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The map label for this gene is cobB [H]

Identifier: 227826676

GI number: 227826676

Start: 233284

End: 234027

Strand: Direct

Name: cobB [H]

Synonym: M1425_0267

Alternate gene names: 227826676

Gene position: 233284-234027 (Clockwise)

Preceding gene: 227826675

Following gene: 227826677

Centisome position: 8.94

GC content: 38.58

Gene sequence:

>744_bases
ATGATATACGAGAAAGTAGCTGAAGAACTTATTTCCTCTTCTTACGCCATAGCCTTTACCGGAGCCGGTATCAGTACAGC
TTCTGGTATTCCCGATTTCAGAGGTCCTCAAGGATTATGGAAGAAGTATTCACCAGAGTTAGCTAGTATAGAATATTTTG
AAAAAGATCCTAAAAATTTTTGGGGATTCTACTCATTAAGAATGAGGGGATTATTCGAAGCCCAACCTAATAAGGCACAC
TACTCTTTGGCTGAGTTAGAAAAGATGGGCATAATAAAGGTAATAATAACACAAAATATTGACGGTCTACATCAGAAGGC
TGGTTCTAAGAACGTTATTGAGCTTCACGGAACTATGAGGAGGTCATATTGCGTTTTATGCTTAAGGACTTATGATTCGC
TAAACGTCCTAAGTATGATAGAAAAGGGTAATCTTCCACCTAGGTGTGATTGTGGTGGGATAATAAGGCCAGATGTTGTG
CTTTTTGGGGAACCAGTTAAGAACATTTATGAGGCACTAAGTATTGCCTATGAATCTGATCTCGTAATCTCAATTGGTTC
TTCATTAACTGTATATCCAGCCAATTTAATTCCACAGACCGTAAAAGAAAGAGGTGGTAAGCTAATAATTTTAAATATGG
AGGAGACCCCGTTGGATAGTATTGCGGATTACGTTGTAAGGGAACCAGTGGAAATTTCATTGCCCAAGATAGTTGAGAAC
GTGAGACAGAAAATACTTTCGTGA

Upstream 100 bases:

>100_bases
TAAAAGCAATATATCTGCAGAGGATCTAAAAGGATGAAAGTTGAACTCAAACCTTTTTTATTTTAAAATCTTATTTACCT
ACTAGTAGTATATTCTAATT

Downstream 100 bases:

>100_bases
ATCTCAGTAGCGTTTTTTAAGTAAAATTTCTGATATGATTGAAGAGGAAGAGGAATTAAAATTAATAAAACAAATATTTT
TAATAATTAATGAGAATGTG

Product: NAD-dependent deacetylase

Products: NA

Alternate protein names: Regulatory protein SIR2 homolog; ssSir2 [H]

Number of amino acids: Translated: 247; Mature: 247

Protein sequence:

>247_residues
MIYEKVAEELISSSYAIAFTGAGISTASGIPDFRGPQGLWKKYSPELASIEYFEKDPKNFWGFYSLRMRGLFEAQPNKAH
YSLAELEKMGIIKVIITQNIDGLHQKAGSKNVIELHGTMRRSYCVLCLRTYDSLNVLSMIEKGNLPPRCDCGGIIRPDVV
LFGEPVKNIYEALSIAYESDLVISIGSSLTVYPANLIPQTVKERGGKLIILNMEETPLDSIADYVVREPVEISLPKIVEN
VRQKILS

Sequences:

>Translated_247_residues
MIYEKVAEELISSSYAIAFTGAGISTASGIPDFRGPQGLWKKYSPELASIEYFEKDPKNFWGFYSLRMRGLFEAQPNKAH
YSLAELEKMGIIKVIITQNIDGLHQKAGSKNVIELHGTMRRSYCVLCLRTYDSLNVLSMIEKGNLPPRCDCGGIIRPDVV
LFGEPVKNIYEALSIAYESDLVISIGSSLTVYPANLIPQTVKERGGKLIILNMEETPLDSIADYVVREPVEISLPKIVEN
VRQKILS
>Mature_247_residues
MIYEKVAEELISSSYAIAFTGAGISTASGIPDFRGPQGLWKKYSPELASIEYFEKDPKNFWGFYSLRMRGLFEAQPNKAH
YSLAELEKMGIIKVIITQNIDGLHQKAGSKNVIELHGTMRRSYCVLCLRTYDSLNVLSMIEKGNLPPRCDCGGIIRPDVV
LFGEPVKNIYEALSIAYESDLVISIGSSLTVYPANLIPQTVKERGGKLIILNMEETPLDSIADYVVREPVEISLPKIVEN
VRQKILS

Specific function: Probably has a role in chromatin modulation by deacetylation of the N-terminal lysine residue of Alba, the major archaeal chromatin protein and that, in turn, increases Alba's DNA binding affinity, thereby repressing transcription [H]

COG id: COG0846

COG function: function code K; NAD-dependent protein deacetylases, SIR2 family

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 deacetylase sirtuin-type domain [H]

