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The map label for this gene is insQ [H]

Identifier: 227827269

GI number: 227827269

Start: 916260

End: 917498

Strand: Direct

Name: insQ [H]

Synonym: M1425_0967

Alternate gene names: 227827269

Gene position: 916260-917498 (Clockwise)

Preceding gene: 227827268

Following gene: 227827270

Centisome position: 35.12

GC content: 41.16

Gene sequence:

>1239_bases
ATGTGGAGAGCCAAGGAAAAGAATAACAAAATCCAACTATCATTCAAGTATAGAATCTACCCAACAAGGGAAGTAGAGGA
AAAACTCATCAAAGTAATGCAGGTTGAGGTTAAAGTATACAATACCTTGCTAGACGTGATAAACAAGGCAAGGAAAGAAG
GGAAAAAGATAACACCTAAGGATACTCAAAACATGTTGAAGGACTTGAAAATAGAGGGGAAGGAACTAGTTTACTCCAAA
GCTCTCCAAATGGTGAACAACCAATTATGGTACAACATCAATGCTCTTCACGAACTGAAGAAGAAAGGGAAGAAAGTAGG
GAAACTAAGGTACAAGAAAATCATGAAGATTATCAATTATAATCAATCTGGTTTCAAGGTAGTTGGAGACAAGTTGATCC
TCTCAAAGATAGGGGAGATAAAAGTACTCTTCCACAGACCGTTGGAAGGTGAGGTAAAAGGAGTAATAATAAAGAAGAGT
TCAACTGGTTGGTATGCTATCTTTCAGGTTGAGGTCGAAAAGAAACCTCTTGAGAAAACTGGAAAAGTAGTTGGAATAGA
CTTAGGAGTGGAGAAACTAGTAACTACCTCCGATGGTGTAGTGATAGAGAACCCCAAGGTTTTTGATAAGGTAGAGAGGA
AGACGAAGATTTTTCAACAATCACTGTCAAGGAAAAAGAAGGGGTCTAGAAACTACGAGAAGGTTAGGGAAAAACTAGCT
AAGCTTCACGAACATGTGAAGAACTTGATGAGTGATTATATTCACAAGGTGACTTCATGGCTTATAGAACAGTATGATGA
GATCTACGTGGAAGACCTTAGCGTGAAGGAAATGGTTGAGGATAGTGAAAGTAAAACTTTGAGAAAGCATATTCTTCACT
CCAACCTTTCTAAGTTTATGAGCTACCTCTCCTACAAGGCTAGTAGAGCTGGTAGGAGGGTAGTGAAAGTAGATCCGAGG
AATACTTCTAAGACGTGTGCTAAATGTGGTTATGTGGAGAAGGACTTGACTTTGGCTGACCGTGTTTTTGTATGTCCCAA
GTGTGGTTGGACTGTAGACCGTGATTATAATGCTTCTCTAAATATTCTTCGTGCGGGGTCGGGACTGCCCTTAGAGCCTG
TGGACAGGAAATCTCTGCTATACATACCCTTCTCTGAGGGTGTGTATAGTAAGTTTCCTGGAAGAAGCAGGAAATCCTCA
CCGCGAGGTGGGGATGCCCCGTCCGTAAGGGCGGGGTAG

Upstream 100 bases:

>100_bases
TTCCCACAAGTCAAGGAAAAACTATGGAAGGGACACTTCTGGTCTCCTTCTTATTTCCTAGCAACAAGCGGACAAGTAAC
ATTGGAGGTACTGAAAAAAT

Downstream 100 bases:

>100_bases
TTCACTCTCAGTGAAACTGTTATGAAGAACTCTAATATATATTACTGAGTCATCCAAATAATTATTATGGTATCAAAGGG
AAAATGTATCTTATGCGAAA

Product: transposase, IS605 OrfB family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MWRAKEKNNKIQLSFKYRIYPTREVEEKLIKVMQVEVKVYNTLLDVINKARKEGKKITPKDTQNMLKDLKIEGKELVYSK
ALQMVNNQLWYNINALHELKKKGKKVGKLRYKKIMKIINYNQSGFKVVGDKLILSKIGEIKVLFHRPLEGEVKGVIIKKS
STGWYAIFQVEVEKKPLEKTGKVVGIDLGVEKLVTTSDGVVIENPKVFDKVERKTKIFQQSLSRKKKGSRNYEKVREKLA
KLHEHVKNLMSDYIHKVTSWLIEQYDEIYVEDLSVKEMVEDSESKTLRKHILHSNLSKFMSYLSYKASRAGRRVVKVDPR
NTSKTCAKCGYVEKDLTLADRVFVCPKCGWTVDRDYNASLNILRAGSGLPLEPVDRKSLLYIPFSEGVYSKFPGRSRKSS
PRGGDAPSVRAG

Sequences:

