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The map label for this gene is cobB [H]

Identifier: 227827966

GI number: 227827966

Start: 1572647

End: 1573951

Strand: Direct

Name: cobB [H]

Synonym: M1425_1699

Alternate gene names: 227827966

Gene position: 1572647-1573951 (Clockwise)

Preceding gene: 227827961

Following gene: 227827968

Centisome position: 60.28

GC content: 32.18

Gene sequence:

>1305_bases
ATGAGAAGAATATTATTATCATCAGACAGAAGTGGATCGGGAAAAACACTTATCACATCAGCAATTATGAGAGCATTATC
TAAAAAATACAGAGTTAGAGGTTTCAAAGCTGGACCAGATTTCATAGATCCCGGGTATCATAAGATTGCCACAGGCTTTC
CTTCAATAAACTTAGACTTATGGATGATGGGGAAAAACAATGTAAAGAGAAGTTTAATAAAATATGGAAGAGAATTCGAT
ATAGGAATAATTGAAGGAGTAATGGGTCTATATGATGGTGTAGATACGCTTTACAGCACGTATGAGCTAGCTAAAGTTAC
TAAGACTCCAGTAATCTTGATAATTAATTGTTCTAATATAGGAAGTACTGTGGGTGCAATTGTAAAGGGGTTAAAATACT
ATAGAAACGATGTGAATATTCGAGGAGTCATCTTTAATAAAATAGCTTCCGAGACCCATTATAATTATTGTAGGAATGCA
GTAGAAGATGTTGAAGTTTTAGGCTATATTCCATTTGATAAGAATCTGGAAGTTAAATCCAGACATTTGGGCTTGGTCAC
GATTGAGGATAATAAAGAAGTACAAGACTTAATTAGATATGCGTCAGAGTTAGTAGAGAAATACGTTGATTTAGATAAAA
TCTATGAGATGGCTTCTGATGAAGACCTTGAAGTCGATCTTCCTAAAGATGATGAGAATAAGGGATTAAAACGAAAAATG
GCAATAGCTTACGATCCCGCTTTTAGTTTCTATTATCAAGAAAACTTAGATATATTGAGAAGCAGATATGAATTGGAATT
CTTCAGCCCACTAAATGACGAATACGTAGAAGATGCAGAAGCTATATATATCGGAGGAGGCTATCCAGAACTTCATCTAA
ATGAACTAGAAAGATCTAGCAAGACAAAAAGTTGGCTTAAAAATTCGTCTTATTCTGGAGTGAAAATCTATGCTGAGTGT
GGTGGCCTAATGTACTTGTCTAAAAATCTAATAGATGAGAATAATAAAAATTATAATATGGTGGGTATTTTTGATATTGA
CATAAAAACTAAGGATAAATTAACAATTGGATATACAGAATTAGAAGCAATTAATGAAAACTTCATAGCAAACAAAAATA
GTTTAGTTAGGGGACATGAATTTCATATATCTAAACCCATTAGCGTTAATGAAAAAGAATTTGTATTTAAAGTTAGAATA
GGGAAAGGTATTGTTGATAAATTAGATGGTGTAAAAAGCAATAATACTATTGCAAATTACTCTCACTTGCACTTTTCTAA
CTTTCAGCAGAAGATTGTTTTTTAA

Upstream 100 bases:

>100_bases
AATCTTATATTCCTTTCAAAAGATTTAAGATATATCATACTTTCAATCTAACTAAAGATTCAAAAGATTTAACTATCCTT
TAGAGAATATACTTCTAACA

Downstream 100 bases:

>100_bases
GCTCTATTTTCTTTACTCTTTCAATTAATTCAGCTACCTTGTCTATATCTTGTGAATAAAGCCTAACGTGGAATGGAAAC
TTTGATGAAGACTTTATCTC

Product: cobyrinic acid a,c-diamide synthase

Products: ADP; phosphate; Hydrogenobyrinic acid a,c diamide; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MRRILLSSDRSGSGKTLITSAIMRALSKKYRVRGFKAGPDFIDPGYHKIATGFPSINLDLWMMGKNNVKRSLIKYGREFD
IGIIEGVMGLYDGVDTLYSTYELAKVTKTPVILIINCSNIGSTVGAIVKGLKYYRNDVNIRGVIFNKIASETHYNYCRNA
VEDVEVLGYIPFDKNLEVKSRHLGLVTIEDNKEVQDLIRYASELVEKYVDLDKIYEMASDEDLEVDLPKDDENKGLKRKM
AIAYDPAFSFYYQENLDILRSRYELEFFSPLNDEYVEDAEAIYIGGGYPELHLNELERSSKTKSWLKNSSYSGVKIYAEC
GGLMYLSKNLIDENNKNYNMVGIFDIDIKTKDKLTIGYTELEAINENFIANKNSLVRGHEFHISKPISVNEKEFVFKVRI
GKGIVDKLDGVKSNNTIANYSHLHFSNFQQKIVF

Sequences:

>Translated_434_residues
MRRILLSSDRSGSGKTLITSAIMRALSKKYRVRGFKAGPDFIDPGYHKIATGFPSINLDLWMMGKNNVKRSLIKYGREFD
IGIIEGVMGLYDGVDTLYSTYELAKVTKTPVILIINCSNIGSTVGAIVKGLKYYRNDVNIRGVIFNKIASETHYNYCRNA
VEDVEVLGYIPFDKNLEVKSRHLGLVTIEDNKEVQDLIRYASELVEKYVDLDKIYEMASDEDLEVDLPKDDENKGLKRKM
AIAYDPAFSFYYQENLDILRSRYELEFFSPLNDEYVEDAEAIYIGGGYPELHLNELERSSKTKSWLKNSSYSGVKIYAEC
GGLMYLSKNLIDENNKNYNMVGIFDIDIKTKDKLTIGYTELEAINENFIANKNSLVRGHEFHISKPISVNEKEFVFKVRI
GKGIVDKLDGVKSNNTIANYSHLHFSNFQQKIVF
>Mature_434_residues
MRRILLSSDRSGSGKTLITSAIMRALSKKYRVRGFKAGPDFIDPGYHKIATGFPSINLDLWMMGKNNVKRSLIKYGREFD
IGIIEGVMGLYDGVDTLYSTYELAKVTKTPVILIINCSNIGSTVGAIVKGLKYYRNDVNIRGVIFNKIASETHYNYCRNA
VEDVEVLGYIPFDKNLEVKSRHLGLVTIEDNKEVQDLIRYASELVEKYVDLDKIYEMASDEDLEVDLPKDDENKGLKRKM
AIAYDPAFSFYYQENLDILRSRYELEFFSPLNDEYVEDAEAIYIGGGYPELHLNELERSSKTKSWLKNSSYSGVKIYAEC
GGLMYLSKNLIDENNKNYNMVGIFDIDIKTKDKLTIGYTELEAINENFIANKNSLVRGHEFHISKPISVNEKEFVFKVRI
GKGIVDKLDGVKSNNTIANYSHLHFSNFQQKIVF

Specific function: Responsible for the amidation of carboxylic groups at position A and C of either cobyrinic acid or hydrogenobrynic acid. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation [H]

COG id: COG1797

COG function: function code H; Cobyrinic acid a,c-diamide synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR004484
- InterPro:   IPR017929
- InterPro:   IPR011698 [H]

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3 [H]

EC number: 6.3.5.9

Molecular weight: Translated: 49526; Mature: 49526

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRILLSSDRSGSGKTLITSAIMRALSKKYRVRGFKAGPDFIDPGYHKIATGFPSINLDL
CCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCHHHHCCCCCCCEEE
WMMGKNNVKRSLIKYGREFDIGIIEGVMGLYDGVDTLYSTYELAKVTKTPVILIINCSNI
EEECCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCC
GSTVGAIVKGLKYYRNDVNIRGVIFNKIASETHYNYCRNAVEDVEVLGYIPFDKNLEVKS
CHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEC
RHLGLVTIEDNKEVQDLIRYASELVEKYVDLDKIYEMASDEDLEVDLPKDDENKGLKRKM
EEEEEEEEECCHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCEEEECCCCCCCCCCCEEE
AIAYDPAFSFYYQENLDILRSRYELEFFSPLNDEYVEDAEAIYIGGGYPELHLNELERSS
EEEECCCEEEEECCCHHHHHHHHCEEEECCCCHHHHCCCCEEEEECCCCCCHHHHHHHHH
KTKSWLKNSSYSGVKIYAECGGLMYLSKNLIDENNKNYNMVGIFDIDIKTKDKLTIGYTE
HHHHHHHCCCCCCEEEEEECCCEEEEHHHHHCCCCCCEEEEEEEEEEECCCCCEEECHHH
LEAINENFIANKNSLVRGHEFHISKPISVNEKEFVFKVRIGKGIVDKLDGVKSNNTIANY
HHHHCHHHCCCCCCEEECCEEEECCCCCCCCCEEEEEEEECCCHHHHHCCCCCCCCEECC
SHLHFSNFQQKIVF
CEEECCCCHHHCCC
>Mature Secondary Structure
MRRILLSSDRSGSGKTLITSAIMRALSKKYRVRGFKAGPDFIDPGYHKIATGFPSINLDL
CCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCHHHHCCCCCCCEEE
WMMGKNNVKRSLIKYGREFDIGIIEGVMGLYDGVDTLYSTYELAKVTKTPVILIINCSNI
EEECCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCC
GSTVGAIVKGLKYYRNDVNIRGVIFNKIASETHYNYCRNAVEDVEVLGYIPFDKNLEVKS
CHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEC
RHLGLVTIEDNKEVQDLIRYASELVEKYVDLDKIYEMASDEDLEVDLPKDDENKGLKRKM
EEEEEEEEECCHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCEEEECCCCCCCCCCCEEE
AIAYDPAFSFYYQENLDILRSRYELEFFSPLNDEYVEDAEAIYIGGGYPELHLNELERSS
EEEECCCEEEEECCCHHHHHHHHCEEEECCCCHHHHCCCCEEEEECCCCCCHHHHHHHHH
KTKSWLKNSSYSGVKIYAECGGLMYLSKNLIDENNKNYNMVGIFDIDIKTKDKLTIGYTE
HHHHHHHCCCCCCEEEEEECCCEEEEHHHHHCCCCCCEEEEEEEEEEECCCCCEEECHHH
LEAINENFIANKNSLVRGHEFHISKPISVNEKEFVFKVRIGKGIVDKLDGVKSNNTIANY
HHHHCHHHCCCCCCEEECCEEEECCCCCCCCCEEEEEEEECCCHHHHHCCCCCCCCEECC
SHLHFSNFQQKIVF
CEEECCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; hydrogenobyrinic acid; L-glutamine; H2O

Specific reaction: 2 ATP + hydrogenobyrinic acid + 2 L-glutamine + 2 H2O = 2 ADP + 2 phosphate + hydrogenobyrinic acid a,c-diamide + 2 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11427726 [H]