Homologues:

Organism=Homo sapiens, GI6912664, Length=252, Percent_Identity=33.7301587301587, Blast_Score=141, Evalue=5e-34,
Organism=Homo sapiens, GI300795542, Length=239, Percent_Identity=34.3096234309623, Blast_Score=140, Evalue=1e-33,
Organism=Homo sapiens, GI13787215, Length=231, Percent_Identity=35.4978354978355, Blast_Score=137, Evalue=8e-33,
Organism=Homo sapiens, GI300797577, Length=254, Percent_Identity=32.2834645669291, Blast_Score=123, Evalue=1e-28,
Organism=Homo sapiens, GI300797597, Length=242, Percent_Identity=30.9917355371901, Blast_Score=115, Evalue=3e-26,
Organism=Homo sapiens, GI7657575, Length=220, Percent_Identity=36.3636363636364, Blast_Score=111, Evalue=5e-25,
Organism=Homo sapiens, GI6912662, Length=243, Percent_Identity=31.6872427983539, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI63054862, Length=202, Percent_Identity=32.6732673267327, Blast_Score=108, Evalue=6e-24,
Organism=Homo sapiens, GI6912660, Length=206, Percent_Identity=32.0388349514563, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI7706712, Length=235, Percent_Identity=31.063829787234, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI13775602, Length=199, Percent_Identity=36.1809045226131, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI13775600, Length=199, Percent_Identity=36.1809045226131, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI300797705, Length=190, Percent_Identity=35.2631578947368, Blast_Score=94, Evalue=9e-20,
Organism=Homo sapiens, GI215982798, Length=181, Percent_Identity=35.9116022099448, Blast_Score=89, Evalue=3e-18,
Organism=Escherichia coli, GI308199517, Length=237, Percent_Identity=37.5527426160338, Blast_Score=127, Evalue=9e-31,
Organism=Caenorhabditis elegans, GI17541892, Length=219, Percent_Identity=31.5068493150685, Blast_Score=116, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17567771, Length=239, Percent_Identity=33.0543933054393, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71990482, Length=244, Percent_Identity=30.7377049180328, Blast_Score=98, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI71990487, Length=246, Percent_Identity=30.4878048780488, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17505510, Length=226, Percent_Identity=28.3185840707965, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6325242, Length=247, Percent_Identity=30.7692307692308, Blast_Score=104, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24645650, Length=250, Percent_Identity=30.4, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24648389, Length=189, Percent_Identity=37.037037037037, Blast_Score=106, Evalue=1e-23,
Organism=Drosophila melanogaster, GI28571445, Length=277, Percent_Identity=28.8808664259928, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI17137536, Length=234, Percent_Identity=30.7692307692308, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24650933, Length=236, Percent_Identity=27.9661016949153, Blast_Score=84, Evalue=9e-17,
Organism=Drosophila melanogaster, GI28571443, Length=219, Percent_Identity=28.310502283105, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI28571441, Length=219, Percent_Identity=28.310502283105, Blast_Score=83, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003000 [H]

Pfam domain/function: PF02146 SIR2 [H]

EC number: 3.5.1.- [C]

Molecular weight: Translated: 27550; Mature: 27550

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS50305 SIRTUIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYEKVAEELISSSYAIAFTGAGISTASGIPDFRGPQGLWKKYSPELASIEYFEKDPKNF
CCHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHCCCCHH
WGFYSLRMRGLFEAQPNKAHYSLAELEKMGIIKVIITQNIDGLHQKAGSKNVIELHGTMR
HHHHHHHHHHHCCCCCCCHHHHHHHHHHCCEEEEEEECCCCHHHHHCCCCCEEEEHHHHH
RSYCVLCLRTYDSLNVLSMIEKGNLPPRCDCGGIIRPDVVLFGEPVKNIYEALSIAYESD
HHHHHHEEHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHCCC
LVISIGSSLTVYPANLIPQTVKERGGKLIILNMEETPLDSIADYVVREPVEISLPKIVEN
EEEEECCCEEEEEHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCCCCCCHHHHHHH
VRQKILS
HHHHHCC
>Mature Secondary Structure
MIYEKVAEELISSSYAIAFTGAGISTASGIPDFRGPQGLWKKYSPELASIEYFEKDPKNF
CCHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHCCCCHH
WGFYSLRMRGLFEAQPNKAHYSLAELEKMGIIKVIITQNIDGLHQKAGSKNVIELHGTMR
HHHHHHHHHHHCCCCCCCHHHHHHHHHHCCEEEEEEECCCCHHHHHCCCCCEEEEHHHHH
RSYCVLCLRTYDSLNVLSMIEKGNLPPRCDCGGIIRPDVVLFGEPVKNIYEALSIAYESD
HHHHHHEEHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHCCC
LVISIGSSLTVYPANLIPQTVKERGGKLIILNMEETPLDSIADYVVREPVEISLPKIVEN
EEEEECCCEEEEEHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCCCCCCHHHHHHH
VRQKILS
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11427726; 11935028 [H]