Click here to switch to the map view.

The map label for this gene is 227826751

Identifier: 227826751

GI number: 227826751

Start: 307254

End: 308768

Strand: Direct

Name: 227826751

Synonym: M1425_0352

Alternate gene names: NA

Gene position: 307254-308768 (Clockwise)

Preceding gene: 227826750

Following gene: 227826753

Centisome position: 11.78

GC content: 39.47

Gene sequence:

>1515_bases
ATGGGAATTAAGTTACCCACATTGGAGGATTTAAGAGAGATTTCTAGACAATTTAATTTAGATCTAGAGGATGAGGAGTT
AAAATCCTTTTTGTCATTAGTAAAACTGCAGTTAGAATCCTATGAGAGGTTAGACTCCTTACCAGATTATACTCCAAGAG
TTAAATATGAGAGAACATTAGGTCGACCACCTGTGAAAGAGGAGAATCCTTATGGTGCTTTAGTGTGGGTAACTTCAATA
AAGGGTAAGGAAGAGGGTAAGTTAAGGGGAAAAAGGATTTGCATTAAGGACAATGTGATGGTAGCGGGTATACCTATGCT
TAACGGATCAAAAATGTTAGAAGGTTTTGTCCCGAATATGGATGCTACTGTAGTTTCAAGGATTCTGGATGAGGCTGGAG
AAATCGTAGCTAAGACTACGTGTGAGGATCTATGCTTCTCAGGAGGTAGTCATACGTCTTATCCTTGGCCAGTACTAAAT
CCGAGAAATCCCGAATATATGGCAGGAGGATCATCAAGCGGTAGTGCTGTAGCAGTAGCTTCTGGCTATTGTGATATGGC
TGTTGGAGGGGATCAAGGAGGTTCAATTAGAATTCCCAGTTCGTGGGTTGGGATATATGGACTAAAACCGACTTATGGAC
TAGTTCCATATACTGGTGCTTTCTCTATCGAGCCCACATTAGATCACCTAGGACCTATGGCTAATACGGTAAGGGATATA
GCATTACTTTTGGAGGTAATAGCGGGAAGAGACGGATTAGATCCAAGACAGCCAGATAGTTTACCTCAACCTCCGGTTAA
ACCATATTTGAACCTTATTGATGGCGAGATAAAGGGTATAAAAGTAGGTCTAGTTAAGGAAGGGTTTAATTGGCCAAATT
CGGAAAAGGACGTTGATGAGTTAGTGTTGGATTCTGCTAAGAAGTTAGAAGATTATGGGATTAAGGTAGAAGAGGTCTCC
ATACCTCTTCATAGAATGGGATTGGATATATGGACTCCCATTGGCATTGAGGGTGCTACAGCCACTATGATATTGGGAAG
TGGGCTAGGATGGGGAAGGAAGGGTTTATTTGAGACCCAAATTGCCGATTTGTTTGGGAACTCCTTAAAATCCAAAGCTA
GAGATCTTCCTAATACTGTAAAGGGCGTATTAATGCTAGGTTATTTCATGATAAGGGAATATAATAATAGGTATTATGCT
AAGGCTAGAAATCTATCAATAATGTTAAAGGAAGCTTATGATGAAGCATTAAGGAAATATGATGCCCTCATAATTCCAAC
AACTCCTATGAAAGCCATGAGGTATAGGAGTGAACCAAGTTTTGATGAGTATTTCATAATGGCATTAGGAATGATAAATA
ATACCGCTCCTTATGACGTTACTGGACACCCTGCTATGAATGTACCTGTGGGATATTCGCATGGTTTACCAGTTGGGTTA
ATGATAGTAGGAAGCTACTTTGAAGAGGACAAGATACTTAAAATAGCTAATATGTTTGAAAGAATGAAAAAATAA

Upstream 100 bases:

>100_bases
TTTTTCTTCACACGGTTACAAACTCAGATCTGTTAGAGGCGAGGCATGTAATGCCACTTTTAAAACTTTTGTTTATAAGA
TTGCATTGGTAAAATATTTT

Downstream 100 bases:

>100_bases
TTTAAAAAAGATCAATTCATATTATGTCGTTTTAACTTCTTTATAAGTGAACCACCAATCAAAGGGTTGACCTAATCTCA
TTTTGGCATCTTTTATAGTT

Product: amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 504; Mature: 503

