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The map label for this gene is alaXM [H]

Identifier: 227827921

GI number: 227827921

Start: 1537717

End: 1538427

Strand: Direct

Name: alaXM [H]

Synonym: M1425_1654

Alternate gene names: 227827921

Gene position: 1537717-1538427 (Clockwise)

Preceding gene: 227827918

Following gene: 227827922

Centisome position: 58.94

GC content: 36.01

Gene sequence:

>711_bases
ATGACAGAGGAACTTTACCTAAAAGACTCTTATATTAAAGAATTTGAGGGAAGAGTAGTTAAGATAGAAGATAGCTACGT
AATGCTCGATAAGACTGCTTTTTACCCAGGAGGAGGGGGTTTAGAGAACGATATAGGATTTTTAATAAATGAAAGAGGAG
AGAAGATAAATGTTACTGAGGCAAAAAGAGGAGAGAACGGGGAGATTTTACATAAGATAGATCAAAATGCTTCTCTTAAT
GTTGGCGATAAGGTTATAGGCAAAATAAATTGGGAAAGAAGATATAGAATGATGCGATTACATACTGCCTCGCATATAGT
TGCTGCATTAGCTTATAGCAAATTCGGAGCTTTAATAACTGGAGGGCATATAAGTCCAGAACAAGCTAAAGACGATTTTA
ATGTTGAAAATAAGGATACTCTTATCGAGTTAATAAACGAAGCTAATGAAATAGTCAAAAAAGGGATAGAGCTAAAGATC
TATTTTTTACCTAGAGAAGAAGCTCTAATGATACCAGCGATAGTGAAACTAGCCGGGAGAAATCCACCACAAATTCCAAT
ATGGCGTATAGTCGAGATTCCGGGAATAGACATACAAGCTGATGGCGGACCTCATGTTAACAATACCAAGGAAATAGGAG
AAATAGTACTACTTAAGGTTGAAAATAAGGGAAAAGGGAGAAAGAGAGTTTACTATACTGTTAAACCATAG

Upstream 100 bases:

>100_bases
AATCAAAGTGATATCGTTACCAATAAGAATAGATCCATTAACCTTAACTTATAAGCATAAGAATGGAATATTTATATTGC
AAGGCAATAAGTTAAACTAG

Downstream 100 bases:

>100_bases
GGAATTTATTTATTAGCTTGATCAGAATAACTATTTTTGTGTCATTAATTATTAGACTCGCTAAGTTTGCCGTAGTTGGT
GGGTTAGGGACAATTGTGAA

Product: threonyl/alanyl tRNA synthetase SAD

Products: AMP; diphosphate; L-alanyl-tRNA(Ala)

Alternate protein names: AlaX-M; Alanyl-tRNA deacylase AlaX-M [H]

Number of amino acids: Translated: 236; Mature: 235

Protein sequence:

>236_residues
MTEELYLKDSYIKEFEGRVVKIEDSYVMLDKTAFYPGGGGLENDIGFLINERGEKINVTEAKRGENGEILHKIDQNASLN
VGDKVIGKINWERRYRMMRLHTASHIVAALAYSKFGALITGGHISPEQAKDDFNVENKDTLIELINEANEIVKKGIELKI
YFLPREEALMIPAIVKLAGRNPPQIPIWRIVEIPGIDIQADGGPHVNNTKEIGEIVLLKVENKGKGRKRVYYTVKP

Sequences:

>Translated_236_residues
MTEELYLKDSYIKEFEGRVVKIEDSYVMLDKTAFYPGGGGLENDIGFLINERGEKINVTEAKRGENGEILHKIDQNASLN
VGDKVIGKINWERRYRMMRLHTASHIVAALAYSKFGALITGGHISPEQAKDDFNVENKDTLIELINEANEIVKKGIELKI
YFLPREEALMIPAIVKLAGRNPPQIPIWRIVEIPGIDIQADGGPHVNNTKEIGEIVLLKVENKGKGRKRVYYTVKP
>Mature_235_residues
TEELYLKDSYIKEFEGRVVKIEDSYVMLDKTAFYPGGGGLENDIGFLINERGEKINVTEAKRGENGEILHKIDQNASLNV
GDKVIGKINWERRYRMMRLHTASHIVAALAYSKFGALITGGHISPEQAKDDFNVENKDTLIELINEANEIVKKGIELKIY
FLPREEALMIPAIVKLAGRNPPQIPIWRIVEIPGIDIQADGGPHVNNTKEIGEIVLLKVENKGKGRKRVYYTVKP

Specific function: Functions in trans to edit the amino acid moiety from incorrectly charged Ser-tRNA(Ala) [H]

COG id: COG2872

COG function: function code R; Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Editing domain AlaX-M subfamily [H]

Homologues:

Organism=Homo sapiens, GI13376886, Length=210, Percent_Identity=26.6666666666667, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI217416402, Length=210, Percent_Identity=26.6666666666667, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324911, Length=225, Percent_Identity=27.5555555555556, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: 6.1.1.7

Molecular weight: Translated: 26598; Mature: 26467

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEELYLKDSYIKEFEGRVVKIEDSYVMLDKTAFYPGGGGLENDIGFLINERGEKINVTE
CCCEEEECHHHHHHHCCCEEEEECCEEEEEEEEECCCCCCCCCCCEEEECCCCCEEEEEE
AKRGENGEILHKIDQNASLNVGDKVIGKINWERRYRMMRLHTASHIVAALAYSKFGALIT
CCCCCCCCEEEEECCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
GGHISPEQAKDDFNVENKDTLIELINEANEIVKKGIELKIYFLPREEALMIPAIVKLAGR
CCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHCC
NPPQIPIWRIVEIPGIDIQADGGPHVNNTKEIGEIVLLKVENKGKGRKRVYYTVKP
CCCCCCEEEEEEECCEEEECCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEEEECC
>Mature Secondary Structure 
TEELYLKDSYIKEFEGRVVKIEDSYVMLDKTAFYPGGGGLENDIGFLINERGEKINVTE
CCEEEECHHHHHHHCCCEEEEECCEEEEEEEEECCCCCCCCCCCEEEECCCCCEEEEEE
AKRGENGEILHKIDQNASLNVGDKVIGKINWERRYRMMRLHTASHIVAALAYSKFGALIT
CCCCCCCCEEEEECCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
GGHISPEQAKDDFNVENKDTLIELINEANEIVKKGIELKIYFLPREEALMIPAIVKLAGR
CCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHCC
NPPQIPIWRIVEIPGIDIQADGGPHVNNTKEIGEIVLLKVENKGKGRKRVYYTVKP
CCCCCCEEEEEEECCEEEECCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-alanine; tRNA(Ala)

Specific reaction: ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala)

General reaction: Aminoacylation; Esterification [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11427726 [H]