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The map label for this gene is dgoD [H]

Identifier: 227826822

GI number: 227826822

Start: 379882

End: 381012

Strand: Direct

Name: dgoD [H]

Synonym: M1425_0436

Alternate gene names: 227826822

Gene position: 379882-381012 (Clockwise)

Preceding gene: 227826821

Following gene: 227826823

Centisome position: 14.56

GC content: 35.54

Gene sequence:

>1131_bases
ATGAAGGTAAAACCTTTTTCTTTATCTATACCCTTTAATACTAATCCTCCCTCTGATTGGAAGGATACGTGGGATGTGCA
ACTATACGTTAAAGTTGAAGATGAAGAAGATTACGGTTGGGGAGAAACATTAGTAGCGGGAAGCGGAATTATAAACTCTT
ACCTTTCCATTATAAATGATATAGTAGTACCAGTTTTGTCTAAAGTAGAATTAAGCGAACCAACAGATGTTAGGACTATT
TTAGAGAAGATTCTTTTTAGTGCTGGAAATTGCGGAGTGGTTTCCGGGGCCATAAGCGCTGTGGAAATGGCATTATGGGG
TTTAAAAGCTAGGAAAAGTAATGTGGAATTGACTAGACTAATTGGTGGTAAAGTTAGAGATTCAGTAAAAGTATACGCTA
GTTTTCCCAGATTTGGTAAAATTGATTATATTATTATTACAACTAGAAAATCTATAGAGAGAGGTTTTGATTTAGTTAAG
CTTCATCAATCTCCTTCAAGTGTTATAGATGCGGTTAAAGCTATAAGAGAGAATTATAAGGAAGTTAAAATAGCGATTGA
CCTTAATTCTCCTTTTGATAATCTAGATAAGGCTAAGGAGTTCGTGGACAAGGTTCATAAGTATGAGATTGAATGGGTAG
AGGAACCATTATGGCCTCCTAATGATTACGATTTACTTTCCAAGCTTACTGAATTTTCTCCCATACCTATTGCAGCCGGA
GAGAATGAATACTCCTTATACGGTTTTAAGAAATTGATTGAAAGTGGTATAACGTATCTACAACCAGATATTGCTAAGAT
TGGAGGGGTAAGTAAATTTCTAGAAATATTAGATTTGGCATCAAAACATAAGGTGAGAGTATTGCCTCACGATAGGCCTG
ACGCTTCTCCACTGTCCTTGATATACACGTTAAATCTCGGACTAGTAAAGAGCCAAATAGAAATGGTGGAGTATACGATT
GCGGACTTTCCCAACGACCTATTTTACTCCTTGCCAAAATTCAAAAATGGCTATATATATCCCTCAGAGAACCCTGAAGT
TATAGAGGAAAACATAGAAAAATATGGTTATAAAAATAGGCTCAGAATATTACATTTCAGTGATCTAGAAAGTAAGCTAA
CAAGGAAATAA

Upstream 100 bases:

>100_bases
TCAACTATACGATTAGGGTTGCAAATGGTAGATTAACTTCAGCTGAGCTATTACAACACGATGAGTTCTCACCAATAAAG
CTTTACATAAGTGCTTGAGA

Downstream 100 bases:

>100_bases
TCCGAAGAATTTACAGGAAAAGTATGAAGAAATCCTACAGTTTTATCTAATTTTGAAGAAATTTACACATGACCGAGAAA
AAGACAATTAAATGGTGATA

Product: mandelate racemase/muconate lactonizing enzyme related protein (MR/MLE)

Products: NA

Alternate protein names: GalD [H]

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MKVKPFSLSIPFNTNPPSDWKDTWDVQLYVKVEDEEDYGWGETLVAGSGIINSYLSIINDIVVPVLSKVELSEPTDVRTI
LEKILFSAGNCGVVSGAISAVEMALWGLKARKSNVELTRLIGGKVRDSVKVYASFPRFGKIDYIIITTRKSIERGFDLVK
LHQSPSSVIDAVKAIRENYKEVKIAIDLNSPFDNLDKAKEFVDKVHKYEIEWVEEPLWPPNDYDLLSKLTEFSPIPIAAG
ENEYSLYGFKKLIESGITYLQPDIAKIGGVSKFLEILDLASKHKVRVLPHDRPDASPLSLIYTLNLGLVKSQIEMVEYTI
ADFPNDLFYSLPKFKNGYIYPSENPEVIEENIEKYGYKNRLRILHFSDLESKLTRK

Sequences:

>Translated_376_residues
MKVKPFSLSIPFNTNPPSDWKDTWDVQLYVKVEDEEDYGWGETLVAGSGIINSYLSIINDIVVPVLSKVELSEPTDVRTI
LEKILFSAGNCGVVSGAISAVEMALWGLKARKSNVELTRLIGGKVRDSVKVYASFPRFGKIDYIIITTRKSIERGFDLVK
LHQSPSSVIDAVKAIRENYKEVKIAIDLNSPFDNLDKAKEFVDKVHKYEIEWVEEPLWPPNDYDLLSKLTEFSPIPIAAG
ENEYSLYGFKKLIESGITYLQPDIAKIGGVSKFLEILDLASKHKVRVLPHDRPDASPLSLIYTLNLGLVKSQIEMVEYTI
ADFPNDLFYSLPKFKNGYIYPSENPEVIEENIEKYGYKNRLRILHFSDLESKLTRK
>Mature_376_residues
MKVKPFSLSIPFNTNPPSDWKDTWDVQLYVKVEDEEDYGWGETLVAGSGIINSYLSIINDIVVPVLSKVELSEPTDVRTI
LEKILFSAGNCGVVSGAISAVEMALWGLKARKSNVELTRLIGGKVRDSVKVYASFPRFGKIDYIIITTRKSIERGFDLVK
LHQSPSSVIDAVKAIRENYKEVKIAIDLNSPFDNLDKAKEFVDKVHKYEIEWVEEPLWPPNDYDLLSKLTEFSPIPIAAG
ENEYSLYGFKKLIESGITYLQPDIAKIGGVSKFLEILDLASKHKVRVLPHDRPDASPLSLIYTLNLGLVKSQIEMVEYTI
ADFPNDLFYSLPKFKNGYIYPSENPEVIEENIEKYGYKNRLRILHFSDLESKLTRK

Specific function: Catalyzes the dehydration of D-galactonate to 2-keto-3- deoxy-D-galactonate [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GalD subfamily [H]

Homologues:

Organism=Escherichia coli, GI48994953, Length=311, Percent_Identity=26.3665594855305, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI1787864, Length=311, Percent_Identity=25.08038585209, Blast_Score=89, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =4.2.1.6 [H]

Molecular weight: Translated: 42647; Mature: 42647

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVKPFSLSIPFNTNPPSDWKDTWDVQLYVKVEDEEDYGWGETLVAGSGIINSYLSIIND
CCCCCEEEECCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCEEECCHHHHHHHHHHHH
IVVPVLSKVELSEPTDVRTILEKILFSAGNCGVVSGAISAVEMALWGLKARKSNVELTRL
HHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHH
IGGKVRDSVKVYASFPRFGKIDYIIITTRKSIERGFDLVKLHQSPSSVIDAVKAIRENYK
HCCHHHCCEEEEECCCCCCCEEEEEEECCHHHHCCCCEEEECCCHHHHHHHHHHHHCCCE
EVKIAIDLNSPFDNLDKAKEFVDKVHKYEIEWVEEPLWPPNDYDLLSKLTEFSPIPIAAG
EEEEEEECCCCCCHHHHHHHHHHHHHHEEHHHHHCCCCCCCCHHHHHHHHHCCCCCEECC
ENEYSLYGFKKLIESGITYLQPDIAKIGGVSKFLEILDLASKHKVRVLPHDRPDASPLSL
CCCCHHHHHHHHHHCCCHHCCCHHHHHCCHHHHHHHHHHHCCCCEEECCCCCCCCCCEEE
IYTLNLGLVKSQIEMVEYTIADFPNDLFYSLPKFKNGYIYPSENPEVIEENIEKYGYKNR
EEEECHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCEECCCCCHHHHHHHHHHCCCCCC
LRILHFSDLESKLTRK
EEEEEECCHHHHHCCC
>Mature Secondary Structure
MKVKPFSLSIPFNTNPPSDWKDTWDVQLYVKVEDEEDYGWGETLVAGSGIINSYLSIIND
CCCCCEEEECCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCEEECCHHHHHHHHHHHH
IVVPVLSKVELSEPTDVRTILEKILFSAGNCGVVSGAISAVEMALWGLKARKSNVELTRL
HHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHH
IGGKVRDSVKVYASFPRFGKIDYIIITTRKSIERGFDLVKLHQSPSSVIDAVKAIRENYK
HCCHHHCCEEEEECCCCCCCEEEEEEECCHHHHCCCCEEEECCCHHHHHHHHHHHHCCCE
EVKIAIDLNSPFDNLDKAKEFVDKVHKYEIEWVEEPLWPPNDYDLLSKLTEFSPIPIAAG
EEEEEEECCCCCCHHHHHHHHHHHHHHEEHHHHHCCCCCCCCHHHHHHHHHCCCCCEECC
ENEYSLYGFKKLIESGITYLQPDIAKIGGVSKFLEILDLASKHKVRVLPHDRPDASPLSL
CCCCHHHHHHHHHHCCCHHCCCHHHHHCCHHHHHHHHHHHCCCCEEECCCCCCCCCCEEE
IYTLNLGLVKSQIEMVEYTIADFPNDLFYSLPKFKNGYIYPSENPEVIEENIEKYGYKNR
EEEECHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCEECCCCCHHHHHHHHHHCCCCCC
LRILHFSDLESKLTRK
EEEEEECCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA