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The map label for this gene is clcE [H]

Identifier: 227826690

GI number: 227826690

Start: 245227

End: 246210

Strand: Direct

Name: clcE [H]

Synonym: M1425_0282

Alternate gene names: 227826690

Gene position: 245227-246210 (Clockwise)

Preceding gene: 227826689

Following gene: 227826691

Centisome position: 9.4

GC content: 36.69

Gene sequence:

>984_bases
ATGTTCAAAGTAGAGTACCCTATTACACAAGTAATTTATGGACTTAACGCACTAGATTGGTTAGCAAGTGTGAAGGGGAA
AAGGATAGCTTTAGTCACTACTAGGAGCTTGCTTAAAAGTAAAATTCTTGAACAAATATTAAGTCTTATAAACGCTGAGG
TAATTGAGGGACCAAGACAACACACTCCAGTAGGTGATGTAAATAATTTAACGGAGAGACTTAAGGGATATGATGTTGTA
ATAGGCTTAGGCGGAGGAAGTGTAATAGATGGTATTAAGCTTTCATTTAATGGTTACTATATAGCAATACCTACTACTTT
TTCTGGAGCGGAGCATACTAGATCTGGAGGAGCAACAATTGATGGAATAAAGAAAAGTAGAATAGGAAAAGAGGCAGATG
CAATAATCTTAGATCCTAGAGCCACACTTGAAACACCAAAATGGTTGCTTATAGCAAGTGGAGTAAGGGCAATAGACCAC
GCGGTCGAGGCATTATACTCTAAAGACTCTACACCCTTTACTGATTCCTTAGCTATAGAGGGTTATAAAAAGCTAGTTAA
ATGCATAAGAGATCTAGATTCAATTGAAAATAGGGCATTATGCCAAATAGGCACGTGGTTATCGTCATTAACGATGAGAT
ATGCTAAAATGGGGATAAGTCATAATTTCGGTTACGTTTATGGTCCTAGATTTAATATACCTCACGGAGTAACCTCTTGT
ATCTCATTGCCATCAGCAATTAAGTTAAACTATAGTGTTGCCAAAAATAAATTGAGGGAGATAGAGAATGAAGGTGAGCC
ATTATACGAGTTCATGGATAAATTCCTAAAGGATATCGGAGCTAAGCGGAGACTATCTGAATTTACTACCTTGGATGAGG
CATTAAAATATGTGCAAACTTTCGTTGAAATTGTGAACAATAGTGGAAATCCGGTTAGAATTGACATAGAAACAGCTAAG
AGGTTTATAGAAGAGGTCTTTTAA

Upstream 100 bases:

>100_bases
TTTCACAATCTTACCATGCTTAATAGACTAATCAAGAAACTATCATTTACCATGTAGAGTTTTGTAAGTTTATTAATTTA
TTTGTCATACATTAATACAT

Downstream 100 bases:

>100_bases
AATGATAGAGTGGAAGGGCTTTGGAAAAAGATGGGGTAAGTGTGAAGAGTGTTGGTTGGCTTATGAAAGGCGAATTCAAC
ATGAAAATTCTTTGAACTGT

Product: iron-containing alcohol dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MFKVEYPITQVIYGLNALDWLASVKGKRIALVTTRSLLKSKILEQILSLINAEVIEGPRQHTPVGDVNNLTERLKGYDVV
IGLGGGSVIDGIKLSFNGYYIAIPTTFSGAEHTRSGGATIDGIKKSRIGKEADAIILDPRATLETPKWLLIASGVRAIDH
AVEALYSKDSTPFTDSLAIEGYKKLVKCIRDLDSIENRALCQIGTWLSSLTMRYAKMGISHNFGYVYGPRFNIPHGVTSC
ISLPSAIKLNYSVAKNKLREIENEGEPLYEFMDKFLKDIGAKRRLSEFTTLDEALKYVQTFVEIVNNSGNPVRIDIETAK
RFIEEVF

Sequences:

>Translated_327_residues
MFKVEYPITQVIYGLNALDWLASVKGKRIALVTTRSLLKSKILEQILSLINAEVIEGPRQHTPVGDVNNLTERLKGYDVV
IGLGGGSVIDGIKLSFNGYYIAIPTTFSGAEHTRSGGATIDGIKKSRIGKEADAIILDPRATLETPKWLLIASGVRAIDH
AVEALYSKDSTPFTDSLAIEGYKKLVKCIRDLDSIENRALCQIGTWLSSLTMRYAKMGISHNFGYVYGPRFNIPHGVTSC
ISLPSAIKLNYSVAKNKLREIENEGEPLYEFMDKFLKDIGAKRRLSEFTTLDEALKYVQTFVEIVNNSGNPVRIDIETAK
RFIEEVF
>Mature_327_residues
MFKVEYPITQVIYGLNALDWLASVKGKRIALVTTRSLLKSKILEQILSLINAEVIEGPRQHTPVGDVNNLTERLKGYDVV
IGLGGGSVIDGIKLSFNGYYIAIPTTFSGAEHTRSGGATIDGIKKSRIGKEADAIILDPRATLETPKWLLIASGVRAIDH
AVEALYSKDSTPFTDSLAIEGYKKLVKCIRDLDSIENRALCQIGTWLSSLTMRYAKMGISHNFGYVYGPRFNIPHGVTSC
ISLPSAIKLNYSVAKNKLREIENEGEPLYEFMDKFLKDIGAKRRLSEFTTLDEALKYVQTFVEIVNNSGNPVRIDIETAK
RFIEEVF

Specific function: May Act On The Acetaldehyde Produced From The Degradation Of Ethanolamine. [C]

COG id: COG1454

COG function: function code C; Alcohol dehydrogenase, class IV

Gene ontology:

Cell location: Integral Membrane Protein [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI87082107, Length=305, Percent_Identity=26.8852459016393, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI48994951, Length=284, Percent_Identity=27.4647887323944, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: =1.3.1.32 [H]

Molecular weight: Translated: 36310; Mature: 36310

Theoretical pI: Translated: 8.69; Mature: 8.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKVEYPITQVIYGLNALDWLASVKGKRIALVTTRSLLKSKILEQILSLINAEVIEGPRQ
CEEECCCHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCC
HTPVGDVNNLTERLKGYDVVIGLGGGSVIDGIKLSFNGYYIAIPTTFSGAEHTRSGGATI
CCCCCCHHHHHHHHCCCEEEEECCCCCCCCCEEEEECCEEEEEECCCCCCCCCCCCCCCC
DGIKKSRIGKEADAIILDPRATLETPKWLLIASGVRAIDHAVEALYSKDSTPFTDSLAIE
CCHHHHHCCCCCCEEEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
GYKKLVKCIRDLDSIENRALCQIGTWLSSLTMRYAKMGISHNFGYVYGPRFNIPHGVTSC
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHH
ISLPSAIKLNYSVAKNKLREIENEGEPLYEFMDKFLKDIGAKRRLSEFTTLDEALKYVQT
HCCCCHHEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FVEIVNNSGNPVRIDIETAKRFIEEVF
HHHHHCCCCCEEEEEHHHHHHHHHHHC
>Mature Secondary Structure
MFKVEYPITQVIYGLNALDWLASVKGKRIALVTTRSLLKSKILEQILSLINAEVIEGPRQ
CEEECCCHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCC
HTPVGDVNNLTERLKGYDVVIGLGGGSVIDGIKLSFNGYYIAIPTTFSGAEHTRSGGATI
CCCCCCHHHHHHHHCCCEEEEECCCCCCCCCEEEEECCEEEEEECCCCCCCCCCCCCCCC
DGIKKSRIGKEADAIILDPRATLETPKWLLIASGVRAIDHAVEALYSKDSTPFTDSLAIE
CCHHHHHCCCCCCEEEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
GYKKLVKCIRDLDSIENRALCQIGTWLSSLTMRYAKMGISHNFGYVYGPRFNIPHGVTSC
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHH
ISLPSAIKLNYSVAKNKLREIENEGEPLYEFMDKFLKDIGAKRRLSEFTTLDEALKYVQT
HCCCCHHEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FVEIVNNSGNPVRIDIETAKRFIEEVF
HHHHHCCCCCEEEEEHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11571162 [H]