTAGACAAATATTTCACGCTGCAATTGGATACTAACCCTTT
AATGCCTCTCTAGTAAGAGA

Product: threonyl-tRNA synthetase editing domain protein

Products: NA

Alternate protein names: Threonine--tRNA ligase 2; ThrRS 2 [H]

Number of amino acids: Translated: 386; Mature: 386

Protein sequence:

>386_residues
MIILFIHASDFSFNVKEKAIKEPEEAKLKSIELKNVLVCFTTVEKGDDEEILNEAINDILDVYSKVKAASVVIYPYAHLS
SNLANPDTAIKVLESLENLLKDKVKVYRAPFGWYKAFSISCYGHPLSELSRRIRKGEELEKSEELKYCEKFGFPTSAESA
FMRRAVIGYLRNIFQPIFESENNENVSNGEMSILYRNVESGRILPCINENPRVIVVYGGVKELDFPKEIIDSKNRIKVWW
VNESRTYVDVGRLVYYFILESIRKQPPTLPDWLNPIQVRLLPVKKDFLDFSIQVAERLRKEGIRVNIDDLDDSLGNKIRR
AGTEWIPFVIIIGEREVKTSTLTVKIRANNEQKSMSVEELVKEIKDEVKERQNLPLYYSLYKHENR

Sequences:

>Translated_386_residues
MIILFIHASDFSFNVKEKAIKEPEEAKLKSIELKNVLVCFTTVEKGDDEEILNEAINDILDVYSKVKAASVVIYPYAHLS
SNLANPDTAIKVLESLENLLKDKVKVYRAPFGWYKAFSISCYGHPLSELSRRIRKGEELEKSEELKYCEKFGFPTSAESA
FMRRAVIGYLRNIFQPIFESENNENVSNGEMSILYRNVESGRILPCINENPRVIVVYGGVKELDFPKEIIDSKNRIKVWW
VNESRTYVDVGRLVYYFILESIRKQPPTLPDWLNPIQVRLLPVKKDFLDFSIQVAERLRKEGIRVNIDDLDDSLGNKIRR
AGTEWIPFVIIIGEREVKTSTLTVKIRANNEQKSMSVEELVKEIKDEVKERQNLPLYYSLYKHENR
>Mature_386_residues
MIILFIHASDFSFNVKEKAIKEPEEAKLKSIELKNVLVCFTTVEKGDDEEILNEAINDILDVYSKVKAASVVIYPYAHLS
SNLANPDTAIKVLESLENLLKDKVKVYRAPFGWYKAFSISCYGHPLSELSRRIRKGEELEKSEELKYCEKFGFPTSAESA
FMRRAVIGYLRNIFQPIFESENNENVSNGEMSILYRNVESGRILPCINENPRVIVVYGGVKELDFPKEIIDSKNRIKVWW
VNESRTYVDVGRLVYYFILESIRKQPPTLPDWLNPIQVRLLPVKKDFLDFSIQVAERLRKEGIRVNIDDLDDSLGNKIRR
AGTEWIPFVIIIGEREVKTSTLTVKIRANNEQKSMSVEELVKEIKDEVKERQNLPLYYSLYKHENR

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1788013, Length=103, Percent_Identity=27.1844660194175, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004154
- InterPro:   IPR015011
- ProDom:   PD016189 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF08915 tRNA-Thr_ED [H]

EC number: =6.1.1.3 [H]

Molecular weight: Translated: 44737; Mature: 44737

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIILFIHASDFSFNVKEKAIKEPEEAKLKSIELKNVLVCFTTVEKGDDEEILNEAINDIL
CEEEEEEECCCCCCCHHHHHCCCHHHHHHHEECCCEEEEEEECCCCCHHHHHHHHHHHHH
DVYSKVKAASVVIYPYAHLSSNLANPDTAIKVLESLENLLKDKVKVYRAPFGWYKAFSIS
HHHHHHHHCEEEEEEHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEE
CYGHPLSELSRRIRKGEELEKSEELKYCEKFGFPTSAESAFMRRAVIGYLRNIFQPIFES
ECCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
ENNENVSNGEMSILYRNVESGRILPCINENPRVIVVYGGVKELDFPKEIIDSKNRIKVWW
CCCCCCCCCCEEEEEEECCCCCEEEEECCCCEEEEEECCCCCCCCCHHHHCCCCCEEEEE
VNESRTYVDVGRLVYYFILESIRKQPPTLPDWLNPIQVRLLPVKKDFLDFSIQVAERLRK
ECCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHCCCEEEEEEEECHHHHHHHHHHHHHHHH
EGIRVNIDDLDDSLGNKIRRAGTEWIPFVIIIGEREVKTSTLTVKIRANNEQKSMSVEEL
CCCEEEEHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEEEEEEEEECCCHHCCCHHHH
VKEIKDEVKERQNLPLYYSLYKHENR
HHHHHHHHHHHCCCCEEEEEECCCCC
>Mature Secondary Structure
MIILFIHASDFSFNVKEKAIKEPEEAKLKSIELKNVLVCFTTVEKGDDEEILNEAINDIL
CEEEEEEECCCCCCCHHHHHCCCHHHHHHHEECCCEEEEEEECCCCCHHHHHHHHHHHHH
DVYSKVKAASVVIYPYAHLSSNLANPDTAIKVLESLENLLKDKVKVYRAPFGWYKAFSIS
HHHHHHHHCEEEEEEHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEE
CYGHPLSELSRRIRKGEELEKSEELKYCEKFGFPTSAESAFMRRAVIGYLRNIFQPIFES
ECCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
ENNENVSNGEMSILYRNVESGRILPCINENPRVIVVYGGVKELDFPKEIIDSKNRIKVWW
CCCCCCCCCCEEEEEEECCCCCEEEEECCCCEEEEEECCCCCCCCCHHHHCCCCCEEEEE
VNESRTYVDVGRLVYYFILESIRKQPPTLPDWLNPIQVRLLPVKKDFLDFSIQVAERLRK
ECCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHCCCEEEEEEEECHHHHHHHHHHHHHHHH
EGIRVNIDDLDDSLGNKIRRAGTEWIPFVIIIGEREVKTSTLTVKIRANNEQKSMSVEEL
CCCEEEEHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEEEEEEEEECCCHHCCCHHHH
VKEIKDEVKERQNLPLYYSLYKHENR
HHHHHHHHHHHCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]