>Translated_412_residues
MWRAKEKNNKIQLSFKYRIYPTREVEEKLIKVMQVEVKVYNTLLDVINKARKEGKKITPKDTQNMLKDLKIEGKELVYSK
ALQMVNNQLWYNINALHELKKKGKKVGKLRYKKIMKIINYNQSGFKVVGDKLILSKIGEIKVLFHRPLEGEVKGVIIKKS
STGWYAIFQVEVEKKPLEKTGKVVGIDLGVEKLVTTSDGVVIENPKVFDKVERKTKIFQQSLSRKKKGSRNYEKVREKLA
KLHEHVKNLMSDYIHKVTSWLIEQYDEIYVEDLSVKEMVEDSESKTLRKHILHSNLSKFMSYLSYKASRAGRRVVKVDPR
NTSKTCAKCGYVEKDLTLADRVFVCPKCGWTVDRDYNASLNILRAGSGLPLEPVDRKSLLYIPFSEGVYSKFPGRSRKSS
PRGGDAPSVRAG
>Mature_412_residues
MWRAKEKNNKIQLSFKYRIYPTREVEEKLIKVMQVEVKVYNTLLDVINKARKEGKKITPKDTQNMLKDLKIEGKELVYSK
ALQMVNNQLWYNINALHELKKKGKKVGKLRYKKIMKIINYNQSGFKVVGDKLILSKIGEIKVLFHRPLEGEVKGVIIKKS
STGWYAIFQVEVEKKPLEKTGKVVGIDLGVEKLVTTSDGVVIENPKVFDKVERKTKIFQQSLSRKKKGSRNYEKVREKLA
KLHEHVKNLMSDYIHKVTSWLIEQYDEIYVEDLSVKEMVEDSESKTLRKHILHSNLSKFMSYLSYKASRAGRRVVKVDPR
NTSKTCAKCGYVEKDLTLADRVFVCPKCGWTVDRDYNASLNILRAGSGLPLEPVDRKSLLYIPFSEGVYSKFPGRSRKSS
PRGGDAPSVRAG

Specific function: Required for the transposition of the insertion element (Potential) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transposase 35 family [H]

Homologues:

Organism=Escherichia coli, GI288549314, Length=365, Percent_Identity=30.6849315068493, Blast_Score=152, Evalue=4e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001959
- InterPro:   IPR010095
- InterPro:   IPR021027 [H]

Pfam domain/function: PF12323 HTH_14; PF01385 Transposase_2; PF07282 Transposase_35 [H]

EC number: NA

Molecular weight: Translated: 47394; Mature: 47394

Theoretical pI: Translated: 10.46; Mature: 10.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWRAKEKNNKIQLSFKYRIYPTREVEEKLIKVMQVEVKVYNTLLDVINKARKEGKKITPK
CCCCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
DTQNMLKDLKIEGKELVYSKALQMVNNQLWYNINALHELKKKGKKVGKLRYKKIMKIINY
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NQSGFKVVGDKLILSKIGEIKVLFHRPLEGEVKGVIIKKSSTGWYAIFQVEVEKKPLEKT
CCCCCHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEEEECCCCEEEEEEEEECCCCHHHC
GKVVGIDLGVEKLVTTSDGVVIENPKVFDKVERKTKIFQQSLSRKKKGSRNYEKVREKLA
CCEEEEECCHHHHEECCCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
KLHEHVKNLMSDYIHKVTSWLIEQYDEIYVEDLSVKEMVEDSESKTLRKHILHSNLSKFM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCHHHHHHHHHHHHHHHHHHH
SYLSYKASRAGRRVVKVDPRNTSKTCAKCGYVEKDLTLADRVFVCPKCGWTVDRDYNASL
HHHHHHHHHCCCEEEEECCCCCHHHHHHCCCCCCCCCHHHHEEECCCCCCEECCCCCCCE
NILRAGSGLPLEPVDRKSLLYIPFSEGVYSKFPGRSRKSSPRGGDAPSVRAG
EEEECCCCCCCCCCCCCEEEEEECCCCHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MWRAKEKNNKIQLSFKYRIYPTREVEEKLIKVMQVEVKVYNTLLDVINKARKEGKKITPK
CCCCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
DTQNMLKDLKIEGKELVYSKALQMVNNQLWYNINALHELKKKGKKVGKLRYKKIMKIINY
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NQSGFKVVGDKLILSKIGEIKVLFHRPLEGEVKGVIIKKSSTGWYAIFQVEVEKKPLEKT
CCCCCHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEEEECCCCEEEEEEEEECCCCHHHC
GKVVGIDLGVEKLVTTSDGVVIENPKVFDKVERKTKIFQQSLSRKKKGSRNYEKVREKLA
CCEEEEECCHHHHEECCCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
KLHEHVKNLMSDYIHKVTSWLIEQYDEIYVEDLSVKEMVEDSESKTLRKHILHSNLSKFM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCHHHHHHHHHHHHHHHHHHH
SYLSYKASRAGRRVVKVDPRNTSKTCAKCGYVEKDLTLADRVFVCPKCGWTVDRDYNASL
HHHHHHHHHCCCEEEEECCCCCHHHHHHCCCCCCCCCHHHHEEECCCCCCEECCCCCCCE
NILRAGSGLPLEPVDRKSLLYIPFSEGVYSKFPGRSRKSSPRGGDAPSVRAG
EEEECCCCCCCCCCCCCEEEEEECCCCHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]