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The map label for this gene is ybiK [C]

Identifier: 227826557

GI number: 227826557

Start: 129011

End: 129841

Strand: Direct

Name: ybiK [C]

Synonym: M1425_0147

Alternate gene names: 227826557

Gene position: 129011-129841 (Clockwise)

Preceding gene: 227826553

Following gene: 227826558

Centisome position: 4.95

GC content: 39.71

Gene sequence:

>831_bases
GTGAGATATAATTTACCAGTACTCGTAATACACGGCGGAGCAGGAAGTTGGCAAATAGCTGATCAAGATAAGGCGAAACT
GACAATAAGCGAAGCGTTAGAGAGAGGATATTATGAGTTTAGGAAAGGTTCTGCGCTTGAAGCCGTAGTGGAGGCCATAT
ACTATATGGAGGAGTCTGGAGTTTTCGATGCAGGAAAAGGTAGTGTAAGAAACTCAGCTGGATATATTGAAATGGACGCT
GGAATAATGATAGGTAATACACTTCAAGCGGGGGGTATTATGGGATTGAGAGAAGGTAGCGCGATAAAAAAGGCTTTTGA
AATCTTACTTCAAAATAGGCATGTATTAATGATAGGTAGTGGCGATAATACTTCTAATAATAATAAGAATGTAATTTCCG
AAAGTAAGGTTTCCGGAGATACTGTAGGTGCAGTAGCTCTAGACCAACATGGTAATCTAGTTGCCGGAACTAGCACTGGT
GGGATAAAGGGAAAATTACCGGGTAGAGTTGGGGATTCTCCTATTCCGGGCGCAGGCTATTACGCAACGCCGAATGTGGC
TGTTTCTAGTACCGGAGTTGGTGAGATAATTTTAAGGATGTTACCAGCTAAAGAAGTTGATATTTTAGTCTCATTGGGTT
TCACTATTGATGATGCACTAAGAGCTGTGATAAACAAAATAACTAAAATTTTTGGAAAAGATAATATAGGAATGATAGGA
TTAGATAAATATGGAAATGCCTCGGCTTACTATAATACAAAAGGGATGGCTAGAGGTGTCATTTCCTCAGATGGAGTTAA
GAAAGTATATGTCTTCGAGGGTGAGATCTAA

Upstream 100 bases:

>100_bases
CTATCATCAATATGGAAAGGGGTAACGTATAACTGGCTTTCATCTTATTCTTAAAGACTACTAATTAGAAAATAAAAATA
TCTATTCCCAATAGAAGAGT

Downstream 100 bases:

>100_bases
ATGAAGATATTAGTAATGAGCAATATTAGATTCCCCGAACCTCATGTTGAAAGTATGTTATCAAGTATTATAAAGAAGGA
GGAGCCAGAAGTTATAGTAT

Product: peptidase T2 asparaginase 2

Products: NA

Alternate protein names: L-asparagine amidohydrolase [H]

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MRYNLPVLVIHGGAGSWQIADQDKAKLTISEALERGYYEFRKGSALEAVVEAIYYMEESGVFDAGKGSVRNSAGYIEMDA
GIMIGNTLQAGGIMGLREGSAIKKAFEILLQNRHVLMIGSGDNTSNNNKNVISESKVSGDTVGAVALDQHGNLVAGTSTG
GIKGKLPGRVGDSPIPGAGYYATPNVAVSSTGVGEIILRMLPAKEVDILVSLGFTIDDALRAVINKITKIFGKDNIGMIG
LDKYGNASAYYNTKGMARGVISSDGVKKVYVFEGEI

Sequences:

>Translated_276_residues
MRYNLPVLVIHGGAGSWQIADQDKAKLTISEALERGYYEFRKGSALEAVVEAIYYMEESGVFDAGKGSVRNSAGYIEMDA
GIMIGNTLQAGGIMGLREGSAIKKAFEILLQNRHVLMIGSGDNTSNNNKNVISESKVSGDTVGAVALDQHGNLVAGTSTG
GIKGKLPGRVGDSPIPGAGYYATPNVAVSSTGVGEIILRMLPAKEVDILVSLGFTIDDALRAVINKITKIFGKDNIGMIG
LDKYGNASAYYNTKGMARGVISSDGVKKVYVFEGEI
>Mature_276_residues
MRYNLPVLVIHGGAGSWQIADQDKAKLTISEALERGYYEFRKGSALEAVVEAIYYMEESGVFDAGKGSVRNSAGYIEMDA
GIMIGNTLQAGGIMGLREGSAIKKAFEILLQNRHVLMIGSGDNTSNNNKNVISESKVSGDTVGAVALDQHGNLVAGTSTG
GIKGKLPGRVGDSPIPGAGYYATPNVAVSSTGVGEIILRMLPAKEVDILVSLGFTIDDALRAVINKITKIFGKDNIGMIG
LDKYGNASAYYNTKGMARGVISSDGVKKVYVFEGEI

