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The map label for this gene is frmA [H]

Identifier: 227827898

GI number: 227827898

Start: 1520296

End: 1521411

Strand: Direct

Name: frmA [H]

Synonym: M1425_1632

Alternate gene names: 227827898

Gene position: 1520296-1521411 (Clockwise)

Preceding gene: 227827895

Following gene: 227827900

Centisome position: 58.27

GC content: 37.81

Gene sequence:

>1116_bases
ATGATTATACTGTACCTTATACTAATGAAAGGAGCAATATTGTATAAATATAATGAGCCATTAATTATTGAGGATAATAT
TCAAATAGATGACCCTAAGGTAGGTGAAACAAAGATTAGAATTGAGGCAACTAGCTTATGTCATTCAGATGTAAATGTAT
TTGAAGGAAAAACTCCAGTCCCTCCACCAGTCATTGCGGGACATGAAATAGCTGGTGTTATAGAGGAGGTAGGAAATAAC
GTTACAGACTTTAAACCTGGCGATAGGGTTATATCAGCATTTATTCATCCCTGCGGTAAATGCAAAAATTGTATAACGGG
AAAGGAGAATTTATGTGAAGTCTTTGCGAAAAATAGACTAAATGGGACATTACTAGATGGTACAACTAGATTACACTTTA
AGGATGGAACTCCGATAAGGGCATTTTTAGGAGGAGGATTTGCAGAATACGCAATGGTACCTTATACTGCATTAACTAAA
GTTCCGGAAGATTTGGATTTAAGAAAGGTTGCAGTATTGGGTTGTGCAGGTTTAACTGCTTATGGTGCTGTAAATTCCGC
TAAAATAGAGCCTGGTGAAACAGTTGCTGTAATTGGAGTTGGCGGTGTAGGATTGTCTGTGATACAGATGTTAAAGATTG
CTGGTGCCGGAAGGATAATAGCAGTCGGGACTAGAAAATGGAAATTGGAAAAGGCGTTAGAGCTAGGAGCTAGTGATGTA
GTTAACTCTAAAGAGACTGATGTTGTTAGAGCTGTTAAGAATATTACTGGAGGAGGGCCGGATGTTGTAATAGAAGTAGC
AGGAACTACGGAAACAGTGAAAATGTCTTTAGATATGGTTAGAATAGGAGGAAAGGTAGTACTAGTGGGATTACCTCCAA
CTTCTGCTGAAATTCCAATTAGGATAGCAAGTATAGTTAGAGGGGGTATTAAAATCATAGGAGACTATGGAGGGAGGCCT
AGAGTAGATATGCCTAGACTTATTGAACTTGTAAAATTGGGAAAATATGACCCTACTGCGTTAGTGACTGGAAAGTTCAG
ATTAGAAGAAATTAACGAGGCAGTAAAATTATTGGAGCAAGGAGAGGCAATAAGAAGTTTAATAATTCCTAACTAA

Upstream 100 bases:

>100_bases
AAAATCGGATAGTAATAAACCTTTAAAGTATCGTAATCGTACCACGATATAATGAATAGTTTTCGCAGTTATAAAGGGAG
ATGTTTAGAAATGTTTAAAT

Downstream 100 bases:

>100_bases
AATGGAGCTCTGGTAGTTTTTCTTCCAATTATAAACATTATTAAATAGATGATTAACATTATTATGGTAAATACAACACC
TACTATAATTAGGTTACTTA

Product: alcohol dehydrogenase zinc-binding domain protein

Products: NA

Alternate protein names: Alcohol dehydrogenase class-3; Alcohol dehydrogenase class-III; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH [H]

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MIILYLILMKGAILYKYNEPLIIEDNIQIDDPKVGETKIRIEATSLCHSDVNVFEGKTPVPPPVIAGHEIAGVIEEVGNN
VTDFKPGDRVISAFIHPCGKCKNCITGKENLCEVFAKNRLNGTLLDGTTRLHFKDGTPIRAFLGGGFAEYAMVPYTALTK
VPEDLDLRKVAVLGCAGLTAYGAVNSAKIEPGETVAVIGVGGVGLSVIQMLKIAGAGRIIAVGTRKWKLEKALELGASDV
VNSKETDVVRAVKNITGGGPDVVIEVAGTTETVKMSLDMVRIGGKVVLVGLPPTSAEIPIRIASIVRGGIKIIGDYGGRP
RVDMPRLIELVKLGKYDPTALVTGKFRLEEINEAVKLLEQGEAIRSLIIPN

Sequences:

>Translated_371_residues
MIILYLILMKGAILYKYNEPLIIEDNIQIDDPKVGETKIRIEATSLCHSDVNVFEGKTPVPPPVIAGHEIAGVIEEVGNN
VTDFKPGDRVISAFIHPCGKCKNCITGKENLCEVFAKNRLNGTLLDGTTRLHFKDGTPIRAFLGGGFAEYAMVPYTALTK
VPEDLDLRKVAVLGCAGLTAYGAVNSAKIEPGETVAVIGVGGVGLSVIQMLKIAGAGRIIAVGTRKWKLEKALELGASDV
VNSKETDVVRAVKNITGGGPDVVIEVAGTTETVKMSLDMVRIGGKVVLVGLPPTSAEIPIRIASIVRGGIKIIGDYGGRP
RVDMPRLIELVKLGKYDPTALVTGKFRLEEINEAVKLLEQGEAIRSLIIPN
>Mature_371_residues
MIILYLILMKGAILYKYNEPLIIEDNIQIDDPKVGETKIRIEATSLCHSDVNVFEGKTPVPPPVIAGHEIAGVIEEVGNN
VTDFKPGDRVISAFIHPCGKCKNCITGKENLCEVFAKNRLNGTLLDGTTRLHFKDGTPIRAFLGGGFAEYAMVPYTALTK
VPEDLDLRKVAVLGCAGLTAYGAVNSAKIEPGETVAVIGVGGVGLSVIQMLKIAGAGRIIAVGTRKWKLEKALELGASDV
VNSKETDVVRAVKNITGGGPDVVIEVAGTTETVKMSLDMVRIGGKVVLVGLPPTSAEIPIRIASIVRGGIKIIGDYGGRP
RVDMPRLIELVKLGKYDPTALVTGKFRLEEINEAVKLLEQGEAIRSLIIPN

Specific function: Has High Formaldehyde Dehydrogenase Activity In The Presence Of Glutathione And Catalyzes The Oxidation Of Normal Alcohols In A Reaction That Is Not Gsh-Dependent. In Addition, Hemithiolacetals Other Than Those Formed From Gsh, Including Omega-Thiol Fatty

COG id: COG1062

COG function: function code C; Zn-dependent alcohol dehydrogenases, class III

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily [H]

Homologues:

Organism=Homo sapiens, GI34577061, Length=372, Percent_Identity=39.7849462365591, Blast_Score=232, Evalue=5e-61,
Organism=Homo sapiens, GI4501933, Length=372, Percent_Identity=38.9784946236559, Blast_Score=224, Evalue=9e-59,
Organism=Homo sapiens, GI4501929, Length=372, Percent_Identity=38.1720430107527, Blast_Score=220, Evalue=2e-57,
Organism=Homo sapiens, GI71565152, Length=375, Percent_Identity=36.8, Blast_Score=220, Evalue=2e-57,
Organism=Homo sapiens, GI71565154, Length=369, Percent_Identity=36.3143631436314, Blast_Score=202, Evalue=4e-52,
Organism=Homo sapiens, GI262073058, Length=369, Percent_Identity=34.9593495934959, Blast_Score=202, Evalue=5e-52,
Organism=Homo sapiens, GI71743840, Length=369, Percent_Identity=34.9593495934959, Blast_Score=202, Evalue=5e-52,
Organism=Homo sapiens, GI156523966, Length=372, Percent_Identity=34.4086021505376, Blast_Score=197, Evalue=1e-50,
Organism=Homo sapiens, GI4501939, Length=365, Percent_Identity=34.5205479452055, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI156627571, Length=351, Percent_Identity=26.4957264957265, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI194239674, Length=290, Percent_Identity=27.2413793103448, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI13236495, Length=290, Percent_Identity=27.2413793103448, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI194239676, Length=252, Percent_Identity=26.5873015873016, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI22538446, Length=164, Percent_Identity=33.5365853658537, Blast_Score=72, Evalue=8e-13,
Organism=Homo sapiens, GI22538444, Length=164, Percent_Identity=33.5365853658537, Blast_Score=72, Evalue=8e-13,
Organism=Escherichia coli, GI1786552, Length=366, Percent_Identity=35.5191256830601, Blast_Score=194, Evalue=8e-51,
Organism=Escherichia coli, GI87081918, Length=362, Percent_Identity=32.0441988950276, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1788073, Length=336, Percent_Identity=30.0595238095238, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1787863, Length=359, Percent_Identity=27.0194986072423, Blast_Score=121, Evalue=8e-29,
Organism=Escherichia coli, GI1790045, Length=350, Percent_Identity=24.8571428571429, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1788075, Length=362, Percent_Identity=30.6629834254144, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1786825, Length=379, Percent_Identity=29.023746701847, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI87082125, Length=353, Percent_Identity=25.2124645892351, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1790718, Length=327, Percent_Identity=23.8532110091743, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1788407, Length=294, Percent_Identity=27.2108843537415, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI226510992, Length=330, Percent_Identity=26.969696969697, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1786518, Length=363, Percent_Identity=26.9972451790634, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI87082401, Length=338, Percent_Identity=24.5562130177515, Blast_Score=86, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI25146526, Length=375, Percent_Identity=33.8666666666667, Blast_Score=196, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI71997431, Length=377, Percent_Identity=33.42175066313, Blast_Score=192, Evalue=3e-49,
Organism=Caenorhabditis elegans, GI17565904, Length=334, Percent_Identity=34.1317365269461, Blast_Score=174, Evalue=6e-44,
Organism=Caenorhabditis elegans, GI17562876, Length=372, Percent_Identity=27.6881720430108, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17562584, Length=363, Percent_Identity=28.6501377410468, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17562582, Length=330, Percent_Identity=28.1818181818182, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71988145, Length=358, Percent_Identity=27.6536312849162, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17562878, Length=369, Percent_Identity=29.2682926829268, Blast_Score=103, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6320033, Length=367, Percent_Identity=34.8773841961853, Blast_Score=213, Evalue=6e-56,
Organism=Saccharomyces cerevisiae, GI6323961, Length=359, Percent_Identity=30.9192200557103, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6324486, Length=366, Percent_Identity=29.7814207650273, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6323729, Length=368, Percent_Identity=29.6195652173913, Blast_Score=129, Evalue=7e-31,
Organism=Saccharomyces cerevisiae, GI6319621, Length=366, Percent_Identity=28.9617486338798, Blast_Score=124, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6322619, Length=357, Percent_Identity=28.2913165266106, Blast_Score=103, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6319955, Length=357, Percent_Identity=28.2913165266106, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6323099, Length=343, Percent_Identity=26.8221574344023, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6319258, Length=404, Percent_Identity=24.009900990099, Blast_Score=70, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6319949, Length=309, Percent_Identity=26.537216828479, Blast_Score=67, Evalue=7e-12,
Organism=Drosophila melanogaster, GI17737895, Length=372, Percent_Identity=38.4408602150538, Blast_Score=223, Evalue=2e-58,
Organism=Drosophila melanogaster, GI17137530, Length=342, Percent_Identity=27.1929824561404, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI17737897, Length=340, Percent_Identity=25.5882352941176, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014183
- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.284; =1.1.1.1 [H]

Molecular weight: Translated: 39596; Mature: 39596

Theoretical pI: Translated: 7.80; Mature: 7.80

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIILYLILMKGAILYKYNEPLIIEDNIQIDDPKVGETKIRIEATSLCHSDVNVFEGKTPV
CCEEEEEHHCCEEEEEECCCEEEECCEEECCCCCCCEEEEEEEHHHHHCCCHHCCCCCCC
PPPVIAGHEIAGVIEEVGNNVTDFKPGDRVISAFIHPCGKCKNCITGKENLCEVFAKNRL
CCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHCCC
NGTLLDGTTRLHFKDGTPIRAFLGGGFAEYAMVPYTALTKVPEDLDLRKVAVLGCAGLTA
CCEEECCEEEEEECCCCCEEHHHCCCHHHHHHCCHHHHHCCCCCCCHHHEEEHHHCCHHH
YGAVNSAKIEPGETVAVIGVGGVGLSVIQMLKIAGAGRIIAVGTRKWKLEKALELGASDV
HCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHCCHHHH
VNSKETDVVRAVKNITGGGPDVVIEVAGTTETVKMSLDMVRIGGKVVLVGLPPTSAEIPI
CCCCHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEEEEEEEECCEEEEEECCCCCCCCCE
RIASIVRGGIKIIGDYGGRPRVDMPRLIELVKLGKYDPTALVTGKFRLEEINEAVKLLEQ
EEHHHHHCCEEEEECCCCCCCCCHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHH
GEAIRSLIIPN
CHHHHHHCCCC
>Mature Secondary Structure
MIILYLILMKGAILYKYNEPLIIEDNIQIDDPKVGETKIRIEATSLCHSDVNVFEGKTPV
CCEEEEEHHCCEEEEEECCCEEEECCEEECCCCCCCEEEEEEEHHHHHCCCHHCCCCCCC
PPPVIAGHEIAGVIEEVGNNVTDFKPGDRVISAFIHPCGKCKNCITGKENLCEVFAKNRL
CCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHCCC
NGTLLDGTTRLHFKDGTPIRAFLGGGFAEYAMVPYTALTKVPEDLDLRKVAVLGCAGLTA
CCEEECCEEEEEECCCCCEEHHHCCCHHHHHHCCHHHHHCCCCCCCHHHEEEHHHCCHHH
YGAVNSAKIEPGETVAVIGVGGVGLSVIQMLKIAGAGRIIAVGTRKWKLEKALELGASDV
HCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHCCHHHH
VNSKETDVVRAVKNITGGGPDVVIEVAGTTETVKMSLDMVRIGGKVVLVGLPPTSAEIPI
CCCCHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEEEEEEEECCEEEEEECCCCCCCCCE
RIASIVRGGIKIIGDYGGRPRVDMPRLIELVKLGKYDPTALVTGKFRLEEINEAVKLLEQ
EEHHHHHCCEEEEECCCCCCCCCHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHH
GEAIRSLIIPN
CHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]