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The map label for this gene is echA8 [H]

Identifier: 227826784

GI number: 227826784

Start: 340547

End: 341290

Strand: Direct

Name: echA8 [H]

Synonym: M1425_0393

Alternate gene names: 227826784

Gene position: 340547-341290 (Clockwise)

Preceding gene: 227826783

Following gene: 227826785

Centisome position: 13.05

GC content: 37.37

Gene sequence:

>744_bases
ATGGTAAAAATCCTCTCTGAAAGAAGAAAAGACGTTTGTTGGATCACATTAAATAGACCAGAAAAGTTAAATGCCCTAGA
TAAGGAAAGCTGGAGTCTCTTAGCAAAACACTTGAGGGATTGTAATGACGATCAGTCAATTTCAGCAATAGTCTTAACTG
GCAATGGAAGAGCTTTCTCCGCAGGAGATGATATCAATGCCATGTTAGAGTTAAAGGATCAAAGAGATGCCCTAGACTTC
TTCAACGCTTTATATAGCGCGATTGAAAGTCTAGTGGATTTAAAGAAGCCATTATTATGTGCAGTTAATGGGCTTGCTTA
TGGCGGTGGTTGCGAGATACTCCTTTTCTGTGATGTGATAATTTCAGTTGACGACGCTACTTTTTCAATTCCAGAAGGAA
AGTTGGGATTAATTCCGCCAATGGCAATATCAGTAGGTTATTCAATATTGGGTAGATCAATTACTAGGCTAGCATTAACT
GGAGATTCCATAACTGCTGAAGAGGCTAAAACTATAGGGCTGGTTGATATAGTGGTCAAAAGGGAAGACCTTTATACAGA
AGTTGAGAAACAGTTAGAGAAAATCAAAAGTATTGATATGAATTCTATTATGACTATGAAGTATTGGTTGAAAAACGATA
AGGAGAAGATAAGAAATGCAGTCATGGAGTTGGCGTTGATGTCATTGGGGGATTCCGCAAAAAAGAGAATGAATGAATTC
ATAAATAGAAAACGAACACGATAA

Upstream 100 bases:

>100_bases
CAGCACATTATTCAATTTTATTTCAATCTCAAAGTTTAAAATCTTCACAGTATAATTTGAAAGCTCAACATTTAAGATAT
CCTTTGCTAAATATCATATT

Downstream 100 bases:

>100_bases
AGTTTTGACTAGATTCATCTATATAAATCTTAATATCATAATACGTTAGATAATTATCACTAAAGCTATTATGTTAAATA
ACATTATAATGCTTAATAAT

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 247; Mature: 247

Protein sequence:

>247_residues
MVKILSERRKDVCWITLNRPEKLNALDKESWSLLAKHLRDCNDDQSISAIVLTGNGRAFSAGDDINAMLELKDQRDALDF
FNALYSAIESLVDLKKPLLCAVNGLAYGGGCEILLFCDVIISVDDATFSIPEGKLGLIPPMAISVGYSILGRSITRLALT
GDSITAEEAKTIGLVDIVVKREDLYTEVEKQLEKIKSIDMNSIMTMKYWLKNDKEKIRNAVMELALMSLGDSAKKRMNEF
INRKRTR

Sequences:

>Translated_247_residues
MVKILSERRKDVCWITLNRPEKLNALDKESWSLLAKHLRDCNDDQSISAIVLTGNGRAFSAGDDINAMLELKDQRDALDF
FNALYSAIESLVDLKKPLLCAVNGLAYGGGCEILLFCDVIISVDDATFSIPEGKLGLIPPMAISVGYSILGRSITRLALT
GDSITAEEAKTIGLVDIVVKREDLYTEVEKQLEKIKSIDMNSIMTMKYWLKNDKEKIRNAVMELALMSLGDSAKKRMNEF
INRKRTR
>Mature_247_residues
MVKILSERRKDVCWITLNRPEKLNALDKESWSLLAKHLRDCNDDQSISAIVLTGNGRAFSAGDDINAMLELKDQRDALDF
FNALYSAIESLVDLKKPLLCAVNGLAYGGGCEILLFCDVIISVDDATFSIPEGKLGLIPPMAISVGYSILGRSITRLALT
GDSITAEEAKTIGLVDIVVKREDLYTEVEKQLEKIKSIDMNSIMTMKYWLKNDKEKIRNAVMELALMSLGDSAKKRMNEF
INRKRTR

