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The map label for this gene is malE [H]

Identifier: 227827345

GI number: 227827345

Start: 1009707

End: 1011284

Strand: Direct

Name: malE [H]

Synonym: M1425_1064

Alternate gene names: 227827345

Gene position: 1009707-1011284 (Clockwise)

Preceding gene: 227827343

Following gene: 227827346

Centisome position: 38.7

GC content: 39.54

Gene sequence:

>1578_bases
TTGGGTAGCAAAGTAAAAAAGATAGATTATAAGGCAATTTCAAAGACACTAGTAGCAGTAATAATAGTTGTAGTAATTGT
GATAGCCATAGGGGGCATTTATGCATATTTAAGTAGTCAACATCCATCAACCCCAATGCCGACTACCTCCTCTACATCTA
CGTCAGTGACATCAACCACAACAACCTCAAGTGTTTTAAATACTAGTAATCCTCAAGCTTTAATGCAGTTAGTTGGTCTC
TCAACTGCGCCTTCTACACCAGTTACAATAACAGTATGGAACAGTTATAGTACGTCCGAAAATCAAGCGTTTAATGAGAC
ACTAGCACAATTCGAGCAAGCATTTCCATGGATTCACGTCCAAGTAACGTATGGAGTTGGAGTTGGTACTTCCCAGTTTG
AGACTGCTGCAAAGGCTGGACAAGCTCCAATAGTATATAGAGACACTAGTGATTCTGGAGGCGCATTATTCGCCGCTGGA
CTAGTGCTAAACTTATCACAGTATTTACCTCAGAGCGTTACGTCATTATATGTACCTACTGCAATAAAAGATTGGGAATT
AAACGGCTCCTTGTACGGACTACCAGATAATGTGAATTATATTGTGATGTTCTACAACAAGAAGTTCGTCCCATATCCTC
CAAACACTACTGAACAACTAGTACAAATAGCTGAAAGCGTTAACAAAACTTATCATGTTTGGGGAATAGCATATGGAGCT
GGTGATGAATACGGATATAGATTTGCGGCATGGTTTGCTGGATTTGGCGGGCAAATATTTGCTACTAATAATGGTAAGAT
CGTTCCCAGTTTAAACAGTACTGCTATGGTTAACGCATTGAACTTCTGGTATAATTTAACCTATAATCTTAAGGTCAATT
ACTTAGCTCCTTCTACTGGTGCTGGAGGTGCTGAAGGTCAGTTATTTGTTGCTAATCAAACTGCTATAATATTTGATGGT
CCTTGGGATCTAAATGCATATCTCCAGGCATTAGGACCTAATTTAGGAGCCGCACCATTACCAGTAGTTAGCCAGACTGG
ATTAAGAGCTGCACCATTTATCGGTTCTACCGGTTTTCTAATAGCTTCACCTCAAGCAAGTGGCGCTACACAATTGCAAA
TAAAGGCAGCGCTAATATTCGTTCTCTATTTCACTAACTATCAGGCTGATTTGAGATTATGGGAAGTTGCACATGACATA
CCAGCTAATTTGCAAGCCTATAATGAGGCTCTAACCCAATTAAAGGAAGGAATGTTACAACCAACATACCTTAATCGAAT
AATGGAGGGCATTTTAGAACAAGCCCAATATGGTCAACAATTCCCCAACATACCTCAAATGGCGTATTATTGGAACTCGT
TCCATCAATACGCTAGTGAGTTCTTCGCTAATAAGATAAATTCGACTCAAGCAGCACAAGGAATGGAGCAAGCTTTTATC
CAAGCTCTTGTACAAAACGGTCTAATGTCTGCACTAATACCTTTACCCATACTTCCTCCATTCCAATATATGCTAGCGTT
AATCTCAGTGATATTAACACCAATGGTTGTGGTAATTAGTCCTAAAAAAAGATGGTGA

Upstream 100 bases:

>100_bases
ATTGGGATTAATTATCATTATGAATTATTTAGGGCAAAATTCACCAAGCTAAACTTTTATACCTTGTTAAGAAATAAATG
AATAAGTAAGGTGAATCGGC

Downstream 100 bases:

>100_bases
ACTAAGAAATGAAAAAAGAGAAGTTATTTTCTCTTTTTTATATTTTGCCCATAGTTATTGTGACACTTTTCCTCTTCGTA
TTTCCATTATTCTATTCAGT

Product: extracellular solute-binding protein family 1

Products: ADP; phosphate; maltose [Cytoplasm] [C]

Alternate protein names: MMBP; Maltodextrin-binding protein [H]

Number of amino acids: Translated: 525; Mature: 524

Protein sequence:

>525_residues
MGSKVKKIDYKAISKTLVAVIIVVVIVIAIGGIYAYLSSQHPSTPMPTTSSTSTSVTSTTTTSSVLNTSNPQALMQLVGL
STAPSTPVTITVWNSYSTSENQAFNETLAQFEQAFPWIHVQVTYGVGVGTSQFETAAKAGQAPIVYRDTSDSGGALFAAG
LVLNLSQYLPQSVTSLYVPTAIKDWELNGSLYGLPDNVNYIVMFYNKKFVPYPPNTTEQLVQIAESVNKTYHVWGIAYGA
GDEYGYRFAAWFAGFGGQIFATNNGKIVPSLNSTAMVNALNFWYNLTYNLKVNYLAPSTGAGGAEGQLFVANQTAIIFDG
PWDLNAYLQALGPNLGAAPLPVVSQTGLRAAPFIGSTGFLIASPQASGATQLQIKAALIFVLYFTNYQADLRLWEVAHDI
PANLQAYNEALTQLKEGMLQPTYLNRIMEGILEQAQYGQQFPNIPQMAYYWNSFHQYASEFFANKINSTQAAQGMEQAFI
QALVQNGLMSALIPLPILPPFQYMLALISVILTPMVVVISPKKRW

Sequences:

>Translated_525_residues
MGSKVKKIDYKAISKTLVAVIIVVVIVIAIGGIYAYLSSQHPSTPMPTTSSTSTSVTSTTTTSSVLNTSNPQALMQLVGL
STAPSTPVTITVWNSYSTSENQAFNETLAQFEQAFPWIHVQVTYGVGVGTSQFETAAKAGQAPIVYRDTSDSGGALFAAG
LVLNLSQYLPQSVTSLYVPTAIKDWELNGSLYGLPDNVNYIVMFYNKKFVPYPPNTTEQLVQIAESVNKTYHVWGIAYGA
GDEYGYRFAAWFAGFGGQIFATNNGKIVPSLNSTAMVNALNFWYNLTYNLKVNYLAPSTGAGGAEGQLFVANQTAIIFDG
PWDLNAYLQALGPNLGAAPLPVVSQTGLRAAPFIGSTGFLIASPQASGATQLQIKAALIFVLYFTNYQADLRLWEVAHDI
PANLQAYNEALTQLKEGMLQPTYLNRIMEGILEQAQYGQQFPNIPQMAYYWNSFHQYASEFFANKINSTQAAQGMEQAFI
QALVQNGLMSALIPLPILPPFQYMLALISVILTPMVVVISPKKRW
>Mature_524_residues
GSKVKKIDYKAISKTLVAVIIVVVIVIAIGGIYAYLSSQHPSTPMPTTSSTSTSVTSTTTTSSVLNTSNPQALMQLVGLS
TAPSTPVTITVWNSYSTSENQAFNETLAQFEQAFPWIHVQVTYGVGVGTSQFETAAKAGQAPIVYRDTSDSGGALFAAGL
VLNLSQYLPQSVTSLYVPTAIKDWELNGSLYGLPDNVNYIVMFYNKKFVPYPPNTTEQLVQIAESVNKTYHVWGIAYGAG
DEYGYRFAAWFAGFGGQIFATNNGKIVPSLNSTAMVNALNFWYNLTYNLKVNYLAPSTGAGGAEGQLFVANQTAIIFDGP
WDLNAYLQALGPNLGAAPLPVVSQTGLRAAPFIGSTGFLIASPQASGATQLQIKAALIFVLYFTNYQADLRLWEVAHDIP
ANLQAYNEALTQLKEGMLQPTYLNRIMEGILEQAQYGQQFPNIPQMAYYWNSFHQYASEFFANKINSTQAAQGMEQAFIQ
ALVQNGLMSALIPLPILPPFQYMLALISVILTPMVVVISPKKRW

