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The map label for this gene is carA2 [H]

Identifier: 227826801

GI number: 227826801

Start: 356369

End: 357730

Strand: Direct

Name: carA2 [H]

Synonym: M1425_0410

Alternate gene names: 227826801

Gene position: 356369-357730 (Clockwise)

Preceding gene: 227826800

Following gene: 227826802

Centisome position: 13.66

GC content: 37.44

Gene sequence:

>1362_bases
ATGAAGGCAATAGTAGTAGGGGCAGGGATAGGAGGCATTTCTACAGCAATATTACTTAAAAAGGTATTTGATGAAGTGAC
AGTGCTGGAAAAGAACTCAACTCCAGGTGGAAGAGCGAGATATTTTTCGGCTGGCGAATTTAGATTCGATATGGGTCCTT
CTTGGTATTTAATGCCGGAGATATTTGAGGAGTTCTTCTCTAGAATAGGACAAGAAAACTATCCAATAGTGAAAGTTAAA
CCCCCAGTCAAGGTGATTGAAGGTAAGTCTCTGGAAAAGGCTTATGGAGAGTTTACTGGAGATAACGTCGATGAGAGATA
TCTAGATAAGGTGAAGAGGTTCTATGACTTTTCTAAGAAGTTCATTAAAAAAGAGATGAGTTATTTAGACTTTCTGGATA
GGGAAGTTCTGACTAACTTCTCAGCATTGTTTAACACTCTGGATTATTTCAATTCCTTATACTTTAAGGATAGTTTACTG
AAGAAGGCTATGGGTTTCTCATCAGTATTTATAGGTGGTTCTCCGTTTAAGGTTCCAGCAATTTACGCTATGATAAATTA
CGCAATTTACGGTCAAGGAGTCTTCTATCCACATAAAGGTTTTGAAGGTTACGTTAAGAGATTATGCGAGATTGCTACGA
AAATGGGAGTGGAGTTCAAGTTTAACTTCTCAGTAAACAAGGTGAAAATTGATAATAATAAAGTAGTAGAAGTCTCATCA
GATGATAAAAAGGAGAGTGGAGATGTTTTCGTCTTCAACATGGACTACTTTTACGCTGATAGTTTATTACCGGAGGAGTA
TAAGGTCATAAGAGGTAAGAGATATAAGTTATCTCCATCAGCCATTTTAGCTTATATAGGGCTTGAGAATACTCTGGATT
TGCCTCATCACACAGTAATTATTAACGGTAACTGGAGGGAACACTTCGAATCCATAGAGAGAAATTCATTACCTAGCATA
GAGGATACTTCCTATTATGTGAGCTATAGAGGTGCAACTGACAAAGAAGCCAGAGACCTAATTATACTTATACCAGTGGG
TACTGGTACCAGCATCAGTAAAGAACTTGTAAATGCGGTTATTAAGGATTTAGAGGTTAAGATAGGGAGTAAAATAAAAG
CTAGTTATTTCAGAATTTATGGACCAGAGGACTTCAAAAGAGACTACAACGCCTTTGAAGGTACAGCGTTCGGTTTAGCT
CACACTCTAGATCAGACTGGGCCTTTTAGGCTTTCAATGAAAAACCCTAGGTTGAAGAACTTATATTATGTTGGCCAATA
TACTCAACCTGGAATAGGTGTGCCTATGGTAACTCTTTCTGCAATGATTTTAAGTAAGAAAATATTGAAGGAGTTATCGT
AG

Upstream 100 bases:

>100_bases
TAGCTCACGATATCCATCATGAAGAGGGAGAAGGTAATTGGGGATTCCTCCTTGTCATAAAGGGGTTAGATAAGATCCCT
ACAGTAAAGGATGAATAAAG

Downstream 100 bases:

>100_bases
TCTTATTCCTTATATTAGTTAGGTCCAACTAATATAGGCATTACATGCCGTGACTCTAACAAGCCTAGGCGTTGCAGACT
TCTTTAGATCTTAGCATTGT

Product: phytoene desaturase

Products: NA

Alternate protein names: Phytoene desaturase [H]

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MKAIVVGAGIGGISTAILLKKVFDEVTVLEKNSTPGGRARYFSAGEFRFDMGPSWYLMPEIFEEFFSRIGQENYPIVKVK
PPVKVIEGKSLEKAYGEFTGDNVDERYLDKVKRFYDFSKKFIKKEMSYLDFLDREVLTNFSALFNTLDYFNSLYFKDSLL
KKAMGFSSVFIGGSPFKVPAIYAMINYAIYGQGVFYPHKGFEGYVKRLCEIATKMGVEFKFNFSVNKVKIDNNKVVEVSS
DDKKESGDVFVFNMDYFYADSLLPEEYKVIRGKRYKLSPSAILAYIGLENTLDLPHHTVIINGNWREHFESIERNSLPSI
EDTSYYVSYRGATDKEARDLIILIPVGTGTSISKELVNAVIKDLEVKIGSKIKASYFRIYGPEDFKRDYNAFEGTAFGLA
HTLDQTGPFRLSMKNPRLKNLYYVGQYTQPGIGVPMVTLSAMILSKKILKELS

Sequences:

>Translated_453_residues
MKAIVVGAGIGGISTAILLKKVFDEVTVLEKNSTPGGRARYFSAGEFRFDMGPSWYLMPEIFEEFFSRIGQENYPIVKVK
PPVKVIEGKSLEKAYGEFTGDNVDERYLDKVKRFYDFSKKFIKKEMSYLDFLDREVLTNFSALFNTLDYFNSLYFKDSLL
KKAMGFSSVFIGGSPFKVPAIYAMINYAIYGQGVFYPHKGFEGYVKRLCEIATKMGVEFKFNFSVNKVKIDNNKVVEVSS
DDKKESGDVFVFNMDYFYADSLLPEEYKVIRGKRYKLSPSAILAYIGLENTLDLPHHTVIINGNWREHFESIERNSLPSI
EDTSYYVSYRGATDKEARDLIILIPVGTGTSISKELVNAVIKDLEVKIGSKIKASYFRIYGPEDFKRDYNAFEGTAFGLA
HTLDQTGPFRLSMKNPRLKNLYYVGQYTQPGIGVPMVTLSAMILSKKILKELS
>Mature_453_residues
MKAIVVGAGIGGISTAILLKKVFDEVTVLEKNSTPGGRARYFSAGEFRFDMGPSWYLMPEIFEEFFSRIGQENYPIVKVK
PPVKVIEGKSLEKAYGEFTGDNVDERYLDKVKRFYDFSKKFIKKEMSYLDFLDREVLTNFSALFNTLDYFNSLYFKDSLL
KKAMGFSSVFIGGSPFKVPAIYAMINYAIYGQGVFYPHKGFEGYVKRLCEIATKMGVEFKFNFSVNKVKIDNNKVVEVSS
DDKKESGDVFVFNMDYFYADSLLPEEYKVIRGKRYKLSPSAILAYIGLENTLDLPHHTVIINGNWREHFESIERNSLPSI
EDTSYYVSYRGATDKEARDLIILIPVGTGTSISKELVNAVIKDLEVKIGSKIKASYFRIYGPEDFKRDYNAFEGTAFGLA
HTLDQTGPFRLSMKNPRLKNLYYVGQYTQPGIGVPMVTLSAMILSKKILKELS

Specific function: This enzyme converts phytoene into zeta-carotene via the intermediary of phytofluene by the symmetrical introduction of two double bonds at the C-11 and C-11' positions of phytoene [H]

COG id: COG1233

COG function: function code Q; Phytoene dehydrogenase and related proteins

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carotenoid/retinoid oxidoreductase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002937
- InterPro:   IPR008150
- InterPro:   IPR014105 [H]

Pfam domain/function: PF01593 Amino_oxidase [H]

EC number: NA

Molecular weight: Translated: 51495; Mature: 51495

Theoretical pI: Translated: 9.22; Mature: 9.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAIVVGAGIGGISTAILLKKVFDEVTVLEKNSTPGGRARYFSAGEFRFDMGPSWYLMPE
CCEEEEECCCCHHHHHHHHHHHHHHHEEEECCCCCCCCEEEEECCCEEECCCCCEEECHH
IFEEFFSRIGQENYPIVKVKPPVKVIEGKSLEKAYGEFTGDNVDERYLDKVKRFYDFSKK
HHHHHHHHHCCCCCCEEEECCCHHEECCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
FIKKEMSYLDFLDREVLTNFSALFNTLDYFNSLYFKDSLLKKAMGFSSVFIGGSPFKVPA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHH
IYAMINYAIYGQGVFYPHKGFEGYVKRLCEIATKMGVEFKFNFSVNKVKIDNNKVVEVSS
HHHHHHHHEEECCEECCCCCHHHHHHHHHHHHHHCCCEEEEEEEEEEEEECCCEEEEECC
DDKKESGDVFVFNMDYFYADSLLPEEYKVIRGKRYKLSPSAILAYIGLENTLDLPHHTVI
CCCCCCCCEEEEECHHHHHCCCCCHHHHHHCCCCEEECHHHEEEEECCCCCCCCCCEEEE
INGNWREHFESIERNSLPSIEDTSYYVSYRGATDKEARDLIILIPVGTGTSISKELVNAV
EECCHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCCCHHHHHHHHHH
IKDLEVKIGSKIKASYFRIYGPEDFKRDYNAFEGTAFGLAHTLDQTGPFRLSMKNPRLKN
HHHHHHHCCCCEEEEEEEEECCHHHHHHCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCE
LYYVGQYTQPGIGVPMVTLSAMILSKKILKELS
EEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKAIVVGAGIGGISTAILLKKVFDEVTVLEKNSTPGGRARYFSAGEFRFDMGPSWYLMPE
CCEEEEECCCCHHHHHHHHHHHHHHHEEEECCCCCCCCEEEEECCCEEECCCCCEEECHH
IFEEFFSRIGQENYPIVKVKPPVKVIEGKSLEKAYGEFTGDNVDERYLDKVKRFYDFSKK
HHHHHHHHHCCCCCCEEEECCCHHEECCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
FIKKEMSYLDFLDREVLTNFSALFNTLDYFNSLYFKDSLLKKAMGFSSVFIGGSPFKVPA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHH
IYAMINYAIYGQGVFYPHKGFEGYVKRLCEIATKMGVEFKFNFSVNKVKIDNNKVVEVSS
HHHHHHHHEEECCEECCCCCHHHHHHHHHHHHHHCCCEEEEEEEEEEEEECCCEEEEECC
DDKKESGDVFVFNMDYFYADSLLPEEYKVIRGKRYKLSPSAILAYIGLENTLDLPHHTVI
CCCCCCCCEEEEECHHHHHCCCCCHHHHHHCCCCEEECHHHEEEEECCCCCCCCCCEEEE
INGNWREHFESIERNSLPSIEDTSYYVSYRGATDKEARDLIILIPVGTGTSISKELVNAV
EECCHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCCCHHHHHHHHHH
IKDLEVKIGSKIKASYFRIYGPEDFKRDYNAFEGTAFGLAHTLDQTGPFRLSMKNPRLKN
HHHHHHHCCCCEEEEEEEEECCHHHHHHCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCE
LYYVGQYTQPGIGVPMVTLSAMILSKKILKELS
EEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7588751 [H]