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The map label for this gene is 227827895

Identifier: 227827895

GI number: 227827895

Start: 1515850

End: 1517475

Strand: Direct

Name: 227827895

Synonym: M1425_1629

Alternate gene names: NA

Gene position: 1515850-1517475 (Clockwise)

Preceding gene: 227827892

Following gene: 227827898

Centisome position: 58.1

GC content: 38.87

Gene sequence:

>1626_bases
ATGAATACTTTTTATAGTAGGAAAGTAAAATATAATATTATGGCATGGGAGATTATATTAACCGCGGATAAAGGGTCTTT
TACAGATTATGGGGGTTCCAGTGTATTAGGTTATGTAGCTTGCATGCCATCAAGACTAGTACCCAAATTCTTTATGAATA
GATTCTTCACTCCAGATGTCCCAGTGGATTCTGAAGGAAGAGCAATAGTAGCACCGTACGCTCTTAGAAAAGTAGAAAGT
ACGCTTGTTCATGCGGGATTCGATAGCGTTGCAGTAATACCTCCGCACAGACTAGAGAAAGCAATAAACCAGAAGACTAA
AGTAGTGGGATTAACAGTTCATGATCCCTTTGGCCTAAATCCAGTTAGCTTTAAGTTAAGTATGATATTTGGAGGTGGTC
CTACATGGACAGCCAAATATTTCGAAGAATTTGGAGAAAAGATCTGGAAGCTTAAGTCAAAGTATAATTTTAAAGTAATA
GTAGGTGGGCCAGGGAGCTGGGAGTTAACCAAGGAGAATAAGGATTGGGCAGATGTTATATTCATAGGAGAAGCTGAAGC
GGATTTACCACGAGTTGTAAAATCCATAATTGATGGACAAGAAGTCCCTAAAGTTGTCTATGGCAAGAATCCAAAGGTAA
ATGAAATACCACCAATAATAAACCCTGCAAGACTAGGGGAAGTTCAAATAACCAGAGGATGTCCAAGAGGATGTCAATTC
TGTCCAATAACCCCTGAAACCTTTAGAACGATACCATTAGACGTTGTGAAGAAGGAAGTTGAAGTTAACATGAGGGCTGG
TATAAAGAGGGTTGAATTCATAACTGATGATGTCCTACTGTACGGTTCACAGAAGTTGAGGGTAAATCACGAAGCTATTA
CAAAATTGTTCACCGAAACCATGAATATGGGAGTAGATGGGATATGGTTTCCGCATATCTCTGCCCCAGCTGTTAGAAGT
AGTCCACAAACAGTAAAGACTATGTCTGAGATTGCAAGATATGATGAGGATAGAGCTGCTGCTCCAGTGGTTGGACTAGA
GAGTGGAAGTGAGAAGATACTAAGTAAGTACATGAGAGCAAAGCCATTCCCTTGGACTCCTAGAGAGTGGAAAGATGTCA
TACTTGATGCGACTGCGATAATGAACGATAATTACATCTATCCTTGTTACACTATGACAATAGGTTATCCAGAGGAGACA
AATGAAGATGTTGATCAATCAATTGATTTAGTTCAGTCCATAATTGATCATAAACTTAAAGCTTGGATATTCCCATTGCC
GGTCATACCTATGGGAGTTTCTTATATAAGAAATAATCCATTTCCAGTTTTAGAAAAAATGCCCACCAGATATTGGGATC
TACTATACATATCGTGGAAATACGATTTACAGATAACGAGAGAGATGATACCAATTCTCACTGGAGGTATCAAGAACAAA
TTCGCTCAAAGGACTGTCCAATACATGATAGACAAAATATTCGACAGCATCGAATGGGTATTTAAACAACTAAAAGAGAC
ACAAGGGAAATATGCATACACTTTCGCAAGCATCAATCTCAATAACACGACGGGAGTGATAAAGGCGATCTACTGGTTAT
TCAGGTTAGCCTTTAAACCTCTTTGA

Upstream 100 bases:

>100_bases
CTAAAAGTAATTAGACGTAATAGTTCCAAGCTTTAGTATAATTCTATATACAATAAAAAAGTTTACATAAATTAAATATT
ACTCTTTTATACGCCCTATA

Downstream 100 bases:

>100_bases
TCTTTTCTACAATTGCATCGGAAATGTTTTTCCAGACAATTTTATAACCTAGATAATCTATAATGTCAACTATATAATTT
CCATATTTGTTAACTAATGA

Product: radical SAM protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 541; Mature: 541

Protein sequence:

>541_residues
MNTFYSRKVKYNIMAWEIILTADKGSFTDYGGSSVLGYVACMPSRLVPKFFMNRFFTPDVPVDSEGRAIVAPYALRKVES
TLVHAGFDSVAVIPPHRLEKAINQKTKVVGLTVHDPFGLNPVSFKLSMIFGGGPTWTAKYFEEFGEKIWKLKSKYNFKVI
VGGPGSWELTKENKDWADVIFIGEAEADLPRVVKSIIDGQEVPKVVYGKNPKVNEIPPIINPARLGEVQITRGCPRGCQF
CPITPETFRTIPLDVVKKEVEVNMRAGIKRVEFITDDVLLYGSQKLRVNHEAITKLFTETMNMGVDGIWFPHISAPAVRS
SPQTVKTMSEIARYDEDRAAAPVVGLESGSEKILSKYMRAKPFPWTPREWKDVILDATAIMNDNYIYPCYTMTIGYPEET
NEDVDQSIDLVQSIIDHKLKAWIFPLPVIPMGVSYIRNNPFPVLEKMPTRYWDLLYISWKYDLQITREMIPILTGGIKNK
FAQRTVQYMIDKIFDSIEWVFKQLKETQGKYAYTFASINLNNTTGVIKAIYWLFRLAFKPL

Sequences:

>Translated_541_residues
MNTFYSRKVKYNIMAWEIILTADKGSFTDYGGSSVLGYVACMPSRLVPKFFMNRFFTPDVPVDSEGRAIVAPYALRKVES
TLVHAGFDSVAVIPPHRLEKAINQKTKVVGLTVHDPFGLNPVSFKLSMIFGGGPTWTAKYFEEFGEKIWKLKSKYNFKVI
VGGPGSWELTKENKDWADVIFIGEAEADLPRVVKSIIDGQEVPKVVYGKNPKVNEIPPIINPARLGEVQITRGCPRGCQF
CPITPETFRTIPLDVVKKEVEVNMRAGIKRVEFITDDVLLYGSQKLRVNHEAITKLFTETMNMGVDGIWFPHISAPAVRS
SPQTVKTMSEIARYDEDRAAAPVVGLESGSEKILSKYMRAKPFPWTPREWKDVILDATAIMNDNYIYPCYTMTIGYPEET
NEDVDQSIDLVQSIIDHKLKAWIFPLPVIPMGVSYIRNNPFPVLEKMPTRYWDLLYISWKYDLQITREMIPILTGGIKNK
FAQRTVQYMIDKIFDSIEWVFKQLKETQGKYAYTFASINLNNTTGVIKAIYWLFRLAFKPL
>Mature_541_residues
MNTFYSRKVKYNIMAWEIILTADKGSFTDYGGSSVLGYVACMPSRLVPKFFMNRFFTPDVPVDSEGRAIVAPYALRKVES
TLVHAGFDSVAVIPPHRLEKAINQKTKVVGLTVHDPFGLNPVSFKLSMIFGGGPTWTAKYFEEFGEKIWKLKSKYNFKVI
VGGPGSWELTKENKDWADVIFIGEAEADLPRVVKSIIDGQEVPKVVYGKNPKVNEIPPIINPARLGEVQITRGCPRGCQF
CPITPETFRTIPLDVVKKEVEVNMRAGIKRVEFITDDVLLYGSQKLRVNHEAITKLFTETMNMGVDGIWFPHISAPAVRS
SPQTVKTMSEIARYDEDRAAAPVVGLESGSEKILSKYMRAKPFPWTPREWKDVILDATAIMNDNYIYPCYTMTIGYPEET
NEDVDQSIDLVQSIIDHKLKAWIFPLPVIPMGVSYIRNNPFPVLEKMPTRYWDLLYISWKYDLQITREMIPILTGGIKNK
FAQRTVQYMIDKIFDSIEWVFKQLKETQGKYAYTFASINLNNTTGVIKAIYWLFRLAFKPL