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=203, Percent_Identity=37.9310344827586, Blast_Score=117, Evalue=6e-27,
Organism=Homo sapiens, GI260274832, Length=240, Percent_Identity=28.3333333333333, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI45643119, Length=240, Percent_Identity=28.3333333333333, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI260275230, Length=240, Percent_Identity=28.3333333333333, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI31542718, Length=190, Percent_Identity=26.3157894736842, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI70995211, Length=194, Percent_Identity=29.3814432989691, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI37594469, Length=210, Percent_Identity=27.6190476190476, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI37594471, Length=210, Percent_Identity=27.6190476190476, Blast_Score=71, Evalue=7e-13,
Organism=Homo sapiens, GI20127408, Length=184, Percent_Identity=29.8913043478261, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1787659, Length=222, Percent_Identity=36.4864864864865, Blast_Score=120, Evalue=7e-29,
Organism=Escherichia coli, GI221142681, Length=201, Percent_Identity=33.3333333333333, Blast_Score=98, Evalue=4e-22,
Organism=Escherichia coli, GI1787660, Length=206, Percent_Identity=36.8932038834951, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI1790281, Length=180, Percent_Identity=32.2222222222222, Blast_Score=80, Evalue=9e-17,
Organism=Escherichia coli, GI87082183, Length=185, Percent_Identity=30.2702702702703, Blast_Score=71, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17554946, Length=189, Percent_Identity=37.5661375661376, Blast_Score=119, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI25145438, Length=182, Percent_Identity=38.4615384615385, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17535521, Length=221, Percent_Identity=29.8642533936652, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17549921, Length=246, Percent_Identity=28.8617886178862, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI25144160, Length=198, Percent_Identity=31.3131313131313, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI25144157, Length=198, Percent_Identity=31.3131313131313, Blast_Score=81, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI25141363, Length=216, Percent_Identity=27.7777777777778, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17558304, Length=234, Percent_Identity=29.4871794871795, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17560910, Length=173, Percent_Identity=31.2138728323699, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17536985, Length=212, Percent_Identity=27.8301886792453, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17540714, Length=201, Percent_Identity=25.8706467661692, Blast_Score=66, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17534483, Length=193, Percent_Identity=26.9430051813472, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=240, Percent_Identity=26.6666666666667, Blast_Score=71, Evalue=1e-13,
Organism=Drosophila melanogaster, GI20129971, Length=191, Percent_Identity=39.7905759162304, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24653477, Length=191, Percent_Identity=39.7905759162304, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24654903, Length=217, Percent_Identity=29.0322580645161, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19922422, Length=198, Percent_Identity=29.2929292929293, Blast_Score=84, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24650670, Length=230, Percent_Identity=29.1304347826087, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI28571730, Length=136, Percent_Identity=30.8823529411765, Blast_Score=77, Evalue=9e-15,
Organism=Drosophila melanogaster, GI28571729, Length=136, Percent_Identity=30.8823529411765, Blast_Score=77, Evalue=9e-15,
Organism=Drosophila melanogaster, GI21357171, Length=236, Percent_Identity=27.1186440677966, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI19920382, Length=201, Percent_Identity=27.363184079602, Blast_Score=68, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24653139, Length=201, Percent_Identity=27.363184079602, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 27608; Mature: 27608

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKILSERRKDVCWITLNRPEKLNALDKESWSLLAKHLRDCNDDQSISAIVLTGNGRAFS
CCCCHHCCCCCEEEEEECCCHHCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCEEEC
AGDDINAMLELKDQRDALDFFNALYSAIESLVDLKKPLLCAVNGLAYGGGCEILLFCDVI
CCCCCHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCCCEEEEEEHH
ISVDDATFSIPEGKLGLIPPMAISVGYSILGRSITRLALTGDSITAEEAKTIGLVDIVVK
HEECCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHEEH
REDLYTEVEKQLEKIKSIDMNSIMTMKYWLKNDKEKIRNAVMELALMSLGDSAKKRMNEF
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHH
INRKRTR
HHHCCCC
>Mature Secondary Structure
MVKILSERRKDVCWITLNRPEKLNALDKESWSLLAKHLRDCNDDQSISAIVLTGNGRAFS
CCCCHHCCCCCEEEEEECCCHHCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCEEEC
AGDDINAMLELKDQRDALDFFNALYSAIESLVDLKKPLLCAVNGLAYGGGCEILLFCDVI
CCCCCHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCCCEEEEEEHH
ISVDDATFSIPEGKLGLIPPMAISVGYSILGRSITRLALTGDSITAEEAKTIGLVDIVVK
HEECCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHEEH
REDLYTEVEKQLEKIKSIDMNSIMTMKYWLKNDKEKIRNAVMELALMSLGDSAKKRMNEF
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHH
INRKRTR
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]