Protein sequence:

>504_residues
MGIKLPTLEDLREISRQFNLDLEDEELKSFLSLVKLQLESYERLDSLPDYTPRVKYERTLGRPPVKEENPYGALVWVTSI
KGKEEGKLRGKRICIKDNVMVAGIPMLNGSKMLEGFVPNMDATVVSRILDEAGEIVAKTTCEDLCFSGGSHTSYPWPVLN
PRNPEYMAGGSSSGSAVAVASGYCDMAVGGDQGGSIRIPSSWVGIYGLKPTYGLVPYTGAFSIEPTLDHLGPMANTVRDI
ALLLEVIAGRDGLDPRQPDSLPQPPVKPYLNLIDGEIKGIKVGLVKEGFNWPNSEKDVDELVLDSAKKLEDYGIKVEEVS
IPLHRMGLDIWTPIGIEGATATMILGSGLGWGRKGLFETQIADLFGNSLKSKARDLPNTVKGVLMLGYFMIREYNNRYYA
KARNLSIMLKEAYDEALRKYDALIIPTTPMKAMRYRSEPSFDEYFIMALGMINNTAPYDVTGHPAMNVPVGYSHGLPVGL
MIVGSYFEEDKILKIANMFERMKK

Sequences:

>Translated_504_residues
MGIKLPTLEDLREISRQFNLDLEDEELKSFLSLVKLQLESYERLDSLPDYTPRVKYERTLGRPPVKEENPYGALVWVTSI
KGKEEGKLRGKRICIKDNVMVAGIPMLNGSKMLEGFVPNMDATVVSRILDEAGEIVAKTTCEDLCFSGGSHTSYPWPVLN
PRNPEYMAGGSSSGSAVAVASGYCDMAVGGDQGGSIRIPSSWVGIYGLKPTYGLVPYTGAFSIEPTLDHLGPMANTVRDI
ALLLEVIAGRDGLDPRQPDSLPQPPVKPYLNLIDGEIKGIKVGLVKEGFNWPNSEKDVDELVLDSAKKLEDYGIKVEEVS
IPLHRMGLDIWTPIGIEGATATMILGSGLGWGRKGLFETQIADLFGNSLKSKARDLPNTVKGVLMLGYFMIREYNNRYYA
KARNLSIMLKEAYDEALRKYDALIIPTTPMKAMRYRSEPSFDEYFIMALGMINNTAPYDVTGHPAMNVPVGYSHGLPVGL
MIVGSYFEEDKILKIANMFERMKK
>Mature_503_residues
GIKLPTLEDLREISRQFNLDLEDEELKSFLSLVKLQLESYERLDSLPDYTPRVKYERTLGRPPVKEENPYGALVWVTSIK
GKEEGKLRGKRICIKDNVMVAGIPMLNGSKMLEGFVPNMDATVVSRILDEAGEIVAKTTCEDLCFSGGSHTSYPWPVLNP
RNPEYMAGGSSSGSAVAVASGYCDMAVGGDQGGSIRIPSSWVGIYGLKPTYGLVPYTGAFSIEPTLDHLGPMANTVRDIA
LLLEVIAGRDGLDPRQPDSLPQPPVKPYLNLIDGEIKGIKVGLVKEGFNWPNSEKDVDELVLDSAKKLEDYGIKVEEVSI
PLHRMGLDIWTPIGIEGATATMILGSGLGWGRKGLFETQIADLFGNSLKSKARDLPNTVKGVLMLGYFMIREYNNRYYAK
ARNLSIMLKEAYDEALRKYDALIIPTTPMKAMRYRSEPSFDEYFIMALGMINNTAPYDVTGHPAMNVPVGYSHGLPVGLM
IVGSYFEEDKILKIANMFERMKK

Specific function: Enantioselective, active on 2-to 6-carbon aliphatic amides and on many aromatic amides [H]

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=451, Percent_Identity=28.8248337028825, Blast_Score=164, Evalue=3e-40,
Organism=Homo sapiens, GI195972892, Length=443, Percent_Identity=24.6049661399549, Blast_Score=95, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17543272, Length=423, Percent_Identity=25.7683215130024, Blast_Score=152, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17537465, Length=457, Percent_Identity=26.2582056892779, Blast_Score=91, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323950, Length=482, Percent_Identity=29.6680497925311, Blast_Score=121, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24648113, Length=428, Percent_Identity=29.9065420560748, Blast_Score=143, Evalue=3e-34,
Organism=Drosophila melanogaster, GI161078093, Length=435, Percent_Identity=27.816091954023, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24644968, Length=423, Percent_Identity=22.9314420803783, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI45550774, Length=442, Percent_Identity=24.8868778280543, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24648435, Length=442, Percent_Identity=24.8868778280543, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24648437, Length=442, Percent_Identity=24.8868778280543, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24648441, Length=441, Percent_Identity=24.2630385487528, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24648439, Length=441, Percent_Identity=24.2630385487528, Blast_Score=73, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.1.4 [H]

Molecular weight: Translated: 55742; Mature: 55611

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIKLPTLEDLREISRQFNLDLEDEELKSFLSLVKLQLESYERLDSLPDYTPRVKYERTL
CCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHC
GRPPVKEENPYGALVWVTSIKGKEEGKLRGKRICIKDNVMVAGIPMLNGSKMLEGFVPNM
CCCCCCCCCCCEEEEEEEECCCCCCCCCCCCEEEEECCEEEEEEECCCCCHHHHHCCCCC
DATVVSRILDEAGEIVAKTTCEDLCFSGGSHTSYPWPVLNPRNPEYMAGGSSSGSAVAVA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECCCCCCCEEEEE
SGYCDMAVGGDQGGSIRIPSSWVGIYGLKPTYGLVPYTGAFSIEPTLDHLGPMANTVRDI
CCCEEEEECCCCCCEEECCCCCEEEEECCCCCCCCCCCCCEECCCCHHHHCHHHHHHHHH
ALLLEVIAGRDGLDPRQPDSLPQPPVKPYLNLIDGEIKGIKVGLVKEGFNWPNSEKDVDE
HHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCHHHH
LVLDSAKKLEDYGIKVEEVSIPLHRMGLDIWTPIGIEGATATMILGSGLGWGRKGLFETQ
HHHHHHHHHHHHCCEEEEEECCHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHH
IADLFGNSLKSKARDLPNTVKGVLMLGYFMIREYNNRYYAKARNLSIMLKEAYDEALRKY
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEHHHHHHHHHHHHH
DALIIPTTPMKAMRYRSEPSFDEYFIMALGMINNTAPYDVTGHPAMNVPVGYSHGLPVGL
CCEEECCCCHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEE
MIVGSYFEEDKILKIANMFERMKK
EEEECCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
GIKLPTLEDLREISRQFNLDLEDEELKSFLSLVKLQLESYERLDSLPDYTPRVKYERTL
CCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHC
GRPPVKEENPYGALVWVTSIKGKEEGKLRGKRICIKDNVMVAGIPMLNGSKMLEGFVPNM
CCCCCCCCCCCEEEEEEEECCCCCCCCCCCCEEEEECCEEEEEEECCCCCHHHHHCCCCC
DATVVSRILDEAGEIVAKTTCEDLCFSGGSHTSYPWPVLNPRNPEYMAGGSSSGSAVAVA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECCCCCCCEEEEE
SGYCDMAVGGDQGGSIRIPSSWVGIYGLKPTYGLVPYTGAFSIEPTLDHLGPMANTVRDI
CCCEEEEECCCCCCEEECCCCCEEEEECCCCCCCCCCCCCEECCCCHHHHCHHHHHHHHH
ALLLEVIAGRDGLDPRQPDSLPQPPVKPYLNLIDGEIKGIKVGLVKEGFNWPNSEKDVDE
HHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCHHHH
LVLDSAKKLEDYGIKVEEVSIPLHRMGLDIWTPIGIEGATATMILGSGLGWGRKGLFETQ
HHHHHHHHHHHHCCEEEEEECCHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHH
IADLFGNSLKSKARDLPNTVKGVLMLGYFMIREYNNRYYAKARNLSIMLKEAYDEALRKY
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEHHHHHHHHHHHHH
DALIIPTTPMKAMRYRSEPSFDEYFIMALGMINNTAPYDVTGHPAMNVPVGYSHGLPVGL
CCEEECCCCHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEE
MIVGSYFEEDKILKIANMFERMKK
EEEECCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11453462; 8899719; 11427726 [H]