Specific function: Unknown

COG id: COG1032

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR007197 [H]

Pfam domain/function: PF02310 B12-binding; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 61616; Mature: 61616

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTFYSRKVKYNIMAWEIILTADKGSFTDYGGSSVLGYVACMPSRLVPKFFMNRFFTPDV
CCCCCCCEEEEEEEEEEEEEEECCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCC
PVDSEGRAIVAPYALRKVESTLVHAGFDSVAVIPPHRLEKAINQKTKVVGLTVHDPFGLN
CCCCCCCEEECHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHCCCCEEEEEEEECCCCCC
PVSFKLSMIFGGGPTWTAKYFEEFGEKIWKLKSKYNFKVIVGGPGSWELTKENKDWADVI
CEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEECCCCCEEEEE
FIGEAEADLPRVVKSIIDGQEVPKVVYGKNPKVNEIPPIINPARLGEVQITRGCPRGCQF
EEECCCCHHHHHHHHHHCCHHCCEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCC
CPITPETFRTIPLDVVKKEVEVNMRAGIKRVEFITDDVLLYGSQKLRVNHEAITKLFTET
CCCCHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHCCEEEECCCEEEECHHHHHHHHHHH
MNMGVDGIWFPHISAPAVRSSPQTVKTMSEIARYDEDRAAAPVVGLESGSEKILSKYMRA
HHCCCCCEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHC
KPFPWTPREWKDVILDATAIMNDNYIYPCYTMTIGYPEETNEDVDQSIDLVQSIIDHKLK
CCCCCCCHHHHHHHHHHHHEECCCEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
AWIFPLPVIPMGVSYIRNNPFPVLEKMPTRYWDLLYISWKYDLQITREMIPILTGGIKNK
HHEECCCHHHCCHHHHCCCCCCHHHHCCCCEEEEEEEEEEECHHHHHHHHHHHHCHHHHH
FAQRTVQYMIDKIFDSIEWVFKQLKETQGKYAYTFASINLNNTTGVIKAIYWLFRLAFKP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHCC
L
C
>Mature Secondary Structure
MNTFYSRKVKYNIMAWEIILTADKGSFTDYGGSSVLGYVACMPSRLVPKFFMNRFFTPDV
CCCCCCCEEEEEEEEEEEEEEECCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCC
PVDSEGRAIVAPYALRKVESTLVHAGFDSVAVIPPHRLEKAINQKTKVVGLTVHDPFGLN
CCCCCCCEEECHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHCCCCEEEEEEEECCCCCC
PVSFKLSMIFGGGPTWTAKYFEEFGEKIWKLKSKYNFKVIVGGPGSWELTKENKDWADVI
CEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEECCCCCEEEEE
FIGEAEADLPRVVKSIIDGQEVPKVVYGKNPKVNEIPPIINPARLGEVQITRGCPRGCQF
EEECCCCHHHHHHHHHHCCHHCCEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCC
CPITPETFRTIPLDVVKKEVEVNMRAGIKRVEFITDDVLLYGSQKLRVNHEAITKLFTET
CCCCHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHCCEEEECCCEEEECHHHHHHHHHHH
MNMGVDGIWFPHISAPAVRSSPQTVKTMSEIARYDEDRAAAPVVGLESGSEKILSKYMRA
HHCCCCCEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHC
KPFPWTPREWKDVILDATAIMNDNYIYPCYTMTIGYPEETNEDVDQSIDLVQSIIDHKLK
CCCCCCCHHHHHHHHHHHHEECCCEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
AWIFPLPVIPMGVSYIRNNPFPVLEKMPTRYWDLLYISWKYDLQITREMIPILTGGIKNK
HHEECCCHHHCCHHHHCCCCCCHHHHCCCCEEEEEEEEEEECHHHHHHHHHHHHCHHHHH
FAQRTVQYMIDKIFDSIEWVFKQLKETQGKYAYTFASINLNNTTGVIKAIYWLFRLAFKP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHCC
L
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]