Specific function: Unknown

COG id: COG1446

COG function: function code E; Asparaginase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Ntn-hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI145275202, Length=245, Percent_Identity=35.9183673469388, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI145275200, Length=245, Percent_Identity=35.9183673469388, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI285002251, Length=247, Percent_Identity=30.3643724696356, Blast_Score=76, Evalue=3e-14,
Organism=Escherichia coli, GI1787050, Length=295, Percent_Identity=36.6101694915254, Blast_Score=142, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17569239, Length=98, Percent_Identity=41.8367346938776, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI18921183, Length=324, Percent_Identity=31.1728395061728, Blast_Score=111, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24652135, Length=268, Percent_Identity=30.2238805970149, Blast_Score=87, Evalue=8e-18,
Organism=Drosophila melanogaster, GI221330102, Length=268, Percent_Identity=30.2238805970149, Blast_Score=87, Evalue=9e-18,
Organism=Drosophila melanogaster, GI24655553, Length=275, Percent_Identity=27.6363636363636, Blast_Score=79, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000246 [H]

Pfam domain/function: PF01112 Asparaginase_2 [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 29105; Mature: 29105

Theoretical pI: Translated: 6.82; Mature: 6.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYNLPVLVIHGGAGSWQIADQDKAKLTISEALERGYYEFRKGSALEAVVEAIYYMEESG
CCCCCCEEEEECCCCCEEECCCCCCEEEHHHHHHCCHHHHCCCCHHHHHHHHHHHHHCCC
VFDAGKGSVRNSAGYIEMDAGIMIGNTLQAGGIMGLREGSAIKKAFEILLQNRHVLMIGS
CCCCCCCCCCCCCCEEEECCCEEECCEECCCCEEEECCCHHHHHHHHHHHCCCCEEEEEC
GDNTSNNNKNVISESKVSGDTVGAVALDQHGNLVAGTSTGGIKGKLPGRVGDSPIPGAGY
CCCCCCCCCCCEECCCCCCCEEEEEEEECCCCEEEECCCCCCCCCCCCCCCCCCCCCCCE
YATPNVAVSSTGVGEIILRMLPAKEVDILVSLGFTIDDALRAVINKITKIFGKDNIGMIG
EECCCEEEECCCHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCEEE
LDKYGNASAYYNTKGMARGVISSDGVKKVYVFEGEI
ECCCCCCCEEECCCCCCCCCCCCCCCEEEEEEECCC
>Mature Secondary Structure
MRYNLPVLVIHGGAGSWQIADQDKAKLTISEALERGYYEFRKGSALEAVVEAIYYMEESG
CCCCCCEEEEECCCCCEEECCCCCCEEEHHHHHHCCHHHHCCCCHHHHHHHHHHHHHCCC
VFDAGKGSVRNSAGYIEMDAGIMIGNTLQAGGIMGLREGSAIKKAFEILLQNRHVLMIGS
CCCCCCCCCCCCCCEEEECCCEEECCEECCCCEEEECCCHHHHHHHHHHHCCCCEEEEEC
GDNTSNNNKNVISESKVSGDTVGAVALDQHGNLVAGTSTGGIKGKLPGRVGDSPIPGAGY
CCCCCCCCCCCEECCCCCCCEEEEEEEECCCCEEEECCCCCCCCCCCCCCCCCCCCCCCE
YATPNVAVSSTGVGEIILRMLPAKEVDILVSLGFTIDDALRAVINKITKIFGKDNIGMIG
EECCCEEEECCCHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCEEE
LDKYGNASAYYNTKGMARGVISSDGVKKVYVFEGEI
ECCCCCCCEEECCCCCCCCCCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9679194 [H]