Specific function: Involved in an abc transport system for maltotriose [H]

COG id: COG2182

COG function: function code G; Maltose-binding periplasmic proteins/domains

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

None

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 720 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006060
- InterPro:   IPR006059
- InterPro:   IPR006061 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 57099; Mature: 56968

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSKVKKIDYKAISKTLVAVIIVVVIVIAIGGIYAYLSSQHPSTPMPTTSSTSTSVTSTT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECC
TTSSVLNTSNPQALMQLVGLSTAPSTPVTITVWNSYSTSENQAFNETLAQFEQAFPWIHV
CHHHHHCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEE
QVTYGVGVGTSQFETAAKAGQAPIVYRDTSDSGGALFAAGLVLNLSQYLPQSVTSLYVPT
EEEEECCCCCHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHEECCC
AIKDWELNGSLYGLPDNVNYIVMFYNKKFVPYPPNTTEQLVQIAESVNKTYHVWGIAYGA
CEECEECCCEEEECCCCCCEEEEEECCEECCCCCCCHHHHHHHHHHHCCEEEEEEEEECC
GDEYGYRFAAWFAGFGGQIFATNNGKIVPSLNSTAMVNALNFWYNLTYNLKVNYLAPSTG
CCCCCCCHHHHHHCCCCEEEECCCCEEEECCCCHHHHHEEEEEEEEEEEEEEEEECCCCC
AGGAEGQLFVANQTAIIFDGPWDLNAYLQALGPNLGAAPLPVVSQTGLRAAPFIGSTGFL
CCCCCCEEEEECCEEEEECCCCCHHHHHHHHCCCCCCCCCCHHCCCCCCCCCCCCCCCEE
IASPQASGATQLQIKAALIFVLYFTNYQADLRLWEVAHDIPANLQAYNEALTQLKEGMLQ
EECCCCCCCEEEEHHHHEEEEHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCC
PTYLNRIMEGILEQAQYGQQFPNIPQMAYYWNSFHQYASEFFANKINSTQAAQGMEQAFI
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
QALVQNGLMSALIPLPILPPFQYMLALISVILTPMVVVISPKKRW
HHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCC
>Mature Secondary Structure 
GSKVKKIDYKAISKTLVAVIIVVVIVIAIGGIYAYLSSQHPSTPMPTTSSTSTSVTSTT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECC
TTSSVLNTSNPQALMQLVGLSTAPSTPVTITVWNSYSTSENQAFNETLAQFEQAFPWIHV
CHHHHHCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEE
QVTYGVGVGTSQFETAAKAGQAPIVYRDTSDSGGALFAAGLVLNLSQYLPQSVTSLYVPT
EEEEECCCCCHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHEECCC
AIKDWELNGSLYGLPDNVNYIVMFYNKKFVPYPPNTTEQLVQIAESVNKTYHVWGIAYGA
CEECEECCCEEEECCCCCCEEEEEECCEECCCCCCCHHHHHHHHHHHCCEEEEEEEEECC
GDEYGYRFAAWFAGFGGQIFATNNGKIVPSLNSTAMVNALNFWYNLTYNLKVNYLAPSTG
CCCCCCCHHHHHHCCCCEEEECCCCEEEECCCCHHHHHEEEEEEEEEEEEEEEEECCCCC
AGGAEGQLFVANQTAIIFDGPWDLNAYLQALGPNLGAAPLPVVSQTGLRAAPFIGSTGFL
CCCCCCEEEEECCEEEEECCCCCHHHHHHHHCCCCCCCCCCHHCCCCCCCCCCCCCCCEE
IASPQASGATQLQIKAALIFVLYFTNYQADLRLWEVAHDIPANLQAYNEALTQLKEGMLQ
EECCCCCCCEEEEHHHHEEEEHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCC
PTYLNRIMEGILEQAQYGQQFPNIPQMAYYWNSFHQYASEFFANKINSTQAAQGMEQAFI
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
QALVQNGLMSALIPLPILPPFQYMLALISVILTPMVVVISPKKRW
HHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; maltose [Periplasm]; H2O [C]

Specific reaction: ATP + maltose [Periplasm] + H2O = ADP + phosphate + maltose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12622808 [H]