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The map label for this gene is ygcB [C]

Identifier: 227827180

GI number: 227827180

Start: 815073

End: 816719

Strand: Direct

Name: ygcB [C]

Synonym: M1425_0861

Alternate gene names: 227827180

Gene position: 815073-816719 (Clockwise)

Preceding gene: 227827179

Following gene: 227827181

Centisome position: 31.24

GC content: 37.77

Gene sequence:

>1647_bases
ATGAGGAAAGGGATACAGAAATTCCTGGACATCCTCAACTGTCGTGATAATTCAATTTATTATGAATGTGAAGACGTAAA
CTTCATCCTAACGTTCCCAACTGGTTACGGAAAGACTACCCTTTCTTTGGAAATCGCCCAAATGATTGACTTAAAGCCCA
CCCAAAACTTCAGTAGGTTAATCCACGTTGTTCCTACTAGATCGTTGGCAAAGGATATTCAGAATTCCGCTTGCGAAAGA
GGACTAGGGTTCGCAGTTCAATACTCCTTCACTCCCTCCCACATAAAATCCCCACTTTTCTTAGCAAAGTTAATCATAAC
GACTTACGATTCGTTCTTGCTAAACTTGTACAAGGCCTCAATAGGAGAGACTTTCTCATATCACGGTCATTACGATTTGC
CCAGATTTGGAATATATACTTCTCTAGTACACTTCGATGAGTTCCACTTAATGAATGAGGGAAATTCATGGACCTCTCTG
TTAGGTGCAGTTAGGCACTTATCTAAAGTGGGAGTGAATATGATACTCTCATCAGCTACACCCAGTAAGAGCGTGGAGGA
GGAATTAATCAGAATGATGGAGAACCGGAAAGTGGTTAGGTTGGCAGTTGTAAACGAGTACGGCGAATCTGCGAAAAATA
GAAATTGCGTGAAAGTGAATGAAGGTTACTACGAGTGTAAAGTGGGAAGCGTGAAGTACATTAGCCTAGAGGTAAAGGAT
AAGATAAAGGTCCCTAATATAAACATTAATTTTCTTGATAAAGAGGATGAGGTATTAGAAGTAGTTAAGAGAAACAAGGA
TAAGAAAATTATGGTCATAGTGAACACTGTTGATAAGGCAATTACAATCTACGAAAAGTTAAGGGATTTGTCTCCTTGCT
TAATACACTCGAGATTTAAGATAGGTGATAGGGATGAGAAATTGAGAAAGGAGTGTAATATTATAATTGCAACTCAAGTC
ATTGAGGTTGGCGTTAACATTTCCTCCGAGGTGATGATATCTGAACAAGCTCCAATAACCTCTATTGTACAGAGAGTTGG
CAGACTATTAAGAGATAATAAAAAGGATGAGGGAGAACTTTACATATGGAAGAGTGGGAATTATTATCCCTACGACAAAG
ATGAAGTTGAAAATACTGTTAAGGAGTTGAAGAGCAAGAAAGACAACATATCCTTAAAGTTACCGTCAAGTTACGGAGAA
ATTGTGGATAGAATTATAAAGCCTCCAGAAGAGGACCTTGATTTATTAAAAGAGCTAGATTATATAGCGGAGAACTTATT
CGCAACTAGAGAGGACTTAGAAAAATTACTTGACAAGTACTGTACTCTAACTAACTCGTATGTTATAAACGTAGCTTATG
ATACCCCAGCTTCAGAGAGAGATCTAATTCCACTTGATGGCGATTTGGCACTTAAAATAGCTATTAGGGGTAACGATTGT
GTCTACGCTTACGTAGAGGAATACATGCCAGAGAGAAAGGTAGAGCTTGATAAGGTAAATGTAAGGGAAACTTGCGTTAA
AATAACAAAACCTTGTAGAGATTATAGGAAAGTCTTTTATGATGAAGATAAACGCTATATAATATACGCACTGAAGATAG
ATAAAGAACTTTACAATGAAGAAACTGGGTTGAGGTTGAAGAAATGA

Upstream 100 bases:

>100_bases
TGAAGGATGAGCAATCACCTCTAACCTCATGGGATATGAAGAAAATAAGAGATGCATTAGAAAAGATAAGAGTCGAGATG
GAGGAGGTATACTCACCCAC

Downstream 100 bases:

>100_bases
CTTGTTGGGCATTTTACGGAATGGAAACTTTTAAACAACACAGTTTAGGTATACTCAATTTCTTTAGGGATAACTTCTCC
TATATCATTCCAATTATATC

Product: CRISPR-associated helicase Cas3

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 548; Mature: 548

Protein sequence:

>548_residues
MRKGIQKFLDILNCRDNSIYYECEDVNFILTFPTGYGKTTLSLEIAQMIDLKPTQNFSRLIHVVPTRSLAKDIQNSACER
GLGFAVQYSFTPSHIKSPLFLAKLIITTYDSFLLNLYKASIGETFSYHGHYDLPRFGIYTSLVHFDEFHLMNEGNSWTSL
LGAVRHLSKVGVNMILSSATPSKSVEEELIRMMENRKVVRLAVVNEYGESAKNRNCVKVNEGYYECKVGSVKYISLEVKD
KIKVPNININFLDKEDEVLEVVKRNKDKKIMVIVNTVDKAITIYEKLRDLSPCLIHSRFKIGDRDEKLRKECNIIIATQV
IEVGVNISSEVMISEQAPITSIVQRVGRLLRDNKKDEGELYIWKSGNYYPYDKDEVENTVKELKSKKDNISLKLPSSYGE
IVDRIIKPPEEDLDLLKELDYIAENLFATREDLEKLLDKYCTLTNSYVINVAYDTPASERDLIPLDGDLALKIAIRGNDC
VYAYVEEYMPERKVELDKVNVRETCVKITKPCRDYRKVFYDEDKRYIIYALKIDKELYNEETGLRLKK

Sequences:

>Translated_548_residues
MRKGIQKFLDILNCRDNSIYYECEDVNFILTFPTGYGKTTLSLEIAQMIDLKPTQNFSRLIHVVPTRSLAKDIQNSACER
GLGFAVQYSFTPSHIKSPLFLAKLIITTYDSFLLNLYKASIGETFSYHGHYDLPRFGIYTSLVHFDEFHLMNEGNSWTSL
LGAVRHLSKVGVNMILSSATPSKSVEEELIRMMENRKVVRLAVVNEYGESAKNRNCVKVNEGYYECKVGSVKYISLEVKD
KIKVPNININFLDKEDEVLEVVKRNKDKKIMVIVNTVDKAITIYEKLRDLSPCLIHSRFKIGDRDEKLRKECNIIIATQV
IEVGVNISSEVMISEQAPITSIVQRVGRLLRDNKKDEGELYIWKSGNYYPYDKDEVENTVKELKSKKDNISLKLPSSYGE
IVDRIIKPPEEDLDLLKELDYIAENLFATREDLEKLLDKYCTLTNSYVINVAYDTPASERDLIPLDGDLALKIAIRGNDC
VYAYVEEYMPERKVELDKVNVRETCVKITKPCRDYRKVFYDEDKRYIIYALKIDKELYNEETGLRLKK
>Mature_548_residues
MRKGIQKFLDILNCRDNSIYYECEDVNFILTFPTGYGKTTLSLEIAQMIDLKPTQNFSRLIHVVPTRSLAKDIQNSACER
GLGFAVQYSFTPSHIKSPLFLAKLIITTYDSFLLNLYKASIGETFSYHGHYDLPRFGIYTSLVHFDEFHLMNEGNSWTSL
LGAVRHLSKVGVNMILSSATPSKSVEEELIRMMENRKVVRLAVVNEYGESAKNRNCVKVNEGYYECKVGSVKYISLEVKD
KIKVPNININFLDKEDEVLEVVKRNKDKKIMVIVNTVDKAITIYEKLRDLSPCLIHSRFKIGDRDEKLRKECNIIIATQV
IEVGVNISSEVMISEQAPITSIVQRVGRLLRDNKKDEGELYIWKSGNYYPYDKDEVENTVKELKSKKDNISLKLPSSYGE
IVDRIIKPPEEDLDLLKELDYIAENLFATREDLEKLLDKYCTLTNSYVINVAYDTPASERDLIPLDGDLALKIAIRGNDC
VYAYVEEYMPERKVELDKVNVRETCVKITKPCRDYRKVFYDEDKRYIIYALKIDKELYNEETGLRLKK

Specific function: Unknown

COG id: COG1203

COG function: function code R; Predicted helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR006474
- InterPro:   IPR014021 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 63234; Mature: 63234

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKGIQKFLDILNCRDNSIYYECEDVNFILTFPTGYGKTTLSLEIAQMIDLKPTQNFSRL
CCHHHHHHHHHHCCCCCCEEEEECCCEEEEEECCCCCCEEEEEEHHHHHCCCCCCCHHHH
IHVVPTRSLAKDIQNSACERGLGFAVQYSFTPSHIKSPLFLAKLIITTYDSFLLNLYKAS
EEECCCHHHHHHHHHHHHHCCCCEEEEEECCHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
IGETFSYHGHYDLPRFGIYTSLVHFDEFHLMNEGNSWTSLLGAVRHLSKVGVNMILSSAT
HCCCEECCCCCCCCCHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHCHHHEEECCC
PSKSVEEELIRMMENRKVVRLAVVNEYGESAKNRNCVKVNEGYYECKVGSVKYISLEVKD
CCCHHHHHHHHHHHCCCEEEEEEEHHHHHHCCCCCCEEECCCEEEEEECCEEEEEEEEEC
KIKVPNININFLDKEDEVLEVVKRNKDKKIMVIVNTVDKAITIYEKLRDLSPCLIHSRFK
CEECCCCEEEEECCHHHHHHHHHCCCCCEEEEEEECHHHHHHHHHHHHCCCHHHHHCCCC
IGDRDEKLRKECNIIIATQVIEVGVNISSEVMISEQAPITSIVQRVGRLLRDNKKDEGEL
CCCCHHHHHHHCCEEEEEEEEHHCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCE
YIWKSGNYYPYDKDEVENTVKELKSKKDNISLKLPSSYGEIVDRIIKPPEEDLDLLKELD
EEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHCCCHHHHHHHHHHH
YIAENLFATREDLEKLLDKYCTLTNSYVINVAYDTPASERDLIPLDGDLALKIAIRGNDC
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEECCCCEEEEEEEECCCE
VYAYVEEYMPERKVELDKVNVRETCVKITKPCRDYRKVFYDEDKRYIIYALKIDKELYNE
EEEEHHHHCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECHHHHCC
ETGLRLKK
CCCCEECC
>Mature Secondary Structure
MRKGIQKFLDILNCRDNSIYYECEDVNFILTFPTGYGKTTLSLEIAQMIDLKPTQNFSRL
CCHHHHHHHHHHCCCCCCEEEEECCCEEEEEECCCCCCEEEEEEHHHHHCCCCCCCHHHH
IHVVPTRSLAKDIQNSACERGLGFAVQYSFTPSHIKSPLFLAKLIITTYDSFLLNLYKAS
EEECCCHHHHHHHHHHHHHCCCCEEEEEECCHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
IGETFSYHGHYDLPRFGIYTSLVHFDEFHLMNEGNSWTSLLGAVRHLSKVGVNMILSSAT
HCCCEECCCCCCCCCHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHCHHHEEECCC
PSKSVEEELIRMMENRKVVRLAVVNEYGESAKNRNCVKVNEGYYECKVGSVKYISLEVKD
CCCHHHHHHHHHHHCCCEEEEEEEHHHHHHCCCCCCEEECCCEEEEEECCEEEEEEEEEC
KIKVPNININFLDKEDEVLEVVKRNKDKKIMVIVNTVDKAITIYEKLRDLSPCLIHSRFK
CEECCCCEEEEECCHHHHHHHHHCCCCCEEEEEEECHHHHHHHHHHHHCCCHHHHHCCCC
IGDRDEKLRKECNIIIATQVIEVGVNISSEVMISEQAPITSIVQRVGRLLRDNKKDEGEL
CCCCHHHHHHHCCEEEEEEEEHHCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCE
YIWKSGNYYPYDKDEVENTVKELKSKKDNISLKLPSSYGEIVDRIIKPPEEDLDLLKELD
EEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHCCCHHHHHHHHHHH
YIAENLFATREDLEKLLDKYCTLTNSYVINVAYDTPASERDLIPLDGDLALKIAIRGNDC
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEECCCCEEEEEEEECCCE
VYAYVEEYMPERKVELDKVNVRETCVKITKPCRDYRKVFYDEDKRYIIYALKIDKELYNE
EEEEHHHHCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECHHHHCC
ETGLRLKK
CCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]